Supplementary Information

Supplementary Figure 1

w-gary1 MEMEHAVIQQQVVEYPEEHEVDVDVDN--IMKTEVAYGSGVHSTAFRYKHKKRSKVWEEY

w-gary2 MEMEHAVIHHQVVDYPEDLEVEVEVDLYNIMKTEVAYGSGVHSTAFRYKHKKRSKVWEEY

b-gary MEIEHAVIP-QVVEYTEEHEVEVDVDVDNIMKTEVSYGSGIHNSAFRYKHKKRSKVWEEY

**:***** ***:*.*: **:*:** ******:****:*.:****************

w-gary1 KPIFLNGKVQFAECLYCHNRLSCKDSNGTSHLWRHQKICPAKSEAAAAEGQQKDSYFPYV

w-gary2 KPIFLNGKVQFAECLYCHNRLSCKDSNGTSHLWRHQKICPAKSEAAAE-GQQKDSYFPYV

b-gary KPIFLDGKVQFAECLYCHNRLSCKDSNGTSHLWRHQKICPGKTEVAAE-RQQKDSYFPYV

*****:**********************************.*:*.** **********

w-gary1 LVNGDSPVSPIDPVNQIISETLEDIGSATPSRFKSKVWKEFTPIYVEGKLQAADCIHCQK

w-gary2 LVNGDGPLSPIDPVNQIISETLDDIGSTTPSRFKSKVWKEFTPIYVEGKLQAADCIHCQK

b-gary LLNGDSPVSPIDPVNQIISETLDDIGSATPSRFKSKVWKEFTPIYVEGKLQAADCIHCQK

*:***.*:**************:****:********************************

w-gary1 RLSANKFGGRSHLSRHLLTCPGRLESLHNQHNFLFEPLSAPSFNPRANDELSPALTNGKV

w-gary2 RLSANKFGGRSHLSRHLLTCPGRLESLHNHHKFLFEPSSAPSFNSRANDELSPALTNGKV

b-gary RLSANKFGGRSHLSRHLLTCPGRLESIHNHQKFLFEPSSAPSFDSRVHDELSPALTNGKV

**************************:**:::***** *****:.*.:************

w-gary1 QIAEYANKLPRTSTSGDTIPIVQPIQMLPAHPPPTSDGASLKKQRTSFTNISTDKSAGKF

w-gary2 QIAEYANKLPRISTSGDTIHTIQPIQMVPAHPPPTSDGASLKKQRTSFTNISTDKSAGKF

b-gary QIAEYTNKFPRTSTSAHTIPIVQPIQMLPAHTPPTSDGASLKKQKTSFTNISTDKRAGKF

*****:**:** ***..** :*****:***.************:********** ****

w-gary1 CQETSYQELAKMLLLHSYPFSVVEHEEMRRVIKNLNPMVGMVSXNDMKDHCTALFQKEKV

w-gary2 CQETSYQELAKMIILHAYPFSVVEHEEMRRVIKNLNPIVGMVSHNDMKDHCTALFQXEKV

b-gary CQETSYQELAKMIILHAYPFSIVEHEEMRRVIKNLNPVVGMVSHKDVEDHCIALFQKEKV

************::**:****:***************:***** :*::*** **** ***

w-gary1 NIKDVLTLSSRRVSLSASIWTPDGSEPTVNYLCLTAHFVGDDWKVHRVIIKFGMFWSSPT

w-gary2 NIKDVLALSSRRVSLSASIWTPDGSEPTVNYLCLTAHFVGDDWKVHRVIIKFGMFWSSPT

b-gary NIKDVITLSSRRVSLSASIWTPDGSDPTVNYLCLTAHFVGDDWKVHRVIIKFGMFWSSPT

*****::******************:**********************************

w-gary1 NLERIIHCKEASVPESESGSYNVMWDAIRDWNLDQKILSLTSVGDVRNNANTVKLKEMLI

