For Sequencing, Fragments Were Amplified by Reverse Transcriptase Polymerase Chain Reaction

Supplement 1

For sequencing, fragments were amplified by reverse transcriptase polymerase chain reaction (RT-PCR) from total RNA extracts [3] of virus infected FgV-ch9 and non-infected wild-type strain PH1 mycelium cultivated in liquid complete medium[2]. The amplicons were blunt-end cloned into the pBlueSkriptSK-(Stratagene) with standard techniques[4]. cDNA was synthesized with primers RNA2 rev (5´- gccattaaagtcccatgac-3´), RNA 3 rev (5´- gccatttaagggttctccttaa-3´) and RNA 5 rev (5´- gccatttaggggtaggcc-3´), respectively, using RevertAid Reverse Transcriptase (Thermo Fisher Scientific, St. Leon-Rot, Germany) according to the manufacturers´ protocol. Two µl of the cDNA were amplified with 5 µl 5X Green GoTaq Reaction Buffer (Promega), 2 µl dNTPs (2 mM), 2 µl MgCl2 (25 mM), 1 µl (10 µM) of forward and reverse primer, respectively, and 1 U GoTaq DNA polymerase in a 25 µl volume in a Biometra T1 thermocycler (Biometra GmbH, Göttingen, Germany) with 4 min initial denaturation at 94 °C, 30 cycles with 30 sec 94 °C, 60 sec 58 °C and 60 sec at 72 °C. The reaction was completedafter 5 min at 72 °C. In detail, the 3´ terminus of segment 2 was amplified with the primers RNA 2 rev and RNA 2 fw 1 (5´- catattgtcacgagcgacg-3´). The duplication was verified by using primers RNA 2 rev and RNA 2 fw/rev (5´- agtcatgggactttaatggc-3´). The 3´ terminus of segment 3 was amplified with the primers RNA 3 rev 1 and RNA 3 coding fw 1 (5´- gcacctgtggatgatgactc-3´). The duplication was verified with the primer pair RNA 3 rev/RNA 3 fw/rev (5´- ttaaggagaacccttaaatggc-3´). The result of the verification of the duplication is given in Fig. S1A.

Absence of segments without duplications was verified by amplification of cDNA synthesized with primer RNA 2 fw 1 and RNA 3 coding fw 1The phenol-chloroform purified cDNA was C-tailed with Terminal Deoxynucleotidyl Transferase (Thermo Fisher Scientific) and amplified with primer polyG (5´-cggaattcccg15-3´) and RNA 2 fw 2 (5´-gttggtgattggacgggg-3´) for segment 2 and RNA 3 coding fw 2 (5´-ggtagaagcggcacgggcccgg-3´) for segment 3. For both segments, a single band was obtained (Fig. S1B).

The 3´ terminus of segment 5 was amplified with primers RNA 5 rev (5´- gccatttaggggtaggcc-3´) and RNA 5 fw (5´- gatttgcttcgagccacaacg-3´). The ORF of segment 5 was amplified with RNA 5 ORF rev (5´-tgttgtgtccggcagtatgt-3´) and RNA 5 ORF fw (5´-acataccagcaattccccgggatgtattcg-3´).

The fragment of ORF 5 in the pBlueSkriptSK-was subcloned SmaI/Hind III into the EcoRV/HindIII digested expression vector pET30a (Novagen, USA). The protein was expressed in BL21-Gold cells (Agilent Technologies, Santa Clara) according to the manufacturers´ instructions. A peptide with the amino acid sequence H-CQW SDE RIP TGP GYD L-Nh2 (amino acid 65 to 74 with an additional C for coupling to KLH) was synthesized in vitro at Eurogentec S.A. (Seraing, Belgium), coupled to keyhole limpet haemocyanin (KLH) carrier and used to produce a specific polyclonal antiserum against the ORF of segment 5 using the Speedy mini program (Eurogentec S.A., Seraing, Belgium). SDS-PAGE and Western-Blotting from total protein extracts of mycelium grown 3 days in liquid CM-medium was performed as described in Schwach et al. [5]. Bound antibodies were detected using alkaline phosphatase-conjugated secondary antibodies and nitro-blue tetrazolium chloride(NBT)/ 5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt (BCIP).

