Devos Y & De Schrijver A, De Clercq P, Kiss J, Romeis J (2012) Bt-maize event MON88017 expressing Cry3Bb1 does not cause harm to non-target organisms. Transgenic Research, DOI:10.1007/s11248-012-9617-z
Online Supporting Material
TableS1.Overview of lower-tier studies investigating potential adverse effects of purified Cry3Bb1 protein or Cry3Bb1-containing food of vegetal or animal origin on non-target terrestrial (plant- and ground-dwelling) arthropods
TableS2.Overview of higher-tier studies investigating potential adverse effects of the Cry3Bb1-expressing maize events on non-target terrestrial (plant- and ground-dwelling) arthropods
TableS3.Overview of studies investigating potential adverse effects of purified Cry3Bb1 protein or Cry3Bb1-containing food of vegetal origin on non-target soil arthropods
TableS4.Overview of studies investigating potential adverse effects of purified Cry3Bb1 protein or Cry3Bb1-containing food of vegetal origin on non-target aquatic arthropods
TableS5.Overview of studies investigating potential adverse effects of purified Cry3Bb1 proteinor Cry3Bb1-containing food of vegetal origin on Annelida, Mollusca and Nematoda
TableS1.Overview of lower-tier studies investigating potential adverse effects of purified Cry3Bb1 protein or Cry3Bb1-containing food of vegetal or animal origin on non-target terrestrial (plant- and ground-dwelling) arthropods
Order: Family / Functional group (feeding guild)* / Stage tested / Effect reported / Type of study / Test material / Reference(see Devos et al. (2012) for reference list)
Species
Acari:Tetranychidae
Tetranychus urticae / Herbivore / Larva & adult / No effect on survival, pre-oviposition period, fecundity, fertility and survival and development time of offspring / Tier 1b / MON88017 leaf material / Li and Romeis (2010)
Araneae: Theridiidae
Phylloneta impressa (formerly named asTheridion impressum) / Predator / Juvenile / No effect on survival and growth / Tier 1b / MON88017 pollen (10mg) or prey (Tetranychusurticae) fed MON88017 material / Meissle and Romeis (2009b)
Adult / No effect on survival, weight and fertility / Tier 1b / Prey (Chrysoperla carnea, WCR) fed MON88017 material / Meissle and Romeis (2009b)
Coleoptera: Carabidae
Agonum cupripenne / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Agonum placidum / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Amara pennsylvanicus / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Calathus ambiguus / Predator / Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 325g/ml diet / Priesnitz (2010)
Calathus fuscipes / Predator / Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 325g/ml diet / Priesnitz (2010)
Chlaenius tricolor / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Harpalus affinis / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Harpalus caliginosus / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-50mg depending on beetle size / Mullin et al. (2005)
Adult / No effect on survival, fecundity and fertility / Tier 1b / MON863 pollen; 1g / Ahmad et al. (2006a)
Harpalus pennsylvanicus / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Adult / No effect on survival / Tier 1b / MON863 pollen; 1g or 1:10 ratio depending on diet / Ahmad et al. (2006a)
Patrobus longicornis / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Poecilus chalcites / Predator / Larva / No effect on survival, development rate and growth / Tier 1a / Cry3Bb1 protein; 930g/g diet / Duan et al. (2006a)
Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Poecilus lucublandus / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Pseudophonus rufipes / Predator / Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 325g/ml diet / Priesnitz (2010)
Pterostichus melanarius / Predator / Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 325g/ml diet / Priesnitz (2010)
Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Scarites quadriceps / Predator / Adult / No effect on survival time / Tier 1b / MON863 pollen; 5-25mg depending on beetle size / Mullin et al. (2005)
Coleoptera: Chrysomelidae
Callosobruchus maculatus / Herbivore / Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; up to 200ppm / Head et al. (2001)
Galerucella vittaticollis / Herbivore / Larva / No effect on survival and development rate / Tier 1b / MON863 pollen; 500, 1,000 and 2,000pollen grains/cm2 leaf / Shirai (2006)
Gastrophysa viridula / Herbivore / Larva / Decreased survival, development rate and weight / Tier 1a / Cry3Bb1 protein; up to 0.68g / Huber and Langenbruch (2008)