w-gary2 NLERIIHCKEASVPESESGSYNVMWDAIRDWNLDQKILSLTSVGDVRNNANTVKLKEMLI

b-gary NLERIIYCKEASVPESESGSYNVIWDAIRDWNLDQKILSLTSVGDVRNNANTVKXKEMLI

******:****************:****************************** *****

w-gary1 EKKCLPIRGKLYNIACLDDILNSVVSAGQADILRFVGDAVMEFFVAHASSPSAQQQLLGV

w-gary2 EKKCLPIRGKLYNIACLDDILNSVVSAGQADILRFVGDAVMEFFVAHASSPSAQQQLLEV

b-gary EKKCLPIRGKLYNIACLDDILNSVVSAGQADILRFVGDVLMEFFVAHASSASAQQQLLEV

**************************************.:**********.******* *

w-gary1 ISQMSLKCPQENAKWWHKFYFRLEVLLHFKKSFPSEQVLNPEDMNVAESICKNLRTFYRV

w-gary2 ISQMSLKCPQEDAKWWHKFYFRLEVLLHFKKSFPSEEVLNLEDMNVAESICKILRMFYRV

b-gary VSQMSLKCPQEDAKWWHKFYFRLEVILHFKKSFPSEEVLNPEDMNVAESICKILRTFYRV

:**********:*************:**********:*** *********** ** ****

w-gary1 IEVISCPSSVTANIYFNEVWKVRTVLQEEASNDNGEFSRMVMEMQETFHGYWQNSYLWLS

w-gary2 IEVISCPSSVTANIYFNEVWKVRTVLHEEAPNGNGEFSRMVMEMQETFHGYWQNSYLWLS

b-gary IEVISCPSSVTANMYFNEVWKVRTVLQEEASNGNGEYSRLVMQMQESFHEYWQNSYLSLS

*************:************:***.*.***:**:**:***:** ******* **

w-gary1 IPVVLDPRFKIGFIEFRLKRAFGTNSESYLSDIRETVRELFNEYCNPTDQPSAASNIAAL

w-gary2 IPVVLDPKFKIGFIEFRLKRAFGTNSESYLSDIRETVRELFNEYCNPTDQPSAASNSAAL

b-gary IPVVLDPRFKISFIEFRLKRAFGTNSESYLSEIRDTVRELFNEYCNPTDRPSTASNSAAL

*******:***.*******************:**:**************:**:*** ***

w-gary1 DVDN-DLLEDWDHHLNEQASCQISTELDDYLEEGLVPRKDEFDILNWWMNHTAKYPTLAA

w-gary2 DVDN-DLLEDWDQHLNEQASCQISTELDDYLEEGLVPRKDEFDILNWWMNHTAKYPTLAA

b-gary GADYNDSLEDWDQHLNEQAGSQISTELDDYLEEGLVPRKDEFDILNWWMNHTEKYPTLAA

..* * *****:******..******************************* *******

w-gary1 MAQDILAMPASAVQSEAAFSSSGPVIPKHYSTLSIKTIEALVCARDWMR

w-gary2 MAQDILAMPASAVQSEAAFSSSGPVIPKHYSTLNIKTIEALVCARDWMR

b-gary MAQDILAMPASAVQSEAAFSSSGPVIPKHYSTLSIKTIEALVCARDWMR

*********************************.***************

ClustalW alignment of gary predicted transposase proteins of barley and wheat. Sequence identities are shown by (*), strongly conservative amino acid substitutions by (:) and weakly conservative substitutions by (.). The SVE motif discussed in the text is boxed in grey.

