Supplementary Table 1. Cosegregation of the susceptibility with the transgene Xa13 in four T1 families
Rice material / Average lesion lengtha / Xa13bParental lines
IRBB13 (xa13)c / 2.10 0.76d / –
1.98 0.75e / –
IR24 (Xa13)c / 12.05 1.01d / +
13.12 3.42e / +
Transgenic family D09O3d
1 / 14.70 1.73 / +
2 / 11.23 1.89 / +
3 / 10.37 1.69 / +
4 / 9.40 1.30 / +
5 / 3.13 0.35 / –
6 / 10.53 2.27 / +
7 / 9.77 1.01 / +
8 / 12.27 1.70 / +
9 / 14.00 4.50 / +
10 / 11.40 1.13 / +
11 / 10.73 2.06 / +
12 / 14.75 3.46 / +
13 / 2.13 0.74 / –
14 / 2.20 0.87 / –
Transgenic family D09O6d
1 / 2.27 0.15 / –
2 / 2.53 0.15 / –
3 / 2.27 0.15 / –
4 / 1.77 0.67 / –
5 / 10.07 4.37 / +
6 / 12.43 1.75 / +
7 / 14.23 0.67 / +
8 / 15.23 1.46 / +
9 / 13.83 3.21 / +
10 / 11.83 3.74 / +
11 / 1.67 0.21 / –
12 / 10.50 0.70 / +
13 / 15.07 1.47 / +
14 / 14.83 4.46 / +
15 / 14.30 0.40 / +
16 / 15.40 1.81 / +
17 / 10.90 3.12 / +
18 / 2.70 0.75 / –
19 / 15.73 1.69 / +
Transgenic family D09O19d
1 / 12.50 0.71 / +
2 / 2.27 0.47 / –
3 / 11.00 1.11 / +
4 / 13.90 5.40 / +
5 / 9.70 0.56 / +
6 / 9.67 0.32 / +
7 / 10.43 0.86 / +
8 / 1.80 0.26 / –
9 / 12.57 4.11 / +
10 / 9.00 0.85 / +
11 / 12.13 2.18 / +
12 / 10.6 0.56 / +
13 / 1.70 0.30 / –
14 / 9.90 2.46 / +
15 / 12.07 2.80 / +
Transgenic family D09O7e
1 / 11.23 0.55 / +
2 / 9.37 2.80 / +
3 / 8.00 2.31 / +
4 / 10.13 4.29 / +
5 / 1.03 0.40 / –
6 / 8.27 1.72 / +
7 / 11.17 2.15 / +
8 / 12.90 2.43 / +
9 / 0.63 0.25 / –
10 / 11.00 0.14 / +
11 / 1.87 0.90 / –
12 / 1.43 0.75 / –
13 / 0.90 0.36 / –
14 / 1.60 0.57 / –
15 / 0.63 0.32 / –
16 / 1.10 0.36 / –
17 / 1.43 0.59 / –
18 / 12.80 1.77 / +
19 / 14.67 6.31 / +
20 / 20.80 3.37 / +
21 / 9.70 2.12 / +
22 / 8.10 3.22 / +
23 / 7.57 2.67 / +
24 / 10.90 1.93 / +
25 / 0.70 0.55 / –
26 / 10.37 3.84 / +
27 / 6.47 1.86 / +
28 / 10.57 0.86 / +
29 / 12.00 4.95 / +
30 / 1.47 0.47 / –
31 / 9.47 3.54 / +
32 / 9.75 1.06 / +
33 / 11.70 4.86 / +
34 / 14.23 3.55 / +
35 / 0.60 0.14 / –
36 / 9.95 1.20 / +
37 / 7.95 0.21 / +
38 / 11.10 0.42 / +
39 / 11.30 2.14 / +
40 / 10.95 1.06 / +
aThree to five uppermost fully expanded leaves of each plant were inoculated with Xoo strain PXO99 using the leaf-clipping method. Lesion length was measured at 14 days after inoculation. Standard deviation for each sample was calculated.
bXa13-specific band determined by PCR amplification using primers X13-seq2F and X13-seq2R (Supplementary Table S4).
cIRBB13 and IR24 are near-isogenic lines. IRBB13 is the recipient of transgene Xa13. The sequences of Xa13 and its promoter region (approximate 1.4 kb) in rice lines IR24 and IR64 are identical.
dThe parental lines and T1 families derived from T0 lines D09O3, D09O6 and D09O19 were inoculated at maximum tillering stage.
eThe parental lines and T1 family derived from T0 line D09O7 were inoculated at seedling (5-week old) stage.
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