1. Antibody Specific Caspase 3 Detection Kits
Cell Technology would like to inform you about the promotion on our Apo
Active Caspase 3 Detection Kits. Catalog #'s FAB200-2 and PAB200-2. Save $20
off the regular price in June 2006.
Key Benefits:
* Highly specific for active human and murine caspase 3. Other assays
require the utilization of peptide based (DEVD) reagents that tend to
cross-react with caspase 7 and other caspases.
* Works with a flow cytometer, flourescence microscope, or 96 well plate
reader.
* Yields both quantitative and qualitative results. Gives a strong positive
signal.
* Can be used in conjunction with other antibodies or stains.
* No need to make cell lysates or run western blots. Cells can be fixed and
analyzed later.
* Works with human, mouse and rat cell lines.
Click http://celltechnology.com/products/June06.shtml to learn more.
Assay Principle
Cell Technology ’s APO-ACTIVE and APO ACTIVE 3PE CASPASE 3 Kits utilize a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC / PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.
Citations
Deficiency of the Cyclin Kinase Inhibitor p21(WAF-1/CIP-1) Promotes
Apoptosis of Activated/Memory T Cells and Inhibits Spontaneous Systemic
Autoimmunity - Lawson, Baccala, Song, et al - JEM, Volume 199, Number 4,
547-557
References
1. Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers:
orderingcaspase activation events in apoptosis. Cell Death and Differ.
6:1067-1074.
2. Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C.
R.Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994.
Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme:
A(p20/p10)2 Homodimer. Cell 78:343-352.
3. Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M.
A.Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D.
J.Livingston. 1994. Structure and mechanism of interleukin-1 beta converting
enzyme. Nature 370: 270-275.
4. Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M.
Labelle,E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A.
Thornberry and J.W. Becker. 1996. The three-dimensional structure of
apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7):
619-625.
5. Kumar, S. 1999. Mechanisms mediating caspase activation in cell death.
Cell Death and Differ. 6: 1060-1066.
6. Alnemri, E.S. et al (1996) Cell 87:171
7. Trends Biochem Sci 22,388 (1997)
8. Nature 376, 37 (1995).
If you would like any other information about our products or have any
questions, please feel free to contact us at
or (650)-960-2170 .
Thank you,
Lalitha Ramachandran
Sales Representative
www.celltechnology.com
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subject header.
2. HybriProbe Custom Design Kits
> Boca Scientific Inc <> 6/14/2006 12:30 PM >
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HybriProbe Custom Design Kits
for non-radioactive in situ hybridization
In situ hybridization (ISH) is a powerful technique for visualizing
and localizing the expression of specific mRNAs in cells and tissue
sections.
Biognostik has developed a unique technology to design and manufacture
premium quality hybridization probes specific to any target mRNA of
interest. These premium quality HybriProbes are offered as convenient
ready-to-use HybriProbe Custom Design Kits.
Thanks to their sequence design, optimized length and high purity
standard, Hybriprobes feature excellent tissue penetration, high
specificity and low background.
All HybriProbes are designed to work under similar hybridization and
washing conditions. This greatly facilitates parallel detection of
different genes.
http://www.bocascientific.com/product_info.php?cPath=150&products_id=1553
HybriProbe Custom Design TriSeq-Kits include the following items:
Three specific HybriProbesSimultaneous use of three different
Hybriprobes against different regions of the same target mRNA enhances
signal intensity and assay sensitivity
Three random control HybriProbes (negative control)
Poly d(T) control (positive control)
Beta-actin control (positive control)
Alpha-tubulin control (positive control)
HybriBuffer-ISH
All HybriProbes are double-FITC-labelledHybriProbe Kits are compatible
with NBT/BCIP or DAB-based FITC detection systems available from well
known manufacturers
Detailed HybriProbe Manual: All steps from tissue preparation to
detection
Professional technical customer support by Biognostiḱs application
specialists
http://www.bocascientific.com/docs/HybriProbe.pdf
Click here to download the HybriProbe Order Form
For the design process, please provide name and species of your target
gene and the corresponding GenBank Accession Number of the mRNA or
cDNA.
http://www.bocascientific.com/product_info.php?cPath=150&products_id=1553
Click here for Ordering Information
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