A. Human-Dog Top Candidates in Region1 and Region3

Enhancer predication in 8q24 cancer-associated regions. Enhancers were predicted with EEL. Sequences for 8q24 regions and their dog and mouse orthologs were downloaded from UCSC genome website ( Region1 and region3 were predicted by EEL together. Region2 was predicted separately from region1 and region3. The top candidates in both human-dog alignments and human-mouse alignment were compared to each other. The top 4 candidates in region1 and region3, and top 3 candidates in region2 were picked for further analysis. Their positions and ranks are listed in supplementary table. Sequence alignment and predicted transcription factor binding sites are listed below.

A. Human-dog top candidates in region1 and region3:

### lambda=2 mu=0.5 nu=200 xi=200 Nucleotides per rotation=10.4

### D[canFam2_dna][hg18_dna]

Note! First nucleotide at position 1 (one) and binding site at zero!

### Alignment No 1 ###

D[243][109]=44.91 Staf.pfm (4266,4285) <=> (1659,1678) +

D[248][112]=83.77 Androgen.pfm (4309,4330) <=> (1702,1723) +

D[251][114]=128.33 kr.pfm (4342,4351) <=> (1735,1744) -

D[252][115]=168.78 MZF1-4.pfm (4356,4361) <=> (1749,1754) +

D[255][118]=208.67 SOX-9.pfm (4372,4380) <=> (1765,1773) +

D[257][120]=244.57 SPI-B.pfm (4392,4398) <=> (1785,1791) +

D[258][122]=273.38 COUP-TF.pfm (4427,4440) <=> (1820,1833) +

D[261][123]=312.12 AP2alpha.pfm (4455,4463) <=> (1848,1856) +

D[262][126]=343.33 jsmad5.pfm (4480,4484) <=> (1873,1877) +

D[263][127]=376.64 Pax6.pfm (4494,4507) <=> (1887,1900) -

D[267][133]=402.41 jgli.pfm (4524,4532) <=> (1918,1926) +

D[269][135]=446.93 Irf-1.pfm (4535,4546) <=> (1929,1940) +

D[275][141]=463.13 ARNT.pfm (4591,4596) <=> (1985,1990) +

D[277][143]=505.57 E74A.pfm (4601,4607) <=> (1995,2001) +

D[278][144]=544.30 deltaEF1.pfm (4610,4615) <=> (2004,2009) -

D[280][146]=578.54 SPI-B.pfm (4622,4628) <=> (2016,2022) -

D[283][148]=618.25 jCSL.pfm (4660,4670) <=> (2055,2065) -

D[292][154]=544.26 longgli2.pfm (4767,4780) <=> (2171,2184) -

D[296][160]=565.04 Dof2.pfm (4803,4808) <=> (2208,2213) -

D[297][161]=603.24 jsmad5.pfm (4811,4815) <=> (2216,2220) -

D[298][162]=637.29 Broadc2.pfm (4822,4829) <=> (2227,2234) +

D[300][167]=657.64 tcf4.pfm (4873,4881) <=> (2278,2286) -

D[302][170]=689.69 kni.pfm (4896,4905) <=> (2301,2310) -

D[304][173]=730.56 jgci.pfm (4915,4923) <=> (2320,2328) +

D[306][174]=767.66 HMG-IY.pfm (4953,4968) <=> (2358,2373) +

D[308][176]=809.92 Staf.pfm (4973,4992) <=> (2378,2397) -

D[312][180]=853.49 Snail.pfm (5004,5009) <=> (2409,2414) -

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

4257 : tgttttatc-AAATCCCCATCAGCCCAAGActagatcataccaagattttgcaATATGAA

1650 : -gcttcatcaAATCTCCCATCAGCCCAAGActagattgtaccaagattttgcaATATGAA

4316 : CATAATGTTCCCTACtgtccgggaccTAATCCTTTTccatCGGGGAacacatttgcAAAC

1709 : CATAATGTTCTCTACtgtccaggatcTAATCCTTTTccatCGGGGAacacatttgcAAAC

4376 : AATGGctcctaactgaTGAGGAAcaggatccaactcttactccacacaccaGGACCTTTA

1769 : AATGActcctaactgaTGAGGAAcaggatccaactgttactccacaaactaGGACCTTTA

4436 : CACCAcattggaggcaacaGCCCTGAGGttaaagggctgctccgTGTCTaaccactgtAG

1829 : CACCAcattggaggcaataGCCCTGAGGttaaagggccgttccgTGTCTaaccactgaAG

4496 : CTCTTGCTTGAAggaaggtttcccaaa-tGAACCCCCAaaGAAACCGAAAATggcatgaa

1889 : CTCTTGCTTGAAggaaggtttcccaaaatGAACCCCCAaaGAAACTGAAAATggcatgaa

4555 : tgtacatcctgttactcaacactctctgggtttcatAACGTGaagtCAGGAAGaaAAGGT

1949 : cgtacatcctgttactcaacactccccgagtttcatAACGTGaagtCAGGAAGaaAAGGT

4615 : GccaacaTTCCTGAatgttataatcattaaggagcaaactct-aatGTTCCCACACTtga

2009 : GccaacaTTCCTGAatgttattatcattaaggagcaaactcttaatGTTCCCACACTtga

4674 : aagttaaagagatgggat--t--a-t-tcctc---acttatggaatcactgtaaaaccat

2069 : acgttaaagagatgggatcattcagtctcctctttacttatggaattgctgcaaaaccat

4725 : atgggctgaattaggcatccaaca-tgaatgctccctaccattCCCTCTGGGGGGCCtt-

2129 : atgggctgaattaggcatccaaggctaa-tgtttcctaccattCCCTCTGGGGGGCCttt

4783 : ggggctcatcccaggcaattGGCTTTaaAGACAaggaaaTCCTATTTggaaacataatca

2188 : ggggctcatcccaggcacttGGCTTTaaAGACAaggataTCCTATTTggaaacacagtca

4843 : tgagtcctgatattaataagtactttctaaCTTCAAAGCat-tttaataagtgtTGCTCT

2248 : tgagccctgatattaataagcacttcctgaCTTCAAAGCatcttta-taaccatTGCCCT

4902 : AATTaaatctcatAACCTCCCAaagaagag-gcaagcttcatcatttccaccTTGCAAAT

2307 : AATTaaatctcgtAACCTCCCAgaga-gaatgcaggcttcatcatttccactTTACAAAT

4961 : GGAGAAACtgagGCAGAGCCCTATGGAACACAaagccatagtc-CACCTGa--gagtaga

2366 : GGAGAAACtgagGCAGAGCCCTATGGAATATGaaaccgta-tccCACCTGattgagga-a

5018 : ac

2424 : -c

### Alignment No 2 ###

D[4994][6249]=39.21 jsmad5.pfm (72843,72847) <=> (90995,90999) -

D[4996][6251]=84.77 Snail.pfm (72855,72860) <=> (91007,91012) -

D[4997][6252]=121.28 Gfi.pfm (72875,72884) <=> (91027,91036) +

D[4998][6253]=189.20 Myf.pfm (72892,72903) <=> (91044,91055) -

D[5003][6258]=232.30 Thing1-E47.pfm (72905,72914) <=> (91057,91066) +

D[5004][6259]=272.16 Chop-cEBP.pfm (72921,72932) <=> (91073,91084) +

D[5005][6260]=311.52 SOX17.pfm (72941,72949) <=> (91093,91101) +

D[5006][6261]=351.12 SPI-1.pfm (72950,72955) <=> (91102,91107) -

D[5007][6262]=368.83 jsmad5.pfm (72999,73003) <=> (91151,91155) -

D[5008][6263]=412.33 SPI-B.pfm (73006,73012) <=> (91158,91164) -

D[5021][6275]=240.30 HNF-3beta.pfm (73225,73236) <=> (91392,91403) +

D[5026][6277]=230.23 jcETS1.pfm (73308,73316) <=> (91478,91486) -

D[5028][6279]=257.22 MZF1-4.pfm (73338,73343) <=> (91508,91513) -

D[5029][6280]=301.15 E74A.pfm (73345,73351) <=> (91515,91521) +

D[5033][6284]=353.41 Myf.pfm (73355,73366) <=> (91525,91536) -

D[5035][6286]=395.42 Sox-5.pfm (73368,73374) <=> (91538,91544) -

D[5037][6288]=438.42 SPI-B.pfm (73378,73384) <=> (91548,91554) +

D[5040][6290]=463.84 tcf4.pfm (73434,73442) <=> (91604,91612) -

D[5043][6294]=496.89 AP2alpha.pfm (73475,73483) <=> (91645,91653) -

D[5049][6300]=532.64 Dorsal1.pfm (73504,73515) <=> (91674,91685) -

D[5052][6303]=570.91 jgli.pfm (73518,73526) <=> (91688,91696) +

D[5054][6304]=588.61 Dof2.pfm (73565,73570) <=> (91735,91740) +

D[5055][6307]=633.15 SP1.pfm (73580,73589) <=> (91750,91759) +

D[5058][6317]=653.23 USF.pfm (73612,73618) <=> (91781,91787) -

D[5068][6328]=688.57 Myf.pfm (73625,73636) <=> (91793,91804) -

D[5073][6332]=760.83 RORalpha-2.pfm (73637,73650) <=> (91805,91818) -

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

72834 : agtcccaagAGACAttaaattCACCTGtgcttgctctgaaaTAAATCAGTGtccttttCT

90986 : attcccaacAGACActacattCACCTGtgcttgctctgaaaTAAATCAGTGtccctttCT

72894 : GCTGCTGCTGtTGTCTGGAAAtaatgcAAATGCAATGGGcctgcactGACATTGTGCTTC

91046 : GCTGCTGCTGtTGTCTGGAAAtaatgcAAATGCAATGGGcctttactGACATTGTGCTTC

72954 : CCtggaaggatacacataataaattgtcccttaatactgttaaagAGACAttTTCCTCTt

91106 : CCtggaaggatacacataataaattatcccttaatactgttaaagAGACAttTTCCTCTt

73014 : actcaggagcttttgagattggtctgtgctactcacccagcgaggtggaagacatg-gtg

91166 : actcaggagcttttggggttggactgggctactcacccagcaaggaggaggacatgtgt-

73073 : ccttgccactgggtccag-ttgttcatgtgacttcttggtggtctttggc-ctc--c-t-

91225 : c-ttgtcactgg--cccggttattcatgtggcctctcattgctccttggctcactgcatt

73127 : gca---tt------t-c-c--c-agccccccagtt-aagtcataggact--ctagtaacc

91282 : gcaagattcaaggatgcacttccaggcctccacatcaagtcataggacttgccggtaacc

73170 : tagcctggtttccttgtttatgatttgaatttattttattttattgtatgcatgtAACTA

91342 : tagattggttttctcatttgtaatttgaatttattttatgtta---t--gcatttGTATG

73230 : TTTATTT----ggatgcgtga-aagcggaggga-aactggaactcctggtccatgccatt

91397 : TTTATTTattcggatgc-tcagaagctgaag-ataactagtgctcctggtccatgccatt

73284 : cattctttggaagaacgccaaagcTGTTTCCGCagaggacagaaggcattggtcTCCCCT

91455 : catcaattggaagaatgccaa-gcTGTTTCCGCtgaggacagaaggcattggtcTCCCCT

73344 : gCAGGAAGccaCTGCTGCTCCTTaATTGTTTgctAGAGGAAgaatcaagggtaaaattta

91514 : gCAGGAAGccaCTGCTGCTCCTTaATTGTTTgctAGAGGAAgaatcaagggtaaaattta

73404 : aagtaaatggctggctgggttgcactaattCATCAAAGCatgtttcaagtcagtagtcag

91574 : aagtaaatggctggccgagttgcactaattCATCAAAGCatgtttcaagtcagtagtcag

73464 : agcatgcatcaGCCCCTGGCaccaccaccttctatgaaagTGGAAAACCCAGcaGACCTC

91634 : agcatgcatcaGCCCCCGGCgccaccagcttctacgagagTGGAAAAGCCAGcaGACCTC

73524 : CAAgcagatgaaatcattaggaggcattcagtaatgcttgaAAAGCAaagagagagGAGG

91694 : CGAgcagatgaaatcattaggaggcattcagcagggcttgaAAAGCAaagagagagGAGG

73584 : CAGGGAtttctgtgcatgctccactttgCCACATGgggaaaCACCAGTTGTCTGTGACCT

91754 : CGGGGAtttctctgcatgctcc-ctttgCCACATGgg-aaaCACCAGCTGTCTGTGACCT

73644 : AGTTATCtgggagagat

91812 : AGTTATCcaagaaagga

### Alignment No 3 ###

D[839][765]=41.09 RORalpha-1.pfm (13129,13138) <=> (11392,11401) -

D[840][766]=77.70 HLF.pfm (13141,13152) <=> (11404,11415) -

D[843][767]=104.82 cad.pfm (13173,13182) <=> (11436,11445) +

D[848][770]=109.55 jtcf4.pfm (13263,13271) <=> (11526,11534) -

D[849][771]=139.57 hb.pfm (13292,13302) <=> (11555,11565) +

D[851][773]=174.05 SP1.pfm (13328,13337) <=> (11591,11600) +

D[852][775]=200.75 Dof2.pfm (13358,13363) <=> (11621,11626) -

D[854][777]=235.15 jtcf4.pfm (13369,13377) <=> (11632,11640) -

D[855][778]=273.57 Su(H).pfm (13388,13403) <=> (11651,11666) -

D[858][782]=281.62 AP2alpha.pfm (13477,13485) <=> (11740,11748) -

D[860][784]=312.64 Sox-5.pfm (13509,13515) <=> (11772,11778) +

D[864][786]=343.49 c-MYB1.pfm (13531,13538) <=> (11794,11801) -

D[868][788]=390.90 c-MYB1.pfm (13548,13555) <=> (11811,11818) -

D[869][789]=408.00 SPI-1.pfm (13601,13606) <=> (11864,11869) +

D[870][790]=447.04 Thing1-E47.pfm (13607,13616) <=> (11870,11879) +

D[875][794]=403.99 Staf.pfm (13724,13743) <=> (11982,12001) +

D[884][798]=433.17 Broadc1.pfm (13781,13794) <=> (12039,12052) -

D[886][802]=469.24 Su(H).pfm (13803,13818) <=> (12061,12076) -

D[890][808]=441.34 AGL3.pfm (13896,13905) <=> (12158,12167) -

D[900][813]=441.51 AML-1.pfm (13977,13985) <=> (12237,12245) +

D[902][814]=474.74 deltaEF1.pfm (13999,14004) <=> (12259,12264) -

D[916][820]=435.19 jtcf4.pfm (14164,14172) <=> (12424,12432) +

D[917][821]=475.50 MZF1-4.pfm (14178,14183) <=> (12438,12443) -

D[920][829]=497.86 Hen-1.pfm (14249,14260) <=> (12509,12520) +

D[921][831]=542.37 Myf.pfm (14261,14272) <=> (12521,12532) -

D[925][832]=576.21 jCSL.pfm (14283,14293) <=> (12543,12553) -

