Supplemental Table 1. Frequency of SFRP1 Protein Expression in 132 Human Tumour

Supplemental Table 1. Frequency of SFRP1 protein expression in 132 human tumour

subtypes. Normal and benign tissues are shown in bold and italic letters. In order to statistically analyse the loss of SFRP1 in malignant tumour tissues we selected entities derived from normal or benign tissues with high SFRP1-expression (moderate/strong >75%) and at least five analysed tissue samples (marked with an asterisk). AML: Acute myelogenous leukaemia. CNS: central nervous system. CML: chronic myelogenous leukaemia. GIST: gastrointestinal stromal tumour. MALT: mucosa-associated lymphoid tissue. NHL: non-Hodgkin’s lymphoma. PNET: primitive neuro-ectodermal tumour. TCC: transitional cell carcinoma.

Tissue type
(normal and benign tissues in bold and italic letters) / SFRP1 protein expression
No. of tumours analysable / Negative (%) / Weak (%) / Moderate (%) / Strong (%)
*Adrenal gland (n=51)
Adrenal gland adenoma / 13 / 0 / 0 / 7.7 / 92.3
*Adrenal gland carcinoma / 6 / 0 / 16.7 / 16.7 / 66.7
*Pheochromocytoma / 27 / 74.1 / 0 / 11.1 / 14.8
Anus (n=5)
Anus (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Anus, squamous cell cancer / 3 / 33.3 / 0 / 66.7 / 0
Brain (n=200)
Cerebrum / white substance (normal tissue) / 3 / 66.7 / 0 / 0 / 33.3
Cerebrum / grey substance (normal tissue) / 3 / 66.7 / 0 / 0 / 33.3
Meningeoma / 38 / 44.7 / 18.4 / 21.1 / 15.8
Ependymoma / 8 / 0 / 37.5 / 37.5 / 25.0
Astrocytoma / 28 / 21.4 / 7.1 / 39.3 / 32.1
Glioblastoma multiforme / 32 / 15.6 / 9.4 / 43.8 / 31.3
Oligodendroglioma / 15 / 20.0 / 6.7 / 26.7 / 46.7
Medulloblastoma / 4 / 50.0 / 0 / 25.0 / 25.0
Esthesioneuroblastoma / 2 / 0 / 50.0 / 50.0 / 0
Breast (n=213)
Breast (normal tissue) / 3 / 33.3 / 0 / 33.3 / 33.3
Breast, ductal cancer / 41 / 43.9 / 17.1 / 24.4 / 14.6
Breast, lobular cancer / 32 / 56.3 / 6.3 / 21.9 / 15.6
Breast, medullary cancer / 25 / 28.0 / 16.0 / 36.0 / 20.0
Breast, tubular cancer / 19 / 68.4 / 0 / 26.3 / 5.3
Breast, mucinous cancer / 21 / 33.3 / 0 / 28.6 / 38.1
Breast, apocrine cancer / 3 / 0 / 0 / 33.3 / 66.7
Breast, cribirform cancer / 4 / 50.0 / 25.0 / 25.0 / 0
Breast, Phylloides tumor / 12 / 66.7 / 0 / 33.3 / 0
CNS (n=62)
Schwannoma / 32 / 68.8 / 6.3 / 21.9 / 3.1
Malignant Schwannoma / 8 / 87.5 / 0 / 12.5 / 0
*Colon (n=199)
Colon mucosa (normal tissue) / 2 / 0 / 0 / 0 / 100.0
Colon adenoma, mild dysplasia / 30 / 6.7 / 0 / 30.0 / 63.3
Colon adenoma, moderate dysplasia / 33 / 3.0 / 9.1 / 12.1 / 75.8
Colon adenoma, severe dysplasia / 24 / 0 / 4.2 / 16.7 / 79.2
*Colon, adenocarcinoma / 40 / 30.0 / 7.5 / 32.5 / 30.0
*Endometrium (n=74)
Endometrium (normal tissue) / 17 / 17.6 / 5.9 / 47.1 / 29.4
*Endometrium, endometrioid carcinoma / 39 / 51.3 / 7.7 / 20.5 / 20.5
*Endometrium, serous carcinoma / 18 / 27.8 / 11.1 / 55.6 / 5.6
Esophagus (n=47)
Esophagus (normal tissue) / 7 / 85.7 / 14.3 / 0 / 0
Esophagus, adenocarcinoma / 6 / 33.3 / 0 / 50.0 / 16.7
Esophagus, squamous cell carcinoma / 28 / 50.0 / 7.1 / 25.0 / 17.9
Esophagus, small cell carcinoma / 1 / 100.0 / 0 / 0 / 0
Fat tissue (n=30)
Fat tissue (normal tissue) / 3 / 100.0 / 0 / 0 / 0
Liposarcoma / 26 / 84.6 / 7.7 / 3.8 / 3.8
*Gall bladder (n=32)
Gall bladder (normal tissue) / 16 / 18.7 / 6.3 / 56.3 / 18.7
*Gall bladder, adenocarcinoma / 23 / 43.5 / 4.3 / 30.4 / 21.7
GIT (n=31)
(normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
GIST / 28 / 32.1 / 17.9 / 17.9 / 32.1
Hematologic (n=6)
(normal) / n.d. / n.d. / n.d. / n.d. / n.d.