Larva / No effect on survival, development rate and weight / Tier 1b / MON88017 pollen; 470 and 750pollen grains / Huber and Langenbruch (2008)
Leptinotarsa decemlineata / Herbivore / Larva / Decreased survival / Tier 1a / Cry3B protein / Donovan et al. (1992)
Larva / Decreased survival and growth / Tier 1a / Cry3Bb1 protein; up to 0.68g / Huber and Langenbruch (2008)
Larva / Decreased survival and growth / Tier 1b / MON88017 silk, pollen, roots or leaves / Meissle and Romeis (2009a)
Larva / No effect on survival, development rate and weight / Tier 1b / MON88017 pollen; 470 and 750pollen grains / Huber and Langenbruch (2008)
Oulema melanopus / Herbivore / Larva / Decreased survival; no effect on developmental time and weight / Tier 1b / MON88017 leaf material / Meissle et al. (2012)
Adult / No effect on survival and weight / Tier 1b / MON88017 leaf material / Meissle et al. (2012)
Phaedon cochleariae / Herbivore / Larva / Decreased survival and weight; no effect on development rate / Tier 1a / Cry3Bb1 protein; up to 0.68g / Huber and Langenbruch (2008)
Larva / No effect on survival, development rate and weight / Tier 1b / MON88017 pollen; 470 and 750 pollen grains / Huber and Langenbruch (2008)
Coleoptera: Coccinellidae
Adalia bipunctata / Predator / Larva / Decreased survival of 1st instars at 25g/ml; no effect at other (including higher) doses and on later instars, development time and weight / Tier 1a / Cry3Bb protein; up to50g/ml solution / Schmidt et al. (2009)
Larva / No effect on survival, development time and weight / Tier 1a / Cry3Bb1 protein; 200g/ml solution / Álvarez-Alfageme et al. (2011)
Larva / No effect on survival, development time and weight / Tier 1b / Prey (T. urticae) fed MON88017 material / Álvarez-Alfageme et al. (2011)
Coleomegilla maculata / Predator / Larva / No effect on survival, development time and weight / Tier 1b / MON863 pollen; 50%of diet (w/w) / Duan et al. (2001a)
Adult / No effect on survival / Tier 1b / MON863 pollen;50% of diet (w/w) / Duan et al. (2001b)
Larva / No effect on survival, development time and weight / Tier 1b / MON863 pollen; 50%of diet (w/w) / Duan et al. (2002)
Adult / No effect on survival, weight and fecundity / Tier 1b / MON863 pollen; 50% of diet (w/w) / Duan et al. (2002)
Larva / No effect on survival, development time, weight, pre-oviposition period and mobility / Tier 1b / MON863 pollen; diet containing 25, 50, 75 and 100% pollen / Lundgren and Wiedenmann (2002)
Larva / No effect on survival, development time, weight, elytra length and longevity / Tier 1b / MON863 pollen; ad libitum / Ahmad et al. (2006a)
Larva / No effect on survival, development time, weight, fertility and mobility / Tier 1b / Prey (Rhopalosiphum maidis and R.padi) fed MON863 material / Lundgren and Wiedenmann (2005)
Epilachna vigintioctopunctata / Herbivore / Larva / No effect on survival and development rate / Tier 1b / MON863 pollen;250, 500, 1,000 and 2,000 pollen grains/cm2 leaf / Shirai (2006)
Hippodamia convergens / Predator / Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 400 and 8,000g/ml diet / Palmer and Krueger (1999a)
Adult / No effect on survival / Tier 1b / MON863 pollen; 50% of diet (w/w) / Bryan et al. (2001)
Stethorus punctillum / Predator / Larva / No effect on survival, development time and weight, but female beetles had a shorter pre-oviposition period,increased fecundity andhigher fertility in Bt-treatment / Tier 1b / Prey (T. urticae) fed MON88017 material / Li and Romeis (2010)
Coleoptera: Curculionidae
Anthonomus eugenii / Herbivore / Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; up to 200g/ml diet / Head et al. (2001)
Anthonomus grandis / Herbivore / Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; up to 50g/ml diet / Head et al. (2001)
Sitophilus oryzae / Herbivore / Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; up to 200g/ml diet / Head et al. (2001)
Coleoptera: Staphylinidae
Atheta coriaria / Predator / Larvae / No effect on survival, development time, growth andpredatory ability / Tier 1b / Prey (T. urticae) fed MON88017 material / García et al. (2011)
Adult / No effect on survival, weight, head capsule width, elytra length, pre-oviposition period, fecundity, fertility andpredatory ability / Tier 1b / Prey (T. urticae) fed MON88017 material / García et al. (2011)
Coleoptera: Tenebrionidae
Tribolium castaneum / Herbivore / Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; up to 200g/ml diet / Head et al. (2001)