D[929][837]=601.74 SPI-B.pfm (14313,14319) <=> (12572,12578) -

D[931][839]=622.22 SPI-1.pfm (14329,14334) <=> (12589,12594) +

D[933][841]=669.34 AP2alpha.pfm (14338,14346) <=> (12598,12606) +

D[939][847]=692.90 Snail.pfm (14398,14403) <=> (12658,12663) +

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

13120 : ccaatcgtgTGACCCCGGTcaAATTGCTTAACCtctccctatctcaggtccccATCCATA

11383 : ccaattgccTGACCCTGGTcaAATTGCTTAACCtcttcctatctcagctccctATCCATA

13180 : AAAcagagggacgagcaaactctccacctaccactatggggtgtagccagagttaatacc

11443 : AAAcagagggacgaataaactctcctcctaccactaagaggtgtagccagagttaatacc

13240 : ctcatcgtcctttgagctcagcaGATGAAAGGcactgaaaaaagtacaaagaATTTTTAT

11503 : ctcatcgtcctttgagctcagcaGATGAAAGGcactgagaaaagtacaaagaATTTTTAT

13300 : GTGctattgactttattttattttctgtGGGGGAGGGAgtctgccccagctggaaagcTG

11563 : GTGctattgactttattttattttatgtGGGGGAGGGAgccggccccagctggaaagcTG

13360 : CTTTctctgAATCAAAGGgcaggaacccAGCAAGTTTCTCAGGAtcagggcctcagactg

11623 : CTTTctctgAATCAAAGGgcaggaacccAGCAAGTTTCTCAGGAttggggccttagactg

13420 : agccgtgtacactcacggtcccagccaatcct-ccagttcagtttcctttaacctggtGC

11683 : ggctgtgtatacagacagtgccagccaaccccaca-gttcagtttcctttaacctggtGC

13479 : TCCAGGCaataactgtgcaactctgcaattTAACAATgtgttctttgtctcaCAACTGTT

11742 : TCCAGGCaataactgtgcaactctgcaattTAACAATgtgttctttgtcccaCAACTGTT

13539 : ctcatttcgCAACTGCCcaggtaataggtttgagcctgtaggaagagtcaaatagttaat

11802 : ctcgtttctCAACTGCCcaggtaatatgtttgggcctgtaggaagagtcaaatagttaat

13599 : gaGGGAAGGGTTTGGCATgccctac--agaggttctatccgcaa-gtctcaacaagaagg

11862 : aaGGGAAGGGTTTGGCATgccctacgta-ag-ttcta-ccagcaagtcccaacaagaagg

13656 : cattctctctttcctgggttcc-gacctacccccagaatgtacaagtgtcactaccaaga

11919 : cattctgtgtctcctga-ttcctgacctacccccaaaatgtacaaatgt-ac-a--agga

13715 : atgagccttCTTTTCCCAGCAGGCTGTAAtaccagtttggcttttatcaatgcattggta

11974 : atgagccc-ACTTTCCCAGCAGGCTGTAAtaccagtttggcctatatcaatgcattggtg

13775 : aggtgtGTTTTGTTTATGGTtttatgccGTCTATTTTCCCAGGGatattatgttttgtac

12033 : agctgtGTTTTGTTTATGGTtttatgccATCTATTTTCCCATGGatattatgttttctaa

13835 : agagccctggagtttgcgtcagcttttaaagctac--g--gcaccttttcagttcttatt

12093 : agagcccttaagtttacgtcagcttttaaagctaccagcagcaccatttcagttcatatt

13891 : aagccCTTAATATGGtatgaataggggagctattagacaagaaaaagccataatcgcccc

12153 : aagccCTTAATATGGtatgaataggagagctattagacta-aaga-gccataatcatccc

13951 : caaggaaaacatccatcaccagccttTATGTGGTCcctgaacctctaaAAGGTGttgtct

12211 : tgaggaaaacatccatcaccaacattTATGTGGTCcctgaacttctaaAAGGTGtcatct

14011 : ctctggggtgtatctggtgagag-tgttctctgg-a-aatgccaagaaacc-agtgcatt

12271 : ctctggggtgtatctggtgagagct-ttctctgggaga-tgccaa-aaagccaatgcatt

14067 : cgatggtatcttagaaacgcattttctaaacattacaaattgtcttgtttagtgtctcaa

12328 : agatga-agcttagaagggcattttctaaccattacaaattgcctagtctagcatctcaa

14127 : tttcatctatgggatgagcctcaattaactttgtcagAGTTTGATCctttgTCCCCAgat

12387 : tttcatctacgtgaagagccttaattaaatttgttggGGTTTGATCctttaTCCCCAgat

14187 : gtggctctgacaaagattgctgacataaataatatgggctccaagcccttgccaggctcc

12447 : gtggcgctgacagagattgcttacataaataatgtgtgctccaagtgcttgccaggctcc

14247 : agGCTCAGCTGGGACAGCTGTGGCTTtttaaatgtcATTCCCAAGATatcctggaggtgt

12507 : tgGCTCAGCTGGGACAGCTGTAGCTTtttgaatgtcATTCCCAAGATatcctgcaggtgt

14307 : tcggctTTCCTCT-ttctaatctGGGAAGggaGCCGTGGGCaacatcagcccagatgaca

12567 : tcagc-TTCCCCTgttctactctGGGAAGagaGCCGTGGGCaacatcagcccagaagaca

14366 : ctaatgcatcctgacagagcattcctgctgtaCAGGTGcgctacctgc

12626 : ctagtgcatcttgacagagcattcccactggaCAGGTGcactacctgc

### Alignment No 4 ###

D[3040][3594]=42.81 MZF1-4.pfm (44204,44209) <=> (52763,52768) -

D[3041][3595]=79.23 Gfi.pfm (44215,44224) <=> (52774,52783) -

D[3044][3597]=113.39 NRF-2.pfm (44241,44250) <=> (52800,52809) -

D[3045][3599]=141.87 Gfi.pfm (44268,44277) <=> (52827,52836) -

D[3048][3601]=172.67 Irf-1.pfm (44295,44306) <=> (52854,52865) +

D[3050][3603]=211.38 jsmad5.pfm (44308,44312) <=> (52867,52871) -

D[3051][3604]=246.64 deltaEF1.pfm (44325,44330) <=> (52884,52889) +

D[3056][3610]=275.95 jCSL.pfm (44361,44371) <=> (52920,52930) -

D[3058][3613]=298.06 deltaEF1.pfm (44384,44389) <=> (52942,52947) +

D[3064][3618]=344.08 Dorsal2.pfm (44415,44424) <=> (52973,52982) -

D[3069][3620]=358.78 Dof2.pfm (44469,44474) <=> (53027,53032) -

D[3071][3625]=378.70 Broadc1.pfm (44492,44505) <=> (53051,53064) +

D[3077][3627]=424.01 NF-kB.pfm (44510,44519) <=> (53069,53078) +

D[3082][3628]=455.11 hb.pfm (44535,44545) <=> (53094,53104) -

D[3083][3629]=491.14 HLF.pfm (44548,44559) <=> (53107,53118) +

D[3085][3631]=511.96 Nkx.pfm (44591,44597) <=> (53150,53156) +

D[3087][3632]=534.53 HNF-3beta.pfm (44632,44643) <=> (53191,53202) -

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

44195 : attctgatgTCCCCAattcaGAGAGATTTCctggaaacagagccagCCCTTCCAGTaagc

52754 : attctgatgTCCCCAtttcaGAGAGATTTCtttgaaacagagccagCCCTTCCAGTaagc

44255 : gtatataacagagTAGTGATTGCagagagtgg-gccctgatGGAAACAAAACCcAGACAt

52814 : atatataacagaaTGGTGATTTCagagagtggagcc-tggtGGAAACAAAACCcAGACAt

44314 : gtaaaatttgaCACCTGaaccgacaggagtgcccatgctccctcccaATTCCCACTCCct

52873 : gtaaaatttgaCACCTTaaccgacataagtgcccacattccctgcgtTTTCCCACTCTct

44374 : gct-ccactccCACCTTcaacgtggccataattttatt-tgggGGAAAACCACaaccagc

52933 : gcctc-ac-ccCACCTTcaacatgcccataattttattctga-GGAAAACCACaaccagc

44432 : acttaacaccaggag-ccaaattccctggcagcaaccaTGCTTTtaaaagggagagatga

52990 : acttaccaccag-agtccaaattccctggcagcagcctTGCTTTtaaaagggagacataa

44491 : a-AAAATTGTCAAGACctatGGGGATTTCTaacacattaggagggCCCCCAAAAACtgTG

53049 : aaAAAATTGTCAAGACctatGGGGCTTCCTaacacattaaaaaggCCCTCAAAAACtgTG

44550 : TTACACCAAGcataatattttatttttctttaagagctttcTTAATTGgttccatgcaaa

53109 : TTACACCAAGcataatattatctttttctttaagagatttcTTAATTGgttccatgcaaa

44610 : aggcttgcaggtaatgagttttAAATAAATAATAat-tatgttcc

53169 : aggtttgtaggtaatgggttttAAATAAATAATAatgt-tccacc

B. Human-mouse top candidates in region1 and region3:

### D[mm9_dna][hg18_dna]

Note! First nucleotide at position 1 (one) and binding site at zero!

### Alignment No 1 ###

D[458][109]=45.56 Staf.pfm (6954,6973) <=> (1659,1678) +

D[462][112]=73.61 Androgen.pfm (6996,7017) <=> (1702,1723) +

D[464][114]=118.17 kr.pfm (7029,7038) <=> (1735,1744) -

D[465][115]=158.80 MZF1-4.pfm (7043,7048) <=> (1749,1754) +

D[467][117]=196.32 Sox-5.pfm (7059,7065) <=> (1765,1771) +

D[470][120]=231.22 SPI-B.pfm (7079,7085) <=> (1785,1791) +

D[472][123]=211.18 AP2alpha.pfm (7138,7146) <=> (1848,1856) +

D[476][124]=218.98 c-REL.pfm (7151,7160) <=> (1862,1871) +

D[480][128]=242.29 jSTAT1.pfm (7197,7204) <=> (1908,1915) -

D[485][133]=283.59 jgli.pfm (7207,7215) <=> (1918,1926) +

D[487][135]=323.13 Irf-1.pfm (7218,7229) <=> (1929,1940) +

D[493][137]=361.04 Androgen.pfm (7235,7256) <=> (1946,1967) +

D[497][143]=387.07 E74A.pfm (7283,7289) <=> (1995,2001) +

D[499][144]=425.80 deltaEF1.pfm (7292,7297) <=> (2004,2009) -

D[500][146]=460.05 SPI-B.pfm (7304,7310) <=> (2016,2022) -

D[501][147]=507.26 Su(H).pfm (7337,7352) <=> (2049,2064) -

D[504][149]=519.08 Tal1beta-E47S.pfm (7409,7420) <=> (2122,2133) +

D[509][160]=518.57 Dof2.pfm (7494,7499) <=> (2208,2213) -

D[510][162]=549.11 Broadc2.pfm (7513,7520) <=> (2227,2234) +

D[511][163]=583.33 TCF11-MafG.pfm (7530,7535) <=> (2244,2249) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

6945 : tggctt-atc-AAATCCCCATCAGCCCTTAC-tagatcatatcaagattttgcaATATGA

1650 : -g-cttcatcaAATCTCCCATCAGCCCAAGActagattgtaccaagattttgcaATATGA

7002 : ACATAATGTTCTCTACtgtccaggaccTAATCCTTTTtcatTGGGGAgcacatttgcAAA

1708 : ACATAATGTTCTCTACtgtccaggatcTAATCCTTTTccatCGGGGAacacatttgcAAA

7062 : CAATgactcctaactcaTGAGGAAcagcatccaactcttactccac---c-aggaccttt

1768 : CAATgactcctaactgaTGAGGAAcaggatccaactgttactccacaaactaggaccttt

7118 : agtccacgttggaggcagcaGCCCCGAGGttaa-AGGGCTTTCCtgagtctaaca--ttg

1828 : acaccacattggaggcaataGCCCTGAGGttaaaGGGCCGTTCC-gtgtctaaccact-g

7175 : aagctctatctt-gaaagaagatTTCCCAAAatGAACCCCCAaaGAAACCAAAAACagca

1886 : aagctct-tgcttgaaggaaggtTTCCCAAAatGAACCCCCAaaGAAACTGAAAATggca

7234 : tGAACGTACATCCTGTTACTCAAcacgccctg-gtttcctaatgcaaagtCAGGAAGagA

1945 : tGAACGTACATCCTGTTACTCAAcactccccgagtttcataacgtgaagtCAGGAAGaaA

7293 : AGGTGccaacaTTCCTGAatgttagaatcatcacag-agcaaactCGTACTGTTCCCACA

2005 : AGGTGccaacaTTCCTGAatgttattatcatta-aggagcaaactCTTAATGTTCCCACA

7352 : Ctcgaacactaaca-agctagacttc-ttgc-tctcctctttacttatggaatct-ctgc

2064 : Cttgaacgttaa-agagatggg-atcattcagtctcctctttacttatggaat-tgctgc

7408 : aAAACCATATGGGcc-atgttaggcatc-a-ggc-actaactctccctatcattctctct

2121 : aAAACCATATGGGctgaa-ttaggcatccaaggctaat--gt-ttcctaccattccctct

7464 : aaggga--tttgcagactcattccaggcaagtGGCTTTaaa-agctaggttgTCCTATTT

2177 : ggggggcctttgggg-ctcatcccaggcacttGGCTTTaaaga-caaggataTCCTATTT

7521 : ggaaatataGTCATGggccctgaca

2235 : ggaaacacaGTCATGagccctgata

### Alignment No 2 ###

D[6111][6246]=53.62 kr.pfm (88686,88695) <=> (90961,90970) +

D[6113][6251]=84.69 Snail.pfm (88732,88737) <=> (91007,91012) -

D[6114][6252]=121.19 Gfi.pfm (88752,88761) <=> (91027,91036) +

D[6117][6257]=162.30 Myf.pfm (88774,88785) <=> (91050,91061) -

D[6120][6261]=178.69 SPI-1.pfm (88825,88830) <=> (91102,91107) -

D[6121][6262]=196.40 jsmad5.pfm (88874,88878) <=> (91151,91155) -

D[6122][6263]=239.89 SPI-B.pfm (88881,88887) <=> (91158,91164) -

D[6125][6266]=225.60 Pax-2.pfm (88947,88954) <=> (91227,91234) +

D[6136][6275]=174.18 HNF-3beta.pfm (89108,89119) <=> (91392,91403) +

D[6140][6279]=79.79 MZF1-4.pfm (89215,89220) <=> (91508,91513) -

D[6141][6280]=123.73 E74A.pfm (89222,89228) <=> (91515,91521) +

D[6145][6284]=175.98 Myf.pfm (89232,89243) <=> (91525,91536) -

D[6147][6286]=218.00 Sox-5.pfm (89245,89251) <=> (91538,91544) -

D[6152][6290]=238.42 tcf4.pfm (89311,89319) <=> (91604,91612) -

D[6157][6294]=269.73 AP2alpha.pfm (89352,89360) <=> (91645,91653) -

D[6163][6317]=208.78 USF.pfm (89494,89500) <=> (91781,91787) -

D[6167][6322]=251.59 c-REL.pfm (89501,89510) <=> (91788,91797) -

D[6170][6327]=289.79 jsmad5.pfm (89513,89517) <=> (91800,91804) +

D[6176][6332]=362.05 RORalpha-2.pfm (89518,89531) <=> (91805,91818) -

D[6181][6337]=398.73 Bsap.pfm (89540,89559) <=> (91827,91846) +

D[6190][6341]=424.44 Staf.pfm (89593,89612) <=> (91880,91899) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

