Central Michigan University
College of Arts & Sciences Master
Course Syllabus
CHM 522 Biochemistry II 3(3-0)
Desig. No. Title Credit (Mode)
I. Bulletin Description:
Continuation of 521. Metabolism of carbohydrates, lipids, amino acids, and nucleotides; molecular biology and genetic engineering.
II. Prerequisites:
CHM 521
III. Rationale for course level:
CHM 522 is a continuation of CHM 521.
IV. Textbooks and other materials to be furnished by the student:
Required textbook: any of several comprehensive biochemistry textbooks may be used. An example is Principles of Biochemistry, 2nd edition, by Lehninger r Nelson, and Cox r Worth Publishers, 1993.
Optional supplement: some publishers provide a student study guide to go with the required textbook.
V. Special requirements for the course:
none
VI. General methodology used in conducting the course:
The course consists of lectures over assigned reading, discussion of subject matter r and homework problems.
VII. Course objectives:
1. Learn both the light and dark reactions of photosynthesis.
2. Learn the metabolism of fatty acids, phosphoglycerides, sphingolipids, terpenes, and steroids.
3. Learn the metabolism of amino acids and nucleotides.
4. Learn the structure of DNA r RNA, and chromosomes.
5. Learn the processes of replication, transcription r and translation.
6. Learn the mechanisms of regulation of gene expression.
7. Learn the steps of recombinant DNA technology.
VIII. Course outline:
SCHEDULE OF LECTURES
Week Subject
1 Photosynthetic pigments
Light reactions and photophosphorylation
2 Photosynthetic reductive pentose cycle
The C-4 pathway and photorespiration
3 Activation and transport of fatty acids
Beta oxidation of fatty acids
Ketone body metabolism
4 Fatty acid biosynthesis
Regulation of fatty acid metabolism
Biosynthesis of terpenes
5 Cholesterol and steroid biosynthesis
Regulation of cholesterol metabolism
Biosynthesis of glycerophospholipids
6 Sphingolipid biosynthesis
Transamination of amino acids
7 The urea cycle
Pathways for catabolism of amino acids
Biosynthesis of nonessential amino acids
8 Biosynthesis of essential amino acids
Biosynthesis of heme and creatine
Regulation of amino acid metabolism
9 Biosynthesis of purine nucleotides
Pyrimidine nucleotide biosynthesis
Salvage, catabolism, and regulation
10 Structure and properties of DNA
Structure and properties of RNA
11 Eukaryotic chromosomes
Replication of DNA
Mutations and DNA repair
12 Transcription of DNA to RNA
Control of transcription in prokaryotes
13 Regulation of eukaryotic gene expression
Processing of eukaryotic hnRNA to mRNA
genetic code
14 Transfer RNA and ribosomes
mRNA translation and protein synthesis
Protein post-translational modification
15 Recombinant DNA technology
Plasmids, cloning, and cDNA libraries
Genetic engineering; PCR
16 Final examination
IX. Evaluation:
Three monthly examinations and a final examination are given. Copies of old examinations are on reserve on the first floor of Park Library. Use them as a practice test or as homework problems. Copies of old final examinations are not on reserve. The grades are determined on a percentage basis. Pluses and minuses are given to those on the upper and lower ends of each grade range.
Grading scale: 90-100% A, 80-89% B, 60-79% C, 50-59% D, 0-49% E
Examinations must be taken during the scheduled time unless prior arrangements have been made to take the examination early. No make-up examinations will be given. An excused absence from one examination will not affect your course grade. An unexcused absence will result in a score of zero for that examination.
The requirements for undergraduates and graduate students are identical.
X. Bibliography:
Textbooks:
1. Biochemistry by Stryer (4th edition), Freeman and Co., 1995
2. Principles of Biochemistry by Horton, et al, Neil Patterson Publishers, 1993
3. Biochemistry by Rawn, Neil Patterson Publishers, 1989
4. Biochemistry by Voet and Voet, (2nd edition), John Wiley & Sons, 1995
5. Biochemistry by Zubay (3rd edition), Wm. C. Brown Publishers, 1993
6. Harper's Biochemistry by Martin, et al (23rd edition), Lange Medical Publications, 1991
7. Biochemistry by Garrett and Grisham, Saunders College Publishing, 1995
8. Biochemistry by Kuchel and Ralston (Shaum's Outline Series), McGraw-Hill Book Company, 1988
9. Biochemistry by Devlin, (3rd edition), John Wiley & Sons, Inc., 1992
10. Biochemistry by Abeles, et al, Jones and Bartlett Publishers, 1992
11. Modern Concepts in Biochemistry by Bohinski, (5th edition), Allyn and Bacon, Inc., 1987
12. Biochemistry by Mathews and van Holde, Benjamin/Cummings Publishing Company, 1990
References:
1. Annual Review of Biochemistry
2. CRC Handbook of Biochemistry
3. Comprehensive Biochemistry
4. Methods in Enzymology
5. Advances in Enzymology
6. Advances in Enzyme Regulation
7. The Enzymes
8. Vitamins and Hormones
9. Advances in Protein Chemistry
10. The Proteins
11. Advances in Lipid Research
12. Progress in Nucleic Acid Research and Molecular Biology
Periodicals:
1. Science
2. Chemical and Engineering News
3. Nature
4. Scientific American
5. Science News
6. Trends in Biological Sciences
Abstracts:
1. Chemical Abstracts
2. Biological Abstracts
Titles:
1. Current Contents
2. Index Medicus
3. Biological and Agricultural Index
4. Chemical Titles
Journals:
1. Journal of Biological Chemistry
2. Proceedings of the National Academy of Science
3. Biochemistry
4. Biochemistry Journal
5. International Journal of Biochemistry
6. Archives of Biochemistry and Biophysics
7. Biochimica et Biophysica Acta
8. Analytical Biochemistry
9. Biochemistry and Biophysics Research Communication
Syllabus Prepared By: Calvin Tormanen
Name
December 1, 1995
Date