Supplementary Tables s2

Supplementary Tables

Table S1. Effects of P and NP addition on nitrogenase activity in different compartments (forest floor, bulk soil, rhizosphere, and nodule) and on total nitrogenase activity in the EU and AA plantations, respectively.

Compartments / Forest floor
(nmol C2H4 g-1 h-1) / Bulk soil
(nmol C2H4 g-1 h-1) / Rhizosphere
(nmol C2H4 g-1 h-1) / Nodule
(nmol C2H4 g-1 h-1) / Total
(nmol C2H4 g-1 h-1)
EU / C / 0.94(0.07) / 0.54(0.01) / 0.66(0.01) / 2.14(0.09)
P / 0.98(0.07) / 0.58(0.01) / 0.75(0.02)* / 2.30(0.06)
NP / 0.86(0.03) / 0.50(0.03) / 0.75(0.01)* / 2.11(0.04)
AA / C / 1.05(0.09) / 0.43(0.01) / 0.33(0.01) / 1926.72(58.73) / 1928.52(58.65)
P / 1.13(0.02) / 0.47(0.02) / 0.31(0.01) / 2071.30(71.08) / 2073.21(71.04)
NP / 0.82(0.08)* / 0.38(0.02) / 0.28(0.01)* / 1975.73(155.57) / 1977.20(155.54)

EU: Eucalyptus urophylla; AA: Acacia auriculiformis; C: control; P: phosphorus addition; NP: combined nitrogen and phosphorus additions. Values are means with standard errors in brackets (n = 3). * represents significant difference between treatment and control within each compartment of each plantation (One-way ANOVA with Bonferroni-adjusted pairwise comparison, P < 0.05).

Table S2. Effects of P and NP addition on BNF rate in different compartments (forest floor, bulk soil, rhizosphere, and nodule) and on total BNF rate in the EU and AA plantations, respectively.

Compartments / Forest floor
(kg N ha-1 yr-1) / Bulk soil
(kg N ha-1 yr-1) / Rhizosphere
(kg N ha-1 yr-1) / Nodule
(kg N ha-1 yr-1) / Total
(kg N ha-1 yr-1)
EU / C / 0.40(0.12) / 4.17(0.18) / 1.82(0.16) / 6.39(0.41)
P / 0.47(0.04) / 4.62(0.13) / 2.98(0.11)* / 8.07(0.19)*
NP / 0.50(0.09) / 3.79(0.20) / 3.80(0.17)* / 8.09(0.36)*
AA / C / 1.05(0.14) / 3.90(0.13) / 0.52(0.04) / 0.67(0.06) / 6.14(0.11)
P / 0.96(0.13) / 3.88(0.11) / 0.63(0.06) / 2.09(0.14)* / 7.56(0.15)*
NP / 0.99(0.03) / 3.83(0.10) / 0.45(0.03) / 1.39(0.09)* / 6.65(0.12)*

Table S3. Effects of P and NP addition on DIN content (bulk soil and rhizosphere) and total N content (root and leaf) in the EU and AA plantations, respectively.

Compartments / Bulk soil DIN
(mg kg-2) / Rhizosphere DIN
(mg kg-2) / Root N
(mg g-2) / Leaf N
(mg g-2)
EU / C / 17.93(0.31) / 31.91(1.51) / 4.36(0.54) / 2.27(0.09)
P / 16.05(1.78) / 20.93(0.89)* / 6.60(0.33)* / 2.53(0.06)*
NP / 21.28(1.96) / 24.32(1.38) / 7.70(0.26)* / 2.55(0.07)*
AA / C / 24.30(1.66) / 26.87(0.57) / 12.25(0.60) / 3.22(0.10)
P / 24.29(1.18) / 27.57(1.95) / 15.96(0.64)* / 3.01(0.06)
NP / 28.54(4.28) / 30.49(1.99) / 14.74(0.32)* / 3.05(0.04)

Table S4. Effects of P and NP addition on AP content (bulk soil and rhizosphere) and total P content (root and leaf) in the EU and AA plantations, respectively.

Compartments / Bulk soil DIN
(mg kg-2) / Rhizosphere DIN
(mg kg-2) / Root N
(mg g-2) / Leaf N
(mg g-2)
EU / C / 2.31(0.30) / 2.11(0.56) / 0.36(0.02) / 0.09(0.01)
P / 12.22(1.39)* / 11.54(0.51)* / 0.86(0.05)* / 0.15(0.01)*
NP / 13.58(1.45)* / 15.10(1.80)* / 0.96(0.05)* / 0.16(0.02)*
AA / C / 2.73(0.86) / 3.74(0.48) / 0.57(0.05) / 0.11(0.01)
P / 13.24(1.14)* / 17.78(0.83)* / 1.55(0.06)* / 0.14(0.01)*
NP / 13.43(1.91)* / 17.81(1.57)* / 1.61(0.13)* / 0.12(0.01)

Table S5. Three-way MANOVA with plantation type, forest compartment and treatment as independent variables, and nitrogenase activity, BNF rate and standing stock as response variables.

F / Sig.
Plantation type / 87.97 / <0.001
Forest compartment / 59.39 / <0.001
Treatment / 4.67 / <0.001
Plantation type ´ Forest compartment / 10.70 / <0.001
Plantation type ´ Treatment / 1.06 / 0.393
Forest compartment ´ Treatment / 4.29 / <0.001
Plantation type ´ Forest compartment ´ Treatment / 2.48 / 0.013

Plantation type: EU and AA plantations; Forest compartment: bulk soil, forest floor, rhizoshpere; Treatment: C, P addition, and NP addition.

Table S6. Three-way MANOVA with plantation type, forest compartment and treatment as independent variables, and DIN concentration and AP concentration as response variables.

F / Sig.
Plantation type / 17.02 / <0.001
Forest compartment / 15.96 / <0.001
Treatment / 17.88 / <0.001
Plantation type ´ Forest compartment / 3.88 / 0.035
Plantation type ´ Treatment / 2.70 / 0.042
Forest compartment ´ Treatment / 1.38 / 0.254
Plantation type ´ Forest compartment ´ Treatment / 1.63 / 0.182

Plantation type: EU and AA plantations; Forest compartment: bulk soil and rhizoshpere; Treatment: C, P addition, and NP addition.

Table S7. Three-way MANOVA with plantation type, forest compartment and treatment as independent variables, and total N concentration and total P concentration as response variables.

F / Sig.
Plantation type / 245.29 / <0.001
Forest compartment / 922.71 / <0.001
Treatment / 10.06 / <0.001
Plantation type ´ Forest compartment / 184.86 / <0.001
Plantation type ´ Treatment / 4.43 / 0.004
Forest compartment ´ Treatment / 9.83 / <0.001
Plantation type ´ Forest compartment ´ Treatment / 5.34 / 0.001

Plantation type: EU and AA plantations; Forest compartment: root and leaf; Treatment: C, P addition, and NP addition.