88677 : aga-acctcgGAATGGGTTAagtcctctctggctcggtcccaagaggcattaaattCACC

90952 : aaagac-tcaGAACGGGTTAagtgctccctggctcattcccaacagacactacattCACC

88736 : TGtacttgctctgaaaTAAATCAGTGtccctttctgct-CTGCTGTTGTCTggaaataat

91011 : TGtgcttgctctgaaaTAAATCAGTGtccctttctgctgCTGCTGTTGTCTggaaataat

88795 : gcaaatgtca--gggtctgcactgtctttgtgCTTCCCtggaaggatgcgtataataaat

91071 : gcaaatg-caatgggcctttactgacattgtgCTTCCCtggaaggatacacataataaat

88853 : tgtcccttaatactgttaaagAGACAttTTCCTCTtactcaggagcttttgaggttggtc

91130 : tatcccttaatactgttaaagAGACAttTTCCTCTtactcaggagcttttggggttggac

88913 : -gcagctactcactcagc--g-aggagcacatgcgtctTGTCACTGggcccacttactcc

91190 : tgg-gctactcacccagcaaggaggaggacatgtgtctTGTCACTGgcccgg-ttattca

88969 : tgtggcctctcattgctccttggctc-ctcgttttattaag-ttcaaggatatgttct-c

91248 : tgtggcctctcattgctccttggctcact-gcattgc-aagattcaaggat--gcacttc

89026 : cag-cctctttgttgaggcg-agggagttgctggtaatcaagatgggtttccttgtttgt

91304 : caggcctccacatcaagtcatagg-acttgccggtaacctagattggttttctcatttgt

89084 : gctttgaatttgtttcacgt---g--tttATATGTTTATTTattcggatgtttggaagct

91363 : aatttgaatttattttatgttatgcatttGTATGTTTATTTattcggatgctcagaagct

89139 : -aagggaaa-a-tggtcc-g-t----gccattcatcatttggaagaatgccaagcggttt

91423 : gaagataactagtgctcctggtccatgccattcatcaattggaagaatgccaagctgttt

89190 : ccacagaggacagaaggcattggtcTCCCCTgCAGGAAGccaCTGCTGCTCCTTaATTGT

91483 : ccgctgaggacagaaggcattggtcTCCCCTgCAGGAAGccaCTGCTGCTCCTTaATTGT

89250 : TTgctggaggaagaatcaagggtaaaatttaaagtaaatggctggcc-atcttgcgctaa

91543 : TTgctagaggaagaatcaagggtaaaatttaaagtaaatggctggccgag-ttgcactaa

89309 : ttCATCAAAGCgtgtttcgagtcagtagtcagagcatgcctcaGCCTCTGGCgccaccaa

91602 : ttCATCAAAGCatgtttcaagtcagtagtcagagcatgcatcaGCCCCCGGCgccaccag

89369 : cttccatgagagtggaaacatta-gcggagctcagagcagacgagatccttaggaggcat

91662 : cttctacgagagtggaaa-agccagcagacctccgagcagatgaaatcattaggaggcat

89428 : tcagc-ggtgcttgaaaagcaaag-gaagaagaggcggcgatttctatgcatgctagtgc

91721 : tcagcagg-gcttgaaaagcaaagaga-gaggaggcggggatttctctgcatgct----c

89486 : tccgtttgCCACATGGGAAACACCAgtTGTCTGTGACCTAGTTATC-agagagagGAAGC

91775 : -cc-tttgCCACATGGGAAACACCAgcTGTCTGTGACCTAGTTATCca-agaaagGAAAC

89545 : AGGGAAGAAAACCCAcaaagctgtttgctgtgtgggaatctcagacttCCGAGGCACCCT

91832 : ACGGAAGAGAACCCAcaaaactgtttgctacatgagaaccccattctcCAAAGACATGCT

89605 : GCGTGCTGagaaaataat

91892 : GGATGTTGagaaaacaat

### Alignment No 3 ###

D[1179][770]=44.73 jtcf4.pfm (17668,17676) <=> (11526,11534) -

D[1180][771]=74.75 hb.pfm (17697,17707) <=> (11555,11565) +

D[1181][772]=103.47 MZF1-4.pfm (17733,17738) <=> (11591,11596) +

D[1182][775]=128.17 Dof2.pfm (17763,17768) <=> (11621,11626) -

D[1184][776]=162.57 tcf4.pfm (17774,17782) <=> (11632,11640) -

D[1185][778]=200.99 Su(H).pfm (17793,17808) <=> (11651,11666) -

D[1186][779]=237.88 SP1.pfm (17823,17832) <=> (11681,11690) +

D[1188][781]=259.96 SP1.pfm (17846,17855) <=> (11705,11714) -

D[1189][782]=286.69 AP2alpha.pfm (17880,17888) <=> (11740,11748) -

D[1191][784]=317.71 Sox-5.pfm (17912,17918) <=> (11772,11778) +

D[1194][786]=348.55 c-MYB1.pfm (17934,17941) <=> (11794,11801) -

D[1197][789]=357.15 SPI-1.pfm (18004,18009) <=> (11864,11869) +

Sequence 1: mm9_dna

Sequence 2: hg18_dna

17659 : agctccgcaGATGAAAGGcactgagaaaagtacaaagtATTTTTATGTGctattgacttt

11517 : agctcagcaGATGAAAGGcactgagaaaagtacaaagaATTTTTATGTGctattgacttt

17719 : attttattttatgtGGGGGAggaagtttgccccagctggaaagtTGCTTTctctgAATCA

11577 : attttattttatgtGGGGGAgggagccggccccagctggaaagcTGCTTTctctgAATCA

17779 : AAGGgcaggaacccAGCAAGTTTCTCAGGAtcaggaccttaggcTGGGCTGTGTgtacag

11637 : AAGGgcaggaacccAGCAAGTTTCTCAGGAttggggccttagacTGGGCTGTGTatacag

17839 : a--gtgccaACCAACCCCA-cagttcagtttcctttaacctggtGCTCCAGGCaataact

11697 : acagtgcc-AGCCAACCCCacagttcagtttcctttaacctggtGCTCCAGGCaataact

17896 : gtgcaactctgcaattTAACAATgtgttcgttgtcccaCAACTGTTcttgtttcgcaatt

11756 : gtgcaactctgcaattTAACAATgtgttctttgtcccaCAACTGTTctcgtttctcaact

17956 : gcccaggtaatatgtttgggcctgtaggaagagtcaaatagttaatgaGGGAAGggcttg

11816 : gcccaggtaatatgtttgggcctgtaggaagagtcaaatagttaataaGGGAAGggtttg

18016 : gcat

11876 : gcat

### Alignment No 4 ###

D[9315][9500]=42.48 TEF-1.pfm (134142,134153) <=> (136599,136610) +

D[9319][9501]=79.54 cad.pfm (134161,134170) <=> (136618,136627) +

D[9322][9503]=116.51 jCSL.pfm (134185,134195) <=> (136642,136652) -

D[9324][9505]=159.70 jtcf4.pfm (134214,134222) <=> (136671,136679) +

D[9326][9508]=193.41 jsmad5.pfm (134234,134238) <=> (136691,136695) -

D[9329][9514]=221.40 Thing1-E47.pfm (134284,134293) <=> (136741,136750) -

D[9333][9518]=253.56 Elk-1.pfm (134322,134331) <=> (136778,136787) -

D[9336][9520]=296.81 c-FOS.pfm (134339,134346) <=> (136795,136802) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

134133 : cttaa-caaaTCCATTCCTCAGtttccccAGCCATAAAAcagtgataatcataGTTCCCA

136590 : cttgggcaa-GCCATTCCTCAGtttctccAGCCATAAAAtagggataatcataGTTCCCA

134192 : CCAGagatactctttgtcaggaCCTTTGATGttagtttccacAGACAaagggctgtttta

136649 : CCAGagatactcttcatcaggaCCTTTGATGtcagtttccacAGACAaagggctggtata

134252 : attattctgag-tgcagtaatgaggaaggcaccATGCCAGACTgacttttataagacttt

136709 : attatccc-aggtgcagtaatgagaaaggcagaAAGCCAGACTgttttttataggacttc

134311 : tcaagtaccagTTTCCGGCTGctg-ccaaTGAGTCACattggacca--c

136768 : tcaggtag-agTTTCCGGCTTctgtcca-TGAGTCATat-ggacaatg-

### Alignment No 5 ###

D[6900][6988]=42.42 Hunchback.pfm (100611,100620) <=> (100667,100676) -

D[6902][6990]=79.08 Broadc4.pfm (100631,100641) <=> (100687,100697) +

D[6905][6991]=120.96 jCSL.pfm (100653,100663) <=> (100709,100719) +

D[6906][6992]=167.66 Athb-1.pfm (100670,100677) <=> (100726,100733) +

D[6914][6996]=181.58 SOX-9.pfm (100727,100735) <=> (100783,100791) +

Sequence 1: mm9_dna

Sequence 2: hg18_dna

100602 : a-tcacacacTTTTTTTTGCaataataatgTAGTAAGTAAAtacatgcttacTCCATGGG

100658 : agtcacaga-CTTTTTTTTCagtaataatgTAGTAAATAAAtacatgctcacTCCATGGG

100661 : AAAtggtggCAATTATTgagaacacatgttcac-ctcaccatattgctcactgataattg

100717 : AAAtggtggCAATTATTaagagcacacattcacac-catcatattgcttactgataactg

100720 : tgcaattAAACAATGActgtgtgcta

100776 : tgcagttAACCAATGGcagtgtgcta

### Alignment No 6 ###

D[3498][3595]=41.11 Gfi.pfm (52024,52033) <=> (52774,52783) -

D[3500][3597]=75.27 NRF-2.pfm (52050,52059) <=> (52800,52809) -

D[3502][3601]=100.22 Irf-1.pfm (52104,52115) <=> (52854,52865) +

D[3505][3603]=138.92 jsmad5.pfm (52117,52121) <=> (52867,52871) -

D[3506][3604]=172.66 deltaEF1.pfm (52134,52139) <=> (52884,52889) +

Sequence 1: mm9_dna

Sequence 2: hg18_dna

52015 : tccccattt--CAGAGATTTCtttgaaacattgccagCCCTTCCAGTaagcatatagcat

52765 : -ccc-atttcaGAGAGATTTCtttgaaacagagccagCCCTTCCAGTaagcatatataac

52073 : agaatggtgatttttcagagtgcagtctggtGGAAATGAAACCcAGACAtgtaaaatttg

52823 : agaatggtgatttcagagagtggagcctggtGGAAACAAAACCcAGACAtgtaaaatttg

52133 : aCACCTTaa--gtacccac

52883 : aCACCTTaaccg-acata-

C. Human-dog top candidates in region2:

### lambda=2 mu=0.5 nu=200 xi=200 Nucleotides per rotation=10.4

### D[canFam2_dna][hg18_dna]

Note! First nucleotide at position 1 (one) and binding site at zero!

