Article Title: DNA Methylation Status Is Associated with the Formation of Heterosis In

Article Title:DNA methylation status is associated with the formation of heterosis in Larix kaempferi intraspecific hybrids

Journal Name: Molecular Breeding

Authors: Ai Li1, 2·Wen-Qin Song1·Cheng-Bin Chen1 · Ya-Nan Zhou1· Li-Wang Qi3·Chun-Guo Wang*1

Affiliation:1College of life sciences, Nankai University, Tianjin 300071, China;2Department of Horticulture, Tianjin Agricultural University, Tianjin, 300384, China; 3Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

*E-mail of corresponding author:

Supplementary Table8: Summary of MSAP polymorphic bands analyzed by qRT-PCR

MSAP Fragments / DNA methylation pattern / qRT-PCR primers / correlation between DNA methylation and gene expression
P1 / P2 / F1 / F1’
MF1 / ○ / ○ / ● / F: 5’-TATGTTGTTGGCTACTTTTATTTCA-3’
R: 5’-TATTAGTCGGTTCCTCCCCTGT-3’ / Y
MF3 / ○ / ○ / ● / F: 5’-CCCTGCTCAACTGGGAAACC-3’
R: 5’-CGGCACTGGGCATGAAAGA-3’ / Y
MF4 / ● / ● / ○ / F: 5’-CACACTTGAGGAAGGGACAAAACA-3’
R: 5’-ACACCACATCACATTCGATCTACAC-3’ / N
MF5 / ○ / ○ / ● / F: 5’-TTCTCAGGTGCCTTAGGTGCG-3’
R: 5’-TATGGTGTTCCCAGAGTTTTGTTC-3’ / Y
MF9 / ● / ● / ○ / F: 5’-TTAAGACGGAATCTCAATTATTGG-3’
R: 5’-TTCCCAACGAACAATGACTAATC-3’ / N
MF11 / ○ / ○ / ● / F: 5’-TCGCCGAATCAATCACATACAA-3’
R: 5’-TACGAAAACGAGCACTTAGAACAC-3’ / Y
MF12 / ● / ● / ○ / F: 5’-ACAAGTATTGGGTCAAGTTTCTGGT-3’
R: 5’-GCGGTAGGGTTTTCGATCTG-3’ / Y
MF13 / ● / ● / ● / F: 5’-CGTGGAATGGGATTAGAAAAGGAA-3’
R: 5’-GCAAAGGACCAACAACTATTTAGCA-3’ / N
MF14 / ● / ● / ○ / F: 5’-AATCTTCGGGTGTTCGTTGGT-3’
R: 5’-AGAGCCTTCAGATACAAGCAGCA-3’ / N
MF16 / ○ / ○ / ● / F: 5’-ATGGCGGTGTCTCACAAATG-3’
R: 5’-GCCCAGAAATCAATGGAACTTA-3’ / Y
MF19 / ● / ● / ○ / F: 5’-TGTATGCAAACTGTCGGTTTATAGA-3’
R: 5’-TCAACAAGCCCATTCTGTCCAT-3’ / Y
MF20 / ○ / ○ / ● / F: 5’-AAGCCGACTCCCGTTTATCA-3’
R: 5’-GCATTGGTTAGGTCCTTTTCAG-3’ / Y

P1: Larix13, P2: Larix82, F1:Larix82×Larix13-6, F1’: Larix13×Larix82-21, “●”methylation, “○”unmethylation, “Y” showed a negative correlation between DNA methylation and gene expression, “N” showed no negative correlation between DNA methylation and gene expression