Result: ( ) Approved for BSL-2 Work ( ) Not Approved for BSL-2 Work

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Inspection Checklist for BSL-2 Laboratories (BMBL 5th edition; NIH Guidelines)
Lab PI/Contact Person: / Inspection Date: / Inspected By:
Lab Location (Bldg/Rm #) / Dept: / Phone #:
Inspection Type: ( ) Initial ( ) Annual
List of Agents In Use
( ) Recombinant DNA: ( ) Bacteria ( ) Parasite: ( ) Toxin: ( ) Virus: ( ) Prion: ( ) Fungus: ( ) Human: ( ) Select Agent: ( ) Other:
Reference / Statement / Response / Comments
Yes / No / NA
A Standard Microbiological Practices
BMBL: A1
NIH: G-II-B-1-a
NIH: G-II-B-2-b / The PI must enforce the institutional policies that control access to the laboratory. Access is limited/restricted by the PI when work is in progress.
BMBL: A2
NIH: G-II-B-1-f / Persons must wash their hands after working with potentially hazardous materials and before leaving the laboratory
BMBL: A3
NIH: G-II-B-1-e / Eating, drinking, smoking, handling contact lenses, applying cosmetics and storing food for human consumption must not be permitted in laboratory areas.
BMBL: A3 / Food must be stored outside the laboratory area in cabinets or refrigerators designated and used for this purpose
BMBL: A4
NIH: G-II-B-1-d / Mouth pipetting is prohibited; mechanical pipetting devices must be used
BMBL: A5 / Policies for safe handling of sharps must be developed and implemented.
BMBL: A5
NIH: G-II-B-2-j / Whenever practical, laboratory supervisors should adopt improved engineering/work practice controls that reduce risk of sharps injury.
BMBL: A5 / Precautions, listed below, must always be taken with sharps items.
Reference / Statement / Response / Comments
Yes / No / NA
BMBL: A5-a / Needles must not be bent, sheared, broken, recapped, removed from disposable syringes or otherwise manipulated by hand before disposal.
BMBL: A5-b / Place used disposable needles/syringes in puncture-resistant containers.
BMBL: A5-c / Non-disposable needles are placed in a hard walled container for transport to decontamination area (autoclave).
BMBL: A5-d / Broken glassware must be handled with a mechanical device (tongs, forceps, dustpan/brush) not with hands.
BMBL: A5-d / Substitute plastic ware whenever possible.
BMBL: A6
NIH: G-II-B-1-g / Perform all procedures to minimize the creation of splashes/aerosols.
BMBL: A7
NIH: G-II-B-1-b / Decontaminate work surfaces after completion of work and after any spill or splash of biohazardous material. Use the appropriate disinfectant.
BMBL: A8
NIH: G-II-B-1-c / Decontaminate all cultures, stocks, and other biohazardous material before disposal.
BMBL: A8 / Depending on where the decontamination will be performed, use the following methods prior to transport.
BMBL: A8-a / Materials to be decontaminated outside of the immediate lab must be placed in a durable, leak-proof container and secured for transport.
BMBL: A8-b / Materials to be removed from the facility for decontamination must be packed in accordance with applicable regulations.
BMBL: A9
NIH: G-II-B-2-d / A sign incorporating the universal biohazard symbol must be posted at the entrance to the lab when biohazardous materials are present. Posted information must include: the biosafety level, the responsible person’s name, telephone number, and entry/exit procedures.
Reference / Statement / Response / Comments
Yes / No / NA
BMBL: A9 / Agent information should be posted in accordance with institutional policy.
BMBL: A10
NIH: G-II-B-2-e / An effective integrated pest management program is required. See appendix G.
BMBL: A11 / The lab supervisor must ensure that lab personnel receive appropriate training regarding their duties, precautions to prevent exposures, and exposure evaluation procedures. Personnel must receive annual updates or additional training when procedures or policy changes occur.