Supplementary Figure 2

Wis TCCATTTGCATAGAGTTTCTTCATGCATAAACACCAATAGACATGGTTCGCATGTCTCAA

B-gary TTCATTTGCATGAAGTTTCTTCATGCATTTACACCAACAGGCATGATTCACATGTCTCAA

HS858 TTCATTGGCATGAAGTTN-TTCATGCATTTACACCAACAGGCATGATTCACATGTNTCAA

Haruna T-CATT-GCAGGAAGTTN-TTCATGCATT-ACNCCAACAGGCANG-TTCA---ATTTTAA

Morex TTCATTTGCATGAAGTTTNTTCATGCATTTACACCAACAGGCATGATTCACATGTNTCAA

Galleon TCCATTTGCATGGAGTTTTT-CATGCATT-ACACCAACAGGCANG-TTCGCATGTTTCAA

Derkado TCCATNTGCATGGAGTTNTT-CATGCATTTACACCAACAGGCATGGTTCGCATGTCTCAA

Chime TCCATTTGCANGGAGTTTNTTCATGCATTTACACCAACAGGCATGGTTCGCATGTNTCAA

HS835 TCCATTTGCATGGAGTTTNTTCATGCATTTACACCAACAGGCATGGTTCGCATGTCTCAA

Franka TCCATNTGCATGGAGTTTNTTCATGCATTTACACCAACAGGCATGGTTCGCATGTCTCAA

Steptoe TCCATNTGCANGGAGTTTNTTCATGCATTTACACCAACAGGCATGGTTCGCATGTNTCAA

Mt TC--ATTGCA-GGGNTTTTTTCATGCATTTACACCAACAGGCATGGTTNGCATGTTTCAA

Cyrrhus TCCATCTGCA-GGAGTTTTT-CATGCATTTACACCANCAGGCATGGTTCGCATGTCTCAA

HS738 TCCATTTGCA-NGAGTTTTTTCATGCATTTACACCAACAGGCATGGTTCGCATGTCTCAA

HS702 TTC-TTNGCA-GAAGTTTTT-CA-GT-TTTACAC-AAC-GGCANG--TTGCAG---TTAA

B83 TTCATNTGCATGAAGTTCTT-CATGT-ATTACACCAACAGGCATGGTTCGCATGTCTCAA

HS700 ATCATGGGCANGAAGTTCTT-CANGTATTTACACCAACAGGCATGGTTCGCATGTNTCAA

Lina TCCATTTGCANGAAGTTTNTTCATGCATTTACANCAATAGACATGG-TCGCATGTTTCAA

*** ** * ** * * ** * * ** * * * **

Wis ACTTTTCAAAAATGAGTGAGCCTAAAGATCCATCAACATGGAGCTTCCTCATGCGTTCTA

B-gary ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTT-AAATTCCGAAACATTTTTT

HS858 ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTT-AAATTCCGAAACATTTTTT

Haruna ANCGTTCAGA-CCATTTT-TCGAANTT-CAGAACATTTTT---AATTCGGAAACATTTTT

Morex ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTT-AAATTCCGAAACATTTTTT

Galleon ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTTTAAATTCCCAACCATTTTTG

Derkado ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTTTAAATTCCCAAACATTTTTT

Chime ACGTTTCAGAACATTT-ATCGAAATTTCAGAACATTTTTTTAAATTCCCAAACATTTTTG

HS835 ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTTTAAATTCCCAAACATTTTTT

Franka ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTTTAAATTCCCAAACATTTTTT

