Supplementary Table S1. Carbon source utilization by Hymenobacter-like VUG strains as determined using the Biolog GN systema. The order in which strains are presented is the same as that in Figure 1.
Compoundb / VUG-A130 / VUG-A42aa / VUG-A67 / VUG-A57b / VUG-A33 / VUG-A34 / VUG-A2a / VUG-A58 / VUG-A48 / VUG-A23a / VUG-A124 / VUG-A142 / VUG-A112α-Cyclodextrin / -c / - / - / w / - / wd / - / - / - / - / - / - / -
Dextrin / + / + / + / + / + / wd / + / + / + / wd / + / + / +
Glycogen / - / - / - / w / - / - / - / - / - / - / - / - / -
Tween 40 / - / + / - / - / + / - / + / + / - / + / + / + / -
N-Acetyl-D-galactosamine / - / - / w / - / - / - / - / - / - / - / - / - / -
D-Cellobiose / - / - / - / w / - / - / - / - / - / - / - / - / -
D-Fructose / - / - / - / - / - / - / - / w / - / - / - / - / -
D-Galactose / - / - / + / + / - / - / - / w / - / - / - / - / +
Gentiobiose / - / - / w / + / - / - / - / w / - / - / - / - / -
α-D-Glucose / - / - / wd / + / - / - / - / - / + / + / - / - / +
m-Inositol / - / - / - / + / - / - / - / - / - / - / - / - / -
α-D-Lactose / - / - / - / w / - / - / - / - / - / - / - / - / -
Maltose / - / + / wd / + / - / - / - / w / + / - / wd / - / +
Mannose / - / - / - / wd / - / - / - / - / - / - / - / - / -
D-Raffinose / - / - / - / wd / - / - / - / - / - / - / - / - / -
L-Rhamnose / - / - / - / w / - / - / - / - / - / - / - / - / -
D-Sorbitol / - / - / - / w / wd / - / - / wd / - / - / - / - / -
Sucrose / - / - / - / - / - / - / - / - / + / - / - / - / -
D-Trehalose / - / - / - / w / - / - / - / w / - / - / - / - / -
Xylitol / - / - / - / - / - / - / - / w / - / - / - / - / -
Pyruvate methyl ester / - / + / + / + / + / + / + / + / + / - / + / + / -
Succinate mono-methyl ester / - / + / w / + / + / + / - / + / - / - / - / - / -
Acetic acid / - / - / wd / wd / - / - / - / w / - / - / - / - / -
Citrate / - / - / - / w / - / - / - / - / - / - / - / - / -
D-Gluconate / - / - / - / - / wd / - / - / - / - / - / - / - / -
Compound / VUG-A130 / VUG-A42aa / VUG-A67 / VUG-A57b / VUG-A33 / VUG-A34 / VUG-A2a / VUG-A58 / VUG-A48 / VUG-A23a / VUG-A124 / VUG-A142 / VUG-A112
p-Hydroxyphenylacetate / + / - / - / - / - / - / - / - / - / - / - / - / -
α-Ketobutyrate / - / + / + / + / + / + / + / + / - / + / + / + / -
α-Ketoglutarate / - / + / + / wd / wd / - / + / + / - / + / + / + / -
α-Ketovalerate / w / + / + / + / + / + / + / + / - / + / + / + / -
Malonate / - / - / - / w / - / - / - / w / - / - / - / - / -
D-Saccharate / - / - / - / w / - / - / - / - / - / - / - / - / -
Succinate mono-methyl ester / - / - / - / - / wd / - / - / - / - / - / - / - / -
L-Alaninamide / - / + / w / - / - / + / - / - / - / - / - / - / -
L-Alanine / - / + / + / w / wd / + / - / w / - / - / - / - / -
L-Alanylglycine / - / + / + / + / - / + / - / - / - / - / - / - / -
L-Asparagine / - / + / - / - / - / + / - / w / - / - / - / - / -
L-Aspartate / - / wd / + / - / - / + / - / wd / - / - / - / - / -
L-Glutamate / - / + / + / w / + / + / + / + / + / - / + / + / +
Glycyl-L-aspartate / w / - / wd / w / - / - / - / - / - / - / - / - / -
Glycyl-L-glutamate / - / - / + / + / - / + / - / - / - / - / - / - / +
L-Ornithine / - / + / + / wd / - / + / - / wd / + / - / - / - / -
L-Proline / w / + / + / w / + / + / + / + / - / - / - / - / -
D-Serine / - / - / wd / - / - / - / - / - / - / - / - / - / -
L-Serine / w / - / + / - / - / + / - / - / - / - / - / - / -
L-Threonine / - / + / + / w / + / + / - / w / - / - / - / - / -
Uridine / - / - / wd / + / - / + / - / - / - / - / - / - / -
aAllstrains were grown on R2A agar at 18°C in the dark for and analyzed after 1 and 2 weeks.