BMBL: A11 / Personal health status may impact an individual’s susceptibility to infection, ability to receive immunizations or prophylactic interventions. Therefore, all laboratory personnel and particularly women of child-bearing age should be provided with information regarding immune competence and conditions that may predispose them to infection. Individuals having these conditions should be encouraged to self-identify to the institution’s healthcare provider for appropriate counseling and guidance.
NIH: G-II-B-1-h / Experiments of lesser biohazard potential can be conducted concurrently in carefully demarcated areas of the same lab.
BMBL: B Special Microbiological Practices
BMBL: B1
NIH: G-II-B-2-c / All persons entering the lab must be advised of the potential hazards and meet specific entry requirements.
BMBL: B2 / Lab personnel must be provided medical surveillance and offered appropriate immunizations for agents in the lab.
BMBL: B3
NIH: G-II-B-2-l / When appropriate, a baseline serum sample should be stored.
BMBL: B4
NIH: G-II-B-2-m / A lab-specific biosafety manual must be prepared and adopted as policy.
Reference / Statement / Response / Comments
Yes / No / NA
BMBL: B4 / The manual must be available and accessible.
BMBL: B5 / The lab supervisor must ensure that lab personnel demonstrate proficiency in standard and special microbiological practices before working with BSL-2 agents.
BMBL: B6
NIH: G-II-B-2-a / Potentially biohazardous material must be placed in a durable, leak-proof container during collection, handling, processing, storage, or transport within a facility.
BMBL: B7 / Lab equipment should be routinely decontaminated, as well as, after spills, splashes, or other potential contamination.
BMBL: B7-a / Spills involving biohazardous material must be contained, decontaminated, and cleaned up by staff properly trained and equipped to work with biohazardous material.
BMBL: B7-b / Equipment must be decontaminated before repair, maintenance or removal from the lab.
BMBL: B8
NIH: G-II-B-2-k / Incidents that may result in exposure to biohazardous material must be immediately evaluated and treated according to procedures described in the lab biosafety manual. All such incidents must be reported to the lab supervisor.
BMBL: B9
NIH: G-II-B-2-g / Animals and plants not associated with the work must not be permitted in the lab.
BMBL: B10 / All procedures involving the manipulation of biohazardous material that may generate an aerosol should be conducted in a BSC or other physical containment device.
Reference / Statement / Response / Comments
Yes / No / NA
BMBL: C Safety Equipment (Primary Barriers and PPE)
BMBL: C1-a
NIH: G-II-B-3-a
NIH: G-II-B-3-a-(1) / Properly maintained BSCs (preferably Class II), other appropriate PPE, or other physical containment devices must be used whenever:
Procedures with potential for creating infectious aerosols or splashes are conducted (examples: pipetting, centrifuging, grinding, blending, shaking, mixing, sonicating, opening containers of material, intranasal animal inoculation, harvesting infected tissues from animals or eggs).
BMBL: C1-b
NIH: G-II-B-3-a-(2) / Properly maintained BSCs (preferably Class II), other appropriate PPE, or other physical containment devices must be used whenever:
High concentrations or large volumes of material are used.
Such materials may be centrifuged in the open lab using sealed rotor heads or centrifuge safety cups.
BMBL: C2
NIH: G-II-B-2-f / Protective lab coats, gowns, smocks or uniforms designated for lab use must be worm while working with hazardous material.
BMBL: C2
NIH: G-II-B-2-f / Remove PPE before leaving non-lab areas (e.g. cafeteria, library admin offices).
BMBL: C2 / Dispose of PPE appropriately or deposit it for laundering by the institution. It is recommended that lab clothing not be taken home.
BMBL: C3 / Eye and face protection (goggles, mask, face shield or other splatter guard) is used for anticipated splashes or sprays of material when handled outside of the BSC.
BMBL: C3 / Eye and face protection must be disposed of with other contaminated lab waste or decontaminated before reuse.
BMBL: C3 / Persons who wear contact lenses in labs should also wear eye protection.
Reference / Statement / Response / Comments
Yes / No / NA
BMBL: C4