Steptoe ACGTTTCAGAACATTTTATCGAAATTTCAGAACATTTTTTTAAATTCCCAAACATTTTTT

Mt A-GTTTCAGAACATTTTAT-GGAANTTCAGAACATTTTTTAAA--TCCCAAACATTTTTT

Cyrrhus ACGTTTCAGAACATTTTATNGAAATTTCAGAACATTTTTTTAAATTCCCAAACATTTTT-

HS738 ACATTTCAGAACATTTTATCAAAATTTCAGAACATTTTTTAAAATTCCGAATCATTTTTT

HS702 ACGTT-CAGA-CATTT-ATGGAN--TTCAGANT-TTTTT---AATTTCGAA-CATTTTT-

B83 ACGTTTCAGAACATTTTATCGAAATTTCAGAAC-TTTTTTTAAATTCCGAAACATTTTT-

HS700 ACGTTTCAGAACATTTTATNGAAATTTCAGAAT-TTTTTTTAAATTCCGAAACATTTTTT

Lina ANGTT-CA------AAACATTTTTTCAAATTCCGGAACATTTTT-

** ** * ***** * * * *******

B-gary GAAAATCCAGAACATTTTTAAAAAGAAACGAAAAAA-TAAAGAAAAAAGAAAACGAAAAT

HS858 GAAAATCCAGAACATTTTTAAAAAGAAACGAAAAAA-TAAAGAAAAAAGAAAACGAAAAT

Haruna GAAAATCCAGA-CATTTTTNAAATGNT-CGAAAAAN-TAAAGAAAAA--GAAGCGAAAAC

Morex GAAAATCCAGAACATTTTTAAAAAGAAACGAAAAAA-TAAAGAAAAAAGAAAACGAAAAT

Galleon GAAAATCCAGACCATTTT--GAAAGGAACGGAAAAAAATAAGGAAAAA---AACGAAATT

Derkado GAAAATCCAGAACATTTTTGAAAAGAAACGAAAAAAATAAAGAAAAAA---AACGAAAAT

Chime GAAAATCCAGAACATTTTTGAAAAGAAACGAAAAAAATAAAGAAAAAA---AACGAAAAT

HS835 GAAAATCCAGAACATTTTTGAAAAGAAACGAAAAAAATAAAGAAAAAA---AACGAAAAT

Franka GAAAATCCAGAACATTTTTGAAAAGAAACGAAAAAAATAAAGAAAAAA---AACGAAAAT

Steptoe GAAAATCCAGACCATTTTTGAAAAGAAACGAAAAAAATAAAGAAAAAA---AACGAAAAT

Mt GAAAATCCAGAACATTTT--GAAAAGAACGAAAAAAATAAAGAAAAAA---ANCGAAAAT

Cyrrhus GAAAATCCAGACCATTTT-GAAAAGAAACGAAAAAAATAAAGAAAAAA---AACGAAAAT

HS738 AAAAATACAGAACATTTTTGAAAAGAAATGAAAAAAATAAAGAAAAAAGAAAACGAAAAT

HS702 GAAA-TCCAGACCTTTT----GAAGGAACGAAAAAT---AAGAAAAAG-AAANGGAAATT

B83 GAAAATCCAGAACATTTTTGAAAAGAAACGAAAAAATAAAGAAAAAAG-AAAACGAAAAT

HS700 GAAAATCCAAAACATTTTTGAAAAGAAACGAAAAAATAAAGAAAAAAG-AAAACGAAAAT

Lina GAAAATCCAGAACATTTTTGAAAGGAANCAAAAAAA-TAAATAAAAAAGAAAACGAAAAT

*** * ** * * *** * **** * **** * ****

B-gary TAAAGTAAAMCCGGTTCAGG-AATCTTCTAGAAAGTTYTCAAAMCGGGAAAAAAACGGTT

HS858 TAAAGTAAAACCGGTTCAGG-AATCTTCTAGAAAGTTCTCAAAACGGGAAAAAAACGGTT

Haruna CAAGGTAAATCCGGTTCNGG-ATTTTTNTAGAAAGTTCTCAAACCGGAAAAAAAACGGTT

Morex TAAAGTAAAACCGGTTCAGG-AATCTTCTAGAAAGTTCTCAAAACGGGAAAAAAACGGTT

Galleon AAAGGTAAAACCGGTTCAGGGATTCTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

Derkado AAAAGTAAAACCGGTTCAGGGAATCTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

Chime AAAAGTAAAACCGGTTCAGGGAATCTTNTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

HS835 AAAAGTAAAACCGGTTCAGGGAATCTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

Franka AAAAGTAAAACCGGTTCAGGGAATCTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

Steptoe AAAAGTAAAACCGGTTCAGGGAATCTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

Mt AAAAGTAAAACCGGTTCAGGGAATCTTNTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

Cyrrhus AAAAGTAAAACCGGTTCAGGGAATCTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

HS738 AAAAGTAAAACCGGTTCAGGGAATCTTCTAGAAAGTTGTCAAAACGGGAAAAAACCGGTT

HS702 TAAGGTAAA-CCGGTTCNGGNA--TCTTTAGAAAGTTCTCAAA-CGGAAAAAACCCGGTT

B83 TAAAGTAAAACCGGTTCAGGGAATCTTCTAGAAAGTTCTCA------AAACGGGTT

HS700 TAAAGTAAAACCGGTTCTGGGAATCTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

Lina AAAAGTAAAGCCGGTTCGGGTA-TNTTCTAGAAAGTTCTCAAAACGGGAAAAAACCGGTT

** ***** ******* ** * * ********* *** ** ****

B-gary GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGA-ATTTTAACGCAGTA

HS858 GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTAACGCAGTA

Haruna GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTAACGCAGTA

Morex GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTAACGCAGTA

Galleon GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

Derkado GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

Chime GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

HS835 GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

Franka GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

Steptoe GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

Mt AGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACACAGTA

Cyrrhus GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

HS738 GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

HS702 GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACGGATATTTTGACGCAGTA

B83 GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

HS700 GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

Lina GGAAAAGTTCGCTCGCTTCAAGGGATGGTCTGTTCGAAAGACCGATATTTTGACGCAGTA

***************************************** ** ***** ** *****

B-gary AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

HS858 AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Haruna AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Morex AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Galleon AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Derkado AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Chime AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

HS835 AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Franka AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Steptoe AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Mt AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Cyrrhus AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

HS738 AGCGTCATATAGGAGTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

HS702 AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

B83 AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

HS700 AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

Lina AGCGTCATATAGGATTTTCCCCTTTACCCAGCCCACATCAAAAGCGATAACAAGAAGCCC

************** *********************************************

B-gary ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

HS858 ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Haruna ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATNGACGAACTGG

