Molecular characterization of HetRV8-ir1, a partitivirus of the invasive conifer pathogenic fungus H. irregulare.Eeva J. Vainio, Paolo Capretti, Emma Motta and Jarkko Hantula. E-mail: (Finnish Forest Research Institute)

Table S1.Sequence similarities between HetRV8-ir1 and related viruses

Virus / RdRP / CP
Accession / BlastP similarity/query coverage / BlastN similarity /query coverage / Accession / BlastP similarity /query coverage / BlastN similarity /query coverage
Grapevine associated partitivirus-1 / ADO60922 / 68/99 / 70/77 / naa / na / na
Fusarium poae virus 1 / NP_624349 / 70/94 / 71/82 / NP_624348 / 60/99 / 73/64
Pleurotus ostreatus virus 1 / YP_227355 / 59/98 / 78/75 / YP_227354 / 78/100 / 75/95
Helicobasidium mompa partitivirus V1-1 / BAD32677 / 49/98 / -b / na / na / na
Primula malacoides virus China/Mar2007 / YP_003104768 / 46/98 / - / YP_003104769 / 23/94 / ndc
Rosellinia necatrix partitivirus 1-W8 / YP_392480 / 46/99 / - / YP_392481 / 23/48 / na
Rosellinia necatrix partitivirus 4 / BAM36402 / 47/98 / 70/45 / na / na / na
Rosellinia necatrix partitivirus 3 / BAM36401 / 45/98 / - / na / na / na
Rhizoctonia solani virus 717 / NP_620659 / 46/98 / 65/40 / NP_620660 / 24/49 / nd
Cannabis cryptic virus / AET80948 / 45/98 / - / AET80949 / 23/96 / nd
Atkinsonella hypoxylon virus / NP_604475 / 39/82 / - / NP_604476 / 20/92 / nd
Ceratocystis polonica partitivirus / AAP86639 / 38/81 / - / AAP86640 / 25/88 / nd
Ceratocystis resinifera virus 1 / YP_001936016 / 38/81 / - / YP_001936015 / 25/88 / nd
Heterobasidion RNA virus 7 / AEX87907 / 34/86 / - / AEX87908 / 24/65 / nd
Heterobasidion annosum P-type partitivirus / AAL79540 / 33/88 / - / na / na / na
Heterobasidion RNA virus 2 / ADL66905 / 32/87 / - / ADL66906 / 21/78 / nd
Heterobasidion RNA virus 4 / ADV15443 / 28/44 / - / na / na / na
Heterobasidion annosum virus / AAK52739 / 27/44 / - / na / na / na
Heterobasidion RNA virus 5 / ADV15444 / 24/75 / - / na / na / na
Heterobasidion RNA Virus 3 / ACO37245 / 29/41 / - / nd / nd / nd
Heterobasidion RNA virus 1 / ADV15446 / 27/44 / - / nd / nd / nd
Heterobasidion parviporum partitivirus Fr 110B / CBJ23785 / 30/30 / - / na / na / na

aNo sequence available (na)

b Sequences with less than 25% query coverage are not shown (-)

cNot detected by BlastP/BlastN due to low similarity (nd)

Fig. S1 Bayesian analysis of partitivirus CP sequences conducted with the gamma among-site rate variation and 1.1 × 106 cycles for the MCMC algorithm, sampling 1 tree per 200 cycles, and discarding 105 samples as burn-in. The scale bar indicates 0.4 amino acid substitutions per site, and numbers at branch nodes show percentage posterior probabilities of over 90%. The amino acid sequence alignment was generated using MAFFT version 6.717b. Virus name abbreviations are as follows: AhV (Atkinsonella hypoxylon partitivirus); CCV (Cannabis cryptic virus); CpV (Ceratocystis polonica partitivirus); CrV (Ceratocystis resinifera partitivirus); FpV-1 (Fusarium poae virus 1); HetRV2-pa1 (Heterobasidion RNA virus 2); HetRV7-pa1 (Heterobasidion RNA virus 7); PoV-1 (Pleurotus ostreatus virus 1); PmV1 (Primula malacoides virus China/Mar2007); RhsV-717 (Rhizoctonia solani virus 717); RnV1-W8 (Rosellinia necatrix partitivirus 1-W8).The sequence accession codes are indicated in Table S1