NIH: G-II-B-2-h / Gloves must be worn to protect hands from exposure to hazardous material.
BMBL: C4 / Glove selection should be based on an appropriate risk assessment.
BMBL: C4 / Alternatives to latex gloves should be available.
BMBL: C4 / Gloves must not be worn outside the lab.
BMBL: C4-a / In addition, BSL-2 workers should:
Change gloves when contaminated, integrity has been compromised or when otherwise necessary.
BMBL: C4-a / In addition, BSL-2 workers should:
Wear two pairs of gloves when appropriate.
BMBL: C4-b / In addition, BSL-2 workers should:
Remove gloves and wash hands when work with the material has been completed and before leaving the lab.
BMBL: C4-c / In addition, BSL-2 workers should:
Not wash or reuse disposable gloves.
BMBL: C4-c / In addition, BSL-2 workers should:
Dispose of used gloves with other contaminated lab waste.
BMBL: C4-c / Hand washing protocols must be rigorously followed.
BMBL: C5 / Eye, face and respiratory protection should be used in rooms containing infected animals as determined by the risk assessment.
Reference / Statement / Response / Comments
Yes / No / NA
BMBL: D Laboratory Facilities (Secondary Barriers)
BMBL: D1 / Lab doors should be self-closing and have locks in accordance with institutional policies.
BMBL: D2
NIH: G-II-B-4-d / Labs must have a sink for hand washing. The sink may be manual, hands-free or automatic. It should be located near the exit door.
BMBL: D3
NIH: G-II-B-4-a / The lab should be designed so that it can be easily cleaned and decontaminated.
BMBL: D3 / Carpets and rugs are not permitted.
BMBL: D4
NIH: G-II-B-4-c / Lab furniture must be capable of supporting anticipated loads and uses.
BMBL: D4
NIH: G-II-B-4-c / Spaces between benches, cabinets and equipment should be accessible for cleaning.
BMBL: D4-A
NIH: G-II-B-4-b / Bench tops must be impervious to water, resistant to heat, organic solvents, acids, alkali and other chemicals.
BMBL: D4-b / Chairs used in lab work must be covered with a non-porous material that can be easily cleaned and decontaminated.
BMBL: D5
NIH: G-II-B-4-e / Lab windows to the exterior are not recommended. However, if a lab does have windows that open to the exterior, they must be fitted with fly screens.
BMBL: D6 / BSCs must be installed so that fluctuations of the room air supply and exhaust do not interfere with proper operations.
BMBL: D6 / BSCs should be located away from doors, windows that can be opened, heavily traveled lab areas, and other possible lab flow disruptions.
Reference / Statement / Response / Comments
Yes / No / NA
BMBL: D7 / Vacuum lines should be protected with HEPA filters or their equivalent. Filters must be replaced as needed. Liquid disinfectant traps may be required.
BMBL: D8 / An eyewash station must be readily available.
BMBL: D9 / There are no specific requirements on ventilation systems. However, planning of new facilities should consider mechanical ventilation systems that provide an inward flow of air without recirculation to spaces outside of the lab.
BMBL: D10 / HEPA filtered air form a Class II BSC can be safely recirculated back into the lab if the cabinet is tested and certified at least annually and operated according to manufacturer’s recommendations. BSCs can also be connected to the lab exhaust system by either a thimble canopy or by a hard connection.
BMBL: D10 / Provisions to assure proper BSC performance and air system operation must be verified.
BMBL: D11
NIH: G-II-B-2-i
NIH: G-II-B-4- / A method for decontaminating all lab wastes should be available in the facility (e.g. autoclave, chemical disinfection, incineration or other validated decontamination method).
Mississippi State Department of Health “Adopted Standards for Regulation of Medical Waste-1989” / Autoclave Verification Program: Any autoclave used to sterilize infectious medical waste should perform both load and monthly checks using a chemical indicator and a biological indicator respectively.

Result: ( ) Approved for BSL-2 work ( ) Not approved for BSL-2 work

Notes:

BSO Signature:______Date:______

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