Table 1, supplementary information
Proteins identified by MS/MS data and database search (MSDG, NCBI-nr)
accession,species / protein name / unique peptides / sequence coverage / protein score* / best
peptide score** / pI
AAP45313
Papaver somniferum / SAM: 3´-hydroxy N-methylcoclaurine 4`-O-methyltransferase / 11)
4 EST1) / 16.9 % / 65 / 45 / 5.1
NP_001053139
Oryza sativa / glyceraldehyde 3-phosphate dehydrogenase C / 4 / 17.6 % / 121 / 47 / 5.6
NP_001063993
Oryza sativa / peptidylprolyl-cis/trans-isomerase (cyp1 rice) / 3 / 20.6 % / 37 / 52 / 73
NP_001055754
Oryza sativa / Heat shock protein HSP70 / 2 / 3.7 % / 67 / 46 / 5.5
* protein score: score of all fragment peptides aligned by the Mascot search in the MSDB library;
** best peptide score: highest score of a single peptide by searching with BLASTp in the NCBI library nr.
1) The sequence LPLSNVNSDNLYR was found in both libraries (score:65) and matched to the annotated protein of Papaver. High homology exists to enzymes of benzylisoquinoline biosynthesis in Coptis japonica, Coptis chinensis and Thalictrum. Four DNA sequences were identified in the Eschscholzia EST libraries, which after translation all matched the reported Papaver protein.
Table 2, supplementary information
Pimers used to identify the mRNA of the selected proteins (cf. Table 1) via RT-PCR
protein / primer sequence 5'-3'forward (sense) / primer sequence 5'-3'
reverse (antisense)
4' OMT / agt cct tcg ttc tgc agt tga g / tca ctt ctc tcc ctt cca cc
GAPDH-C / atg ggt tca gga aag a / ttg gca aga gtg aac g
HSP 70 / agt gat gcc act gtt caa gc / aac aca cca ggt tgg ttg tc
PPIase / aat gtc gaa ccc gaa ggt ct / gtg aaa tca cca cct tgg ca