Morex ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATNGACGAACTGG

Galleon ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Derkado ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Chime ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

HS835 ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Franka ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Steptoe ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Mt ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Cyrrhus ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

HS738 ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

HS702 ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATNGACGAACTGG

B83 ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

HS700 ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAACTGG

Lina ACTTGAGCCCGTCTTGATAGAACGAGCGAGCCCTGTACCCAGCCCAAATCGACGAAGTGG

************************************************* ****** ***

B-gary GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

HS858 GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Haruna GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Morex GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Galleon GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Derkado GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Chime GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

HS835 GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Franka GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Steptoe GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Mt GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Cyrrhus GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

HS738 GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

HS702 GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

B83 GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

HS700 GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTCGATGCCCTAATCGTGCCCGG

Lina GTCTACCCACCCACCCACCGCCCGCCCGCAGACCCAACTGGATGCCCTAATCGTGCCCGG

*************************************** ********************

B-gary AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCTCT

HS858 AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCTCT

Haruna AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCTCT

Morex AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATNTCAGGTGACCTCT

Galleon AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCCCT

Derkado AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCCCT

Chime AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCCCT

HS835 AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATNTCAGGTGACCCCT

Franka AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCCCT

Steptoe AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATNTCAGGTGACCCCT

Mt AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATNTCAGGTGACCCCT

Cyrrhus AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCCCT

HS738 AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATNTCAGGTGACCCCT

HS702 AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATNTCAGGTGACCCCT

B83 AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCCCT

HS700 AATCTCCCCTCCCCGGCCCTCGCAAGGCTCCCCCGGCGATCTCCATCTCAGGTGACCCCT

Lina AATCTCCCCTCCCCGGCCCTCGCAAGGCCCCCCCGTCGATCTCCATCTCAGGTGACCCCT

**************************** ****** ********** ********** **

B-gary CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

HS858 CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Haruna CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Morex CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Galleon CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Derkado CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Chime CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

HS835 CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Franka CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Steptoe CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Mt CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Cyrrhus CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

HS738 CCCCCTCGATTTCTCCTACAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

HS702 CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

B83 CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

HS700 CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACG

Lina CCCCCTCGATTTCTCCTGCAATTCCTCGATCTGGGTTACTCATGCGCGTTTAATCTGACA

***************** *****************************************

B-gary ATTTGGGGGTCTCTAGAAACCGCAGTCGATGCGATCCATCAGTATTTCCCCATCCCAAAT

HS858 ATTTGGGGGTCTCTAGAAACCGCAGTCGATGCGATCCATCAGTATTTCCCCATCCCAAAT

Haruna ATTTGGGGGTCTATAGAAACCGCAGTCGATGCGATCCATCAGTATTTCCCCATCCCAAAT

Morex ATTTGGGGGTCTCTAGAAACCGCAGTCGATGCGATCCATCAGTATTTCCCCATCCCAAAT

Galleon ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

Derkado ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

Chime ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

HS835 ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

Franka ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

Steptoe ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

Mt ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

Cyrrhus ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

HS738 ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

HS702 ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

B83 ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

HS700 ATTTGGGG-TCTCTAGAGGCCGCAGTCGATGCGATCCATCAGTGTTTCCCCATCCCAAAT

Lina ATTTGGGG-TCTCTAGAAGCCGCAATCGATGCGACCCATCAGTGTTTCCCCATCCCAAAT

******** *** **** ***** ********* ******** ****************

B-gary GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

HS858 GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Haruna GTGTAGGATCCGGGNTATNTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Morex GTGTAGGATTTGGGGGANTTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Galleon GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Derkado GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Chime GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

HS835 GTGTAGGATTNGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Franka GTGTAGGATTTNGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Steptoe GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Mt GTGTAGGATTTNGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Cyrrhus GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