bCompounds not metabolized by any tested strain: N-acetyl-D-glucosamine;adonitol; D-alanine;γ-aminobutyrate; 2-aminoethanol; D-arabitol;bromosuccinate; 2,3-butanediol; D,L-carnitine;i-erythritol;formate; L-fucose; D-galacturonate; D-galactonate lactone;D-glucose-6-phosphate;α-D-glucose-1-phosphate;glucuronamide; D-glucuronate;glycerol; D,L-α-glycerol phosphate; L-histidine;α-hydroxybutyrate;β-hydroxybutyrate;γ-hydroxybutyrate;hydroxyl-L-proline;inosine;D,L-lactate;lactulose; D-mannitol;β-methyl-D-glucoside;phenylethylamine;propionate; D-psicose;putrescine; L-pyroglutamate;quinate;sebacate;turanose;tween 80;thymidine;urocanate
c(-): Negative result; (w): Weak positive result; (+): Strong positive result
dThese results differed between analyses conducted after 1 or 2 weeks of incubation, most likely due to the proximity of Biolog signal reading to the automatically determined cutoff characterizing each test as (-), (w) or (+). These results are therefore uniformly classified as weak positives for simplicity
Supplementary Table S2. Percentage fatty acid compositions of VUG Hymenobacter-like strainsa. Compounds are listed in the order in which they eluted, and only those present in at least one strain at ≥ 1% of the total FAME content are reported. The order in which strains are presented is the same as that in Figure 1.
FAME / VUG-A130 / VUG-A42aa / VUG-A67 / VUG-A57b / VUG-A33 / VUG-A34 / VUG-A2a / VUG-A58 / VUG-A48 / VUG-A23a / VUG-A124 / VUG-A142 / VUG-A112C14:0 iso / 1.1 / 1.3 / - / 1.1 / tr / 2.3 / trb / tr / tr / -c / tr / - / -
C14:0 / tr / 1.3 / tr / tr / tr / 1.2 / tr / tr / tr / - / tr / tr / tr
C15:1 iso G / 10.5 / tr / tr / 2.0 / 1.1 / 1.8 / 2.6 / 1.9 / 2.3 / - / tr / tr / 1.9
Sum In Feature 1d / - / tr / - / tr / - / 1.3 / tr / tr / 1.1 / - / 1.7 / 1.4 / 1.1
C15:1 anteiso A / 4.2 / tr / - / tr / tr / tr / tr / tr / 1.4 / - / - / - / -
C15:0 iso / 13.5 / 9.3 / 7.8 / 16.7 / 3.8 / 13.2 / 9.1 / 8.2 / 10.7 / 8.7 / 10.8 / 9.3 / 12.4
C15:0 anteiso / 19.6 / 10.5 / 10.4 / 8.5 / 3.3 / 16.3 / 9.3 / 8.0 / 14.6 / 2.2 / tr / - / -
C15:0 / tr / tr / - / tr / - / tr / tr / tr / - / - / 1.3 / 1.0 / -
C16:1 ω7c alcohol / - / 1.0 / 6.6 / - / tr / - / tr / tr / - / - / - / - / -
C16:1 iso H / tr / 1.1 / - / tr / 1.4 / 2.7 / 1.0 / tr / - / 2.7 / 3.1 / tr / -
C16:0 iso / - / - / - / - / - / - / - / - / - / - / 2.8 / - / -
Sum In Feature 2e / tr / 1.3 / 3.8 / tr / 1.0 / - / 1.3 / 1.4 / 2.3 / - / - / 1.3 / tr
Unknown ECLf 15.65 / 2.6 / 2.2 / 1.2 / 1.5 / 6.9 / 1.3 / 2.1 / 2.2 / 1.2 / 2.8 / - / 1.7 / 1.4
Unknown ECL 15.76 / tr / tr / tr / - / tr / tr / tr / tr / - / 3.4 / 2.6 / 4.0 / 5.1
Sum In Feature 3g / 20.3 / 35.4 / 34.7 / 32.4 / 43.8 / 26.4 / 34.8 / 41.7 / 32.0 / 23.0 / 24.2 / 26.0 / 13.9
C16:1 ω5c / 4.6 / 12.5 / 13.6 / 14.5 / 20.6 / 11.2 / 12.3 / 12.1 / 5.9 / 17.0 / 15.0 / 21.3 / 10.9
C16:0 / 2.5 / 7.5 / 4.7 / 3.6 / 1.8 / 3.3 / 3.1 / 2.6 / 4.4 / 5.4 / 3.1 / 4.0 / 5.1