Diptera: Drosophilidae
Drosophila melanogaster / Decomposer (detritivore) / Larva / No effects on development time and fertility / Tier 1b / MON88017 leaf material / Knecht and Nentwig (2010)
Diptera: Phoridae
Megaselia scalaris / Decomposer (detritivore) / Larva / No effects on development time and fertility / Tier 1b / MON88017 leaf material / Knecht and Nentwig (2010)
Hemiptera: Anthocoridae
Orius insidiosus / Predator / Nymph / No effect on survival and development rate / Tier 1a / Cry3Bb1 protein; 930g/g diet / Duan et al. (2008b)
Hemiptera: Aphidae
Rhopalosiphum maidis / Herbivore / Nymph & adult / Plant variety effect on weight / Tier 1b / MON863 leaf material / Lundgren and Wiedenmann (2005)
Rhopalosiphum padi / Herbivore / Nymph & adult / Plant variety effect on weight / Tier 1b / MON863 leaf material / Lundgren and Wiedenmann (2005)
Hymenoptera: Pteromalidae
Nasonia vitripennis / Parasitoid / Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 400 and 8,000g/ml-containing Cry3Bb1 diet / Palmer and Krueger (1999c)
Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 1,860g/ml diet / Sindermann et al. (2002b)
Hymenoptera: Apidae
Apis mellifera / Pollinator / Larva / No effects on survival and weight / Tier 1a / Cry3B protein; 0.066 and 0.332% Cry3B-containing diet / Arpaia (1996)
Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; 1,790g/ml / Maggi (1999a)
Adult / No effect on survival / Tier 1a / Cry3Bb1 protein; 360g/ml diet / Maggi (1999b)
Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; 2,550g/ml / Maggi (2002)
Larva / No effect on survival and development time / Tier 1b / MON88017 pollen; 2mg / Richards (2011a)
Adult / No effect on survival / Tier 1b / MON88017 pollen; 4g/ml diet / Richards (2011b)
Larva / No effect on survival and weight / Tier 1b / MON89034 x MON88017 pollen; 2mg / Hendriksma et al. (2011)
Larva / No effect on survival and weight / Tier 1a / Cry3Bb1 protein; up to 3g/10l diet / Hendriksma et al. (2012)
Lepidoptera: Crambidae
Ostrinia nubilalis / Herbivore / Larva / No effect on survival and weight / Tier 1a / Cry3Bb1 protein; 200g/ml diet / Head et al. (2001)
Lepidoptera: Danaidae
Danaus plexippus / Herbivore / Larva / No effect on survival, development rate, growth and consumption / Tier 1b / MON863 pollen; up to 3,200pollen grains/cm2 leaf / Sears and Mattila (2001); Mattila et al. (2005)
Lepidoptera: Noctuidae
Helicoverpa zea / Herbivore / Larva / No effect on survival and weight / Tier 1a / Cry3Bb1 protein; 200g/ml diet / Head et al. (2001)
Neuroptera: Chrysopidae
Chrysoperla carnea / Predator / Larva / No effect on survival / Tier 1a / Cry3Bb1 protein; 400 and 8,000g/g diet / Palmer and Krueger (1999b)
Adult / No effect on survival, weight, pre-oviposition period, fecundity and fertility / Tier 1a / Cry3Bb1 protein; 150g/g diet / Li et al. (2008)
Adult / No effect on survival, weight, pre-oviposition period, fecundity and fertility / Tier 1b / MON88017 pollen; ad libitum / Li et al. (2008)
* Functional group is the primary functional group for which a species is valued
Tier1a refers to in vitro studies carried out with purified Cry3Bb1 protein under laboratory conditions
Tier1b refers to in planta testing using bi- or multi-trophic experiments under laboratory conditions
TableS2.Overview of higher-tier studies investigating potential adverse effects of the Cry3Bb1-expressing maize events on non-target terrestrial (plant- and ground-dwelling) arthropods
Order: Family / Functional group (feeding guild)* / Geographical region where higher-tier study was conducted / Effect reported / Type of study / Test material / Reference(see Devos et al. (2012) for reference list)
Species
Araneae
Various species such as:
Oedothorax apicatus;
Pardosa agrestis;
Pachygnatha degeeri / Predator / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Illinois (USA) / No effect of Bt-maize or seed coat on abundance; decreased abundance after application of soil or foliar insecticide / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a,b)
Chilopoda
Various species / Decomposer (detritivore) / Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Coleoptera: Carabidae
Various species such as: Amara aenea;
Amara similata;
Bembidion lampros;
Bembidion quadrimaculatum;
Calathus ambiguus;
Calathus fuscipes;
Calathus melanocephalus;
Harpalus aeneus;
Harpalus distinguendes;
Platynus dorsalis;
Poecilus cupreus;
Pseudophonus rufipes;