Sequencing and sequence analysis were performed as described [1]. Additional sequence alignments were done manually.Zinc finger motifs were predicted with prosite (

Fig. S1C: Verification of the absence of sequences without duplication.cDNA of the 3´ termini of segment 2 and 3, respectively, were C-tailed before they were amplified with a pair of polyG-primer and segment specific primer RNA2 fw 2 and RNA 3 coding fw 3, respectively. Amplification of the 3´ terminus of segment 2 (lane 1) and segment 3(lane 2) showed single bands. Amplicons were separated in 1,5 % agarose in TAE buffer and stained with ethidium bromide. M= GeneRuler™ 50 bp ladder (ThermoFisher Scientific).

[1] Darissa O, Willingmann P, Schäfer W, Adam G (2011) A novel double-stranded RNA mycovirus from Fusarium graminearum: nucleic acid sequence and genomic structure. Arch Virol 156: 647-658. doi:10.1007/s00705-010-0904-9

[2] Leach J, Lang BR, Yoder, OC (1982) Methods for selection of mutants and in vitro culture of Cochliobolusheterostrophus. J Gen Microbiol 128:1719–1729. doi:10.1099/00221287-128-8-1719

[3]Rott ME, Jelkmann W (2001) Characterization and detection of several filamentous viruses of cherry: adaption of an alternative cloning method (DOP-PCR) and modification of an RNA extraction protocol. Eur J Plant Pathol 107: 411-420. 10.1023/A:1011264400482

[4] Sambrook J, Russell DW, Janssen K, Argentine J (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A.

[5] Schwach F, Adam G, Heinze C (2004) Expression of a modified nucleocapsid-protein of Tomato spotted wilt virus (TSWV) confers resistance against TSWV and Groundnut ringspot virus (GRSV) by blocking systemic spread. Mol Plant Pathol 5: 309-316. doi: 10.1111/j.1364-3703.2004.00229.x

