Online Supplementary Content for ‘Effects of two locust control methods on wood-eating termites in arid Australia’

Table A: Analysis of the effects of fipronil or Metarhizium spray (sprayed vs unsprayed arrays within sites) and time (pre or post treatment, n = 108 per time) on percent wood-eating termite activity at baits using a REML random effect model with site designated as a random factor.

Treatment / factor / Wood eating termites
degrees of freedom
numerator denominator F Value P value / All termites
degrees of freedom
numerator denominator F Value P value
fipronil / sprayed vs unsprayed / 1 / 4 / 0.76 / 0.43 / 1 / 4 / 0.53 / 0.51
time / 1 / 60 / 1.19 / 0.28 / 1 / 60 / 0.75 / 0.39
depth / 1 / 60 / 3.42 / 0.07 / 1 / 60 / 3.69 / 0.06
time X sprayed / 1 / 60 / 0.06 / 0.81 / 1 / 60 / 0.08 / 0.78
depth X sprayed / 1 / 60 / 1.64 / 0.21 / 1 / 60 / 1.10 / 0.30
depth X time / 1 / 60 / 0.27 / 0.61 / 1 / 60 / 0.02 / 0.89
depth X sprayed X time / 1 / 60 / 0.28 / 0.60 / 1 / 60 / 0.56 / 0.46
Metarhizium / sprayed vs unsprayed / 1 / 4 / 0.53 / 0.51 / 1 / 4 / 0.61 / 0.48
time / 1 / 60 / 0.84 / 0.36 / 1 / 60 / 0.63 / 0.43
depth / 1 / 60 / 1.73 / 0.19 / 1 / 60 / 1.12 / 0.30
time X sprayed / 1 / 60 / 0.62 / 0.43 / 1 / 60 / 0.45 / 0.51
depth X sprayed / 1 / 60 / 0.56 / 0.46 / 1 / 60 / 0.51 / 0.48
depth X time / 1 / 60 / 0.23 / 0.64 / 1 / 60 / 0.47 / 0.50
depth X sprayed X time / 1 / 60 / 0.06 / 0.81 / 1 / 60 / 0.16 / 0.69

Table B: Analysis of the effects of fipronil or Metarhizium spray (sprayed vs unsprayed arrays within sites),time (pre or post treatment) and bait depth (above or below-ground) on percent termite baits mass loss at all baits using REML random effects models.

factor / degrees of freedom
numerator denominator / F value / P value
Within fipronil sites (n = 144)
spray vs no spray / 1 / 4 / 0.35 / 0.58
time / 1 / 4 / 0.001 / 0.97
depth / 1 / 4 / 1.66 / 0.27
time X spray / 1 / 4 / 0.06 / 0.82
time X depth / 1 / 4 / 0.07 / 0.80
depth X spray / 1 / 4 / 0.29 / 0.62
depth X spray X time / 1 / 4 / 0.38 / 0.57
Within Metarhizium sites (n = 144)
spray vs no spray / 1 / 4 / 0.57 / 0.49
time / 1 / 4 / 0.01 / 0.94
depth / 1 / 4 / 1.68 / 0.27
time X spray / 1 / 4 / 0.03 / 0.88
time X depth / 1 / 4 / 0.52 / 0.51
depth X spray / 1 / 4 / 0.27 / 0.63
depth X spray X time / 1 / 4 / 0.01 / 0.93

Table C: Analysis of the effects of treatment (control, fipronil and Metarhizium), time (pre or post treatment) and bait depth (above or below-ground) on termite baits mass loss only at baits where termite activity was recorded (n=42 per treatment) using a REML random effects model.

factor / degrees of freedom
numerator denominator / F value / P value
treatment / 2 / 6.0 / 0.85 / 0.47
time / 1 / 6.0 / 0.14 / 0.72
depth / 1 / 6.1 / 5.20 / 0.06
time X treatment / 2 / 6.0 / 0.05 / 0.95
depth X time / 1 / 5.8 / 0.08 / 0.79
depth X treatment / 2 / 6.1 / 0.36 / 0.71
time X treatment X depth / 2 / 5.8 / 0.54 / 0.61

Table D: Analysis of the effects of fipronil or Metarhizium spray (sprayed vs unsprayed arrays within sites) and time (pre or post treatment) on termite community composition (presence of morphospecies at arrays, n=18 per treatment) using PERMANOVA.

factor / degrees of freedom
numerator denominator / Pseudo-F value / P value
fipronil
spray vs no spray / 1 / 35 / 0.53 / 0.79
time / 1 / 35 / 1.86 / 0.14
site (treatment) / 4 / 35 / 1.47 / 0.13
time X spray / 1 / 35 / 1.30 / 0.30
site (treatment) X time / 4 / 35 / 0.52 / 0.89
Metarhizium
spray vs no spray / 1 / 35 / 0.68 / 0.61
time / 1 / 35 / 0.49 / 0.74
site (treatment) / 4 / 35 / 1.73 / 0.06
time X spray / 1 / 35 / 0.71 / 0.59
site (treatment) X time / 4 / 35 / 1.20 / 0.26