Table SIII. Mean levels of all 38unknown metabolites (natural scale)significant(Sig) for time, ordered by significance from an ANOVA F-test. Values are expressed in units of response ratio to internal standard.

Metabolites increasing over time / Time (T) / Avg.
SED / Sig / Technology
T0 / T1 / T2
U0265153_0883 / 0.180 / 1.031 / 3.869 / 0.1497 / *** / LC-MS
U0235143_0781 / 0.001 / 0.022 / 0.068 / 0.0050 / *** / LC-MS
U0695361_1080 / 0.058 / 0.070 / 0.176 / 0.0113 / *** / LC-MS
U0638303_1268 / 0.028 / 0.044 / 0.086 / 0.0053 / *** / LC-MS
U1895 / 0.272 / 0.501 / 0.611 / 0.0307 / *** / GC-MS NP
U0595163_1209 / 0.001 / 0.002 / 0.017 / 0.0018 / *** / LC-MS
U0367243_2163 / 0.007 / 0.012 / 0.019 / 0.0012 / *** / LC-MS
U0472242_0959 / 0.006 / 0.009 / 0.013 / 0.0007 / *** / LC-MS
U0295225_2965 / 0.174 / 0.284 / 0.451 / 0.0286 / *** / LC-MS
U0365227_2075 / 0.002 / 0.006 / 0.013 / 0.0011 / *** / LC-MS
U0381077_0187 / 0.257 / 0.400 / 0.439 / 0.0216 / *** / LC-MS
U2466 / 0.573 / 1.061 / 1.396 / 0.0951 / *** / GC-MS NP
U1953 / 0.019 / 0.020 / 0.029 / 0.0014 / *** / GC-MS P
U0474257_0903 / 0.407 / 0.483 / 0.752 / 0.0471 / *** / LC-MS
U0379243_2803 / 0.000 / 0.001 / 0.001 / 0.0001 / *** / LC-MS
U0346257_1672 / 0.004 / 0.011 / 0.025 / 0.0030 / *** / LC-MS
U0348272_1780 / 0.029 / 0.044 / 0.076 / 0.0073 / *** / LC-MS
U0531315_0735 / 0.038 / 0.043 / 0.061 / 0.0039 / *** / LC-MS
U2457 / 0.079 / 0.208 / 0.369 / 0.0605 / ** / GC-MS NP
U1845 / 0.069 / 0.137 / 0.224 / 0.0356 / ** / GC-MS NP
U2510 / 0.309 / 0.666 / 1.801 / 0.3893 / ** / GC-MS NP
U0381259_2617 / 0.001 / 0.002 / 0.007 / 0.0017 / * / LC-MS
U2190 / 0.005 / 0.009 / 0.010 / 0.0018 / * / GC-MS P
U1762 / 0.034 / 0.081 / 0.106 / 0.0251 / * / GC-MS NP
Metabolites decreasing over time / Time (T) / Avg.
SED / Sig / Technology
T0 / T1 / T2
U0598322_2371 / 0.001 / 0.000 / 0.000 / 0.0000 / *** / LC-MS
U0696413_2357 / 0.007 / 0.006 / 0.004 / 0.0005 / *** / LC-MS
U1598 / 0.185 / 0.154 / 0.145 / 0.0087 / ** / GC-MS P
U0464156_1171 / 0.009 / 0.008 / 0.007 / 0.0005 / ** / LC-MS
U0597300_2248 / 0.030 / 0.024 / 0.020 / 0.0023 / ** / LC-MS
U0536331_1961 / 0.001 / 0.001 / 0.000 / 0.0002 / * / LC-MS
U1585 / 0.100 / 0.079 / 0.068 / 0.0109 / * / GC-MS P
Metabolites with ‘alternative’
patterns over time / Time (T) / Avg.
SED / Sig / Technology
T0 / T1 / T2
U0298095_0692 / 0.039 / 0.163 / 0.145 / 0.0095 / *** / LC-MS
U0361196_2580 / 0.001 / 0.002 / 0.001 / 0.0002 / *** / LC-MS
U0314137_1544 / 0.027 / 0.060 / 0.055 / 0.0051 / *** / LC-MS
U0694397_2218 / 0.002 / 0.005 / 0.006 / 0.0008 / *** / LC-MS
U0392995_0176 / 0.016 / 0.016 / 0.014 / 0.0006 / ** / LC-MS
U1567 / 0.028 / 0.020 / 0.020 / 0.0029 / * / GC-MS P
U1816 / 0.005 / 0.003 / 0.004 / 0.0006 / * / GC-MS P

ANOVA = Analysis of Variance; Avg. SED = Average Standard Error of Difference; Sig = Significance.

* = significant (p < 0.05); ** = significant (p < 0.01); *** = significant (p < 0.001).

GC-MS = Gas Chromatography-Mass Spectrometry; GM = genetically modified; LC-MS = Liquid Chromatography-Mass Spectrometry; NP = non-polar; P = polar;T = time; T0 = Time-point 0 (0 hours); T1 = Time-point 1 (24 hours); T2 = Time-point 2 (48 hours); U = unknown; WT = wild-type.