Pterostichus melanarius;
Trechus quadristriatus / Predator / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Illinois (USA) / No effect of Bt-maize or seed coat on abundance; decreased abundance after application of soil insecticide / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Germany (EU) / No effect of Bt-maize on abundance and diversity / Tier 3 / MON88017 / Priesnitz (2010)
Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a)
Coleoptera: Chrysomelidae
Various species such as:
Chaetocnema pulicaria;
Oulema lichenis;
Phyllotreta vittula / Herbivore / Illinois (USA) / Decreased abundance (target group) in Bt-maize / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Germany (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Rauschen et al. (2010a)
Coleoptera: Cicindelidae
Various species / Predator / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Coleoptera: Coccinellidae
Various species such as:
Adalia bipunctata;
Coccinella quinquepunctata;
Coccinella septempunctata;
Coleomegilla maculata;
Harmonia axyridis;
Hippodamia convergens;
Propylea quatuordecimpunctata;
Psyllobora vigintiduopunctata;
Scymnus spp. / Predator / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863, MON853 / Al-Deeb and Wilde (2003)
Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance; decreased abundance after application of foliar insecticide / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
South Dakota (USA) / No effect or slightly increased abundance in Bt-maize compared to near-isogenic line; no effect of soil insecticide / Tier 3 / Cry3Bb1-expressing maize / McManus et al. (2005)
Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance and egg predation / Tier 3 / MON863 / Ahmad et al. (2006a)
Germany (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Rauschen et al. (2010a)
Slovakia (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017, MON89034, MON89034 x MON88017 / Rosca and Cagan (2012a)
Coleoptera: Elateridae
Various species / Herbivore / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Coleoptera: Lathridiidae
Various species such as:
Cortinicara gibbosa / Decomposer (fungivore, detritivore) / Illinois (USA) / No effect of Bt-maize or seed coat on abundance / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Germany (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Rauschen et al. (2010a)
Coleoptera: Nitidulidae
Various species / Decomposer (detritivore) / Illinois (USA) / No effect of Bt-maize or seed coat on abundance / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Coleoptera: Staphylinidae
Various species such as:
Aloconota sulcifrons;
Amischa analis;
Anotylus rugosus;
Mycetoporus eppelsheimianus;
Tachyporus hypnorum / Predator / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Illinois (USA) / No effect of Bt-maize or seed coat on abundance / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Switzerland / No effect of Bt-maize on abundance in litter bags; no differences among conventional maize varieties / Tier 2 / MON88017 leaf material / Höneman et al. (2008)
Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a)
Diplura: Japygidae
Various species / Decomposer (detritivore) / Illinois (USA) / No effect of Bt-maize or seed coat on abundance / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Diptera
Various species / Decomposer (detritivore) / - / No effect of Bt-maize on abundance in litter bags; no differences among conventional maize varieties / Tier 2 / MON88017 leaf material / Höneman et al. (2008)
Diptera: Syrphidae
Various species such as:
Episyrphus balteatus / Predator / Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticides on abundance / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a)
Hemiptera: Anthocoridae
Various species such as: Orius insidiosus / Predator / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863, MON853 / Al-Deeb and Wilde (2003)
Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance and egg predation / Tier 3 / MON863 / Ahmad et al. (2006a)
Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a)
Hemiptera: Aphididae
Various species such as: Metopolophium dirhodum;
Rhopalosiphum maidis;
Rhopalosiphum padi / Herbivore (phloem sap feeder) / - / Decreased weight on Bt-maize / Tier 2 / MON863 / Lundgren and Wiedenmann (2005)
Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance; increased abundance after application of foliar insecticide / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a)
