Supplemental Table 1. Taxon Names, Mczbase Codes, Original MCZ DNA Database Codes, Genbank
Supplemental Table 1.Taxon names, MCZbase codes, original MCZ DNA database codes, GenBank codes, and collection locality information for all specimens used.
Species / MCZbase code (Invert. Zoo.) / Old MCZ DNA code (DNA-) / Population code / H3 / 18S / 28S / COI / 12S / 16S / Location / Lat., Long.Metasiroamericanus / 134557 / 101532 / A / KJ405955–964 / KT200610-9 / KT200839-48 / Torreya State Park, Liberty Co., Florida, USA / 30.56472, -84.95139
“ / 133791, 133792 , 133797 / 103805, 103806, 103811 / B / KT201076 / KT201064 / KT201067 / KJ405965–76, KJ405986–992 / KT200620-31, KT200641-7 / KT200849-60, KT200870-6 / Torreya State Park, Liberty Co., Florida, USA / 30.568, -84.95167
“ / 133793–5 / 103807–9 / C / KJ405977–85 / KT200632-40 / KT200861-9 / Upper Sweetwater Creek, Liberty Co., Florida, USA / 30.52558, -84.93677
“ / 134493 / 103813 / D / KJ405993–999 / KT200648-54 / KT200877-83 / Flat Creek, Gadsden Co., Florida, USA / 30.62793, -84.83528
“ / 134492 / 103814 / E / KJ406000–009 / KT200655-64 / KT200884-93 / Chattahoochee, Gadsden Co., Florida, USA / 30.70232, -84.82556
“ / 133808 / 105654 / P / KT201078 / KT201061 / KT201068 / KJ406107–26 / KT200762-80 / KT200991-1010 / Florida Caverns State Park, Jackson Co., Florida, USA / 30.82119, -85.24639
“ / 133809 / 105655 / Q / KJ406127–36 / KT200782-91 / KT201011-20 / “ / “
“ / 133810 / 105656 / R / KJ406137–43 / KT200792-8 / KT201021-27 / “ / “
“ / 133811 / 105657 / S / KT201073 / KJ406144–53 / KT200799-808 / KT201028-37 / “ / “
“ / 133812 / 105662 / T / KT201074 / KJ406154–163 / KT200809-18 / KT201038-47 / “ / 30.81433, -85.24667
“ / 133813 / 105663 / U / KT201079 / KT201060 / KJ406164–175 / KT200819-30 / KT201048-60 / “ / 30.81408, -85.24722
M. sassafrasensis / 134534–5 / 105643–4 / F / KT201075, KT201080 / KT201063 / KT201070 / KJ406010–21 / KT200665-76 / KT200894-905 / Jocosse Gorge, Sassafras Mt., Pickens Co., South Carolina, USA / 35.06228, -82.795
M. savannahensis / 133799 / 105645 / G / KJ406022–30 / KT200677-85 / KT200906-14 / Kingfisher Pond, Savannah National Wildlife Refuge, Jasper Co., South Carolina, USA / 32.18923, -81.08
“ / 133800 / 105646 / H / KT201077 / KT201062 / KT201069 / KJ406031–40 / KT200686-95 / KT200915-24 / “ / “
“ / 133801 / 105647 / I / KT201066 / KJ406041–9 / KT200696-704 / KT200925-33 / “ / “
“ / 133802 / 105648 / J / KT201071 / KJ406050–8 / KT200705-13 / KT200934-42 / “ / “
“ / 133803 / 105649 / K / KT201065 / KJ406059–67 / KT200714-22 / KT200943-51 / “ / “
“ / 133804 / 105650 / L / KT201072 / KJ406068–77 / KT200723-32 / KT200952-61 / “ / “
“ / 133805 / 105651 / M / KJ406078–87 / KT200733-42 / KT200962-71 / “ / “
“ / 133806 / 105652 / N / KJ406088–96 / KT200743-51 / KT200972-80 / “ / “
“ / 133807 / 105653 / O / KT201081 / KJ406097–106 / KT200752-61 / KT200981-90 / “ / “
