SUPPORTING TABLEEstimates of mitochondrial genetic diversity and mitotype (mtDNA haplotype) frequencies in allopatric populations of black spruce and red spruce and populations from the zone of contact between the two species.

Mitotype frequencya
Species – Population typeb / I / II / III / IV / V / VI / VII / VIII / IX / X
(Latitude / Longitude) / n / H / nh / (B2FCA) / (A2FCA) / (B2EBB) / (A2EBB) / (B2FCB) / (A2EBA) / (B2EBA) / (B2GDA) / (A2FCB) / (A2ECC)
Black spruce allopatric populations
1-Thunder Bay, ON
48° 25 N / 89° 25 W / 20 / 0 / 1 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
2- Radisson, QC
53° 34 N / 76° 10 W / 20 / 0 / 1 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
3- Rivière Grande-Baleine,QC
55° 09 N / 75° 29 W / 20 / 0 / 1 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
4- Lac Chavigny, QC
58° 11 N / 75° 21 W / 28 / 0 / 1 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
5- Pinus River, Lab
61° 41 N / 58° 48 W / 20 / 0.180 / 2 / 0.100 / 0 / 0 / 0 / 0 / 0 / 0 / 0.900 / 0 / 0
6- Groves Point, Lab
67° 30 N / 59° 17 W / 20 / 0.320 / 2 / 0 / 0 / 0 / 0.200 / 0 / 0 / 0 / 0.800 / 0 / 0
7- Sheffield Lake Rd., NF
49° 17 N / 56° 27 W / 20 / 0 / 1 / 0 / 0 / 0 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0
8- Sheffield Lake, NF
49° 21 N / 56° 39 W / 20 / 0 / 1 / 0 / 0 / 0 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0
9- Lac Chibougamau, QC
49° 12 N / 73° 36 W / 30 / 0.722 / 7 / 0.467 / 0.033 / 0.067 / 0.200 / 0.100 / 0.100 / 0.033 / 0 / 0 / 0
10- Parc Mistassini, QC
50° 27 N / 73° 38 W / 15 / 0.632 / 5 / 0.533 / 0.067 / 0.067 / 0.266 / 0 / 0.067 / 0 / 0 / 0 / 0
11- Manicouagan, QC
50° 40 N / 68° 46 W / 15 / 0.587 / 5 / 0.067 / 0.200 / 0.066 / 0.600 / 0 / 0 / 0.067 / 0 / 0 / 0
12- Saguenay, QC
48° 24 N / 69° 30 W / 30 / 0.488 / 3 / 0.100 / 0 / 0.230 / 0.670 / 0 / 0 / 0 / 0 / 0 / 0
13- De Beaujeu, QC
49° 03 N / 65° 00 W / 30 / 0.604 / 7 / 0.033 / 0.067 / 0.134 / 0.600 / 0.033 / 0 / 0 / 0.100 / 0 / 0.033
14- Ligneris, QC
48° 55 N / 78° 30 W / 30 / 0 / 1 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
15- St-Michel des Saints, QC
46° 50 N / 74° 25 W / 10 / 0.340 / 3 / 0.800 / 0.100 / 0 / 0.100 / 0 / 0 / 0 / 0 / 0 / 0
16- Cramolet, QC
49° 15 N / 77° 25 W / 12 / 0.722 / 5 / 0.417 / 0.250 / 0.083 / 0.167 / 0.083 / 0 / 0 / 0 / 0 / 0
Mean / ― / 0.353 / 3.31 / 0.424 / 0.055 / 0.050 / 0.293 / 0.016 / 0.013 / 0.008 / 0.139 / 0 / 0.003

SUPPORTING TABLE Continued.

