Plate-1 Colony morphology of B. thuringiensis isolates and B. thuringiensis subsp kurstaki HD-1.

Plate-2. Single colony purified Bacillus colonies on Nutrient agar

Plate-3. Selection of Bacillus colonies with chalky white colour and granular border

Plate- 4 . Photomicrographs of cell and spore and crystal morphology of various

Bacillus thuringiensis isolates obtained from western ghats of karnataka

Vegetative cells (12 hours)

(3800 x)

Sporulated cells (24 hours)

(3800 x)

Lysed culture (72 hours)

(3800 x)

Crystal morphology

Bipyramidal

(3800 x)

Rhomboidal

(3800 x)

Spherical

(3800 x)

Table 4. Crystal protein and gene features of the native B. thuringiensis isolates.

Isolates / cry gene profiles / Protein pattern a / Crystal morphology b / Spore position
JK PDR4 / cry26, / 95, 60, 43, 26 / BP, CB, RT / Terminal
JK PDR1 / cry8B, cry5A, cry5B / 130, 95, 60, 40, 30, 17 / BP, RT, SP / Sub terminal
JK SHL1 / cry8A / 130, 60, 43, 26 / R, BP / Sub terminal
JK PDR2 / cry7A, cry7Ab / 60, 50, 40, 30,17 / BP, R / Sub terminal
JK SHL3 / cry1I, cry3A / 55,43,26 / IR, BP,SP / Terminal
JK B SHL2 / cry1B / 50,40,26,17 / SP, BP / Terminal
Bt RJ01 / cry1, cry1I / 120, 55, 43 / BP, CB / Sub terminal
Bt RJ02 / cry1, cry1I, cry1F / 60,40,15 / BP, CB, RT / Terminal
Bt RJ03 / cry1I, cry4A / 130,95,55,43,30 / BP, RT, SP / Terminal
Bt RJ04 / cry1 cry1I / 130,95, 55,43,30 / BP, RT, SP / Sub terminal
Bt RJ06 / cry1I, cry1F / 170,55,43 / IR, BP,SP / Terminal
Bt RJ12 / cry1I, cry1Ab, cry4A / 130,95,60,50,43 / EL, SP / Terminal
Bt RJ13 / cry1I / 130,95,60,50,43 / BP, RT, SP / Terminal
Bt RJ14 / cry1I / 55,40 / BP, SP / Terminal
Bt RJBHL / cry9D,cry9 , cry6A, cry5A / 130,95,60,50,43, 26, 20,17,12 / BP, RT, SP / Sub terminal
Bt RJARJ / cry9D, cry9 / 55, 43, 30 / BP, CB, RT / Sub terminal
Bt RJKHR / cry9D, cry6A / 95,72,60,55,43,30,26,15 / IR, BP,SP / Sub terminal
Bt RJCHA / cry9D, cry9 / 55, 43, 30 / BP, R / Sub terminal
Bt RJ07 / cry26, cry8A, cry8Ka / 120, 80, 60 / BP, CB, RT / Sub terminal
Bt RJ08 / cry26 / 120, 60,70 / BP, SP / Sub terminal
Bt RJ09 / cry9,cry26,cry28 / 135, 60, 40, 52 / BP, RT, SP / Sub terminal
Bt RJ10 / cry9,cry26, cryIIIC / 135, 60 / BP, CB, RT / Sub terminal
Bt RJ11 / cry26 / 135, 60 / RT, SP / Sub terminal
Bt RJ12 / cry26, cryIIIC / 120, 80, 60 / BP, R / Sub terminal
Bt BOM1 / cry1I, cry4A / 130,60 / EL, SP / Sub terminal
Bt KZR3 / cry1I / 130,60,35 / BP, RT, SP / Terminal
Bt SIK01 / cry1I, cry1Ab / 120, 66,50 / SP, SL, IR, / Terminal
Bt SIK02 / cry1I / 120, 66,50 / RT, SP / Terminal
Bt SIK03 / cry1I / 120, 66,50,30 / BP, SP / Terminal
Bt SIK04 / cry1I, cry3A, cry1F / 120, 66,50 / BP, CB, RT / Sub terminal
Bt SIK05 / cry1I, cry4A / 120, 66,50,30 / BP, RT, SP / Terminal
Bt SIK06 / cry1I / 50,40,20 / SP, SL, IR, / Terminal
Bt SIK07 / cry1I, cry1Ab / 120,56,40, / BP, R / Sub terminal
Bt SIK08
Bt SIK09 / cry1I
cry1I, cry3A,cry1F / 135, 60
135, 60 / BP, SP
BP, RT, SP / Sub terminal
Terminal

a Major protein bands on SDS–PAGE gels.

b SP: Spherical, RT: Rectangular, IR: Irregular, BP: Bipyramidal, SL: Spindle, C: Cuboidal, R: Rhomboidal.