AML / 1 / 0 / 0 / 0 / 100.0
CML / 4 / 0 / 0 / 0 / 100.0
*Kidney (n=139)
Kidney (normal tissue) / 9 / 0 / 0 / 0 / 100.0
Kidney, oncocytoma / 7 / 0 / 0 / 28.6 / 71.4
*Kidney, clear cell cancer / 47 / 42.6 / 8.5 / 23.4 / 25.5
*Kidney, papillary cancer / 34 / 26.5 / 5.9 / 38.2 / 29.4
*Kidney, chromophobe cancer / 11 / 0 / 45.5 / 27.3 / 27.3
Larynx (n=50)
Larynx (normal tissue) / n.d. / n..d. / n.d. / n.d. / n.d.
Larynx, squamous cell carcinoma / 35 / 37.1 / 5.7 / 25.7 / 31.4
*Liver (n=50)
Liver (normal tissue) / 3 / 0 / 0 / 0 / 100.0
*Hepatocellular carcinoma / 28 / 3.6 / 0 / 0 / 96.4
Lung (n=200)
Lung (normal tissue) / 4 / 100.0 / 0 / 0 / 0
Lung, squamous cell carcinoma / 49 / 93.9 / 2.0 / 4.1 / 0
Lung, adenocarcinoma / 46 / 87.0 / 2.2 / 2.2 / 8.7
Lung, large cell cancer / 42 / 90.5 / 0 / 4.8 / 4.8
Lung, small cell cancer / 38 / 100.0 / 0 / 0 / 0
Lymphatic tissue (n=127)
Lymphatic tissue (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
NHL, diffuse large B / 16 / 56.3 / 6.3 / 31.3 / 6.3
MALT lymphoma / 29 / 100.0 / 0 / 0 / 0
Hodgkin lymphoma, mixed cell / 14 / 71.4 / 0 / 28.6 / 0
Hodgkin lymphoma, nodular sclerosis / 28 / 67.9 / 10.7 / 21.4 / 0
Lymph node (n=20)
Lymph node (normal tissue) / 2 / 100.0 / 0 / 0 / 0
NHL, others / 12 / 75.0 / 0 / 25.0 / 0
Mouth (n=50)
Oral cavity (normal tissue) / 4 / 75.0 / 0 / 0 / 25.0
Oral cavity, squamous cell carcinoma / 40 / 12.5 / 5.0 / 42.5 / 40.0
Myometrium (n=61)
Leiomyoma / 57 / 100.0 / 0 / 0 / 0
Nerve tissue (n=43)
Neurofibroma / 22 / 95.5 / 4.5 / 0 / 0
Ovary (n=135)
Ovary (normal tissue) / 4 / 100.0 / 0 / 0 / 0
Ovary, Brenner tumour / 9 / 100.0 / 0 / 0 / 0
Ovary, serous cancer / 42 / 71.4 / 4.8 / 19.0 / 4.8
Ovary, mucinous cancer / 14 / 42.9 / 0 / 35.7 / 21.4
Ovary, endometrioid cancer / 42 / 92.9 / 2.4 / 2.4 / 2.4
Ovary, dysgerminoma / 2 / 100.0 / 0 / 0 / 0
Ovary, yolk sack tumour / 1 / 0 / 0 / 0 / 100.0
Ovary, undifferentiated carcinoma / 1 / 0 / 0 / 100.0 / 0
*Pancreas (n=50)
Pancreas (normal tissue) / 9 / 0 / 0 / 22.2 / 77.8
*Pancreas, adenocarcinoma / 43 / 41.9 / 7.0 / 25.6 / 25.6
*Parathyroid (n=32)
Parathyroid (normal tissue) / 3 / 0 / 0 / 0 / 100.0
Parathyroid, adenoma / 17 / 29.4 / 0 / 5.9 / 64.7
Parotis (n=10)
Parotis (normal tissue) / 5 / 80.0 / 0 / 20.0 / 0
Salivary gland, small cell cancer / 1 / 100.0 / 0 / 0 / 0
Salivary gland, squamous cell cancer / 2 / 100.0 / 0 / 0 / 0
Salivary gland, unclassified carcinoma / 1 / 0 / 0 / 0 / 100.0
Salivary gland, undifferentiated carcinoma / 4 / 50.0 / 0 / 50.0 / 0
Penis (n=50)
Penis (normal tissue) / 5 / 60.0 / 0 / 40.0 / 0