### Alignment No 1 ###

D[3858][4319]=39.60 SPI-1.pfm (54631,54636) <=> (63560,63565) -

D[3860][4321]=82.80 Pax-4.pfm (54639,54668) <=> (63568,63597) +

D[3861][4323]=118.60 Elk-1.pfm (54670,54679) <=> (63599,63608) -

D[3862][4324]=170.11 COUP-TF.pfm (54684,54697) <=> (63613,63626) +

D[3866][4328]=192.44 MZF5-13.pfm (54725,54734) <=> (63653,63662) -

D[3869][4331]=220.13 tcf4.pfm (54754,54762) <=> (63683,63691) -

D[3871][4333]=253.39 Broadc1.pfm (54790,54803) <=> (63719,63732) +

D[3874][4335]=270.13 Su(H).pfm (54861,54876) <=> (63789,63804) +

D[3880][4344]=278.81 MZF1-4.pfm (54907,54912) <=> (63833,63838) -

D[3889][4350]=259.59 Athb-1.pfm (55015,55022) <=> (63943,63950) +

D[3892][4351]=286.19 SPI-1.pfm (55049,55054) <=> (63977,63982) +

D[3896][4355]=293.73 Dorsal2.pfm (55116,55125) <=> (64044,64053) +

D[3897][4359]=310.55 E2F.pfm (55157,55164) <=> (64084,64091) -

D[3898][4360]=349.76 jsmad5.pfm (55165,55169) <=> (64092,64096) -

D[3901][4361]=373.20 tcf4.pfm (55198,55206) <=> (64125,64133) -

D[3905][4365]=394.83 jtcf4.pfm (55252,55260) <=> (64179,64187) +

D[3907][4367]=414.22 TBP.pfm (55295,55310) <=> (64222,64237) +

D[3909][4369]=460.17 HMG-IY.pfm (55318,55333) <=> (64245,64260) +

D[3915][4377]=472.49 HLF.pfm (55398,55409) <=> (64324,64335) -

D[3919][4381]=484.57 Broadc1.pfm (55465,55478) <=> (64391,64404) -

D[3925][4385]=487.98 MZF1-4.pfm (55553,55558) <=> (64478,64483) +

D[3928][4393]=491.85 Evi-1.pfm (55630,55643) <=> (64555,64568) -

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

54622 : gtctggactCTTCCCtgGAATCATATCCTCTTGTGGCACCTCCCTTCtCTTCAGGATGct

63551 : gcctggagtCTTCCCtgGAAACATGTTCCCTTATCGCACCTCCCTTCtCTTCAGGATGct

54682 : gaGGACCTCTGGCCTCcatggacttcatcctcctgtacaattgGTTCCCCTCAcaat-ag

63611 : gaGGACCTCTGGCTCC-atggacttcatcctcccatacaattgGTTCCCCTCAcaattag

54741 : ttcctggcacaatTATCAAAGGgagctctctccaaggacgtggaacaggATGAAAAACAA

63670 : tttctggcactgtCATCAAAGTgatccctctccaaggacatggaacaggATGAAAAACAA

54801 : ATCatatattgtaaactcaaagtgcaatcaagacaagctcagcaaaggaggaagcttatg

63730 : ATTgta-attgtaaactcgaagtacgatcagggcaggcacagcaaaggaggaaggttacg

54861 : TTGTGGGAATTGGGAGtcaacagggaaagacagcc--catcgcctgccTCCCCC--aggt

63789 : TTGTGGGAATCAGGGGtcaacaataaaagccagcctgc-t-gcctg--TCCCCAccaggc

54917 : aatttg-tgggagaaggcatatg-ccc-atttatgcctg-aaacatgcccagggcag-ag

63845 : aattttctggg--c-tgttcatgtccccagttatgcccccaaacattcccatgacagcaa

54972 : aatgcttaaggaacagaatctctgaagattttacc-aaaacttaGAATCATTtttctgtc

63902 : --tgcctaaggaaaataacctctgaagattgagcctaaaacta-GAATCATTtttctctc

55031 : ttgttccgaat-catagcaGGGAAGcagactgtttcctcacatccagaaagtatcaggct

63959 : tcattct-aattcatagcaGGGAAGtggactggctccttaaatccagaaagtatcagact

55090 : tatcccttaaaatccttagggcaagtGTGGTTTGCCctcatcttctgacagcatggatga

64018 : tatctgatcaaatcattagggcaactGTGTTTTCCC-tcatctccagacagcatggatga

55150 : agaaaagGAGCCAAAAGACAcagcatctctcttccaatgaaaactcagCTTCAAAGAaga

64077 : ggagaagGAGCCAAAAGACAcagcatctctcttcaaatgaatactcaaCTTCAAAGAaga

55210 : aactgaccgttgttgaggaggtgaagtcacagccttggcaaaCCTTTGAAGctggaggag

64137 : aagtgaccactgttaaggaggtgaagtcatagcctcggcaaaCCTTTGAAGctgggggag

55270 : aagatgtaaattaagcgcagacaagCAGGGTATAAAAATAAaatccttGAAGGAAAAGCA

64197 : aagatgtaaattaagcaccgacaagCAGGGTATAAAAATAAaatccttGAAGGAAAAGCA

55330 : AAACt-catctgcaatgggtgtgaatgtaaaggaagttggccaaggcaaggaggagataa

64257 : AAACtgctt-tgcaatgggtgtgaaagtaaaggaagctggccaaggccag-aggaggtaa

55389 : acaggattgCATGATGTAATCacactatgaaaaggcaaattagatgagaaatata-agga

64315 : acagggttgCATGATGTAACCacactaggaaaaggcaaactagttgagaaatatagag-a

55448 : aaatacatggaagctgcAAATTGTCTATTTTatctgagaacttgtaagaatcaaagtgaa

64374 : aaatacatggaagttgtAAATTGTCTATTTTatctgagaacttataagaatcaaagtgga

55508 : agcaatgtcaattttctcaatattcagttcattataaaccc--gcttGGGGGA-caagaa

64434 : aacaaggtcaattttcttaatattcaggccattataaacccttg---TGGGGAacaggaa

55565 : acatgattttatttgctgtttttgcaatagtattactctggtgta-a-gacaaggacg-t

64491 : acttgcttttatttgctgtttctgcaatagtgttgtt-tgg-gtacacgaca-ggaccat

55622 : ggggctctTATAATCTTGTGTAaggctccatg

64548 : ggaactc-TATAATCTTGTATAgggatacatg

### Alignment No 2 ###

D[3290][3622]=59.71 Su(H).pfm (46702,46717) <=> (53738,53753) +

D[3292][3624]=86.29 SAP-1.pfm (46743,46751) <=> (53779,53787) -

D[3293][3626]=125.72 Sox-5.pfm (46755,46761) <=> (53791,53797) -

D[3297][3629]=156.02 Pbx.pfm (46780,46791) <=> (53816,53827) -

D[3300][3631]=172.46 cEBP.pfm (46829,46840) <=> (53866,53877) +

D[3302][3632]=202.36 jgci.pfm (46860,46868) <=> (53897,53905) -

D[3305][3635]=238.37 GAMYB.pfm (46881,46890) <=> (53918,53927) +

D[3308][3636]=271.74 FREAC-4.pfm (46899,46906) <=> (53936,53943) +

D[3311][3638]=310.19 HNF-3beta.pfm (46914,46925) <=> (53951,53962) +

D[3312][3639]=359.40 Athb-1.pfm (46927,46934) <=> (53964,53971) +

D[3313][3640]=403.55 Myf.pfm (46938,46949) <=> (53975,53986) +

D[3315][3641]=421.48 Thing1-E47.pfm (47008,47017) <=> (54045,54054) -

D[3316][3642]=448.68 Dof2.pfm (47037,47042) <=> (54074,54079) +

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

46693 : ctaatgtccCTGTGGGAGCCCTTCTtgcttagattgtcgccggcagatttACTTCCAGTa

53729 : ctaatgtccCTGTGAGAACCCATCTtgctgagattgtcgctggcagatttACTTCCAGTg

46753 : tgATTGTTGcaagaatttgtttaacagAATGAATGATCAaccctgagtaaaag-gattat

53789 : tgATTGTTGcaagaatttgtctaacagAATGAATGATCAaccttgagcagaagagattat

46812 : gaaaaacttaatagcatTGTAGCAATGTGgttgttaatgaaatacagtTGGCTGCTCccg

53849 : gaaaaacttaatagcatTGTAGCAATGTGgctgttaatgaaatacagtTGGCTGCTCccg

46872 : ccatttggcACCAACCGACccaacactGTCAACATcacaataCGATATTTGTTCcCAATT

53909 : ctgtttggcACCAACCAACctgacactGTCAACATcacaataCGATATTTATTCcCAATT

46932 : ATTttaCAGCAACAACTGaaatacaatctggtattaatcatatttattataagtatcaat

53969 : ATTttaCGGCAACAACTGaaatacaatgtgttattaatcatatttattataagtatcaat

46992 : ttgagaaatttctgacAAGCCAGAAGatgaataggtttattatgaAAAGCAattctgctt

54029 : ttgagaaatttctgacATGCCAGAAGataaataggtttattatgaAAAGCAgttctgctt

47052 : g

54089 : g

### Alignment No 3 ###

D[5342][6487]=37.37 FREAC-4.pfm (77235,77242) <=> (96397,96404) +

D[5346][6491]=60.38 SP1.pfm (77261,77270) <=> (96422,96431) -

D[5351][6495]=80.48 Tal1beta-E47S.pfm (77301,77312) <=> (96463,96474) -

D[5354][6496]=113.74 Pax-2.pfm (77319,77326) <=> (96481,96488) +

D[5355][6497]=138.59 Sox-5.pfm (77356,77362) <=> (96518,96524) -

D[5357][6501]=196.69 RORalpha-2.pfm (77374,77387) <=> (96536,96549) -

D[5360][6504]=226.82 AP2alpha.pfm (77405,77413) <=> (96567,96575) +

D[5363][6507]=267.19 NF-kB.pfm (77417,77426) <=> (96579,96588) -

D[5366][6509]=299.53 SPI-B.pfm (77443,77449) <=> (96605,96611) -

D[5369][6514]=308.24 hb.pfm (77499,77509) <=> (96659,96669) +

D[5373][6516]=329.58 c-FOS.pfm (77522,77529) <=> (96681,96688) +

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

77226 : tgactttctGTCAACAT-ctatccccactctgtgtcTGCCACCCCCt-cagcattaccaa

96388 : tgactttctGTCAACATtc-atccctactccacg-cTCCCCACCTCtacaacattaacaa

77284 : gaaaatacg-gat-gcctgCCCAGCTGTCCTgcttg-aTGTCACTGggtaaattatcttt

96446 : gaaaat--gagaacgcctgCCCAGATGTCCTg-ttggaTGTCACTGggtaaattatcttt

77341 : tattaagaatctgtaATTGTTCttcactgagctGTGACCTAAATTTAgtggtgctgagga

96503 : tattaagaatctgtaATTGTTCttcactgagccGTGACCTAAATTTAgcggtgctgaaga

77401 : gtgaGCCCCAGCCaacAGGATTTCCCtgagaacgtgatgaagTTCCTGTatcaattcaat

96563 : gtgaGCCCCAGCCaacAGGATTTCCCtgagaacacgatgaagTTCCTGTatcaattcgat

77461 : tcattaactagaaagagtgaacaagcagccaagtttttTTTTTTAATCCcctcattcttc

96623 : tcattaactagaaagagggaacaagcagcca-gcttt-TTTTTTCTGCCcc-cattcttc

77521 : aGTGAATCAtttgtttag--c

96680 : aGTGAATCAtttgtt-agtt-

### Alignment No 4 ###

D[2830][2974]=36.56 c-FOS.pfm (39909,39916) <=> (43120,43127) -

D[2834][2977]=77.31 Staf.pfm (39947,39966) <=> (43158,43177) -

D[2835][2978]=99.01 Dof2.pfm (39997,40002) <=> (43208,43213) -

D[2837][2979]=134.72 jsmad5.pfm (40010,40014) <=> (43221,43225) +

D[2841][2983]=166.10 SOX-9.pfm (40028,40036) <=> (43239,43247) -

D[2843][2987]=198.65 AGL3.pfm (40064,40073) <=> (43275,43284) +

D[2848][2988]=216.67 Sox-5.pfm (40123,40129) <=> (43334,43340) +

D[2856][3001]=231.37 Dof2.pfm (40174,40179) <=> (43385,43390) -

D[2857][3002]=258.54 HFH-1.pfm (40200,40210) <=> (43411,43421) -

D[2860][3005]=281.71 SOX17.pfm (40245,40253) <=> (43456,43464) +

D[2861][3008]=301.39 TCF11-MafG.pfm (40291,40296) <=> (43502,43507) +

D[2862][3009]=309.44 HMG-IY.pfm (40315,40330) <=> (43528,43543) +

Sequence 1: canFam2_dna

Sequence 2: hg18_dna

39900 : atatatgccTTAGTCATtagga-gatgtcctaaatattaccgtaaagcCCTTGGAATGAT

43111 : atatatgccTTAGTCATtaggatg-tgttctaaataatactgcaaagcCCTTGGAAGGAT

39959 : GGGTCTGAgccctcacttatacttaactgctggcatgtTGCTTTgtctc-cgTGTCTtgt

43170 : GGGTCTGAacactcacttatatttaactgctggcatgtTGCTTTgtccctg-TGTCTtgt

40018 : gctcctacttCCATTGATGtaaaggaagcacgaattagataacactCCATTATTAGggaa

43229 : gctactatttCCATTGATGtaaaggaagcaccaattaaataacactCCATTATTAGagaa

40078 : ccaagcacaagccagctgaggcaggggacccgccttctcacccagAAACAATgcgaagcc

43289 : ccaggcacaagtcagctgaggcaggagacccgccttcttttccagAAACAATgtaaagcc

40138 : tgcctggctgggggtccctgggcctcactca-tgct-cTGCTTTtgatattctccagcac

43349 : tgggtgggtgagggtctctgggcttc-cgccgtgcct-TGCTTTtgacattctccagcac

40196 : acacTATAAACATATctagggctgtcctgttcagtttgtttattcaaagTATATTGTCca

43407 : acccTATAAACATGTctaaggctgtcctgtttagtctgattattcaaacTATATTGTCca

40256 : gcatagagaaaagggaaatctagcaggactctggaGATGACaacagggagagagagagg-

43467 : gggtagagcaaagggaaacctagctgaaccctggaGATGACagcagggagagagagaggg

40315 : -CAGAAAAGGGCAAAAA---aaaaggcaaa

43527 : gCAAAGAAGGGCAAAACgggaaaaa-ca--

D. Human-mouse top candidates in region2:

### lambda=2 mu=0.5 nu=200 xi=200 Nucleotides per rotation=10.4

### D[mm9_dna][hg18_dna]

Note! First nucleotide at position 1 (one) and binding site at zero!

### Alignment No 1 ###

D[5659][6656]=44.43 E74A.pfm (81118,81124) <=> (99373,99379) -

D[5660][6657]=81.14 jsmad5.pfm (81130,81134) <=> (99385,99389) +

D[5661][6659]=127.60 Agamous.pfm (81135,81145) <=> (99390,99400) +

D[5664][6662]=145.37 MZF1-4.pfm (81177,81182) <=> (99431,99436) +

D[5666][6664]=193.77 Irf-2.pfm (81190,81207) <=> (99444,99461) -

D[5669][6667]=230.66 Yin-Yang.pfm (81214,81219) <=> (99468,99473) +

D[5676][6675]=224.66 SPI-B.pfm (81321,81327) <=> (99575,99581) +

D[5684][6682]=249.06 Bsap.pfm (81360,81379) <=> (99614,99633) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

81109 : aagaacttcCTTCCTGgcttgTGTCTCCTAAAAGGGGaaactactg-caaggtctctga-

99364 : aagagcttcCTTCCTGgcttgTGTCTCCAAAAGGGGAta-cga-tggcaaggtctgtaag

81167 : aaggaaggaaAGGGGAcctttttAATTAGCTTTCTCTTTTGaatgacGCCATCacagatc

99422 : a-ggaaggaaAGGGGAcctttttAATTAGCTTTCTCTTTTGaatgacGCCATCacagatc

81227 : tgattcatttccatgaattgaaaaaaacgaagcag--gg-taggtggtacacactc-cag

99481 : tgattcatttccatgaattgaaaaaaaaaaaaaagcagggtaggtggta-ag-cactctg

81283 : -aagaaatgaatgagagctgtcaggaaagagaacaggttAGAGGAAggct-acagctgga

99539 : taaggaatgaatgaaa-ctgtcagga--gagcacagattAGAGGAAagctga-agctgga

81341 : gaaaacaggcatgtggtac-TGGCTATTCATTACAACCCTgggccagttg

99595 : gaaaacagccatgtga-actTGGCCCTCCACCATGACCCAcgtttaggcc

### Alignment No 2 ###

D[2637][3627]=39.21 jsmad5.pfm (39008,39012) <=> (53806,53810) +

D[2638][3629]=76.01 Pbx.pfm (39018,39029) <=> (53816,53827) -

D[2641][3631]=92.45 cEBP.pfm (39067,39078) <=> (53866,53877) +

D[2643][3634]=111.99 E2F.pfm (39113,39120) <=> (53912,53919) +

D[2645][3636]=141.36 FREAC-4.pfm (39137,39144) <=> (53936,53943) +

D[2646][3637]=180.00 SOX17.pfm (39145,39153) <=> (53944,53952) -

D[2647][3639]=224.20 Athb-1.pfm (39165,39172) <=> (53964,53971) +

D[2649][3641]=233.21 Thing1-E47.pfm (39246,39255) <=> (54045,54054) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

38999 : gcatgaatcTGTCTaatggAATGAATGATCAaccctcggta-aacagattatgaaaacct

53797 : gcaagaattTGTCTaacagAATGAATGATCAaccttgagcagaagagattatgaaaaact

39058 : taacagcatTGTAGCAATGTGgttgctaataaaacacatttgactgcttccaccaTTTGG

53857 : taatagcatTGTAGCAATGTGgctgttaatgaaatacagttggctgctcccgctgTTTGG

39118 : CACcaatcgacccaacactGTCAACATCACAATATGagatttgttccCAATTATTtcatg

53917 : CACcaaccaacctgacactGTCAACATCACAATACGatatttattccCAATTATTttacg

39178 : gcaacaactgtaatacaatgtggtattaatcatatttattctaagtatcaatttgagaaa

53977 : gcaacaactgaaatacaatgtgttattaatcatatttattataagtatcaatttgagaaa

39238 : gttctgacGTGCCAGAAGatgaatcggc

54037 : tttctgacATGCCAGAAGataaataggt

### Alignment No 3 ###

D[5372][6491]=40.90 SP1.pfm (77505,77514) <=> (96422,96431) -

D[5380][6496]=52.66 Pax-2.pfm (77564,77571) <=> (96481,96488) +

D[5381][6497]=77.52 Sox-5.pfm (77601,77607) <=> (96518,96524) -

D[5384][6501]=135.61 RORalpha-2.pfm (77619,77632) <=> (96536,96549) -

D[5391][6507]=162.99 NF-kB.pfm (77662,77671) <=> (96579,96588) -

D[5392][6508]=198.64 HMG-1.pfm (77673,77681) <=> (96590,96598) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

77496 : acac-agggtCCCCAGCCCCcacaacattagcaagaaaataagaacgcctgcccagctgt

96413 : actccacgc-TCCCCACCTCtacaacattaacaagaaaatgagaacgcctgcccagatgt

77555 : cttgctggcTGTCACTGggtaaattatcttttattaaggatctgtaATTGTTCtccactg

96472 : cctgttggaTGTCACTGggtaaattatcttttattaagaatctgtaATTGTTCttcactg

77615 : agctGTGACCTAAATTTAgtagttctgaggagtgagacccagccaacAGGATTTCCCtGA

96532 : agccGTGACCTAAATTTAgcggtgctgaagagtgagccccagccaacAGGATTTCCCtGA

77675 : GAACACGatgaagttcc

96592 : GAACACGatgaagttcc

### Alignment No 4 ###

D[3299][4319]=39.60 SPI-1.pfm (47731,47736) <=> (63560,63565) -

D[3303][4321]=85.62 Pax-4.pfm (47739,47768) <=> (63568,63597) +

D[3304][4325]=103.65 MZF5-13.pfm (47809,47818) <=> (63638,63647) -

D[3306][4329]=134.54 Thing1-E47.pfm (47841,47850) <=> (63670,63679) +

D[3307][4332]=180.33 jtcf4.pfm (47854,47862) <=> (63683,63691) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

47722 : gtctggagtCTTCCCtgGAAACATATCCCTTGTGGCACCTCCCTTCT-ctgcaggatgct

63551 : gcctggagtCTTCCCtgGAAACATGTTCCCTTATCGCACCTCCCTTCtcttcaggatgct

47781 : aaa-atccgctggcctctatggactccatCCTCCCATACaattgactctccacacaatta

63611 : gagga-cctctggc-tccatggacttcatCCTCCCATACaattggttcccctcacaatta

47840 : tGTTCTGGCACaatCATCAAAGTgatctc-cctc

63669 : gTTTCTGGCACtgtCATCAAAGTgatccctc-tc

### Alignment No 5 ###

D[3329][4359]=36.55 E2F.pfm (48230,48237) <=> (64084,64091) -

D[3331][4360]=75.75 jsmad5.pfm (48238,48242) <=> (64092,64096) -

D[3334][4361]=99.20 tcf4.pfm (48271,48279) <=> (64125,64133) -

D[3338][4365]=120.82 jtcf4.pfm (48325,48333) <=> (64179,64187) +

D[3343][4368]=124.09 Dof2.pfm (48396,48401) <=> (64251,64256) +

D[3350][4372]=142.78 Broadc4.pfm (48426,48436) <=> (64280,64290) +

D[3354][4377]=156.58 HLF.pfm (48468,48479) <=> (64324,64335) -

D[3359][4380]=145.67 jsmad5.pfm (48536,48540) <=> (64395,64399) +

D[3366][4389]=144.54 E74A.pfm (48626,48632) <=> (64485,64491) +

D[3368][4390]=180.24 Dof2.pfm (48635,48640) <=> (64494,64499) -

Sequence 1: mm9_dna

Sequence 2: hg18_dna

48221 : gaggacaaaGAGCCAAAAGACAcagcatctctcttccaatgaatattcaaCTTCAAAGAa

64075 : gaggagaagGAGCCAAAAGACAcagcatctctcttcaaatgaatactcaaCTTCAAAGAa

48281 : gaaaagg-accggtggtacag-aggtgaagtcaaagcctccg-agtaCCTTTGAAGctag

64135 : gaaa-gtgaccactgtta-aggaggtgaagtcatagcctcggcaa-aCCTTTGAAGctgg

48338 : aggaaaagatgtaaattaagtgcgggtgagcaaaatatgaaagcaaaatcctggaagg-A

64192 : gggagaagatgtaaattaagcaccgacaagcagggtataaaaataaaatccttgaaggaA

48397 : AAGCAacccttcttccgcagtgggtgtgaAAGTAAAGGAAgtcag-ccaagtgacag-gg

64252 : AAGCAaaactgcttt-gcaatgggtgtgaAAGTAAAGGAAg-ctggccaag-gccagagg

48455 : a--taaacaggattgTGTGATGTAACCactctataaaca-ggccgaa-c-a-t-gagaag

64309 : aggtaaacagggttgCATGATGTAACCacactaggaa-aaggc--aaactagttgagaaa

48508 : caga-aggaaatacatggacgttgtaaacTGTCTattttatctgcagac-ttggaagaat

64366 : tatagagaaaatacatggaagttgtaaatTGTCTattttatctg-agaacttataagaat

48566 : caaagtgaaatcaatgtcaattttctcaatattcagctcattatacactctcatgaggga

64425 : caaagtggaaacaaggtcaattttcttaatattcaggccattataaacccttgtg-ggga

48626 : -CAGGAAGcaTGCTTTtatttgctgt

64484 : aCAGGAAActTGCTTTtatttgctgt

### Alignment No 6 ###

D[1836][2629]=41.38 E74A.pfm (26457,26463) <=> (37785,37791) -

D[1838][2631]=78.08 Dof2.pfm (26464,26469) <=> (37792,37797) +

D[1843][2636]=89.11 NRF-2.pfm (26524,26533) <=> (37853,37862) +

D[1846][2640]=127.69 NRF-2.pfm (26544,26553) <=> (37873,37882) +

D[1856][2646]=141.02 p65.pfm (26641,26650) <=> (37971,37980) +

Sequence 1: mm9_dna

Sequence 2: hg18_dna

26448 : ggatggaagCTTCCTGAAAGCAttgtggg-aaacatcctttgggtatgatacttacatga

37776 : agatggaagTTTCCTGAAAGCAttttggggaaacatcttttgggtatggttcttggatga

26507 : agagatgatttattagtCCTGGAAGAGtgtatgggagAGAGGAAGTGagaggttatgaga

37836 : agagttgatttattagtACTGGAAGGGtgtatgggagAGAGGAAGTGagaggttatgaga

26567 : gagtgaccctcctac-t-atggtgggtgggagagttcctgcaa-atgtatgttagctttg

37896 : gaatgaccctcc--cgtgatggtgagtgggagaattattgcagtatgtacgttagcattg

26624 : ctttttggtgaaaggt-tTGGGATTTCCtgggt-ctgaa

37954 : ctatgtggtgaa-gttctTGGGATTTCCtggggtccgt-

Sequences of entry clone inserts.

