Supporting Infromation

SUPPORTING INFROMATION

Near-infrared light sensitive liposomes for the enhanced photothermal tumor treatment by the combination with chemotherapy

Jian You1*, Peizun Zhang1, Fuqiang Hu1*, Yongzhong Du1, Hong Yuan1, Jiang Zhu2*, Zuhua Wang1, Jialin Zhou1, Chun Li2,

Authors’ Affiliations:

1College of Pharmaceutical Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, People’s Republic of China.

2Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, East Qingchun Road 3, Hangzhou 310016, People’s Republic of China.

3Department of Experimental Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.

*Corresponding Author:

Jian You, College of Pharmaceutical Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, People’s Republic of China; Tel: 086-571-88208443; Fax: 086-571-88208439; E-mail: .

Fuqiang Hu, College of Pharmaceutical Sciences, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, People’s Republic of China; E-mail: .

Jiang Zhu, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, East Qingchun Road 3, Hangzhou 310016, People’s Republic of China; E-mail: .

Table of Contents

fig. S1. IR spectrograms of OMP, OMP-HAuNS and HAuNS.

fig. S2. The zeta potential of HAuNS and OMP-HAuNS.

fig. S3. The size distributions of HAuNS and OMP-HAuNS.

fig. S4. The size distributions of DOX/HAuNS-TSL and blank TSL.

fig. S5. the temperature changes in DOX&HAuNS-TSL solutions under the irradiation by NIR laser.

fig. S6. The measurements of differential scanning calorimetry (DSC)

fig. S7. The release of DOX from DOX&HAuNS-NTSL with or without NIR laser irradiation

Table S1. physical properties of the liposomes

fig. S1 IR spectrograms of Octadecyl 3-Mercaptopionate (OMP), hollow gold nanospheres bonded Octadecyl 3-Mercaptopionate (OMP-HAuNS) and hollow gold nanospheres (HAuNS). The weak characteristic peak of mercapto group at the range of 2500cm-1~2900cm-1 (red circle) was disappeared completely, comparing with OMP alone, while other characteristic peaks for OMP still existed.

fig. S2. The zeta potential of HAuNS (a) and OMP-HAuNS (b).

fig.S3 The size distributions of HAuNS (a) and OMP-HAuNS (b).

fig.S4. The size distributions of DOX/HAuNS-TSL (a) and blank TSL (b).

fig. S5. Comparison of the temperature changes in DOX&HAuNS-TSL solutions and phosphate buffered saline (PBS) after irradiated by NIR laser.

fig. S6. The measurements of differential scanning calorimetry (DSC) of blank NTSL (broken line) and DOX&HAuNS-NTSL (real line). No phase transition temperature (Tm) was found for both blank liposomes and DOX&HAuNS-NTSL.

fig. S7. NIR-light-triggered release of DOX from DOX&HAuNS-NTSL with NIR laser irradiation over a period of 5 min at the output power of 3W (solid diamond) or without NIR laser irradiation.

Table S1 physical properties of the liposomes, Data represent the mean±standard deviation (n=3).

Liposomes / Diameter (nm) / Polydispersity / Zeta potential (mV) / EE (%)
DOX-TSL / 102.7±1.87 / 0.137±0.026 / -16.29±1.78 / 90.79
DOX&HAuNS-TSL / 154.8±1.76 / 0.186±0.029 / -38.05±1.7 / 91.46
DOX&HAuNS-NTSL / 161.8±2.12 / 0.205±0.024 / -49.79±3.91 / 96.53