Table A: Analysis of variance of biodegradable films Thickness

SourceVariation / DF / SS / MS / F / P-value
Lysosyme / 2 / 0.00123889 / 0.00061944 / 17.15 / 0.0001
pH / 3 / 0.00031944 / 0.00010648 / 2.95 / 0.0531
Lysosyme*pH / 6 / 0.00027222 / 0.00004537 / 1.26 / 0.3139
Error / 24 / 0.00086667 / 0.00003611
Total / 35 / 0.00269722

Table B: Analysis of variance of biodegradable filmsSolubility

SourceVariation / DF / SS / MS / F / P-value
Lysosyme / 2 / 43.07707222 / 21.53853611 / 85.85 / <0.0001
pH / 3 / 44.20562222 / 14.73520741 / 58.74 / <0.0001
Lysosyme*pH / 6 / 14.72252778 / 2.45375463 / 9.78 / <0.0001
Error / 24 / 6.0210000 / 0.2508750
Total / 35 / 108.0262222

Table C: Analysis of variance of biodegradable filmsSolubility of the jackfruit starch biodegradable film (pH 6.0) in acid solution and water, respectively

SourceVariation / DF / SS / MS / F / P-value
Lysosyme / 2 / 26.85254444 / 13.42627222 / 50.62 / <0.0001
solvent / 1 / 10.08005000 / 10.08005000 / 38.01 / <0.0001
Error / 14 / 3.71316667 / 0.26522619
Total / 17 / 40.64576111

Table D: Analysis of variance of biodegradable filmsWater vapor permeability

SourceVariation / DF / SS / MS / F / P-value
Lysosyme / 2 / 9.25E-8 / 4.625E-8 / 13.87 / 0.0008
pH / 3 / 5E-9 / 1.6666667E-9 / 0.50 / 0.6893
Lysosyme*pH / 6 / 2.75E-8 / 4.5833333E-9 / 1.37 / 0.3001
Error / 12 / 4E-8 / 3.3333333E-9
Total / 23 / 1.65E-7

Mechanical properties

Table E: Analysis of variance of biodegradable filmsYoung’s modulus

SourceVariation / DF / SS / MS / F / P-value
Lysosyme / 2 / 56736.60436 / 28368.30218 / 958.27 / <0.0001
pH / 3 / 13640.37268 / 4546.79089 / 153.59 / <0.0001
Lysosyme*pH / 6 / 12556.31614 / 2092.71936 / 70.69 / <0.0001
Error / 12 / 355.24540 / 29.60378
Total / 23 / 83288.53858

Table F: Analysis of variance of biodegradable filmsElongation percent at break

SourceVariation / DF / SS / MS / F / P-value
Lysosyme / 2 / 556.9219750 / 278.4609875 / 259.14 / <0.0001
pH / 3 / 653.3402125 / 217.7800708 / 202.67 / <0.0001
Lysosyme*pH / 6 / 796.0653250 / 132.6775542 / 123.47 / <0.0001
Error / 12 / 12.894850 / 1.074571
Total / 23 / 2019.222363

Table G: Analysis of variance of biodegradable filmsTensile strength

SourceVariation / DF / SS / MS / F / P-value
Lysosyme / 2 / 13.28675833 / 6.64337917 / 209.82 / <0.0001
pH / 3 / 0.96894583 / 0.32298194 / 10.20 / 0.0013
Lysosyme*pH / 6 / 0.58174167 / 0.09695694 / 3.06 / 0.0468
Error / 12 / 0.37995000 / 0.03166250
Total / 23 / 15.21739583