Mitotype frequencya
Species – Population type b / I / II / III / IV / V / VI / VII / VIII / IX / X
(Latitude / Longitude) / n / H / nh / (B2FCA) / (A2FCA) / (B2EBB) / (A2EBB) / (B2FCB) / (A2EBA) / (B2EBA) / (B2GDA) / (A2FCB) / (A2ECC)
Populations from the zone of contact dominated by black spruce
17- Parc des Grands Jardins, QC
47° 48 N / 70° 54 W / 30 / 0.686 / 6 / 0.134 / 0.267 / 0.067 / 0.467 / 0.033 / 0 / 0 / 0 / 0.033 / 0
18- Tourville, QC
47° 06 N / 70° 00 W / 50 / 0.269 / 2 / 0 / 0 / 0.160 / 0.840 / 0 / 0 / 0 / 0 / 0 / 0
19- Villeroy, QC
46° 12 N / 71° 30 W / 50 / 0.422 / 3 / 0 / 0 / 0 / 0.720 / 0.040 / 0 / 0 / 0 / 0.240 / 0
20- Black Lake, QC
45° 36 N / 71° 12 W / 15 / 0.659 / 6 / 0 / 0 / 0.200 / 0.532 / 0.067 / 0.067 / 0.067 / 0 / 0.067 / 0
21- Laurier Station, QC
46° 18 N / 71° 24 W / 15 / 0.667 / 5 / 0.067 / 0 / 0.400 / 0.400 / 0.067 / 0 / 0 / 0 / 0.067 / 0
22- Condé, QC
49° 06 N / 72° 44 W / 30 / 0.762 / 7 / 0.233 / 0.134 / 0.033 / 0.367 / 0.033 / 0 / 0.167 / 0.033 / 0 / 0
23- Parc de la Vérendrye, QC
47° 05 N / 74° 33 W / 12 / 0 / 1 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Mean / ― / 0.495 / 4.29 / 0.205 / 0.057 / 0.123 / 0.475 / 0.034 / 0.010 / 0.033 / 0.005 / 0.058 / 0
Mixed populations from the zone of contact
24- La Patrie, QC
45° 12 N / 71° 06 W / 15 / 0.125 / 2 / 0 / 0 / 0.067 / 0.933 / 0 / 0 / 0 / 0 / 0 / 0
25- St-Gabriel-de-Valcartier, QC
46° 30 N / 71° 18 W / 15 / 0.436 / 4 / 0.067 / 0 / 0.133 / 0.733 / 0 / 0 / 0 / 0.067 / 0 / 0
26- St-Raymond, QC
46° 30 N / 71° 30 W / 15 / 0.125 / 2 / 0 / 0 / 0.067 / 0.933 / 0 / 0 / 0 / 0 / 0 / 0
27- West Ditton, QC
45° 12 N / 71° 12 W / 15 / 0.426 / 3 / 0 / 0 / 0.133 / 0.734 / 0 / 0 / 0 / 0 / 0.133 / 0
28- St-Méthode Frontenac, QC
46° 00 / 71° 00 W / 15 / 0 / 1 / 0 / 0 / 0 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0
29- St-Louis de Blanford, QC
46° 10 N / 72° 00 W / 15 / 0.773 / 7 / 0.400 / 0.067 / 0.067 / 0.133 / 0.133 / 0.133 / 0.067 / 0 / 0 / 0
Mean / ― / 0.314 / 3.17 / 0.077 / 0.011 / 0.077 / 0.744 / 0.022 / 0.022 / 0.011 / 0.011 / 0.022 / 0

SUPPORTING TABLE Completed.