HS738 GTGTAGGATTTGGGGGATTTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

HS702 GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

B83 GTGTAGGATTTGGGGGAATTTTTTGCGCCGATTCCTGTAGCCCGAGCGCCATCTACCTGC

HS700 GTGTAGGATTNGGGGGAATTTTTTGCTACGATTCCTGTAGCCCGAGCGCCATCTACCTGC

Lina GTGTAGGNTTTGGGGGANTTTTTTGCGCCGATTCCTGTAGCCCTAGCGCCATCTACCTGC

******* * ** * ******* *************** ****************

B-gary AGATCTT-GTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

HS858 AGATCTT-GTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

Haruna AGATNTTTGTATTTTTGTACTAGNTGTTNANCCTTCTCTGTTNGACGTAGCTNTATGCTA

Morex AGNTNTT-GTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

Galleon AGATC-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

Derkado AGATN-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

Chime AGATC-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

HS835 AGATC-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGNTA

Franka AGATC-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

Steptoe AGATN-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

Mt AGATG-TTGTGTTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

Cyrrhus AGATT-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTNTTNGACGTAGCTGTATGNTA

HS738 AGATG-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

HS702 AGATG-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

B83 AGATC-TTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTGTTCGACGTAGCTGTATGCTA

HS700 AGATGGTTGTATTTTTGTACTAGCTGTTTAGCCTGCTCTTTTCGACGTAGCTGTATGCTA

Lina AGATC-TTGTACTTTCGT---AGCTGTTTAGGCTGCTCTGTTCGACGTAGCTGTATGCCA

** * * ** *** ** ** **** * ** **** ** ********* **** *

B-gary GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTACTGTGCAGCCATG

HS858 GGTTGCTAGTAGCAGGCAAGANTAAATTCATGTGTANTGTGCANCCATG

Haruna GGTTNNTAGTAGCAGGCAAGAGGAAATTNATNTCT--NGNGCAGCCATG

Morex GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTA-NTANCAGCCATG

Galleon GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTACTG-GCAGCCATG

Derkado GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTACTG-GCAGCCATG

Chime GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTACTGTGCAGCCATG

HS835 GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTANTGTGCAGCCATG

Franka GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTANTGAGCAGCCATG

Steptoe GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTANTGTGNANCCATG

Mt GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTANTGGGCAGCCATG

Cyrrhus GGTTGNTAGTNGCAGGCAAGANTAANTTCATGTGTNNTGNGCNCCCATG

HS738 GGTTGCTAGTAGCAGGCAAGANTAAATTCATGTGTANTGTGCAGCCATG

HS702 GGTTGCTAGTAGCAGGCAAGANTAAATTCATGTGTACTGG-CAGCCATG

B83 GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTACTGG-CAGCCATG

HS700 GGTTGCTAGTAGCAGGCAAGACTAAATTCATGTGTACTGNGCAGCCATG

Lina GGTTGCTAGTAGCAGGCAGGACTAAATTCATGTGTANTGTGCANCCATG

**** **** ******* ** ** ** ** * * *****

ClustalW alignment of upstream regions flanking b-gary in diverse barley germplasm. Homology with the int gene of Wis-2-1A is shown by grey shading. The presumptive transcription start point (5’-most cDNA end) is boxed in red, intron 1 is boxed in green and the translational initiator codon is boxed in green.

Supplementary Figure 3

A B

Southern DNA gel blot analysis of w-gary sequences in transformed wheat and barley. A. Wheat: Lane 1, Bobwhite wheat (nontransformed). Lanes 2- 7 - transgenic Bobwhite isolates. B. Barley: Lane 1, cv Golden Promise (nontransformed). Lanes 2- 7 - transgenic cv Golden Promise isolates. Both DNA blots were probed with b-gary.

Supplementary Table 1: Physical BIN-Map positions in wheat for homologues of b-gary and genes flanking garys in barley and rice

Gene

/

A genome

/

B genome

/

D genome

Rice - BF482280 / C-2AL1 0 - 0.85 / 2BL2 0.36 - 0.50 / 2DL3 - 0.49
b-gary / C-2AL1 0 - 0.85 / 2BL6 0.89 - 1.00 / 2DL9 0.76 -1.00
Rice - BE445431 / C-2AL1 0 - 0.85 / 2BL6 0.89 - 1.00 / 2DL9 0.76 -1.00
Rice - BE403827 / - / 2BL2 0.36 - 0.50 / 2DL9 0.76 -1.00
Barley - Gene 24 / - / - / 2DL3 0.49 - 0,76
2DL9 0.76 - 1.00
Barley - Gene 45 / - / 2BL2 0.36 - 0.50
2BL4 0.50 - 0.89 / -
Barley - Gene 65 / - / - / 2DL3 0.49 - 0,76
2DL9 0.76 - 1.00

The order of rice genes in rows reflects the gene order (BE403827 is distal-most).

The order of barley genes is not known. Bin-map positions shared with gary are shaded.