Aorakidenticulata major / 134644 / 100959 / EU673700 / EU673620 / EU673656 / DQ992203 / EU673585 / New Zealand / -43.03408, 171.76464
Huitacaboyacaensis / 132276 / 101407 / DQ518167 / DQ518090 / DQ825596 / DQ518129 / DQ518050 / Colombia / 5.77956, -73.45377
Metagovea sp. / 132282 / 101409 / JF786426 / DQ825544 / DQ825599 / DQ825646 / DQ825619 / Colombia / 1.28500, -78.07367
Metagovea sp. / 132283 / 101410 / DQ518168 / DQ518091 / DQ518048 / GQ912860 / JF935034 / Colombia / 1.28500, -78.07367
Ogoveacameroonensis / 132315 / 104617 / JF786417 / JF934960 / JF934994 / JF786392 / JF935026 / Cameroon / 3.64621, 11.29079
Parogovia sp. / 132305 / 101052 / DQ825515 / DQ825546 / DQ825601 / DQ825648 / DQ825620 / Equatorial Guinea / 1.65815, 10.31143
Parogoviasironoides / 132302 / 101059 / DQ518169 / DQ518092 / DQ825606 / DQ518131 / DQ518051 / Bioko, Equatorial Guinea / 3.72570, 8.83828
Parogoviasironoides / 132304 / 101061 / DQ825519 / DQ825550 / DQ825607 / DQ825650 / DQ825624 / Bioko, Equatorial Guinea / 3.70284, 8.87520
Parogovia sp. / 132299 / 104615 / JF786427 / JF934966 / JF935021 / JF786410 / KT200831 / JF935044 / Cameroon / 4.80084, 9.66326
Parogovia cf. sironoides / 132293 / 104619 / JF786428 / JF934967 / JF935022 / JF786409 / KT200832 / JF935045 / Cameroon / 2.74108, 9.88181
Parogoviagabonica / 132297 / 104620 / JF934969 / JF935019 / JF786411 / KT200833 / JF935047 / Gabon / 0.50448, 12.79525
Pettalusthwaitesi / 78875 / 101227 / DQ518138 / DQ517975 / DQ518018 / DQ518101 / DQ518057 / Sri Lanka / 7.27251, 80.59333
Siro rubens / 132391 / 100457 / AY428834 / AY428818 / DQ133735 / DQ513111 / France / 44.08338, 3.58140
Leptopsalis sp. / 70646 / 101934 / GQ488131 / GQ488296 / GQ488358 / GQ488165 / KT200834 / GQ488208 / Sulawesi, Indonesia / 0.759167, 124.421667
Miopsalis sp. / 134360 / 103259 / GQ488116 / GQ488260 / GQ488375 / GQ488142 / KT200835 / GQ488193 / Borneo, Malaysia / 5.81, 116.24
Suzukielussauteri / 132256 / 101543 / DQ518166 / DQ513138 / DQ513116 / DQ513108 / DQ518086 / Japan / 35.63440, 139.24122
Troglosiroaelleni / 134764 / 100345 / DQ518164 / AY639497 / AY639525 / AY639584 / AY639555 / New Caledonia / -21.1833, 165.3059
Troglosirolongifossa / 134765 / 100867 / DQ518165 / DQ518089 / DQ825582 / DQ518127 / DQ518084 / New Caledonia / -22.3527, 166.9736
Troglosiromonteithi / 51948 / 101580 / EU887101 / EU887116 / EU887043 / KT200836 / EU887074 / New Caledonia / -21.6000, 165.7167
Troglosiro cf. juberthiei / 133875 / 101703 / EU887045 / KT200837 / EU887082 / New Caledonia / -22.25, 166.816667
Troglosirourbanus / 134780 / 101710 / JF786340 / EU887102 / EU887119 / EU887040 / EU887073 / New Caledonia / -22.1945, 166.5017
Troglosirowilsoni / 134787 / 102324 / EU887107 / EU887125 / EU887061 / KT200838 / EU887075 / New Caledonia / -22.1770, 166.5106
Forsteropsalisturneri / 134832 / 100783 / KJ871492 / KJ871558 / New Zealand / -46.01975, 167.74095
Supplemental Table 2.Primers and temperature profiles used in this study. All primers are written 5’–3’.