Supplement 2A

FgVCh9 segment 2

1 GCAAAAAAGA GAATAAAGCC ATTTGTGCAC ATAGGTGCGC GGGGAGAAAC TTTCGAACCG

61 TGGCAAATTT GCACCCTTGT TCTAACATAC ATCATGTCAG GACAATTGAA CATTCGTTCA

121 ACCGCTCATC TTGAGCAACA AGCCGTGGCA CCGGATCAAT ATCGTGCCGG CGCTGTGGCT

181 GGTCTTTGCA GTTACAGTGT CCCAACTCCA AGCATCACAC GCCGTGTGCA CTACAACGCA

241 GGTGTGTCAA CAGTCGAATC TGGCGCAGGC AGCGCTGACA AATTAGAGTC GTTCTACCAC

301 CACCGGATTC CAAGGGATGT GGCGCTGACG TCACTGCGCG CCGGAACAAT TAAGCATGAT

361 GTGGTGACAT TTGATGTGAG TGAGCGGGGA CCGACGCAGC CCCTCACCAA CCAGATTGTT

421 AATGTCACGT ACAAGCAGCA ATACAATTTC ATCGACGTGG GCGTGAGCGC AGCCATAGTC

481 AGAACTGAGC TTCAATTCTT CCCCGTCCGT CCACCTGTTG ATAACACGAA GCTGAACCTA

541 CTTAAGAATG CGCTGACTGT GCATGAAGTG GTGAAGCCTC TCAAACTACA ACTTCCCAAG

601 CTGGTGGCGC AATCGCACGC GTCGCCAGGC CTCCCGTTGT ACAGTGATTT CGAGCCACTG

661 CGGCGCGCGA TCGCGAACCA GGCCATAAAT GATGATTATG GCATGAGATT GTCACACATG

721 GCACGCATTG TGCTACACTC GTTCACTGCA CAAGCAGCCG GTTTATCACA CGCGGCTTCA

781 GATGTCGTGT CGACAGTTGA TGTTAGGACG ATGTTTGTGG CTGAAACGTA CCAGATGAGC

841 GCCGCCGAGT TGCAGCAGCG GGCGTTCGTG TATGTGGATG ATGACATTAG TGAAGAATAC

901 TGGGCCTTCC TGGCAATGGC AATGCTGGGC ATAAACACTA ATGTGGGTAA TACGCGCACC

961 GTATACTCAA GGCTAGTGTC GGTGTCAGAG TTGCGCGGCG GTCAGCAGCT ATACTACGTG

1021 ATGAAAAGCG GGGAGAACAC ACCGGCCAAC ATCAGCCAAA ATGCTTGCGC GTCGGTATTG

1081 AACAATCCTG AGAAATGCCT CTCATTCTAT AACGCCTATG CGCAATCATT AGGTTTAGCA

1141 CAAGAGGCGT CATCAGTCCT GCGCCAAGTT GCGGTGATGC CATTCATGTT TACTAAGAAC

1201 GCTACATTAC CGTACAAAGC ACGCGAGAAC CCCAAAGCTG ACGCATACGT GTACTTGCTC

1261 CGCGAGACTG CTGAGGACAT TCGATTGATG TACAATAGCG TTGAGACTCT GGTATATCAA

1321 GCCCCGATTA TCGCGGGCGC TGTTAAGGCA GGTGTGGCAG CGATATCTAT GTCGTACCGT

1381 GTCAAGAACT CGCTAAATGT GCCCTCCATA CTCGCAGCTA TCAAAGAGAT CCTCGCTGAC

1441 CCTAGTTCCT GCAGGAGCAC AATGTCAGCG GTGTTGTGCT CATACGGTGG TATCACGGCC

1501 GACTTGGAGT GGCTACTACC ATTCACAGAA GGAGTGGATA TAGCCATGGA TGCATGCGTG

1561 CGCGCCTACA GGGATTGCGG GTGGCTTATC ACGACTTACA ACGACTTGTC GTGCCTGGGT

1621 GCTTATGCTC CAGCATTTAG TACGGGTGTG GATATGCGCA ATGCACAATT CGGTTACGAC

1681 GTAGCGAGCA GACCGTATCT CGAGTCACTG ATCGCGCTGT CGACTCAAGG TTTACCGTGG