In order to construct the sequence of the final expression clones, simply insert the sequences of the enhancers (E03 through E31) and the sequence of the promoter/reporters (E01 or E02) into the appropriate region of the pDest-302 backbone vector.

6196-E01pMyc-Luc2-eGFP

AAAAAGATCCTCTCTGCTAATCTCCGCCCACCGGCCCTTTATAATGCGAGGGTCTGGACGGCTGAGGACCCCCGAGCTGTGCTGCTCGCGGCCGCCACCGCCGGGCCCCGGCCGTCCCTGGCTCCCCTCCTGCCTCGAGAAGGGCAGGGCTTCTCAGAGGCTTGGCGGGAAAAAGAACGGAGGGAGGGATCGCGCTGAGTATAAAAGCCGGTTTTCGGGGCTTTATCTAACTCGCTGTAGTAATTCCAGCGAGAGGCAGAGGGAGCGAGCGGGCGGCCGGCTAGGGTGGAAGAGCCGGGCGAGCAGAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGGCTTCGCCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGACCATGGAAGATGCCAAAAACATTAAGAAGGGCCCAGCGCCATTCTACCCACTCGAAGACGGGACCGCCGGCGAGCAGCTGCACAAAGCCATGAAGCGCTACGCCCTGGTGCCCGGCACCATCGCCTTTACCGACGCACATATCGAGGTGGACATTACCTACGCCGAGTACTTCGAGATGAGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGCTGAATACAAACCATCGGATCGTGGTGTGCAGCGAGAATAGCTTGCAGTTCTTCATGCCCGTGTTGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTACAACGAGCGCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGTATTCGTGAGCAAGAAAGGGCTGCAAAAGATCCTCAACGTGCAAAAGAAGCTACCGATCATACAAAAGATCATCATCATGGATAGCAAGACCGACTACCAGGGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATTTGCCACCCGGCTTCAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCATCGCCCTGATCATGAACAGTAGTGGCAGTACCGGATTGCCCAAGGGCGTAGCCCTACCGCACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCATCTTCGGCAACCAGATCATCCCCGACACCGCTATCCTCAGCGTGGTGCCATTTCACCACGGCTTCGGCATGTTCACCACGCTGGGCTACTTGATCTGCGGCTTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGCTATTCTTGCGCAGCTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTATTTAGCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTGCACGAGATCGCCAGCGGCGGGGCGCCGCTCAGCAAGGAGGTAGGTGAGGCCGTGGCCAAACGCTTCCACCTACCAGGCATCCGCCAGGGCTACGGCCTGACAGAAACAACCAGCGCCATTCTGATCACCCCCGAAGGGGACGACAAGCCTGGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTAAGGTGGTGGACTTGGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCGTCCGTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAACGCTCTCATCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTGGGACGAGGACGAGCACTTCTTCATCGTGGACCGGCTGAAGAGCCTGATCAAATACAAGGGCTACCAGGTAGCCCCAGCCGAACTGGAGAGCATCCTGCTGCAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCCTGCCCGACGACGATGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAACCATGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCCAAGAAGCTGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACTGACCGGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGCAAGATCGCCGTGCGGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGTAA

6196-E02pTK-Luc2-eGFP

CTAAATGAGTCTTCGGACCTCGCGGGGGCCGCTTAAGCGGTGGTTAGGGTTTGTCTGACGCGGGGGGAGGGGGAAGGAACGAAACACTCTCATTCGGAGGCGGCTCGGGGTTTGGTCTTGGTGGCCACGGGCACGCAGAAGAGCGCCGCGATCCTCTTAAGCACCCCCCCGCCCTCCGTGGAGGCGGGGGTTTGGTCGGCGGGTGGTAACTGGCGGGCCGCTGACTCGGGCGGGTCGCGCGCCCCAGAGTGTGACCTTTTCGGTCTGCTCGCAGACCCCCGGGCGGCGCCGCCGCGGCGGCGACGGGCTCGCTGGGTCCTAGGCTCCATGGGGACCGTATACGTGGACAGGCTCTGGAGCATCCGCACGACTGCGGTGATATTACCGGAGACCTTCTGCGGGACGAGCCGGGTCACGCGGCTGACGCGGAGCGTCCGTTGGGCGACAAACACCAGGACGGGGCACAGGTACACTATCTTGTCACCCGGAGGCGCGAGGGACTGCAGGAGCTTCAGGGAGTGGCGCAGCTGCTTCATCCCCGTGGCCCGTTGCTCGCGTTTGCTGGCGGTGTCCCCGGAAGAAATATATTTGCATGTCTTTAGTTCTATGATGACACAAACCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACACGCAGATGCAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAGGTGACGCGTGTGGCCTCGAACACCGAGCGACCCTGCAGCGACCCGCTTAAAAGCTTGATTCACCATGGAAGATGCCAAAAACATTAAGAAGGGCCCAGCGCCATTCTACCCACTCGAAGACGGGACCGCCGGCGAGCAGCTGCACAAAGCCATGAAGCGCTACGCCCTGGTGCCCGGCACCATCGCCTTTACCGACGCACATATCGAGGTGGACATTACCTACGCCGAGTACTTCGAGATGAGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGCTGAATACAAACCATCGGATCGTGGTGTGCAGCGAGAATAGCTTGCAGTTCTTCATGCCCGTGTTGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTACAACGAGCGCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGTATTCGTGAGCAAGAAAGGGCTGCAAAAGATCCTCAACGTGCAAAAGAAGCTACCGATCATACAAAAGATCATCATCATGGATAGCAAGACCGACTACCAGGGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATTTGCCACCCGGCTTCAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCATCGCCCTGATCATGAACAGTAGTGGCAGTACCGGATTGCCCAAGGGCGTAGCCCTACCGCACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCATCTTCGGCAACCAGATCATCCCCGACACCGCTATCCTCAGCGTGGTGCCATTTCACCACGGCTTCGGCATGTTCACCACGCTGGGCTACTTGATCTGCGGCTTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGCTATTCTTGCGCAGCTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTATTTAGCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTGCACGAGATCGCCAGCGGCGGGGCGCCGCTCAGCAAGGAGGTAGGTGAGGCCGTGGCCAAACGCTTCCACCTACCAGGCATCCGCCAGGGCTACGGCCTGACAGAAACAACCAGCGCCATTCTGATCACCCCCGAAGGGGACGACAAGCCTGGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTAAGGTGGTGGACTTGGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCGTCCGTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAACGCTCTCATCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTGGGACGAGGACGAGCACTTCTTCATCGTGGACCGGCTGAAGAGCCTGATCAAATACAAGGGCTACCAGGTAGCCCCAGCCGAACTGGAGAGCATCCTGCTGCAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCCTGCCCGACGACGATGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAACCATGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCCAAGAAGCTGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACTGACCGGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGCAAGATCGCCGTGCGGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGTAA

6196-E03enhancer D

TGAAGCCAATAACCTGGTGAATAATTCATTTTTCCAAGAAACAATTGCTAAGCCTCAGAATTGCTTTTTACCCAGACAGAGAGCAAATTATTTAACCAATAATAATACTTTCTGTATGTATAGAATCTTGTAATTTTTAAAATCATTCTCCTAACAATAAGGATAATGACCATAGCTAATATTTATCAAAAGCTTATCATGTGCCAAGTTTTGTGCTATGTCCTTCAACATAGATCATCTCATTTAATTTTTGCAACATTCTGAGACAGGTATTGCCATTTTTATCTCTCTTTTTCAGAGGAAGAAACTGAGGCTTATGAAGGTTAAGTAACTTTCCCAAGTCCACACAGCATTTAAATCCACATCACAGCCATCATGCTAGACTGCATCCCATTTCTTTATTGATCAGTAGACAGATAGAAGCAGACATATAGACTCATTTGATAATTATGAAAACCCTGGGTGACAAAAAGTATCACATATTAGGGCTACCAAATCAGATAAGAAAATTTAGGCTACAGATTTAAAACAACCTGTTCAAGTAACACATTAGCAAGGCAAAATCAGTTTTCTGATCTGAGTCCTGTGGTCCTCCCATAGCCCTGGGGCAACTCTAACAGAATCAAAAAGATTTATTTAATTTTTGCCTTGCAGTGTGCATGGGAGTGTGTCAGGGGAAAGGGACCGGATACGGCAGTGTACCGAGCCCTACGATGACATTTTCAGCCAAGAAGAAGAAGAATGCTTCATCAAATCTCCCATCAGCCCAAGACTAGATTGTACCAAGATTTTGCAATATGAACATAATGTTCTCTACTGTCCAGGATCTAATCCTTTTCCATCGGGGAACACATTTGCAAACAATGACTCCTAACTGATGAGGAACAGGATCCAACTGTTACTCCACAAACTAGGACCTTTACACCACATTGGAGGCAATAGCCCTGAGGTTAAAGGGCCGTTCCGTGTCTAACCACTGAAGCTCTTGCTTGAAGGAAGGTTTCCCAAAATGAACCCCCAAAGAAACTGAAAATGGCATGAACGTACATCCTGTTACTCAACACTCCCCGAGTTTCATAACGTGAAGTCAGGAAGAAAAGGTGCCAACATTCCTGAATGTTATTATCATTAAGGAGCAAACTCTTAATGTTCCCACACTTGAACGTTAAAGAGATGGGATCATTCAGTCTCCTCTTTACTTATGGAATTGCTGCAAAACCATATGGGCTGAATTAGGCATCCAAGGCTAATGTTTCCTACCATTCCCTCTGGGGGGCCTTTGGGGCTCATCCCAGGCACTTGGCTTTAAAGACAAGGATATCCTATTTGGAAACACAGTCATGAGCCCTGATATTAATAAGCACTTCCTGACTTCAAAGCATCTTTATAACCATTGCCCTAATTAAATCTCGTAACCTCCCAGAGAGAATGCAGGCTTCATCATTTCCACTTTACAAATGGAGAAACTGAGGCAGAGCCCTATGGAATATGAAACCGTATCCCACCTGATTGAGGAACCACTGGAGATTTTAATCAGTTTCAGATTAAATTACAATGTTAAAGTTTTAATTTTTTATTTTACCATTTGGTGAGATTAAAACAAGGCATTTAATTGTGTATTCTGATGGTTAAAACCACCAGACCTGGTTGTGTGGCCTTGGCATAGGGATCAAACTTCTCTAAACTTTAATTTCCTCATTGGGAAAAATGAAAATAATAACAGAGTCTACCATAATGTTTTTCAGGTTAAATAATGTATGTTAAGTTTCTGGCATGCAAATAAGCACTCAATAAATTGTAGCAGTTTAAGTCAGCTTATTTTCCTTCAGCTACCCATTCATTCCTTCATTCACTCGTTTCCTAGTATTTATTACAGCCCATTGAGTGCTGAAGGCTGTGCAGTCCACAAAGGATACAGCAGCGAACAAAGCAGAAAAGGGACTATTTCCTACATATGGAATATATCTTATTTATGATTATAAATTAATTCACACAAAATGTGGGTTATTCCCTATTGATTTTGAACTGATCTCACCACTTACTTATAAGGTCTCTTTGATTGTTCTTTCAGCTGAACCATCACATTCCTTATACAGAGTGGGACAGCATTGGCTGAGTTCTACTAGCAAATGCCGGAGATGTGGTTCCTGTTGGCCTTTTA

6196-E04enhancer G

GAATTTGACCAAAGAAGCCAATAGAACTATTCCAAAAACCTATGGTTCCCCCTAAAGCATTAGAAAGACTCAGAACGGGTTAAGTGCTCCCTGGCTCATTCCCAACAGACACTACATTCACCTGTGCTTGCTCTGAAATAAATCAGTGTCCCTTTCTGCTGCTGCTGTTGTCTGGAAATAATGCAAATGCAATGGGCCTTTACTGACATTGTGCTTCCCTGGAAGGATACACATAATAAATTATCCCTTAATACTGTTAAAGAGACATTTTCCTCTTACTCAGGAGCTTTTGGGGTTGGACTGGGCTACTCACCCAGCAAGGAGGAGGACATGTGTCTTGTCACTGGCCCGGTTATTCATGTGGCCTCTCATTGCTCCTTGGCTCACTGCATTGCAAGATTCAAGGATGCACTTCCAGGCCTCCACATCAAGTCATAGGACTTGCCGGTAACCTAGATTGGTTTTCTCATTTGTAATTTGAATTTATTTTATGTTATGCATTTGTATGTTTATTTATTCGGATGCTCAGAAGCTGAAGATAACTAGTGCTCCTGGTCCATGCCATTCATCAATTGGAAGAATGCCAAGCTGTTTCCGCTGAGGACAGAAGGCATTGGTCTCCCCTGCAGGAAGCCACTGCTGCTCCTTAATTGTTTGCTAGAGGAAGAATCAAGGGTAAAATTTAAAGTAAATGGCTGGCCGAGTTGCACTAATTCATCAAAGCATGTTTCAAGTCAGTAGTCAGAGCATGCATCAGCCCCCGGCGCCACCAGCTTCTACGAGAGTGGAAAAGCCAGCAGACCTCCGAGCAGATGAAATCATTAGGAGGCATTCAGCAGGGCTTGAAAAGCAAAGAGAGAGGAGGCGGGGATTTCTCTGCATGCTCCCTTTGCCACATGGGAAACACCAGCTGTCTGTGACCTAGTTATCCAAGAAAGGAAACACGGAAGAGAACCCACAAAACTGTTTGCTACATGAGAACCCC

