Physiological and biochemical responses to cold and drought

in the rock-dwelling pulmonate snail, Chondrina avenacea.

Vladimír Koštál *, Jan Rozsypal, Pavel Pech, Helena Zahradníčková, Petr Šimek

* Corresponding author: Biology Centre of the Academy of Sciences

Institute of Entomology

Branišovská 31

370 05 České Budějovice

Czech Republic

E-mail:

Phone: +420 387775324

Fax: +420 385310354

Online Resource 3:

Detailed analysis of physiological parameters in Chondrina avenacea exposed to drought conditions. The snails were sampled in the field during September 2010 and were exposed in the laboratory to air of either low r.h. 10% or high r.h. ~60%, both at 25°C, for 6w. All values in the Table are shown as the mean ± S.D.

Parameter [units]
(n) / Field
Sep 2010 / Lab / 6w
25°C/r.h.~60% / t-test P
a / Lab / 6w
25°C/r.h.<10% / t-test P
a
TFM [mg] group 1
(20) / 14.24 ± 2.51 / 13.55 ± 2.32 / 0.3723 ns / x / x
TFM [mg] group 2
(20) / 13.75 ± 1.70 / x / x / 13.00 ± 1.69 / 0.1099 ns
TFM loss [mg] / x / 0.69 ± 0.41 / x / 0.76 ± 0.15 / 0.4777 ns
TFM loss [%] / x / 4.76 ± 2.35 / x / 5.58 ± 1.27 / 0.1779 ns
BWM loss [%] / x / 11.09 ± 5.48 / x / 13.01 ± 2.97 / 0.1757 ns
SCP [°C]
(16) / -9.0 ± 2.4 (16) / -11.1 ± 1.5 (16) / 0.0044 ** / -11.1 ± 1.2 (16) / 0.0057 **
Lt50-5°C [days] / 1.2 b / > 30 c / x / n.a. / x
Oxygen
[µl O2.g-1 FM.h-1]
(8 x 10) / 74.8 ± 13.8 / 31.0 ± 5.9 / 0.0072 ** / n.a. / x
Glycogen
[µg.mg-1 DM]
(4 x 5) / 91.5 ± 17.8 / 71.20 ± 11.05 / 0.0661 ns / 57.56 ± 22.79 / 0.0172 **
Lactate [mM]
(3 x 5) / 0.566 ± 0.118 / 1.948 ± 2.706 / 0.4268 ns / 0.891 ± 0.480 / 0.3183 ns
Alanine [mM]
(3 x 5) / 1.745 ± 0.436 / 2.729 ± 1.168 / 0.2434 ns / 1.625 ± 0.328 / 0.7226 ns
Succinate [mM]
(3 x 5) / 0.230 ± 0.013 / 0.646 ± 0.318 / 0.0863 ns / 0.600 ± 0.232 / 0.0510 ns

n.a., parameter was not analyzed;

x, empty cell;

a unpaired, two-tailed t-tests were used to compare differences between means of relevant groups

(ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001);

b these data are shown in detail in Fig. 7;

c Lt50-5 ºC was not reached in these snails; n = 4 groups of 20 snails each (survival was as

follows: exposure for 3d, survival 95%; 7d, 95%; 14d, 90%, and 30d, 85%).