1741 ACGTTCGAAA ACGAATCTTT GGATGGACCA ACACTGCAAC GTGCCGCCAG GGACGTGGTG

1801 CACTGGGAGG CCGTAATTCA ATGGGATGCT TACTCACCAG GCGAAGGCGT AAGCGTGGCC

1861 CACATAACGC CAACCCCCGT CTCACCGAAA CAGGCCCGGC CTTTCGAGAC AGAGCCAAAC

1921 GCTCATCTGA GCGAACGGTG GCAAAAATCG AAGCGCACGC TGAGCGGTAA CGGCCTTAAA

1981 GCATTGACGC TGAGTCCACC ATCGGCTGCA GCACGCAGCG AAGCAACAAA AACGGCATCA

2041 GTAGCCGAAA CTCTGCGCAC ACACACGCGC TCAATGTCAG TCAAACCCAA CCCGGCACCC

2101 GGAACAAAGC CGCACAATAT GCCATGGCCA GTACAGGCGG CAGCCTCAGT ACCATCTGGC

2161 GAAGTTAGTG ATTCTGAGAG TACAACCACC CGAGTAACGC GGGCGAATTC AGTTGCATCA

2221 ACAGATGACA CGGCCAGTGC TGCTACGGTA GTTCAGACTG CCCCACCCGG CGTGACAAGC

2281 CCGCCGCGTG GCCGGTCCGT TACACCTATA GGTACGACTG TGGATTTGAG CCGTGAACCA

2341 ACGCCATCGC TGGCGGAACG GCTGAAGTCA CCATTCCGTC ACCAACGTGT TAGATCACGC

2401 AGTGTGAGTA GTAGTCGTGG TCGTGCGACG AGCGACACTA ACATGGGCCA AATGCTGGAC

2461 CGTGGCGCAA GTGAGTCGAG TGAGATAGCA CTAATACGGA AGACGAAGCC AAAGGACGTG

2521 GCTGAGCTAG GCGAACATAT TGTCACGAGC GACGGGCGAA TTGAGCTTAT AACGCAATTG

2581 GCGGGTAAAC GTGACTACAG TGATGTAGTG CGGGACCTAT TACCAGGCGG TGTAGGTACC

2641 CCAGACGTTG GCGATTGGAC GGGGACGAGT AAGAATGGTG AACAACGCAA TATCACAACG

2701 CTCATGAATG TGAACAAGTGATTTGGTTCG GAAATGCTTG TATAATTGTA TTACAGTTAT

2761 ACGAGGCCGG ATAGGCAAGC TAGCAGCAGT TCGGTACTAC CGTGGTGTTG CAAACCGCAT

2821 AAGGTATTAA AGTCATGGGA CTTTAATGGCGATAGGCAAG CTAGCAGCAG TTCGGTACTA

2881 CCGTGGTGTT GCAAACCGCA TAAGGTATTA AAGTCATGGG ACTTTAATGG C

FgV2 segment 2

1 GCAAAAAAGA GAAAAAGGAT CTTGACGCAC ATAGGTGCGC GGGGAGAAAC TTTCAAACCG

61 TGGCAAATTT GCACCCTTGT TCTAACATAC ATCATGTCAG GACAATTGAA CATTCGTTCA

121 ACCGCTCATC TTGAGCAACA AGCCGTGGCA CCGGATCAAT ATCGTGCCGG CGCTGTGGCT

181 GGTCTTTGCA GTTACAGTGT CCCAACTCCA AACATCACAC GCCGTGTGCA CTACAACGCA

241 GGTGTGTCAA CAGTCGAATC TGGCGCAGGC AGCGCCGACA AATTAGAGTC GTTCTACCAC

301 CACCGGATTC CAAGGGATGT GGCGCTGACG TCACTGCGCG CCGGAACAAT TAAGCATGAT

361 GTGGTGACAT TTGATGTGAG TGAGCGGGGA CCGACGCAGC CCCTCACCAA CCAGATTGTC

421 AATGTCACGT ACAAGCAGCA ATACAATTTC ATCGACGTGG GCGTGAGCGC AGCTATAGTC

481 AGAACTGAGC TCCAGTTCTT CCCCGTCCGC CCACCAGTTG ATAACACGAA GCTGAACCTA