6196-E05enhancer E T allele

GGTATGCTAAAGTTTGCAAAACTGGTATAGACACCAAGAGGGAGGTATCAACAGAGACTCCCCAAGAGCTAAGAGGAAACCACCTTGGACTGGAAGTCAAGAGCCAAAATTCTAGACTCTACTCTGTAATTAACTACCTATTTGAATTTGGAAAAATCACCATCAACTTTCCCAGCCTCGTTCTCTGCATCTGGGAAATGAAGGCGTCGTCCAAATGATTACAAGCTTCTTTTCCTGCTCTTATTGCATGATTCCACTTCCACAGCCCTCCAGCATTTTTTAGCAGCTGCATCGCTCCATAGAGCCTGCAGAGGGCACTAGACTGGGAATTAGAAAACCTGATTTCCCTTCCAGCTCCACCTCTGACCAATTGCCTGACCCTGGTCAAATTGCTTAACCTCTTCCTATCTCAGCTCCCTATCCATAAAACAGAGGGACGAATAAACTCTCCTCCTACCACTAAGAGGTGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAGTCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTATTTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAATCAAAGGGCAGGAACCCAGCAAGTTTCTCAGGATTGGGGCCTTAGACTGGGCTGTGTATACAGACAGTGCCAGCCAACCCCACAGTTCAGTTTCCTTTAACCTGGTGCTCCAGGCAATAACTGTGCAACTCTGCAATTTAACAATGTGTTCTTTGTCCCACAACTGTTCTCGTTTCTCAACTGCCCAGGTAATATGTTTGGGCCTGTAGGAAGAGTCAAATAGTTAATAAGGGAAGGGTTTGGCATGCCCTACGTAAGTTCTACCAGCAAGTCCCAACAAGAAGGCATTCTGTGTCTCCTGATTCCTGACCTACCCCCAAAATGTACAAATGTACAAGGAATGAGCCCACTTTCCCAGCAGGCTGTAATACCAGTTTGGCCTATATCAATGCATTGGTGAGCTGTGTTTTGTTTATGGTTTTATGCCATCTATTTTCCCATGGATATTATGTTTTCTAAAGAGCCCTTAAGTTTACGTCAGCTTTTAAAGCTACCAGCAGCACCATTTCAGTTCATATTAAGCCCTTAATATGGTATGAATAGGAGAGCTATTAGACTAAAGAGCCATAATCATCCCTGAGGAAAACATCCATCACCAACATTTATGTGGTCCCTGAACTTCTAAAAGGTGTCATCTCTCTGGGGTGTATCTGGTGAGAGCTTTCTCTGGGAGATGCCAAAAAGCCAATGCATTAGATGAAGCTTAGAAGGGCATTTTCTAACCATTACAAATTGCCTAGTCTAGCATCTCAATTTCATCTACGTGAAGAGCCTTAATTAAATTTGTTGGGGTTTGATCCTTTATCCCCAGATGTGGCGCTGACAGAGATTGCTTACATAAATAATGTGTGCTCCAAGTGCTTGCCAGGCTCCTGGCTCAGCTGGGACAGCTGTAGCTTTTTGAATGTCATTCCCAAGATATCCTGCAGGTGTTCAGCTTCCCCTGTTCTACTCTGGGAAGAGAGCCGTGGGCAACATCAGCCCAGAAGACACTAGTGCATCTTGACAGAGCATTCCCACTGGACAGGTGCACTACCTGCAGACACCTCCAACAGTGAAAGCACCCCCCGGCTGCAAGTATTGTCTCGCCAAACGGAAGGCATTTCTTGTATGAGTCTTGAATCTGATATTCTTTAATAAGACTTTCTCTAAAACTGTATACACACACGCACCTGAAAACGTTATCAGGCTTTTACTTATTTGACAGTAATATTCCAGGAAATGGTCCAAGTAGCAAAATCTTCTCTCAGTGGCTTTCTCGTTCCTCATGTCTGACTTGCTTTCCTGCTGTCTACTGGAGCCAACAAGAGAGGCATTCTCACTCTGGAAGTTGTTAGTTCATTACAGCCTAGGTTGATCTCATCTGGCTCTGGTTGGGGGTTTTATTCTGAAGATTGGCTGGTAAAAGCCTCCACAAAGTCTTAGCTAGAACATTCATAGCCCTGTGTTGACTTCCGCCTCAACTCTGGCTTGAACTTAGCTGACGGTCCTATCAGTTTGCTCAAAGGAGCTTT

6196-E06enhancer F

CTCCAGTGTAAGAATGGTGACACTAAGATATCCAAGAGAGGGATCTTAGACTCAGGACAGGTTATATGCAAAAGACAGTTTACAGCCTTAAAGGTTATAGAATCCAGCCTGCTACTGAGAAAGCAAAGGCTAGAGACATCCCAAGATGACACAGTGGACGACCAAATGCTCACCACTGTTTTCTGGGGCTCTAACTCCAGGCTGTTGATTCTCAGGAAGGGTTGTTTTTCATACTGCCCTTTCAAATTACAGCACCTTTGCTGCCTCCATCTAAAACTGGCCCTTCCAACATCTCATCAACTTTTACATTCTGATGTCCCCATTTCAGAGAGATTTCTTTGAAACAGAGCCAGCCCTTCCAGTAAGCATATATAACAGAATGGTGATTTCAGAGAGTGGAGCCTGGTGGAAACAAAACCCAGACATGTAAAATTTGACACCTTAACCGACATAAGTGCCCACATTCCCTGCGTTTTCCCACTCTCTGCCTCACCCCACCTTCAACATGCCCATAATTTTATTCTGAGGAAAACCACAACCAGCACTTACCACCAGAGTCCAAATTCCCTGGCAGCAGCCTTGCTTTTAAAAGGGAGACATAAAAAAAATTGTCAAGACCTATGGGGCTTCCTAACACATTAAAAAGGCCCTCAAAAACTGTGTTACACCAAGCATAATATTATCTTTTTCTTTAAGAGATTTCTTAATTGGTTCCATGCAAAAGGTTTGTAGGTAATGGGTTTTAAATAAATAATAATGTTCCACCACCCTGACTGGCCAAGAAAGTAAATGCCTTTTTCCCTGACTTGGGTAAGCACTGGGATGGAAAAAAGGCCTGTGTGAAAATTTTGGCCTCACCGTAAAAAGGCAAGACCTCAATCTGGGGACTCAGATGTAATCAACCATGGTTTAGTTTCAGTAATTTGGTTGCAACAATTATTTGGCAAGCATAGTGCTAGGTATTGGTGGGACGGAGATGACAGACTGTATAACTGAATGCTCAGAATAATTAAGTTAAATGTATT

6196-E07enhancer B

ATAATATTGTTAATAAAATAACCCAGCCAGAGGTAGAACCCAAGATTCCGACTCTAGTTGACCTGATTCTAAGCCAACTCTTCTACAGCAACACATCGGCCCATTGATTTAGACAGCATGGAACTCATAGCCCAGGGAATTCACACTCTTAAAGAACTTAAACAGCACAGCTTAATATGGTCCCTTCCAAACATACGACTCAGTAATTCCAGTAGGAGGGCTAAACAACCTATTCTGAGAGGTCTCCAGGAAAGGAAGCCTGGAGTCTTCCCTGGAAACATGTTCCCTTATCGCACCTCCCTTCTCTTCAGGATGCTGAGGACCTCTGGCTCCATGGACTTCATCCTCCCATACAATTGGTTCCCCTCACAATTAGTTTCTGGCACTGTCATCAAAGTGATCCCTCTCCAAGGACATGGAACAGGATGAAAAACAAATTGTAATTGTAAACTCGAAGTACGATCAGGGCAGGCACAGCAAAGGAGGAAGGTTACGTTGTGGGAATCAGGGGTCAACAATAAAAGCCAGCCTGCTGCCTGTCCCCACCAGGCAATTTTCTGGGCTGTTCATGTCCCCAGTTATGCCCCCAAACATTCCCATGACAGCAATGCCTAAGGAAAATAACCTCTGAAGATTGAGCCTAAAACTAGAATCATTTTTCTCTCTCATTCTAATTCATAGCAGGGAAGTGGACTGGCTCCTTAAATCCAGAAAGTATCAGACTTATCTGATCAAATCATTAGGGCAACTGTGTTTTCCCTCATCTCCAGACAGCATGGATGAGGAGAAGGAGCCAAAAGACACAGCATCTCTCTTCAAATGAATACTCAACTTCAAAGAAGAAAGTGACCACTGTTAAGGAGGTGAAGTCATAGCCTCGGCAAACCTTTGAAGCTGGGGGAGAAGATGTAAATTAAGCACCGACAAGCAGGGTATAAAAATAAAATCCTTGAAGGAAAAGCAAAACTGCTTTGCAATGGGTGTGAAAGTAAAGGAAGCTGGCCAAGGCCAGAGGAGGTAAACAGGGTTGCATGATGTAACCACACTAGGAAAAGGCAAACTAGTTGAGAAATATAGAGAAAATACATGGAAGTTGTAAATTGTCTATTTTATCTGAGAACTTATAAGAATCAAAGTGGAAACAAGGTCAATTTTCTTAATATTCAGGCCATTATAAACCCTTGTGGGGAACAGGAAACTTGCTTTTATTTGCTGTTTCTGCAATAGTGTTGTTTGGGTACACGACAGGACCATGGAACTCTATAATCTTGTATAGGGATACATGCCAAGTGCCATTCCTGATTCCTTTGTAGTTTTTTTTTTTTCTTATGTCTCCTATTAGTTGAATAATTCATTTTTTGTTTATTTATTCATTCATTCAATAAACTGCTTTTGTGCTTACAGTATACCAGACACTCTACTAGATGCTAAATAAGCAAAGATGAACATGGCCTTGTCCTTACCCTTAAAGAACCATAACTCTATATAAAAACACATTTAATTTGTAAATTAGAGGACAGGATAGTTAGTTTGTGATAGAGA

6196-E08enhancer A

GTCCTGGTGTCTTTCTCATGCTCTTTGATTAGTCCTTAATCCACAAGCGCAACATTGCAATACTTGCCTAGCATATTTCACAGGCAGGGGACCTAATGTCCCTGTGAGAACCCATCTTGCTGAGATTGTCGCTGGCAGATTTACTTCCAGTGTGATTGTTGCAAGAATTTGTCTAACAGAATGAATGATCAACCTTGAGCAGAAGAGATTATGAAAAACTTAATAGCATTGTAGCAATGTGGCTGTTAATGAAATACAGTTGGCTGCTCCCGCTGTTTGGCACCAACCAACCTGACACTGTCAACATCACAATACGATATTTATTCCCAATTATTTTACGGCAACAACTGAAATACAATGTGTTATTAATCATATTTATTATAAGTATCAATTTGAGAAATTTCTGACATGCCAGAAGATAAATAGGTTTATTATGAAAAGCAGTTCTGCTTGGTGCATGCTGGCTGCTGCTGTGTAATAAATAGCCTCTGTGGGGAAAGTTTTTTAAAAGAAATAAAGCAAAAAAATAGCACTGAAAACAGAAAGGAAGCATCAAAACTCTTCAAATACCTGCTGTGTCCATTGGTCAAGCACATTCAGG

6196-E09enhancer C

AGGAGGTGGGACAGATAAATCACAGTTTGTGAAGAGCCATAAAATAATTCAGCATGGCTAAAGAGTAGAGAGAGCAGTTAGTAGAGAGCAGAAGGTAAGGTAGAAAAAGATTAATCCAGAGAAGGCATGAGCAACCAGGTTTCAAATGCAGGACTTATGAATTCTGCCTTCCCACTACTAAGCTTCTTAGCATTTTTTTGTCCTGGCCCAATTTAGAAAACCATTTTGTGGGGGTCCCATTAGGTTCAGCTTTCAGTTGCTGGAAGAGCATGGACTGACATATGCCACAAAGCAGGTCAGAAAATGACTTTCTGTCAACATTCATCCCTACTCCACGCTCCCCACCTCTACAACATTAACAAGAAAATGAGAACGCCTGCCCAGATGTCCTGTTGGATGTCACTGGGTAAATTATCTTTTATTAAGAATCTGTAATTGTTCTTCACTGAGCCGTGACCTAAATTTAGCGGTGCTGAAGAGTGAGCCCCAGCCAACAGGATTTCCCTGAGAACACGATGAAGTTCCTGTATCAATTCGATTCATTAACTAGAAAGAGGGAACAAGCAGCCAGCTTTTTTTTTCTGCCCCCATTCTTCAGTGAATCATTTGTTAGTTTGACCAGAATTTTTGTGAGATCCAGTCTACAATTCTGTAAGAAAAAAAGACAGCAAACCTCATTCAAATACTTCTTGCGACATTAATTCCGAGGCAGAAACCAAATTACATTAAGCAGATCTGATTGTCTTGCTATTAAAGACCTTGGGTTTACTCACTGAGTATTTTCAGCCTGAGGAGAAGAAATGCTCTCAATTTGGGTAGGGTGTTTGGTAACTCGGGATAAAATGAATAAGAGCCAACTTGGCTCACAAGTGGATTTAAAAACAGGAAGAAAGAGAGAGAAAAAAATAAGAAAAAGGCAAATACAATACAAACCTAGTACAAGCTTCCAGTGAAAATTTGCGTGTTTGTAATAAAGCTTATAAGAAGGAAGAGAACCAATATAAATTCTTGCTATGTCTAAGAACGCCTTTAATTATGACAGCCCAGTTGAGGTCCAGAGAGGGACACACAGTTT

6196-E10CMV enhancer

AGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGA

6196-E11control enhancer

TAATAAGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAG

6196-E21enhancer E Tcf4 triple mutant

GGTATGCTAAAGTTTGCAAAACTGGTATAGACACCAAGAGGGAGGTATCAACAGAGACTCCCCAAGAGCTAAGAGGAAACCACCTTGGACTGGAAGTCAAGAGCCAAAATTCTAGACTCTACTCTGTAATTAACTACCTATTTGAATTTGGAAAAATCACCATCAACTTTCCCAGCCTCGTTCTCTGCATCTGGGAAATGAAGGCGTCGTCCAAATGATTACAAGCTTCTTTTCCTGCTCTTATTGCATGATTCCACTTCCACAGCCCTCCAGCATTTTTTAGCAGCTGCATCGCTCCATAGAGCCTGCAGAGGGCACTAGACTGGGAATTAGAAAACCTGATTTCCCTTCCAGCTCCACCTCTGACCAATTGCCTGACCCTGGTCAAATTGCTTAACCTCTTCCTATCTCAGCTCCCTATCCATAAAACAGAGGGACGAATAAACTCTCCTCCTACCACTAAGAGGTGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGGCGAAAGTCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTATTTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAGCCCAAGGGCAGGAACCCAGCAAGTTTCTCAGGATTGGGGCCTTAGACTGGGCTGTGTATACAGACAGTGCCAGCCAACCCCACAGTTCAGTTTCCTTTAACCTGGTGCTCCAGGCAATAACTGTGCAACTCTGCAATTTAACAATGTGTTCTTTGTCCCACAACTGTTCTCGTTTCTCAACTGCCCAGGTAATATGTTTGGGCCTGTAGGAAGAGTCAAATAGTTAATAAGGGAAGGGTTTGGCATGCCCTACGTAAGTTCTACCAGCAAGTCCCAACAAGAAGGCATTCTGTGTCTCCTGATTCCTGACCTACCCCCAAAATGTACAAATGTACAAGGAATGAGCCCACTTTCCCAGCAGGCTGTAATACCAGTTTGGCCTATATCAATGCATTGGTGAGCTGTGTTTTGTTTATGGTTTTATGCCATCTATTTTCCCATGGATATTATGTTTTCTAAAGAGCCCTTAAGTTTACGTCAGCTTTTAAAGCTACCAGCAGCACCATTTCAGTTCATATTAAGCCCTTAATATGGTATGAATAGGAGAGCTATTAGACTAAAGAGCCATAATCATCCCTGAGGAAAACATCCATCACCAACATTTATGTGGTCCCTGAACTTCTAAAAGGTGTCATCTCTCTGGGGTGTATCTGGTGAGAGCTTTCTCTGGGAGATGCCAAAAAGCCAATGCATTAGATGAAGCTTAGAAGGGCATTTTCTAACCATTACAAATTGCCTAGTCTAGCATCTCAATTTCATCTACGTGAAGAGCCTTAATTAAATTTGTTGGGGCTTGATCCTTTATCCCCAGATGTGGCGCTGACAGAGATTGCTTACATAAATAATGTGTGCTCCAAGTGCTTGCCAGGCTCCTGGCTCAGCTGGGACAGCTGTAGCTTTTTGAATGTCATTCCCAAGATATCCTGCAGGTGTTCAGCTTCCCCTGTTCTACTCTGGGAAGAGAGCCGTGGGCAACATCAGCCCAGAAGACACTAGTGCATCTTGACAGAGCATTCCCACTGGACAGGTGCACTACCTGCAGACACCTCCAACAGTGAAAGCACCCCCCGGCTGCAAGTATTGTCTCGCCAAACGGAAGGCATTTCTTGTATGAGTCTTGAATCTGATATTCTTTAATAAGACTTTCTCTAAAACTGTATACACACACGCACCTGAAAACGTTATCAGGCTTTTACTTATTTGACAGTAATATTCCAGGAAATGGTCCAAGTAGCAAAATCTTCTCTCAGTGGCTTTCTCGTTCCTCATGTCTGACTTGCTTTCCTGCTGTCTACTGGAGCCAACAAGAGAGGCATTCTCACTCTGGAAGTTGTTAGTTCATTACAGCCTAGGTTGATCTCATCTGGCTCTGGTTGGGGGTTTTATTCTGAAGATTGGCTGGTAAAAGCCTCCACAAAGTCTTAGCTAGAACATTCATAGCCCTGTGTTGACTTCCGCCTCAACTCTGGCTTGAACTTAGCTGACGGTCCTATCAGTTTGCTCAAAGGAGCTTT