Primer name / Reference / Sequence (5’ to 3’) / Protocol18SrRNA
/ Standard1, T=471F / (Giribet et al. 1996) / TAC CTG GTT GAT CCT GCC AGT AG
3F / “ / GTT CGA TTC CGG AGA GGG A
4F / “ / CCA GCA GCC GCG CTA ATT C
4R / “ / GAA TTA CCG CGG CTG CTG G
5R / “ / CTT GGC AAA TGC TTT CGC
9R / “ / GAT CCT TCC GCA GGT TCA CCT AC
18Sa2.0 / (Whiting et al. 1997) / ATG GTT GCA AAG CTG AAA C
18Sbi / “ / GAG TCT CGT TCG TTA TCG GA
28SrRNA
/ Standard1, T=4728SD1f / (Park and Foighil 2000) / GGG ACT ACC CCC TGA ATT TAA GCA T
28Sa / (Whiting et al. 1997) / GAC CCG TCT TGA AAC ACG GA
28Sb / “ / TCG GAA GGA ACC AGC TAC
28SrD4b / (Whiting 2002) / CCT TGG TCC GTG TTT CAA GAC
28SrD5b / “ / CCA CAG CGC CAG TTC TGC TTA C
28Srd4.8a / (Schwendinger and Giribet 2005) / ACC TAT TCT CAA ACT TTA AAT GG
28Srd7b1 / “ / GAC TTC CCT TAC CTA CAT
16SrRNA / Standard1, T=46
16Sa / (Xiong and Kocher 1991) / CGC CTG TTT ATC AAA AAC AT
16SaPo / CGA CTG TTT ATC AAA AAC AT
16Sb / (Xiong and Kocher 1991) / CTC CGG TTT GAA CTC AGA TCA
16SbPo / CAC CGG TTT GAA CTC AAA TCA
Front loop of 12S / Standard1, T=46
12SFb1 / CGT GCC AGC CGC CGC GGT TAG AC
12SFb1-long / CGT GCC AGC CGC CGC GGT TAG ACC A
12SaiREV / ATA ATA GGG TAT CTA ATC CTA GTT T
12SaiREV3 / ATA ATA GGG TAT CTA ATC CTA GTT TAT C
12S / Standard1, T=46
12Sai / (O'Neill and Dobson 2008) / AAA CTA GGA TTA GAT ACC CTA TTA T
12SdegR / VAR AGY GAC GGG CRA TAT GTA CAT
COI / Standard1, T=46
LCO1490 / (Folmer et al. 1994) / GGT CAA CAA ATC ATA AAG ATA TTG G
LCOdeg1 / TCH ACD AAY CAY AAA GAT ATT GGW ACW AT
LCOMeta2 / KKT CAA CGA ATC ATA AAG ATA TTG G
LCOMeta8 / TKK TCA ACG AAT CAT AAA GAT ATT
HCOoutout / (Whiting 2002) / GTA AAT ATA TGR TGD GCT C
HCOooOpi2 / GTA AAT ATA TGR TGD GCT CAT AC
HCOoutMeta / ATA GTG ACA ATA AAA TTA ATA GCT CC
Histone H3 / TD62, TD6-483
H3aF / (Xiong and Kocher 1991) / ATG GCT CGT ACC AAG CAG ACV GC
H3aR / “ / ATA TCC TTR GGC ATR ATR GTG AC
1 Standard: 35x[94°C (0:30), T (0:30), 72°C (1:30)], [72°C (10:00)]
2 TD6: [94°C (1:00), 58–45°C (1:00), 72°C (1:00)], 25x[94°C (0:30), 58°C (0:45), 72°C (0:45)] , [72°C (3:00)]
3 TD6-48 [94°C (1:00), 58.5–48°C (1:00), 72°C (1:00)], 25x[94°C (0:30), 58°C (0:45), 72°C (0:45)] , [72°C (3:00)]
4 TD53: [94°C (1:00), 63–53°C (1:00), 72°C (1:00)], 4x[94°C (1:00), 53°C (1:00), 72°C (1:00)], 25x[94°C (0:30), 58°C (0:45), 72°C (0:45)] , [72°C (3:00)]
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