541 CTTAAGAATG CGCTGACTGT GCATGAAGTG GTGAAGCCTC TCAAACTACA ACTTCCCAAG

601 CTGGTAGCGC AATCGCACGC GTCGCCAGGC GCACAGTTAT ACAGTGATTT CGAGCCATTG

661 CGGCGCGCGA TCGCGAACCA GGCCATAAAT GATGATTATG GCATGAGGTT GTCACACATG

721 GCACGCATTG TGCTACACTC GTTCACCGCA CAAGCAGCCG GTTTATCACA CTCGGCTTCA

781 GATGTCGTGT CGACAGTTGA TGTCAGGACG ATGTTTGTGG CAGAAACGTA CCAGATGAGC

841 GCCGCCGAGT TGCAGCAGCG GGCGTTCGTG TATGTGGATG ATGACATTAG TGAAGAATAC

901 TGGGCCTTCT TGGCGATGGC AATGCTGGGC ATAAACACTA ATGTGGGTAA TACGCGCACC

961 GTATACTCGA GGCTAGTGTC GGTGTCAGAG TTACGCGGCG GTCAACAGCT ATACTACGTG

1021 ATGAAAAGCG GGGAGAACAC ACCGGCCAAT ATCAGCCAAA ATGCTTGCGC GTCGGTGTTG

1081 AACAACCCCG AGAAATGCCT CTCATTCTAT AACGCCTATG CGCAATCATT AGGTTTAGCA

1141 CAAGAGGCGT CATCAGTCCT ACGCCAAGTT GCGGTGATGC CATTCATGTT CACTAAGAAC

1201 GCTACATTAC CGTACAAAGC ACGCGAGAAC CCCAAAGCTG ACGCATACGT GTACTTGCTC

1261 CGTGAGACTG CTGAGGACAT CCGATTGATG TACAACAGCG TTGAGACTCT GGTGTATCAA

1321 GCCCCAATTA TCGCGGGCGC CGTCAAGGCA GGTGTGGCAG CGATATCTAT GTCGTACCGT

1381 GTCAAGAACT CTCTAAATGT GCCCTCCATA CTCGCAGCTA TCAAAGAGAT CCTCGCTGAT

1441 CCTAGTTCCT GCAGGAGCAC AATGTCAGCG GTGTTGTGCT CATACGGTGG TATCACGGCC

1501 GACTTGGAGT GGCTGTTACC ATTCACAGAA GGGGTGGATA TAGCCATGGA TGCATGCGTG

1561 CGCGCTTACA GAGATTGCGG GTGGCTTATC ACCACCTACA ACGACTTGTC GTGCCTGGGT

1621 GCTTATGCTC CAGCATTTAG CACAGGTGTG GATATGCGCA ACGCGCAATT TGGTTACGAC

1681 GTAGCGAGCA GACCGTATCT CGAATCACTG ATCGCGCTGT CGACTCAAGG TTTACCATGG

1741 ACGTTCGAAA ACGAATCTTT GGATGGACCA ACACTGCAAC GTGCTGCTAG GGACGTGGTG

1801 CACTGGGAGG CCGTAATTCA ATGGGATGCT TACTCACCCG GCGAAGGCGT AAGCGTGGCC

1861 CACATAACAC CAACCCCCGT CTCGCCAAAA CAGGCCCGGC CTTTCGAGAC AGAGCCAAAC

1921 GCTCATCTGA GCGAACGGTG GCAAAAATCG AAGCGCACGC TGAGCGGTAA CGGCCTCAAA

1981 GCATTGACGC TGAGTCCACC ATCAGCTGCA GCACGCAGCG AAGCAACGAA AACGGCAACA

2041 GTAGCTGAAA CCCTGCGCAC ACACACGCGC TCGATGTCAG TTAAGCCCAA CCCAGCACCT

2101 GGAACAAAGC CTCACAGTAT GACATGGCCA GTACAGGCGG CAACCTCAGT ACCATCTGGC

2161 GAAGTTAGTG ATTCTGAAAG TACAACCACC CGAGTAACAC GGGCGAATTC AGTTGCATCA

2221 ACAGATGACA CTGCCAGTGC TGCTACGGTA GTTCAGACTG CCCCACCCGG CGTGACAAGC

