Table S1 Primers for Mirnas Quantification, Expressing Vectors, Luciferase Reporter Plasmids

Table S1 Primers and probes for miRNAs quantification, expressing vectors, luciferase reporter plasmids.

miRNA / Primer / Sequence (5’-3’)
miR-17 / RT primer / GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACC
Forward primer / GAGCCAAAGTGCTTACAGTGC
Reverse primer / AGTGCAGGGTCCGAGGTATT
miR-20a / RT primer / GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACC
Forward primer / TGGGTAAAGTGCTTATAGTGC
Reverse primer / AGTGCAGGGTCCGAGGTATT
miR-26a / RT primer / GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCTA
Forward primer / CCGCCGTTCAAGTAATCCAG
Reverse primer / AGTGCAGGGTCCGAGGTATT
miR-26b / RT primer / GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCTAT
Forward primer / CGCCGCTTCAAGTAATTCAGGAT
Reverse primer / GTGCAGGGTCCGAGGT
let-7d / RT primer / GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACTAT
Forward primer / GCCGAGAGGTAGTAGGTTGCATAG
Reverse primer / AGTGCAGGGTCCGAGGTATT
miR-19b / RT primer / GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGT
Forward primer / GGGCAAATCCATGCAAAAC
Reverse primer / AGTGCAGGGTCCGAGGTATT
U6 / Forward primer / CGCTTCGGCAGCACATATAC
Reverse primer / CAGGGGCCATGCTAATCTT
STAT3 / Forward primer / CCGCTCGAGATGGCCCAATGGAATCAGCTAC
Reverse primer / ATCGTTAACTCACATGGGGGAGGTAGCGC
p21 / Forward primer / ATGTCAGAACCGGCTGGGGATG
Reverse primer / TTAGGGCTTCCTCTTGGAGAAGATC
VEGF / Reverse primer / GCAGAATCATCACGAAGTGG
Reverse primer / GCATGGTGATGTTGGACTCC
Glut-1 / Forward primer / GACTCCTGCCCTGTTGTG
Reverse primer / CGAAGTCTAAGCCGTTGC
-actin / Forward primer / CATCCTCACCCTGAAGTACCC
Reverse primer / AGCCTGGATAGCAACGTACATG
miR-17
(DNA constructs) / Forward primer / TGTTAGAGTTTGAGGTGTTAATTC
Reverse primer / CACTTAGGGCAGTAGATGCT
miR-20a
(DNA constructs) / Forward primer / TTTGTTTGCAGTCCTCTGTT
Reverse primer / GGGTTTGAGTTTCCCTTACTTTTCT
STAT3
(DNA constructs) / Forward primer / GCCCAATGGAATCAGCTACAG
Reverse primer / TTATTTCCAAACTGCATCAATGAATGGTG
p21
(DNA constructs) / Forward primer / ATGTCAGAACCGGCTGGGGA
Reverse primer / TTAGGGCTTCCTCTTGGAGAAGAT
c-Myc
(DNA constructs) / Forward primer / CTGGATTTTTTTCGGGTAGTGG
Reverse primer / TTACGCACAAGAGTTCCGTAG
STAT3-UTR / Forward primer / TGCAGAAAGATACGACTGAG
Reverse primer / TTATAGGTAGGTAAGCAACCC
p21-UTR / Forward primer / TGATCTTCTCCAAGAGGAAGCCCT
Reverse primer / ATTCAGCATTGTGGGAGGAGCTGT
STAT3-UTR-Mut-1 / Forward primer / CTGCTATCTTTGAGCAATCTGGGGAGTTTTAAAAATAGAGAAATGAGTGAA
Reverse primer / TTCACTCATTTCTCTATTTTTAAAACTCCCCAGATTGCTCAAAGATAGCAG
STAT3-UTR-Mut-2 / Forward primer / GCTACATACTCCTGGCATTGGAGTGTTTAACCTTGCTGACATCC
Reverse primer / GGATGTCAGCAAGGTTAAACACTCCAATGCCAGGAGTATGTAGC
p21-UTR-Mut-1 / Forward primer / TTTCATTTGAGAAGTAAACAGATGGGAGTGTGAAGGGGCCTCACCGAGTGGGGGCATCATC
Reverse primer / GATGATGCCCCCACTCGGTGAGGCCCCTTCACACTCCCATCTGTTTACTTCTCAAATGAAA
p21-UTR-Mut-2 / Forward primer / CCCCATCCCTCCCCAGTTCATTGGAGTGTGATTAGCAGCGGAACAAGGAG
Reverse primer / CTCCTTGTTCCGCTGCTAATCACACTCCAATGAACTGGGGAGGGATGGGG
miR-17 probe / Enhanced by LNA / CTACCTGCACTGTAAGCACTTTG
miR-20a probe / Enhanced by LNA / CTACCTGCACTATAAGCACTTTA
U6 probe / Enhanced by LNA / CACGAATTTGCGTGTCATCCTT
shR-Luc / sequence / CGTACGCGGAATACTTCGA
shR-p21-1 / sequence / GACCATGTGGACCTGTCAC
shR-p21-2 / sequence / AATGGCGGGCTGCATCCAGGA
shR-ST-1 / sequence / CTTCAGACCCGTCAACAAA
shR-ST-2 / sequence / CATCTGCCTAGATCGGCTA
shR-α14 / sequence / GACAGTACAGGATGCTTGC
shR-α16 / sequence / GTGGATTACCACAGCTGAC
shR-β2 / sequence / CATTGAGGGTATCTTCACT
shR-β5 / sequence / GAGACAGCTTCCAACAGGT
shR-C2 / sequence / CCTTG TGCCTTGGAAATGC