Hemiptera: Cicadellidae
Zyginidia scutellaris / Herbivore / Germany (EU) / No effect of Bt-maize on abundance, but differences among conventional maize varieties / Tier 3 / MON88017 / Rauschen et al. (2008, 2010b)
Hemiptera: Miridae
Trigonotylus caelestialium / Herbivore / Germany (EU) / No effect of Bt-maize on abundance, but differences among conventional maize varieties / Tier 3 / MON88017 / Rauschen et al. (2008, 2009)
Hemiptera: Nabidae
Various species / Predator / Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance; decreased abundance after application of foliar insecticide / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Hymenoptera: Braconidae
Various species such as: Macrocentrus cingulum / Parasitoid / Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Hymenoptera: Formicidae
Various species / Predator (omnivore) / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Mecoptera: Panorpidae
Various species such as:
Panorpa communis / Decomposer (detritivore) / Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a)
Neuroptera: Chrysopidae
Chrysoperla carnea / Predator / Illinois (USA) / No effect on abundance of Bt-maize, seed coat or soil insecticide; decreased abundance after application of foliar insecticide / Tier 3 / MON863 / Bhatti et al. (2005b); see also Duan et al. (2006b)
Slovakia (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017, MON89034, MON89034 x MON88017 / Rosca and Cagan (2012b)
Neuroptera: Osmylidae
Osmylus fulvicephalus / Predator / Slovakia (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017, MON89034, MON89034 x MON88017 / Rosca and Cagan (2012b)
Orthoptera: Gryllidae
Various species / Herbivore / Kansas (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Illinois (USA) / No effect of Bt-maize, seed coat or soil insecticide on abundance / Tier 3 / MON863 / Bhatti et al. (2005a); see also Duan et al. (2006b)
Thysanoptera: Thripidae
Various species such as:
Frankliniella occidentalis / Herbivore / Czech Republic (EU) / No effect of Bt-maize on abundance / Tier 3 / MON88017 / Svobodová et al. (2012a)
* Functional group is the primary functional group for which a species is valued
Tier2 refers to semi-field studies (e.g., caged plants, screen houses)
Tier3 refers to field studies
TableS3.Overview of studies investigating potential adverse effects of purified Cry3Bb1 protein or Cry3Bb1-containing food of vegetal origin on non-target soil arthropods
Order: Family / Functional group (feeding guild)* / Geographical region where higher-tier study was conducted / Stage tested / Reported effect / Type of study / Test material / Reference(see Devos et al. (2012) for reference list)
Species
Acari
Various species / Mixed (fungivore; algivore; detritivore; carnivore) / Kansas (USA) / Larva & adult / No effect of Bt-maize on abundance and populations, seed coat or soil insecticides / Tier 3 / MON863, MON853 / Al-Deeb et al. (2003)
Kansas (USA) / Larva & adult / No effect of Bt-maize, seed coat or soil insecticides on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
- / Larva & adult / No effect of Bt-maize on abundance in litter bags; no differences among conventional maize varieties / Tier 2 / MON88017 leaf material / Hönemann et al. (2008)
Acari: Acaridae
Rhizoglyphus robini / Herbivore / New York (USA) / Larva & adult / No effect of seed coat or soil insecticide on abundance; no effect of Bt-maize with seed coat / Tier 3 / MON863 / Carter et al. (2004)
Collembola
Various species / Decomposer (detritivore) / Kansas (USA) / Larva & adult / No effect of Bt-maize, seed coat or soil insecticides on abundance and populations / Tier 3 / MON863, MON853 / Al-Deeb et al. (2003)
Kansas (USA) / Larva & adult / No effect of Bt-maize, seed coat or soil insecticides on abundance / Tier 3 / MON863 / Ahmad et al. (2005)
Iowa and Illinois (USA) / Larva & adult / No effect of Bt-maize or seed coat on abundance; increased abundance after application of soil insecticides / Tier 3 / MON863 / Bitzer et al. (2005)
- / Larva & adult / No effect of Bt-maize on abundance in litter bags; no differences among conventional maize varieties / Tier 2 / MON88017 leaf material / Hönemann et al. (2008)
Collembola: Isotomidae
Folsomia candida / Decomposer (detritivore) / - / Larva & adult / No effect on survival and offspring production / Tier 1b / MON859 leaf material; 8.73, 87.3 and 872.5μg/g diet / Teixeira (1999)
* Functional group is the primary functional group for which a species is valued