2281 CCGCCGCGCG GCCGGTCTGT CACACCTATA GGCACGACTG TGGATTTGAG CCGTGAACCA

2341 ACGCCATCGC TGGCGGAACG GCTGAAATCA CCATTCCGTC ACCAACGTGT TAAATCACGC

2401 AGTGTGAGTA GTAGTCGTGG TCGTGCAACG AGCGACACTA ACATGGGCCA AATGCTGGAC

2461 CGTGGCGCAA GTGAGTCGAG TGAGATAGCA CTGATACGGA AGACGAAGCC AAAGGACGTA

2521 GCTGAGCTAG GCGAACATAT TGTCACGAGC GACGGGCGAA TTGAGCTTAT AACGCAATTG

2581 GCGGGTAGAC GTGACTACAG TGATGTAGTG CGGGACCTGT TACCAGGCGG TGTAGGCACC

2641 CCAGACGTTG GTGATTGGAC GGGGACGAGC AAGAATGGTG AACAACGTAA TATCACAACG

2701 CTCATGAATG TGAACAAGTG ATTTGGTTCG AAAATACTTG TGTAATTGTA TTACAGTTAT

2761 ACGAGGCCGG ATAGGCAAGC TAGCAGCAGT TCGGTACTAC CGTGGTGTTG CAAACCGCAT

2821 AAGGTATTAA AGTCATGGGA CTTTAATGGCGTCATGAATG TGAACAAGTG ATTTGGTTCG

2881 AAAATACTTG TGTAATTGTA TTACAGTTAT ACGAGGCCGG ATAGGCAAGC TAGCAGCAGT

2941 TCGGTACTAC CGTGGTGTTG CAAACCGCAT AAGGTATTAA AGTCATGGGA CTTTAATGGC

FgVCh9 segment 3

1 GCAAAAAAGA AAAGAAGGGT AAGAGTACCT CAACCGCACA TAGGTGCGCG GGGAGAAATT

61 TTCAAACCGT GGCAAATTTG CACAAACCAT ATTTGATTTA CAAATATGGC ATCGAACGCA

121 TTGTTCGACA GACGTGGTGG TGGTGAGCAA GGTGTTAGTG GCAACAACGG AAGTTCTGCG

181 GGGCGCACCG TTAAGCCAGC ATTCCAGGGG GATGATTTCT TTGGAATAGC TTCTGCGCTG

241 GAGATGAACC CAAACATGGG TGTAAACGAC ATGATGCTTG TTGGTCGGGA TAAGCTAATG

301 TCGGGGAAGC AGCAAGCAAA CCCCGGCCTA GTGGGATGGC AGCACGATAT GCAGCGCAAC

361 CTGGCCACAG ACGCATCAGC CTGGGTCGTG CGCAAGGAAA TACGAGACCT GGCTGGCCTC

421 CTACCTGATA ACCCCAATGA TGACACAGCG AGACTGGAGA CCGTGGTCAC GTTCACATAC

481 CAGGTCGAAC CCGTCAACCA GCGAGCTGAT CGGTCACACC GACGCGATGT GGCTGTGCAA

541 TACAAAGCTC CTTCAGTGGG CGATGGTACA GTCATCCAGT TCGCTAAATC CACCAAAGAG

601 TTGCACTACA AGGCGAGCGC AGCTACAGCG TCGTCGGCCG CTATAGGGTA TGCTCAGCAG

661 GTGAATTCCT ATTCCCGGAC TGGCATCTCC GGTGACATGG GCCTGGCAAT GGAAGTTGGT

721 CGTGGACCGG ATATGCTGGG TGTGTTCACG GTGATGTGGC TGTTGCAACT TGATGTGGCG

781 GGTGGAGCAC GTGTGGAGCT CGGCATAAAC GCGAATGATA TGCGCGTGCT GGTGCCATCA

841 TCGGTGTCAC CCCTGGATGA AGCTGACGCA ATGAAGGCAG TGCCCGGCAT CAGGCTACAT

901 TCAGACGCCC TCGACCCTCT AGCATACCGG CTGCTATACA CTGCAACGCA CCGGTCAGCC

961 CTTGGCGCCG CATCTCCGTG GGTCAACAGA TATCGCTGGC CAACCACACC GCTAACTGTG