6196-E24enhancer E Tcf4 site1 mutant

GGTATGCTAAAGTTTGCAAAACTGGTATAGACACCAAGAGGGAGGTATCAACAGAGACTCCCCAAGAGCTAAGAGGAAACCACCTTGGACTGGAAGTCAAGAGCCAAAATTCTAGACTCTACTCTGTAATTAACTACCTATTTGAATTTGGAAAAATCACCATCAACTTTCCCAGCCTCGTTCTCTGCATCTGGGAAATGAAGGCGTCGTCCAAATGATTACAAGCTTCTTTTCCTGCTCTTATTGCATGATTCCACTTCCACAGCCCTCCAGCATTTTTTAGCAGCTGCATCGCTCCATAGAGCCTGCAGAGGGCACTAGACTGGGAATTAGAAAACCTGATTTCCCTTCCAGCTCCACCTCTGACCAATTGCCTGACCCTGGTCAAATTGCTTAACCTCTTCCTATCTCAGCTCCCTATCCATAAAACAGAGGGACGAATAAACTCTCCTCCTACCACTAAGAGGTGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGGCGAAAGTCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTATTTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAATCAAAGGGCAGGAACCCAGCAAGTTTCTCAGGATTGGGGCCTTAGACTGGGCTGTGTATACAGACAGTGCCAGCCAACCCCACAGTTCAGTTTCCTTTAACCTGGTGCTCCAGGCAATAACTGTGCAACTCTGCAATTTAACAATGTGTTCTTTGTCCCACAACTGTTCTCGTTTCTCAACTGCCCAGGTAATATGTTTGGGCCTGTAGGAAGAGTCAAATAGTTAATAAGGGAAGGGTTTGGCATGCCCTACGTAAGTTCTACCAGCAAGTCCCAACAAGAAGGCATTCTGTGTCTCCTGATTCCTGACCTACCCCCAAAATGTACAAATGTACAAGGAATGAGCCCACTTTCCCAGCAGGCTGTAATACCAGTTTGGCCTATATCAATGCATTGGTGAGCTGTGTTTTGTTTATGGTTTTATGCCATCTATTTTCCCATGGATATTATGTTTTCTAAAGAGCCCTTAAGTTTACGTCAGCTTTTAAAGCTACCAGCAGCACCATTTCAGTTCATATTAAGCCCTTAATATGGTATGAATAGGAGAGCTATTAGACTAAAGAGCCATAATCATCCCTGAGGAAAACATCCATCACCAACATTTATGTGGTCCCTGAACTTCTAAAAGGTGTCATCTCTCTGGGGTGTATCTGGTGAGAGCTTTCTCTGGGAGATGCCAAAAAGCCAATGCATTAGATGAAGCTTAGAAGGGCATTTTCTAACCATTACAAATTGCCTAGTCTAGCATCTCAATTTCATCTACGTGAAGAGCCTTAATTAAATTTGTTGGGGTTTGATCCTTTATCCCCAGATGTGGCGCTGACAGAGATTGCTTACATAAATAATGTGTGCTCCAAGTGCTTGCCAGGCTCCTGGCTCAGCTGGGACAGCTGTAGCTTTTTGAATGTCATTCCCAAGATATCCTGCAGGTGTTCAGCTTCCCCTGTTCTACTCTGGGAAGAGAGCCGTGGGCAACATCAGCCCAGAAGACACTAGTGCATCTTGACAGAGCATTCCCACTGGACAGGTGCACTACCTGCAGACACCTCCAACAGTGAAAGCACCCCCCGGCTGCAAGTATTGTCTCGCCAAACGGAAGGCATTTCTTGTATGAGTCTTGAATCTGATATTCTTTAATAAGACTTTCTCTAAAACTGTATACACACACGCACCTGAAAACGTTATCAGGCTTTTACTTATTTGACAGTAATATTCCAGGAAATGGTCCAAGTAGCAAAATCTTCTCTCAGTGGCTTTCTCGTTCCTCATGTCTGACTTGCTTTCCTGCTGTCTACTGGAGCCAACAAGAGAGGCATTCTCACTCTGGAAGTTGTTAGTTCATTACAGCCTAGGTTGATCTCATCTGGCTCTGGTTGGGGGTTTTATTCTGAAGATTGGCTGGTAAAAGCCTCCACAAAGTCTTAGCTAGAACATTCATAGCCCTGTGTTGACTTCCGCCTCAACTCTGGCTTGAACTTAGCTGACGGTCCTATCAGTTTGCTCAAAGGAGCTTT

6196-E25enhancer E Tcf4 site3 mutant

GGTATGCTAAAGTTTGCAAAACTGGTATAGACACCAAGAGGGAGGTATCAACAGAGACTCCCCAAGAGCTAAGAGGAAACCACCTTGGACTGGAAGTCAAGAGCCAAAATTCTAGACTCTACTCTGTAATTAACTACCTATTTGAATTTGGAAAAATCACCATCAACTTTCCCAGCCTCGTTCTCTGCATCTGGGAAATGAAGGCGTCGTCCAAATGATTACAAGCTTCTTTTCCTGCTCTTATTGCATGATTCCACTTCCACAGCCCTCCAGCATTTTTTAGCAGCTGCATCGCTCCATAGAGCCTGCAGAGGGCACTAGACTGGGAATTAGAAAACCTGATTTCCCTTCCAGCTCCACCTCTGACCAATTGCCTGACCCTGGTCAAATTGCTTAACCTCTTCCTATCTCAGCTCCCTATCCATAAAACAGAGGGACGAATAAACTCTCCTCCTACCACTAAGAGGTGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAGTCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTATTTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAATCAAAGGGCAGGAACCCAGCAAGTTTCTCAGGATTGGGGCCTTAGACTGGGCTGTGTATACAGACAGTGCCAGCCAACCCCACAGTTCAGTTTCCTTTAACCTGGTGCTCCAGGCAATAACTGTGCAACTCTGCAATTTAACAATGTGTTCTTTGTCCCACAACTGTTCTCGTTTCTCAACTGCCCAGGTAATATGTTTGGGCCTGTAGGAAGAGTCAAATAGTTAATAAGGGAAGGGTTTGGCATGCCCTACGTAAGTTCTACCAGCAAGTCCCAACAAGAAGGCATTCTGTGTCTCCTGATTCCTGACCTACCCCCAAAATGTACAAATGTACAAGGAATGAGCCCACTTTCCCAGCAGGCTGTAATACCAGTTTGGCCTATATCAATGCATTGGTGAGCTGTGTTTTGTTTATGGTTTTATGCCATCTATTTTCCCATGGATATTATGTTTTCTAAAGAGCCCTTAAGTTTACGTCAGCTTTTAAAGCTACCAGCAGCACCATTTCAGTTCATATTAAGCCCTTAATATGGTATGAATAGGAGAGCTATTAGACTAAAGAGCCATAATCATCCCTGAGGAAAACATCCATCACCAACATTTATGTGGTCCCTGAACTTCTAAAAGGTGTCATCTCTCTGGGGTGTATCTGGTGAGAGCTTTCTCTGGGAGATGCCAAAAAGCCAATGCATTAGATGAAGCTTAGAAGGGCATTTTCTAACCATTACAAATTGCCTAGTCTAGCATCTCAATTTCATCTACGTGAAGAGCCTTAATTAAATTTGTTGGGGCTTGATCCTTTATCCCCAGATGTGGCGCTGACAGAGATTGCTTACATAAATAATGTGTGCTCCAAGTGCTTGCCAGGCTCCTGGCTCAGCTGGGACAGCTGTAGCTTTTTGAATGTCATTCCCAAGATATCCTGCAGGTGTTCAGCTTCCCCTGTTCTACTCTGGGAAGAGAGCCGTGGGCAACATCAGCCCAGAAGACACTAGTGCATCTTGACAGAGCATTCCCACTGGACAGGTGCACTACCTGCAGACACCTCCAACAGTGAAAGCACCCCCCGGCTGCAAGTATTGTCTCGCCAAACGGAAGGCATTTCTTGTATGAGTCTTGAATCTGATATTCTTTAATAAGACTTTCTCTAAAACTGTATACACACACGCACCTGAAAACGTTATCAGGCTTTTACTTATTTGACAGTAATATTCCAGGAAATGGTCCAAGTAGCAAAATCTTCTCTCAGTGGCTTTCTCGTTCCTCATGTCTGACTTGCTTTCCTGCTGTCTACTGGAGCCAACAAGAGAGGCATTCTCACTCTGGAAGTTGTTAGTTCATTACAGCCTAGGTTGATCTCATCTGGCTCTGGTTGGGGGTTTTATTCTGAAGATTGGCTGGTAAAAGCCTCCACAAAGTCTTAGCTAGAACATTCATAGCCCTGTGTTGACTTCCGCCTCAACTCTGGCTTGAACTTAGCTGACGGTCCTATCAGTTTGCTCAAAGGAGCTTT

6196-E26enhancer E Tcf4 site1/3 mutant

GGTATGCTAAAGTTTGCAAAACTGGTATAGACACCAAGAGGGAGGTATCAACAGAGACTCCCCAAGAGCTAAGAGGAAACCACCTTGGACTGGAAGTCAAGAGCCAAAATTCTAGACTCTACTCTGTAATTAACTACCTATTTGAATTTGGAAAAATCACCATCAACTTTCCCAGCCTCGTTCTCTGCATCTGGGAAATGAAGGCGTCGTCCAAATGATTACAAGCTTCTTTTCCTGCTCTTATTGCATGATTCCACTTCCACAGCCCTCCAGCATTTTTTAGCAGCTGCATCGCTCCATAGAGCCTGCAGAGGGCACTAGACTGGGAATTAGAAAACCTGATTTCCCTTCCAGCTCCACCTCTGACCAATTGCCTGACCCTGGTCAAATTGCTTAACCTCTTCCTATCTCAGCTCCCTATCCATAAAACAGAGGGACGAATAAACTCTCCTCCTACCACTAAGAGGTGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGGCGAAAGTCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTATTTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAATCAAAGGGCAGGAACCCAGCAAGTTTCTCAGGATTGGGGCCTTAGACTGGGCTGTGTATACAGACAGTGCCAGCCAACCCCACAGTTCAGTTTCCTTTAACCTGGTGCTCCAGGCAATAACTGTGCAACTCTGCAATTTAACAATGTGTTCTTTGTCCCACAACTGTTCTCGTTTCTCAACTGCCCAGGTAATATGTTTGGGCCTGTAGGAAGAGTCAAATAGTTAATAAGGGAAGGGTTTGGCATGCCCTACGTAAGTTCTACCAGCAAGTCCCAACAAGAAGGCATTCTGTGTCTCCTGATTCCTGACCTACCCCCAAAATGTACAAATGTACAAGGAATGAGCCCACTTTCCCAGCAGGCTGTAATACCAGTTTGGCCTATATCAATGCATTGGTGAGCTGTGTTTTGTTTATGGTTTTATGCCATCTATTTTCCCATGGATATTATGTTTTCTAAAGAGCCCTTAAGTTTACGTCAGCTTTTAAAGCTACCAGCAGCACCATTTCAGTTCATATTAAGCCCTTAATATGGTATGAATAGGAGAGCTATTAGACTAAAGAGCCATAATCATCCCTGAGGAAAACATCCATCACCAACATTTATGTGGTCCCTGAACTTCTAAAAGGTGTCATCTCTCTGGGGTGTATCTGGTGAGAGCTTTCTCTGGGAGATGCCAAAAAGCCAATGCATTAGATGAAGCTTAGAAGGGCATTTTCTAACCATTACAAATTGCCTAGTCTAGCATCTCAATTTCATCTACGTGAAGAGCCTTAATTAAATTTGTTGGGGCTTGATCCTTTATCCCCAGATGTGGCGCTGACAGAGATTGCTTACATAAATAATGTGTGCTCCAAGTGCTTGCCAGGCTCCTGGCTCAGCTGGGACAGCTGTAGCTTTTTGAATGTCATTCCCAAGATATCCTGCAGGTGTTCAGCTTCCCCTGTTCTACTCTGGGAAGAGAGCCGTGGGCAACATCAGCCCAGAAGACACTAGTGCATCTTGACAGAGCATTCCCACTGGACAGGTGCACTACCTGCAGACACCTCCAACAGTGAAAGCACCCCCCGGCTGCAAGTATTGTCTCGCCAAACGGAAGGCATTTCTTGTATGAGTCTTGAATCTGATATTCTTTAATAAGACTTTCTCTAAAACTGTATACACACACGCACCTGAAAACGTTATCAGGCTTTTACTTATTTGACAGTAATATTCCAGGAAATGGTCCAAGTAGCAAAATCTTCTCTCAGTGGCTTTCTCGTTCCTCATGTCTGACTTGCTTTCCTGCTGTCTACTGGAGCCAACAAGAGAGGCATTCTCACTCTGGAAGTTGTTAGTTCATTACAGCCTAGGTTGATCTCATCTGGCTCTGGTTGGGGGTTTTATTCTGAAGATTGGCTGGTAAAAGCCTCCACAAAGTCTTAGCTAGAACATTCATAGCCCTGTGTTGACTTCCGCCTCAACTCTGGCTTGAACTTAGCTGACGGTCCTATCAGTTTGCTCAAAGGAGCTTT

6196-E27enhancer E Tcf4 site2/3 mutant

GGTATGCTAAAGTTTGCAAAACTGGTATAGACACCAAGAGGGAGGTATCAACAGAGACTCCCCAAGAGCTAAGAGGAAACCACCTTGGACTGGAAGTCAAGAGCCAAAATTCTAGACTCTACTCTGTAATTAACTACCTATTTGAATTTGGAAAAATCACCATCAACTTTCCCAGCCTCGTTCTCTGCATCTGGGAAATGAAGGCGTCGTCCAAATGATTACAAGCTTCTTTTCCTGCTCTTATTGCATGATTCCACTTCCACAGCCCTCCAGCATTTTTTAGCAGCTGCATCGCTCCATAGAGCCTGCAGAGGGCACTAGACTGGGAATTAGAAAACCTGATTTCCCTTCCAGCTCCACCTCTGACCAATTGCCTGACCCTGGTCAAATTGCTTAACCTCTTCCTATCTCAGCTCCCTATCCATAAAACAGAGGGACGAATAAACTCTCCTCCTACCACTAAGAGGTGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAGTCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTATTTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAGCCCAAGGGCAGGAACCCAGCAAGTTTCTCAGGATTGGGGCCTTAGACTGGGCTGTGTATACAGACAGTGCCAGCCAACCCCACAGTTCAGTTTCCTTTAACCTGGTGCTCCAGGCAATAACTGTGCAACTCTGCAATTTAACAATGTGTTCTTTGTCCCACAACTGTTCTCGTTTCTCAACTGCCCAGGTAATATGTTTGGGCCTGTAGGAAGAGTCAAATAGTTAATAAGGGAAGGGTTTGGCATGCCCTACGTAAGTTCTACCAGCAAGTCCCAACAAGAAGGCATTCTGTGTCTCCTGATTCCTGACCTACCCCCAAAATGTACAAATGTACAAGGAATGAGCCCACTTTCCCAGCAGGCTGTAATACCAGTTTGGCCTATATCAATGCATTGGTGAGCTGTGTTTTGTTTATGGTTTTATGCCATCTATTTTCCCATGGATATTATGTTTTCTAAAGAGCCCTTAAGTTTACGTCAGCTTTTAAAGCTACCAGCAGCACCATTTCAGTTCATATTAAGCCCTTAATATGGTATGAATAGGAGAGCTATTAGACTAAAGAGCCATAATCATCCCTGAGGAAAACATCCATCACCAACATTTATGTGGTCCCTGAACTTCTAAAAGGTGTCATCTCTCTGGGGTGTATCTGGTGAGAGCTTTCTCTGGGAGATGCCAAAAAGCCAATGCATTAGATGAAGCTTAGAAGGGCATTTTCTAACCATTACAAATTGCCTAGTCTAGCATCTCAATTTCATCTACGTGAAGAGCCTTAATTAAATTTGTTGGGGCTTGATCCTTTATCCCCAGATGTGGCGCTGACAGAGATTGCTTACATAAATAATGTGTGCTCCAAGTGCTTGCCAGGCTCCTGGCTCAGCTGGGACAGCTGTAGCTTTTTGAATGTCATTCCCAAGATATCCTGCAGGTGTTCAGCTTCCCCTGTTCTACTCTGGGAAGAGAGCCGTGGGCAACATCAGCCCAGAAGACACTAGTGCATCTTGACAGAGCATTCCCACTGGACAGGTGCACTACCTGCAGACACCTCCAACAGTGAAAGCACCCCCCGGCTGCAAGTATTGTCTCGCCAAACGGAAGGCATTTCTTGTATGAGTCTTGAATCTGATATTCTTTAATAAGACTTTCTCTAAAACTGTATACACACACGCACCTGAAAACGTTATCAGGCTTTTACTTATTTGACAGTAATATTCCAGGAAATGGTCCAAGTAGCAAAATCTTCTCTCAGTGGCTTTCTCGTTCCTCATGTCTGACTTGCTTTCCTGCTGTCTACTGGAGCCAACAAGAGAGGCATTCTCACTCTGGAAGTTGTTAGTTCATTACAGCCTAGGTTGATCTCATCTGGCTCTGGTTGGGGGTTTTATTCTGAAGATTGGCTGGTAAAAGCCTCCACAAAGTCTTAGCTAGAACATTCATAGCCCTGTGTTGACTTCCGCCTCAACTCTGGCTTGAACTTAGCTGACGGTCCTATCAGTTTGCTCAAAGGAGCTTT