C15:0 iso 3-OH / 1.7 / 2.5 / 2.9 / 2.8 / 1.7 / 2.8 / 3.5 / 3.2 / 3.9 / 3.4 / 2.6 / 3.2 / 4.0
Unknown ECL 16.35 / tr / - / - / - / - / - / - / - / - / 5.6 / 6.3 / 3.1 / 9.6
C15:0 2-OH / 1.2 / tr / 1.0 / - / tr / tr / 1.0 / tr / 2.4 / - / - / - / -
Sum In Feature 4h / 6.6 / 3.3 / 2.4 / 5.9 / 5.0 / 6.5 / 4.0 / 4.1 / 6.0 / 10.9 / 13.3 / 9.9 / 18.9
C17:0 iso / 2.5 / tr / - / tr / tr / - / tr / tr / tr / 2.1 / 1.1 / tr / 1.7
Unknown ECL 17.20 / - / - / - / - / - / - / - / - / - / - / 2.0 / 2.8 / tr
C16:0 iso 3-OH / 1.1 / 2.6 / 1.9 / 1.6 / 1.9 / 3.4 / 4.0 / 3.4 / 2.0 / - / - / - / -
C16:0 3-OH / tr / 2.3 / 4.2 / 2.7 / 1.1 / 2.2 / 3.1 / 3.2 / 3.6 / 2.4 / 1.2 / 2.7 / 1.3
C18:1 ω9c / - / - / - / - / - / - / - / - / - / 3.2 / 3.3 / 3.2 / 3.9
C18:0 / - / - / - / - / - / - / - / - / - / 3.3 / - / - / -
Sum In Feature 8i / - / - / tr / - / 1.3 / - / tr / tr / - / - / tr / - / -
C17:0 iso 3-OH / 1.7 / tr / tr / 1.3 / tr / tr / 1.3 / tr / 1.6 / - / 3.1 / 2.8 / 6.6
C17:0 2-OH / 2.4 / 1.3 / 1.9 / tr / tr / 1.2 / 1.4 / tr / 2.4 / 4.0 / - / - / -
aAllstrains were grown on R2A agar at 18°C in the dark for 1 week, except where indicated.
btr: Trace; present at < 1% of the total FAME content in this strain
c-: not detected
dSum In Feature 1 contains C15:1 iso H and C13:0 3-OH
eSum In Feature 2 contains C12:0 aldehyde, C16:1 iso I and C14:0 3-OH
fECL: Equivalent Chain Length, the standardized FAME identification measure used by the MIDI system
gSum In Feature 3 contains C16:1 ω7c and C16:1 ω6c
hSum In Feature 4 contains C17:1 iso I and C17:1 anteiso B
iSum In Feature 8 contains C18:1 ω7c and C18:1 ω6c
Supplementary Table S3. Carotenoid composition of VUG Hymenobacter-like strainsa. Values are expressed as the percentage of total carotenoids detected (mean of three independent replicates with one standard deviation).The order in which strains are presented is the same as that in Figure 1.
Strain / 2´-Hydroxyflexixanthin / 2´-Methoxyflexixanthin / 3´-Deoxy-2´-methoxyflexixanthin / Plectaniaxanthin / 2´-Methoxyplectaniaxanthin
VUG-A130 / 100 / -b / - / - / -
VUG-A42aac / 49 ± 2 / 40 ± 0.9 / 5.1 ± 0.6 / 3.3 ± 0.5 / 2.8 ± 0.3
VUG-A67c / 27 ± 0.3 / 68 ± 1 / 2.9 ± 0.6 / trd / 1.7 ± 0.2
VUG-A57b / 64 ± 1 / 29 ± 1 / 2.2 ± 0.1 / 3.4 ± 0.4 / 1.6 ± 0.2
VUG-A33c / 62 ± 2 / 37 ± 2 / tr / tr / tr
VUG-A34c / 79 ± 2 / 15 ± 2 / tr / 3.6 ± 0.3 / 2.1 ± 0.2
VUG-A2a / 93 ± 0.4 / 3.9 ± 0.2 / tr / 1.7 ± 0.1 / tr
VUG-A58 / 95 ± 2 / 4.4 ± 1 / - / 1.2 ± 0.2 / tr
VUG-A48 / 98 ± 1 / 1.6 ± 0.1 / - / tr / tr
VUG-A23ac / 95 ± 0.7 / 3.7 ± 0.4 / - / tr / -
VUG-A124c / 16 ± 0.6 / 84 ± 0.6 / - / - / tr
VUG-A142c / 15 ± 2 / 84 ± 2 / - / - / tr
VUG-A112 / 94 ± 0.7 / 6.2 ± 0.5 / - / tr / -
aAllstrains were grown on R2A agar at 18°C in the dark for 1 week, except where indicated.
b-: Below detection level
cValues are taken from Klassen and Foght (2008)
dtr: Trace amounts (<1% of the total carotenoids) detected