1021 TATAGCGGCG CACTAAACCC GGGACCGTAC ATGCAAGCAT TCAATGGTAG GCCTGCAGCT

1081 GCGCAGCTAG CCGGGCAGAT TGTCAACATT GCGAACACGT ATGACCTGCG TGACACGTGC

1141 AGTCGTGCTT ACAGCATTGC ATTCTCTCTT TACGGTGATC GTTGTGTGAA CAGGAATTTC

1201 CGCCTGCACT GCGGTAGGCA GACACTGTTC CATGAAATCA GTAATGGGCA GAGCAAAGCA

1261 CAGGTGCCTC AAATGTGTTG GGCCCTCGGT AGTTACCAGC TGCCTATGGC GGCAAAGTTC

1321 AATGGGTATG CGAACGCAGA GTACATGCGC GACCTGTGTC GTTCTACGAT AACTGCTGGC

1381 TTGCGCATCA GGGACCGCGA CGCTGTCCGA CGGGCCGTTG ACCAAATCGA TGTGCATGCC

1441 CTCAAAACTG TGCAGCAACG TTTTGAGGAA GGACAGCAAC TAACGGATGC ATTGCCAATA

1501 GACTACAGGC ATTATGTTAA GCATGGCATC CGACGCGCCT GGGTCGTTCG AGGCATCTTG

1561 CACAATCTCT CGCTCGGCCG TGCTGTGCCG GCAGCGATCA GCGGCTCAGC AGTTAAACAA

1621 TTACGCATGC CAAGTGAAGC AGAACTGGCG GACACCGACG TGCTGGCGTA TGGGAAGGAG

1681 CGTGCGGAGT CAGAAGCAAA TGCGTGGCTC ATAAATGAAC TGATACGAAA CGGTGGTACT

1741 AGCTTATGCC AGAGTGTAGA TGAGGCCATA CCACGATCTG GCGCAACAGC AGTGTTGCAC

1801 ACCGATTGCC GTTATTACAT GGGCATGGAT CTACAATACA AGATTGTTGG ATTCGGTAAT

1861 GATATGCGCA TAAAGTACAA CAGGCCGCTC AACTTTGTGT ATGCACCTAC ACCCACAATG

1921 GTAACACACC AGCGTGATGA TCAACTGCGT GAACCAAGTG CGCTACCGAT GACTGTGGTA

1981 GACAAGGAGC TAGCAGATGA CAAAGCTGAC GACACTGTTG TGACCCCGGA AGATATACTG

2041 CGTGAGGTAA TGCAGATGCG CGGCACTCGC ACGTCAACGT GGTTCAGGGG GAAACCACCC

2101 ACAAAAGCGC CGCCGGTTCG GTACCACGAT AGTGACGTTT TGACCGTGAC AGCTGCCCGT

2161 GATGATGTAC GCCAATCTGT GGTCCAACTA GCCCAAGAAG GTGGTGGTCC TGGACCCACC

2221 AGCGGTGAAA ATCTGCTGTG TGGTGCTCAT GCGATACAGA TGGCGCTAGA ATTCGCAGGT

2281 CACCCCAACC ACATAACAGT GGAACAGTTG GAGGATGAGC TGCGCACTGC CGTGCTCGAT

2341 GCACCTGTGG ATGATGACTC GGAATTGCGG AGTGCAAACA ATTTCACGGC AGACCAATTG

2401 GGCAGAGTAG CTAATCGGCA CGGGTATAAT CTGACAGTCG TGACAGGACA AGGCAAAGGT

2461 GCCGCACTGG CATACAGCGC AACAGAGCTG GTCGGTGCAC CCCACCTTGT TGTGTTCAAC

2521 TTCGGAGGCA ATCACTGGGA AGGTCCGGTC CGGCAAGGTA AGCGTCATGC AATACGAGTA

2581 GAAGGTCAAG ATGCAGGTAC GCCAGCGGTA GAAGCGGCAC GGGCCCGGTC CCAGCGTGGC

2641 AGCGTGTTTG AGAAGACCGT GAACCGTCGA CGTTAAATCA CAAACAATAA ATAGTTAATT

2701 GGTATTACCG GTTAGTCTAT TGCCAAATTG GCGCCAGTAG CAACACACTC GCACGCTACG

