Curriculum Vitae

Bent Jakobsen

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Curriculum Vitae

Bent Jakobsen, Ph.D

Current: Chief Scientific Officer

Avidex Ltd

57c Milton Park

Abingdon

Oxfordshire OX14 4RX

1993 – 2000: University Research Lecturer /

Head of the T Cell Receptor Group

Nuffield Department of Clinical Medicine/MRC

Molecular Immunology Group

University of Oxford

Institute of Molecular Medicine

John Radcliffe Hospital

Headington

Oxford OX3 9DS

England

1990 – 1993: Senior Research Fellow of the

Danish Natural Research Science Council

Department of Molecular Biology

University of Aarhus

C.F. MØllers Alle’, Building 130

8000 Aarhus C

Denmark

Projects: Transcriptional regulation by Heat Shock Factor and studies of domain structure in Topoisomerase II

1987 – 1990: PhD at the Laboratory of Molecular Biology

Medical Research Council

Hills Road

Cambridge CB2 2QH

England

Research Fellow of the Danish Science Research Councils (1987 – 1989)

Research Fellow of the Medical Research Council (1990)

Research Project: Cloning and characterisation of Heat Shock Factor from Yeast.

Supervisor: Hugh R. B. Pelham, FRS.

Selected publications in immunology

1.  Reid, S.W., McAdam, S., Smith, K.J., Klenerman, P., O’Callaghan, C.A., Harlos, K., Jakobsen, B.K., McMichael, A.J., Bell, J.I., Stuart, D.I., Jones, E.Y. (1996) “Antagonist HIV-1 Gag Peptides Induce Structural Changes in HLA B8” J. Exp. Med. 184 p2279-2286.

2.  Vessey, S.J.R., Barouch, D.H., McAdam, S.N., Tussey, L.G., Davenport, M.A., O’Callaghan, C.A., Bell, J.I., McMichael, A.J., Jakobsen, B.K. (1997) “Engagement of a T cell Receptor by Major Histocompatibility Complex Irrespective of Peptide” Eur. J. Immmunol 27 p879-885.

3.  Gao, G.F., Tormo, J., Gerth, U.C., Wyer, J.R., McMichael, A.J., Stuart, D.I., Bell, J.I., Jones, E.Y., Jakobsen, B.K. (1997) “Crystal structure of the complex between human CD8aa and HLA-A2” Nature 387 p630-634.

4.  O’Callaghan, C.A., Tormo, J., Willcox, B.E., Braud, V.M., Jakobsen, B.K., Stuart, D.I., McMichael, A.J., Bell, J.I., Jones, E.Y. (1998) “Structural Features Impose Tight Peptide Binding Specificity in the Nonclassical MHC Molecule HLA-E” Molecular Cell 1 p531-541.

5.  Purbhoo, M.A., Sewell, A.K., Klenerman, P., Goulder, P.J.R., Hilyard, K.L., Bell, J.I., Jakobsen, B.K., Phillips, R.E. (1998) “Copresentation of Natural HIV-1 Agonist and Antagonist Ligands Fails to Induce the T cell Receptor Signaling Cascade” Immunology 95 p4527-4532.

6.  Gao, G.F., Gerth, U.C., Wyer, J.R., Willcox, B.E., O’Callaghan, C.A., Zhang, Z., Jones, E.Y., Bell, J.I., Jakobsen, B.K. (1998) “Assembly and Crystallization of the Complex Between the Human T Cell Coreceptor CD8a Homodimer and HLA-A2” Protein Science 7 p1245-1249.

7.  Wyer, J.R., Willcox, B.E., Gao, G.F., Gerth, U.C., Davis, S.J., Bell, J.I., Anton van der Merwe, P., Jakobsen, B.K. (1999) T cell receptor and coreceptor CD8aa bind peptide-MHC independently and with distinct kinetics. Immunity 10 p219-225.

8.  Willcox, B.E., Gao, G.F., Wyer, J.R., Ladbury, J.E., Bell, J.I., Jakobsen, B.K., Anton van der Merwe, P. (1999) TCR Binding to Peptide-MHC Stabilizes a Flexible Recognition Interface. Immunity Vol. 10 p357-365.

9.  Sewell, A.K., Gerth, U.C., Price, D.A., Purbhoo, M.A., Boulter, J.M., Gao, G.F., Bell, J.I., Phillips, R.E., Jakobsen, B.K. (1999) Antagonism of Cytotoxic T-lymphocyte Activation by Soluble CD8. Nature Medicine Vol. 5, No. 4, p399-404.

10.  Willcox, B.E., Gao, G.F., Wyer, J.R., O’Callaghan, C.A., Boulter, J.M., Jones, E.Y., Anton van der Merwe, P., Bell, J.I., Jakobsen, B.K. (1999) Production of Soluble ab T-cell Receptor Heterodimers Suitable for Biophysical Analysis of Ligand Binding. Protein Science 8 p2418-2423.

11.  Gao, G.F., Willcox, B.E., Wyer, J.R., Boulter, J.M., O’Callaghan, C.A., Maenaka, K., Stuart, D.I., Jones, E.Y., Anton van der Merwe, P., Bell, J.I., Jakobsen, B.K. (2000) Classical and Nonclassical Class I Major Histocompatibility Complex Molecules Exhibit Subtle Conformational Differences that Affect Binding to CD8aa. The Journal of Biological Chemistry Vol. 275, No. 20, p15232-15238.

12.  Gao G.F., Jakobsen, B.K., (2000) Molecular Interactions of Coreceptor CD8 and MHC Class I: The Molecular Basis for Functional Coordination with the T-Cell Receptor. Immunology Today Vol 21, No. 12, pp597-646 [246] issn: 0167-5699 p630-636.

13.  Purbhoo, M.A., Boulter, J.M., Price, D.A., Vuidepot, A-L., Hourigan, C.S., Dunbar, P.R., Olson, K., Dawson, S.J., Phillips, R.E., Jakobsen, B.K., Bell, J.I., Sewell, A.K. (2001) The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor z Chain. The Journal of Biological Chemistry Vol. 276 No. 35, p32786-32792.

14.  Li, Y., Moysey, R., Molloy, P.E., Vuidepot, A-L., Mahon, T., Baston, E., Dunn, S., Liddy, N., Jacob, J., Jakobsen, B.K., Boulter, J.M. (2005) Directed evolution of human T-cell receptors with picomolar affinity by phage display. Nature Biotechnology 23, 349-354.

15.  Laugel, B., Boulter, J.M., Lissin, N., Vuidepot, A-L., Li, Y., Gostick, E., Crotty, L.E., Douek, D.C., Hemelaar, J., Price, D.A., Jakobsen, B.K., Sewell, A.K. (2005) Design of soluble recombinant T cell receptors for antigen targeting and T cell inhibition. The Journal of Biological Chemistry Vol 280, No. 3, p1882-1892.