Mitotype frequencya
Species – Population type b / I / II / III / IV / V / VI / VII / VIII / IX / X
(Latitude / Longitude) / n / H / nh / (B2FCA) / (A2FCA) / (B2EBB) / (A2EBB) / (B2FCB) / (A2EBA) / (B2EBA) / (B2GDA) / (A2FCB) / (A2ECC)
Populations from the zone of contact dominated by red spruce
30- Estrie, QC
45° 12 N / 71° 06 W / 50 / 0.649 / 4 / 0 / 0 / 0.340 / 0.460 / 0.060 / 0 / 0.140 / 0 / 0 / 0
31- Ste-Anne-de-Beaupré, QC
47° 00 N / 70° 36 W / 15 / 0.515 / 4 / 0 / 0.133 / 0.133 / 0.667 / 0 / 0 / 0 / 0 / 0.067 / 0
32- Duchesnay, QC
46° 52 N / 71° 39 W / 35 / 0.385 / 2 / 0 / 0 / 0.257 / 0.743 / 0 / 0 / 0 / 0 / 0 / 0
33- Fredericton, NB
46° 00 N / 66° 24 W / 50 / 0.241 / 2 / 0 / 0 / 0.140 / 0.860 / 0 / 0 / 0 / 0 / 0 / 0
34- Lac Trois-Saumons, QC
47° 06 N / 70° 06 W / 15 / 0.508 / 4 / 0 / 0 / 0.200 / 0.666 / 0 / 0.067 / 0 / 0 / 0.067 / 0
35- Lauzon, QC
46° 24 N / 71° 06 W / 15 / 0 / 1 / 0 / 0 / 0 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0
36- St-Sébastien, QC
45° 30 N / 70° 30 W / 15 / 0.418 / 2 / 0 / 0 / 0.200 / 0.733 / 0 / 0 / 0 / 0 / 0.067 / 0
Mean / ― / 0.388 / 2.71 / 0 / 0.019 / 0.181 / 0.732 / 0.008 / 0.009 / 0.020 / 0 / 0.029 / 0
Red spruce allopatric populations
37- October M.S.F., MA
42° 24 N / 73° 12 W / 15 / 0.125 / 2 / 0 / 0 / 0.067 / 0.933 / 0 / 0 / 0 / 0 / 0 / 0
38- Upper Jay, NY
44 ° 24 N / 73° 42 W / 15 / 0.231 / 2 / 0 / 0 / 0.133 / 0.867 / 0 / 0 / 0 / 0 / 0 / 0
39- Bear Meadows, PA
40° 42 N / 77° 42 W / 15 / 0 / 1 / 0 / 0 / 0 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0
40- Glade Run, WV
38 ° 36 N / 79 ° 48 W / 15 / 0 / 1 / 0 / 0 / 0 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0
41- Indian Cap, NC
35° 36 N / 83° 30 W / 15 / 0 / 1 / 0 / 0 / 0 / 1.0 / 0 / 0 / 0 / 0 / 0 / 0
Mean / ― / 0.071 / 1.4 / 0 / 0 / 0.040 / 0.960 / 0 / 0 / 0 / 0 / 0 / 0

Abbreviations: n = number of sampled trees, H = mtDNA genetic diversity, nh = observed number of mitotypes, ON = Ontario, QC = Québec, Lab = Labrador, NF = Newfoundland; NB = New Brunswick, MA = Massachusetts, NY = New York, PA = Pennsylvania, WV = West Virginia, NC =North Carolina. a Multi-locus genotypes (SSU rRNA V1 region, nad1 intron b/c, nad5 intron 1 and nad7 intron 1, respectively) defining each mitotype are indicated in parentheses. b Populations were grouped according to their location relative to the zone of contact between black spruce and red spruce and to the relative abundance of each species in the sampled stands: 1. Black spruce allopatric populations: black spruce stands located north and west from the known zone of contact (between 250 and 2,200 km) and consisting of putative black spruce individuals only; 2. Populations from the zone of contact dominated by black spruce: stands in the zone of contact with prevalent occurrence of black spruce in the sexually mature population (70 to 100% relative abundance); 3. Mixed populations from the zone of contact: stands where each species was about equally represented; 4. Populations from the zone of contact dominated by red spruce: stands in the zone of contact with prevalent occurrence of red spruce in the sexually mature population (70 to 100% relative abundance); 5. Red spruce allopatric populations: stands located south of the zone of contact (between 150 and 1,300 km) and consisting of putative red spruce individuals only.