Supplemental Data Table2 Relative quantification of expression of PxylGOBP1 and PxylGOBP2 amplified at varioustimes by real-time PCR

Am0hr / Am4hr / Am8hr / Am16hr / AmMated / Af0h / Af4hr / Af8hr / Af16hr / AfMated
CtPxylGOBP11 / 21.9682 / 22.8209 / 21.1762 / 20.8121 / 21.5064 / 20.865 / 22.0437 / 21.8615 / 22.3419 / 22.6047
CtPxylGOBP1(-(Ct-B)/K) / 2.0498 / 1.7874 / 2.2935 / 2.4055 / 2.1919 / 2.3892 / 2.0266 / 2.0826 / 1.9348 / 1.8387
CtPxylActin(-(Ct-B)/K) / 1.907161 / 1.889097 / 2.088032 / 2.518839 / 1.90671 / 3.100806 / 1.881968 / 2.551097 / 2.405613 / 1.519419
△Ct / 0.1426 / -0.1017 / 0.2054 / -0.1133 / 0.2852 / -0.7116 / 0.1446 / -0.4685 / -0.4708 / 0.3193
10△Ct / 1.39 / 0.79 / 1.60 / 0.77 / 1.93 / 0.19 / 1.40 / 0.34 / 0.34 / 2.09
CtPxylGOBP12 / 21.9562 / 22.8506 / 21.1689 / 20.8025 / 21.5201 / 20.8014 / 22.0561 / 21.561 / 22.2145 / 22.6014
CtPxylGOBP1(-(Ct-B)/K) / 2.0535 / 1.7783 / 2.2957 / 2.4085 / 2.1877 / 2.4088 / 2.0227 / 2.1751 / 1.9740 / 1.8550
CtPxylActin(-(Ct-B)/K) / 1.9002 / 1.9098 / 2.1197 / 2.5562 / 1.9083 / 3.1655 / 1.9111 / 2.4374 / 2.4444 / 1.5131
△Ct / 0.1533 / -0.1315 / 0.1760 / -0.1477 / 0.2793 / -0.7567 / 0.1116 / -0.2623 / -0.4704 / 0.3419
10△Ct / 1.42 / 0.74 / 1.50 / 0.71 / 1.90 / 0.18 / 1.29 / 0.55 / 0.34 / 2.20
CtPxylGOBP13 / 21.8915 / 22.7901 / 21.8695 / 20.7641 / 21.951 / 20.2561 / 22.7475 / 21.7821 / 22.0124 / 22.2041
CtPxylGOBP1(-(Ct-B)/K) / 2.0734 / 1.7969 / 2.0802 / 2.4203 / 2.0551 / 2.5766 / 1.8100 / 2.1070 / 2.0362 / 1.9772
CtPxylActin(-(Ct-B)/K) / 1.9284 / 1.8657 / 2.0945 / 2.5747 / 1.9022 / 3.0554 / 1.8329 / 2.4652 / 2.3361 / 1.5248
△Ct / 0.1450 / -0.0688 / -0.0144 / -0.1545 / 0.1529 / -0.4788 / -0.0229 / -0.3581 / -0.2999 / 0.4524
10△Ct / 1.40 / 0.85 / 0.97 / 0.70 / 1.42 / 0.33 / 0.95 / 0.44 / 0.50 / 2.83
average / 1.4028 / 0.7945 / 1.3573 / 0.7276 / 1.7509 / 0.2338 / 1.2123 / 0.4417 / 0.3927 / 2.3724
stdev / 0.0181 / 0.0575 / 0.3418 / 0.0374 / 0.2853 / 0.0856 / 0.2339 / 0.1034 / 0.0941 / 0.4038
SE / 0.0105 / 0.0332 / 0.1973 / 0.0216 / 0.1647 / 0.0494 / 0.1350 / 0.0597 / 0.0543 / 0.2331
CtPxylGOBP21 / 19.559 / 20.4671 / 19.2022 / 18.0688 / 19.1255 / 18.3469 / 19.7383 / 19.6562 / 20.1661 / 21.1735
CtPxylGOBP2(-(Ct-B)/K) / 2.7315 / 2.4337 / 2.8485 / 3.2201 / 2.8736 / 3.1289 / 2.6727 / 2.6996 / 2.5324 / 2.2021
CtPxylActin(-(Ct-B)/K) / 1.907161 / 1.889097 / 2.088032 / 2.518839 / 1.90671 / 3.100806 / 1.881968 / 2.551097 / 2.405613 / 1.519419
△Ct / 0.8243 / 0.5446 / 0.7604 / 0.7012 / 0.9669 / 0.0281 / 0.7907 / 0.1485 / 0.1268 / 0.6827
10△Ct / 6.67 / 3.50 / 5.76 / 5.03 / 9.27 / 1.07 / 6.18 / 1.41 / 1.34 / 4.82
CtPxylGOBP22 / 19.5691 / 20.6254 / 19.1025 / 18.2105 / 19.0256 / 18.3254 / 19.3651 / 19.2987 / 20.1598 / 21.5678
CtPxylGOBP2(-(Ct-B)/K) / 2.7282 / 2.3818 / 2.8811 / 3.1736 / 2.9064 / 3.1359 / 2.7950 / 2.8168 / 2.5345 / 2.0729
CtPxylActin(-(Ct-B)/K) / 1.9002 / 1.9098 / 2.1197 / 2.5562 / 1.9083 / 3.1655 / 1.9111 / 2.4374 / 2.4444 / 1.5131
△Ct / 0.8280 / 0.4721 / 0.7614 / 0.6174 / 0.9980 / -0.0296 / 0.8840 / 0.3795 / 0.0901 / 0.5598
10△Ct / 6.73 / 2.97 / 5.77 / 4.14 / 9.95 / 0.93 / 7.66 / 2.40 / 1.23 / 3.63
CtPxylGOBP23 / 19.4215 / 20.3245 / 19.0214 / 17.9025 / 19.3562 / 18.5684 / 19.6581 / 19.8753 / 20.1785 / 21.365
CtPxylGOBP2(-(Ct-B)/K) / 2.7766 / 2.4805 / 2.9077 / 3.2746 / 2.7980 / 3.0563 / 2.6990 / 2.6278 / 2.5284 / 2.1393
CtPxylActin(-(Ct-B)/K) / 1.9284 / 1.8657 / 2.0945 / 2.5747 / 1.9022 / 3.0554 / 1.8329 / 2.4652 / 2.3361 / 1.5248
△Ct / 0.8482 / 0.6148 / 0.8132 / 0.6998 / 0.8957 / 0.0009 / 0.8661 / 0.1626 / 0.1923 / 0.6146
10△Ct / 7.05 / 4.12 / 6.50 / 5.01 / 7.87 / 1.00 / 7.35 / 1.45 / 1.56 / 4.12
average / 6.82 / 3.53 / 6.01 / 4.73 / 9.03 / 1.00 / 7.06 / 1.75 / 1.38 / 4.19
stdev / 0.20 / 0.58 / 0.43 / 0.50 / 1.06 / 0.07 / 0.78 / 0.56 / 0.17 / 0.60
SE / 0.12 / 0.33 / 0.25 / 0.29 / 0.61 / 0.04 / 0.45 / 0.32 / 0.10 / 0.34

Note: Am: male antenna; Af: female antenna; 0, 4, 8, and 16 hr and mated refers to theadults at 0, 4, 8, and 16 hr after eclosion and mated.