6196-E29control enhancer (E region)

GGATTATAAAGCAAAGATCTATCTAATATATAGATATATCAGAAACTAAAATAAGCCATATCACTGTGTAATTAATTCCCCACCAGCAGACACATTAGAGGAAGCTTTGATGACTACACCCAGATATTATAAGTAGGAGAGTGACTTTTCTGACTTTTAGTACTATAAAACCATTATTCAAATTAAACCATATAGAGAAGACTAAAATATAAAACAGATACAAGGAGAACCACTGTAAGTGTGAGTCTAATTGATGTACCCTCAGACTCTACAGTGGGCCTGAGATATACCACAAGTTATCTAAGACTCTACTTACCATGACTTTAAACTACCAGAAGAGAGAATTTAGGTAGGTTGTGTCTATTAATATACCTTTATCTGAAATTTTCTTCAGTCATCATTCTCCATACCTCTAGCTGACTGTGTCCTCACTCTCCTAAATCCTCTATAGTCTTTGTTCTGTAAACCAGCTTCACTCTAGATAATAAGATAGGTCCTTGAATAAGTGTGCCTGTGTTCAATTTTCTCTATATAGTTTTCATAGTTGATAATGCTAAGAAAATTCCATAACTTTTCAGTCTTTGTTACTTGACATGAAAAGTGGGGATACCAACAACATCTATCACAAGAGATTTTCTTTCAGTGAAGTTTTTGTAAGTTTTTTAATTCAAATGAAGCATTGAGTTAATGCATATGAAACAGTTAGAATAGTGTCTGACATATAGCAAAAGTTTAATAAATATTAGTCATTATTTTTATCAGTCATCAGCAGCTTTTTGGTGTGCAAATAGAAAGAGCTTTGAAAATGGATAGATATGGGTTCAAGTTCAGCACTGCCAATTAATAGCTCTGTGAGCACAGGCAATTTACCTCCAAATTTTTACTTTAATGTCCTCATTATTTGTTCCAAACCCATTGATTGAGCTCCTGCCATGAGCTTGCTACTCTTCCTTGTTCCTTTTGTACAAAATTTGGGTAAAGAGGATTCAAGGAGCTAAAGGATGCAAGTAAATTACTTGGGAGTGAATTGGAGGGTGCCGAAAAAATTAG

6196-E30control enhancer (E C region)

GATGAGATTTTTGACAGATTCTAAGACACTGCTTTTAGACTTGGGCCTTGCCTAGCCCTCTGAAGTCTTCTATCTCATCATCTTTCTTTCAGTTCTCTAAAAGTTTATTCTTCCCTCCTCAGGACCTTTGCCTATATCTTTCCCTCTTCCTAGAATTCAGTCCCAGCTTCCATACTTCTCTCAGGGTATCAATAACTGACTGCCATGTAACATGCCAACCCCAAACTCAATATATTATAACCACAGCTGTCTTATTAGCTCATGGTTCTATGGCCAGCAATTTGGGCTAGGATCAGCAGGATGGTTCTTCTGTTATTGCCTGGAATCACTTTTACAGCTGCAGATATTTGGCATCTGTAGTGGGGCTGAATAATCTAAGATGGCCTCACACAGTCGCTGCAGGCTGTCAACTGGTTGGACTCAGGGGACTCAGCTGCAACAGTTTTTCGCTTTTCTACCAGCCTCTCATCCACTAATAAACTAGACTAGACTAGACTTCTTCAGCTGGCAGTCTCAGAACAATATTCCAAGGGAGCAAGAGCAGAAACTTCCACTTTATCTGTTGGACACTGCAAGTCAAAGGCCAGTCCAGATTTAATGCATGGGGAAAGCAATTCTACCTCTTGGTGGATAAGCTGGTCATCTTTTATATACCACACCTGGCTAACTAACACTGACACTTCAGATGTCAGTTCCCACATCTCTTCTATAGGAGAACATTACTCTGAATCATCAGATATGATAAAATCCTATTATATGCTCTCAAATCACCCTGTTCATTTTCCTTATAGCATTGGTTTCATTTTACGAGCAAATTTTTGTGTGGTTATTTGATTAAATCTGGTTTCCCTCCTGAACTGTGAACTCCATGAGGGCAGGGACAAAGTCTGTATCTTCAGTGTCTACCATAAAGTAGGGCCCAGCATCAGGATTCCATAAGTGCTTGTTGACTGAACACATGAATGTGAAACTAAACTGGGAGGAAAGTGGTTTAGAAAGTTACTCTACCACTTCCTAGTTGTGGGACCTTCATTAAGTTACATAACCACTTTGTTATTCAGCTTTCACATAAGCAAGCTGAGTGTATTCACACATAGTAGGTGCCAATAAATGCCTTTGAAAGCAATATGAGGTTAGTCACTTAATAGTGAATGAAAATTGTATATTTTATCTTAATATATTTTTGGTGCATGAGAAATCACAGAAAAACAGGG

6196-E31enhancer E G allele

GGTATGCTAAAGTTTGCAAAACTGGTATAGACACCAAGAGGGAGGTATCAACAGAGACTCCCCAAGAGCTAAGAGGAAACCACCTTGGACTGGAAGTCAAGAGCCAAAATTCTAGACTCTACTCTGTAATTAACTACCTATTTGAATTTGGAAAAATCACCATCAACTTTCCCAGCCTCGTTCTCTGCATCTGGGAAATGAAGGCGTCGTCCAAATGATTACAAGCTTCTTTTCCTGCTCTTATTGCATGATTCCACTTCCACAGCCCTCCAGCATTTTTTAGCAGCTGCATCGCTCCATAGAGCCTGCAGAGGGCACTAGACTGGGAATTAGAAAACCTGATTTCCCTTCCAGCTCCACCTCTGACCAATTGCCTGACCCTGGTCAAATTGCTTAACCTCTTCCTATCTCAGCTCCCTATCCATAAAACAGAGGGACGAATAAACTCTCCTCCTACCACTAAGAGGTGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAGGCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTATTTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAATCAAAGGGCAGGAACCCAGCAAGTTTCTCAGGATTGGGGCCTTAGACTGGGCTGTGTATACAGACAGTGCCAGCCAACCCCACAGTTCAGTTTCCTTTAACCTGGTGCTCCAGGCAATAACTGTGCAACTCTGCAATTTAACAATGTGTTCTTTGTCCCACAACTGTTCTCGTTTCTCAACTGCCCAGGTAATATGTTTGGGCCTGTAGGAAGAGTCAAATAGTTAATAAGGGAAGGGTTTGGCATGCCCTACGTAAGTTCTACCAGCAAGTCCCAACAAGAAGGCATTCTGTGTCTCCTGATTCCTGACCTACCCCCAAAATGTACAAATGTACAAGGAATGAGCCCACTTTCCCAGCAGGCTGTAATACCAGTTTGGCCTATATCAATGCATTGGTGAGCTGTGTTTTGTTTATGGTTTTATGCCATCTATTTTCCCATGGATATTATGTTTTCTAAAGAGCCCTTAAGTTTACGTCAGCTTTTAAAGCTACCAGCAGCACCATTTCAGTTCATATTAAGCCCTTAATATGGTATGAATAGGAGAGCTATTAGACTAAAGAGCCATAATCATCCCTGAGGAAAACATCCATCACCAACATTTATGTGGTCCCTGAACTTCTAAAAGGTGTCATCTCTCTGGGGTGTATCTGGTGAGAGCTTTCTCTGGGAGATGCCAAAAAGCCAATGCATTAGATGAAGCTTAGAAGGGCATTTTCTAACCATTACAAATTGCCTAGTCTAGCATCTCAATTTCATCTACGTGAAGAGCCTTAATTAAATTTGTTGGGGTTTGATCCTTTATCCCCAGATGTGGCGCTGACAGAGATTGCTTACATAAATAATGTGTGCTCCAAGTGCTTGCCAGGCTCCTGGCTCAGCTGGGACAGCTGTAGCTTTTTGAATGTCATTCCCAAGATATCCTGCAGGTGTTCAGCTTCCCCTGTTCTACTCTGGGAAGAGAGCCGTGGGCAACATCAGCCCAGAAGACACTAGTGCATCTTGACAGAGCATTCCCACTGGACAGGTGCACTACCTGCAGACACCTCCAACAGTGAAAGCACCCCCCGGCTGCAAGTATTGTCTCGCCAAACGGAAGGCATTTCTTGTATGAGTCTTGAATCTGATATTCTTTAATAAGACTTTCTCTAAAACTGTATACACACACGCACCTGAAAACGTTATCAGGCTTTTACTTATTTGACAGTAATATTCCAGGAAATGGTCCAAGTAGCAAAATCTTCTCTCAGTGGCTTTCTCGTTCCTCATGTCTGACTTGCTTTCCTGCTGTCTACTGGAGCCAACAAGAGAGGCATTCTCACTCTGGAAGTTGTTAGTTCATTACAGCCTAGGTTGATCTCATCTGGCTCTGGTTGGGGGTTTTATTCTGAAGATTGGCTGGTAAAAGCCTCCACAAAGTCTTAGCTAGAACATTCATAGCCCTGTGTTGACTTCCGCCTCAACTCTGGCTTGAACTTAGCTGACGGTCCTATCAGTTTGCTCAAAGGAGCTTT

pDest-302 backbone

(Enhancer)CAACTTTGTACAAAAAAGTTGGC(Promoter/Reporter)CAACTTTCTTGTACAAAGTTGATAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGAAGCTTGAATTCACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTATCAACTTTGTATAGAAAAGTTGGC

Oligonucleotide Primers (in 5’-3’ orientation)

pTK start / GGGGACAACTTTGTACAAAAAAGTTGGCCTAAATGAGTCTTCGGACCTCGCGGGGG
pMyc start / GGGGACAACTTTGTATAGAAAAGTTGGCCAGCTGTTCCGCCTGCGATGATTTATAC
ffLuc2-eGFP end / GGGGACAACTTTGTACAAGAAAGTTGACTCGAGTTACTACTTGTACAGCTCGTCCATG
control enh start / GGGGACAACTTTGTATAGAAAAGTTGGCTAATAAGAGAAAAAAATCACTGGATATACC
control enh end / GGGGCCAACTTTTTTGTACAAAGTTGCTACGCCCCGCCCTGCCACTCATCGCAG
Enhancer D start / GGGGACAACTTTGTATAGAAAAGTTGGCTGAAGCCAATAACCTGGTGAATAATTC
Enhancer D end / GGGGCCAACTTTTTTGTACAAAGTTGTAAAAGGCCAACAGGAACCACATCTCCGGC
Enhancer G start / GGGGACAACTTTGTATAGAAAAGTTGGCGAATTTGACCAAAGAAGCCAATAG
Enhancer G end / GGGGCCAACTTTTTTGTACAAAGTTGGGGGTTCTCATGTAGCAAACAGTTTTGTGG
Enhancer E start / GGGGACAACTTTGTATAGAAAAGTTGGCGGTATGCTAAAGTTTGCAAAACTGG
Enhancer E end / GGGGCCAACTTTTTTGTACAAAGTTGAAAGCTCCTTTGAGCAAACTGATAGGACCG
Enhancer F start / GGGGACAACTTTGTATAGAAAAGTTGGCCTCCAGTGTAAGAATGGTGACAC
Enhancer F end / GGGGCCAACTTTTTTGTACAAAGTTGAATACATTTAACTTAATTATTCTGAGCATTCAG
Enhancer B start / GGGGACAACTTTGTATAGAAAAGTTGGCATAATATTGTTAATAAAATAACCCAGCCAG
Enhancer B end / GGGGCCAACTTTTTTGTACAAAGTTGTCTCTATCACAAACTAACTATCCTGTCC
Enhancer A start / GGGGACAACTTTGTATAGAAAAGTTGGCGTCCTGGTGTCTTTCTCATGCTCTTTG
Enhancer A end / GGGGCCAACTTTTTTGTACAAAGTTGCCTGAATGTGCTTGACCAATGGACACAGC
Enhancer C start / GGGGACAACTTTGTATAGAAAAGTTGGCCAGGAGGTGGGACAGATAAATCACAGTTTGTG
Enhancer C end / GGGGCCAACTTTTTTGTACAAAGTTGAAACTGTGTGTCCCTCTCTGGACCTCAAC
CMVenh start / GGGGACAACTTTGTATAGAAAAGTTGGCAGTTCATAGCCCATATATGGAGTTCCGCG
CMVenh end / GGGGCCAACTTTTTTGTACAAAGTTGTCAATGGGGCGGAGTTGTTACGAC
controlE start / GGGGACAACTTTGTATAGAAAAGTTGGCGGATTATAAAGCAAAGATCTATC
controlE end / GGGGCCAACTTTTTTGTACAAAGTTGCTAATTTTTTCGGCACCCTCC
controlC start / GGGGACAACTTTGTATAGAAAAGTTGGCGATGAGATTTTTGACAGATTC
controlC end / GGGGCCAACTTTTTTGTACAAAGTTGCCCTGTTTTTCTGTGATTTCTCATGCACC

Evolutionary conservation of SNP rs6983267:

Chimp

450 . : . : . : . : . :
aa.g 1 M K G
+8:126997917 TGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAG
||||||||||||||||||||||||||||||||||||||||||||||||||
451 TGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAG
aa.c 1 M K X
500 . : . : . : . : . :
aa.g 4 T E K S T K N F Y V L L T L F Y
+8:126997967 GCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTAT
|||||||||||||||||||||||||||||||||||||||||||||||||
501 KCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTAT
aa.c 4 T E K S T K N F Y V L L T L F Y
550 . : . : . : . : . :
aa.g 20 F M W G R E P A P A G K L L S L N
+8:126998017 TTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAA
||||||||||||||||||||||||||||||||||||||||||||||||||
551 TTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAA
aa.c 20 F M W G R E P A P A G K L L S L N
Macaque:
450 . : . : . : . : . :
aa.g 1 M K G
+8:130014195 TGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAG
||||||||||||||||||||||||||||||||||||||||||||||||||
451 TGTAGCCAGAGTTAATACCCTCATCGTCCTTTGAGCTCAGCAGATGAAAG
aa.c 1 M K X
500 . : . : . : . : . :
aa.g 4 T E K S T K N F Y V L L T L F Y
+8:130014245 GCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTAT
|||||||||||||||||||||||||||||||||||||||||||||||||
501 KCACTGAGAAAAGTACAAAGAATTTTTATGTGCTATTGACTTTATTTTAT
aa.c 4 T E K S T K N F Y V L L T L F Y
550 . : . : . : . : . :
aa.g 20 F M W G R E S A P A G K L L S L N
+8:130014295 TTTATGTGGGGGAGGGAGTCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAA
|||||||||||||||||| |||||||||||||||||||||||||||||||
551 TTTATGTGGGGGAGGGAGCCGGCCCCAGCTGGAAAGCTGCTTTCTCTGAA
aa.c 20 F M W G R E P A P A G K L L S L N

Supplemental Figure. 1. Effect of CMV Enhancer Element over Myc and TK Promoters. The CMV enhancer was cloned in front of a Myc promoter-driven or TK promoter-driven luciferase-GFP reporter. Luciferase activity was recorded in prostate cell lines LnCAP, and PC3 as well as colorectal cell line SW620. Enhancer reporter plasmids were co-transfected into cell lines as described in materials and methods. Luciferase activity was measured 24 hrs post-transfection using the Dual-Glo Luciferae assay system.

Supplemental Figure 2. Enhancer E interacts with Tcf-4 in vivo. A. -catenin/Tcf-4 can stimulate enhancer E activity in the luciferase reporter assay. Luciferase activity of Myc-luciferase constructs (with and without enhancer E (eE)) in prostate cell line LnCAP with/without (+/-) -catenin and/or Tcf-4.

Supplemental table 1, 2, and 3. SNP rs6893267 influences enhancer E activity.

Luciferase activity of enhancer-Myc-luciferase constructs in prostate cell line LnCAP with or without -catenin /Tcf-4 (25ng of each plasmid). The G and T allele variants of enhancer E are indicated.