Penile carcinoma / 33 / 12.1 / 9.1 / 48.5 / 30.3
Pharynx (n=5)
Pharynx (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Pharynx, lymphoepithelial carcinoma / 3 / 33.3 / 0.0 / 33.3 / 33.3
Pituitary (n=8)
Craniopharyngeoma / 4 / 75.0 / 0 / 0 / 25.0
Pleura (n=28)
(normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Malignant mesothelioma / 15 / 40.0 / 13.3 / 20.0 / 26.7
Prostate (n=110)
Prostate (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Prostate cancer, untreated / 48 / 25.0 / 6.3 / 27.1 / 41.7
Prostate cancer, hormone refractory / 30 / 40.0 / 0 / 6.7 / 53.3
*Salivary gland (n=152)
Salivary gland, adenolymphoma / 28 / 14.3 / 0 / 0 / 85.7
Salivary gland, pleomorphic adenoma / 48 / 60.4 / 12.5 / 14.6 / 12.5
Salivary gland, cylindroma / 41 / 46.3 / 22.0 / 19.5 / 12.2
Salivary gland, mucoepidermoid cancer / 3 / 33.3 / 0 / 0 / 66.7
Salivary gland, adenocarcinoma / 1 / 100.0 / 0 / 0 / 0
*Salivary gland, acinus cell cancer / 6 / 50.0 / 0 / 16.7 / 33.3
Skeletal muscle (n=14)
Skeletal muscle (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Rhabdomyosarcoma / 10 / 40.0 / 0 / 40.0 / 20.0
Skin (n=301)
Skin (normal tissue) / 12 / 8.3 / 25.0 / 66.7 / 0
Skin, benign nevus / 22 / 27.3 / 22.7 / 31.8 / 18.2
Benign histiocytoma / 21 / 100.0 / 0 / 0 / 0
Dermatofibroma protuberans / 1 / 100.0 / 0 / 0 / 0
Kapillary hemangioma / 19 / 89.5 / 5.3 / 5.3 / 0
Skin, basalioma / 31 / 45.2 / 16.1 / 25.8 / 12.9
Skin, squamous cell cancer / 31 / 22.6 / 12.9 / 35.5 / 29.0
Skin, Merkel cell cancer / 3 / 0 / 0 / 33.3 / 66.7
Skin, malignant melanoma / 37 / 35.1 / 16.2 / 29.7 / 18.9
Kaposi Sarcoma / 18 / 72.2 / 5.6 / 16.7 / 5.6
Skin appendix (n=32)
Skin, benign appendix tumor / 20 / 55.0 / 10.0 / 25.0 / 10.0
*Small intestine (n=17)
Small intestine (normal tissue) / 16 / 18.7 / 6.3 / 56.3 / 18.7
*Small intestine, adenocarcinoma / 11 / 36.4 / 9.1 / 18.2 / 36.4
Smooth muscle (n=40)
Smooth muscle (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Leiomyosarcoma / 37 / 78.4 / 13.5 / 8.1 / 0
Soft tissue (n=193)
Lipoma / 24 / 100.0 / 0 / 0 / 0
Epitheloid Hemangioma / 1 / 100.0 / 0 / 0 / 0
Glomus tumor / 5 / 20.0 / 0 / 20.0 / 60.0
Ganglioneuroma / 7 / 85.7 / 0 / 14.3 / 0
Angiomyolipoma / 1 / 0 / 100.0 / 0 / 0
Paraganglioma / 6 / 16.7 / 50.0 / 16.7 / 16.7
Malignant fibrous histiocytoma / 22 / 77.3 / 4.5 / 13.6 / 4.5
Fibrosarcoma / 8 / 37.5 / 0 / 37.5 / 25.0
Synovial Sarcoma / 2 / 0 / 0 / 50.0 / 50.0
Alveolar Sarcoma / 1 / 0 / 0 / 100.0 / 0
Epitheloid Sarcoma / 2 / 50.0 / 0 / 0 / 50.0
Hemangiopericytoma / 4 / 0 / 0 / 75.0 / 25.0
Angiosarcoma / 3 / 66.7 / 0 / 33.3 / 0
Granular cell tumour / 5 / 40.0 / 0 / 20.0 / 40.0
PNET / 14 / 35.7 / 14.3 / 35.7 / 14.3
*Stomach (n=76)
Stomach (normal tissue) / 16 / 25.0 / 0 / 56.3 / 18.7
*Stomach, diffuse adenocarcinoma / 22 / 22.7 / 4.5 / 40.9 / 31.8
*Stomach, intestinal adenocarcinoma / 40 / 35.0 / 12.5 / 25.0 / 27.5
Tendon sheet (n=36)
Tendon sheet (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Tendon sheet, giant cell tumour / 22 / 59.1 / 22.7 / 9.1 / 9.1
*Testis (n=109)
Testis (normal tissue) / 18 / 0 / 0 / 61.1 / 38.9
*Testis, seminoma / 46 / 54.3 / 4.3 / 17.4 / 23.9
*Testis, non-seminomatous cancer / 51 / 41.2 / 9.8 / 21.6 / 27.5
Testis, mixed cancer / 2 / 50.0 / 50.0 / 0 / 0
Testis, teratoma / 4 / 100.0 / 0 / 0 / 0
Thymus (n=24)
Thymus (normal tissue) / 3 / 100.0 / 0 / 0 / 0
Thymoma / 19 / 94.7 / 5.3 / 0 / 0
Thyroid (n=161)
Thyroid (normal tissue) / 3 / 33.3 / 0 / 66.7 / 0
Thyroid, adenoma / 36 / 8.3 / 2.8 / 19.4 / 69.4
Thyroid, follicular cancer / 43 / 16.3 / 2.3 / 11.6 / 69.8
Thyroid, papillary cancer / 35 / 20.0 / 11.4 / 17.1 / 51.4
Thyroid, anaplastic cancer / 5 / 0 / 0 / 40.0 / 60.0
Thyroid, medullary cancer / 8 / 12.5 / 12.5 / 12.5 / 62.5
Urinary bladder (n=130)
Urinary bladder (normal tissue) / 2 / 50.0 / 0 / 0 / 50.0
Urinary bladder cancer, non-invasive urothelial cancer / 28 / 3.6 / 3.6 / 46.4 / 46.4
Urinary bladder cancer, invasive urothelial cancer / 32 / 31.3 / 9.4 / 21.9 / 37.5
Urinary bladder, squamous cell cancer / 4 / 50.0 / 25.0 / 25.0 / 0
Urinary bladder, small cell cancer / 3 / 33.3 / 0 / 66.7 / 0
Urinary bladder, sarcomatoid cancer / 6 / 33.3 / 16.7 / 50.0 / 0
Urinary bladder, adenocarcinoma / 2 / 100.0 / 0 / 0 / 0
Uterus (n=10)
(Normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Uterus, carcinosarcoma / 5 / 0 / 0 / 60.0 / 40.0
Endometrioid stroma sarcoma / 3 / 100.0 / 0 / 0 / 0
Uterus, cervix (n=83)
Cervix (normal tissue) / 2 / 50.0 / 0 / 50.0 / 0
Uterus, cervix, cervical intraepithelial neoplasia, grade 3 / 9 / 11.1 / 22.2 / 55.6 / 11.1
Uterus, cervix, squamous cell carcinoma / 20 / 20.0 / 10.0 / 20.0 / 50.0
Uterus, cervix, adenocarcinoma / 2 / 50.0 / 0 / 50.0 / 0
Vagina (n=5)
Vagina, squamous cell (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Vagina, squamous cell carcinoma / 3 / 66.7 / 0 / 33.3 / 0
Vulva (n=45)
Vulva, squamous cell (normal tissue) / n.d. / n.d. / n.d. / n.d. / n.d.
Vulva, squamous cell cancer / 32 / 43.8 / 18.8 / 28.1 / 9.4

* = High expression of SFRP1 in normal or benign tissues (moderate/strong>75%) see Table 1

n.d. = not determined