2761 CCACAGTCTG TACAGCACTG CTGCCCAGAA TGTCGCCAAA TTAGGTATTT AAGGAGAACC

2821 CTTAAATGGCACGAACCGTC GACGTTAAAT CACAAACAAT AAATAGTTAA TTGGTATTAC

2881 CGGTTAGTCT ATTGCCAAAT TGGCGCCAGT AGCAACACAC TCGCACGCTA CGCCACAGTC

2941 TGTACAGCAC TGCTGCCCAG AATGTCGCCA AATTAGGTAT TTAAGGAGAA CCCTTAAATG

3001 GC

FgV2 segment 3

1 GCAAAAAAGA AAAGAAGGGT AAGAGTACCT CAACCGCACA TAGGTGCGCG GGGAGAAATT

61 TTCAAACCGT GGCAAATTTG CACAAACCAT ATTTGATTTA CAAGTATGGC ATCAAACCCA

121 TTGTTCGACA GACGTGGTGG TGGTGAGCAA GGTGTGAGTG GCAACAACGG CAGTTCCATG

181 GGGCGCACCG TTAAGCCAGC ATTCCAGGGG GATGATTTCT TTGGAATAGC CTCTGCACTG

241 GAGATGAACC CAAACATGGG TGTAAACGAC ATGATGCTTG TTGGTCGGGA CAAGCTGATG

301 TCGGGGAAGC AGCAGGCAAA CCCCGGCCTA GTAGGATGGC AGCACGATAT GCAGCGCAAC

361 CTAGCTACAG ACGCATCGGC CTGGGTCGTA CGCAAGGAAA TACGAGATCT AGCTGGCCTT

421 CTACCTGACA ACCCCAACGA TGACACAGCG AGACTAGAGA CCGTGGTCAC GTTCACATAC

481 CAGGTCGAAC CTGTTAACCA GCGGGCTGAT CGATCACACC GCCGCGATGT GGCTGTGCAA

541 TACAAAGCCC CCTCGGTAGG CGATGGTACA GTCATCCAGT TCGCTAAATC CACTAAAGAG

601 TTGCACTACA AGGCGAGCGC AGCTACGGTG TCATCCGCCG CTATAGGGTA TGCTCAGCAG

661 GTGAATTCCT ATTCCCGGAT TGGCATCTCT GGTGACATGG GCCTGGCAAT GGAGGTTGGT

721 CGTGGACCGG ATATGCTGGG TGTGTTCACG GTGATGTGGC TGTTGCAACT CGATGTGGCG

781 GGTGGAGCAC GTGTAGAGCT CGGCATAGAC GCGAATGATA TGCGCGTGCT AGTGCCATCA

841 TCAGTGTCAC CCCTGGACGA AGCTGACGCA ATGAAGGCAG TGCCCGGCAT CAGGCTTCAT

901 TCAGACGCCC TCGACCCTCT AGCATACCGG CTGCTGTACA CTGCGACGCA CCGATCAGCC

961 CTTGGCGCCG CATCTCCGTG GGTCAACAGG TATCGCTGGC CAACCACACC GCTAACTGTG

1021 TATAGCGGCG CACTAAACCC GGGACCGTAT ATGCAAGCAT TCAATGGTAG GCCTGCAGCT

1081 GCACAGCTAG CCGGGCAGAT TGTTAACATT GCGAACACGT ATGACCTGCG TGACACGTGC

1141 AGTCGTGCTT ACAGCATCGC ATTCTCTCTT TACGGTGATC GCTGTGTGAA CAGGAATTTC

1201 CGCCTGCACT GCGGTAGGCA GACACTGTTC CATGAAATCA GTAATGGGCA GAGCAAAGCA

1261 CAGGTGCCTC AAATGTGTTG GGCCCTCGGT AGTTACCAGC TGCCCATGGC GGCAAAGTTC

1321 AACGGGTATG CGAACGCAGA GTACATGCGC GACCTGTGCC GCTCTACGAT AACCGCTGGC

1381 TTGCGCATCA GGGACCGTGA CGCTGTCCGA CGGGCCGTTG ACCAAATCGA TGTGCATGCC

1441 CTCAAAACTG TGCAGCAACG CTTTGAGGAA GGGCAGCAAC TAACGGGTGC GCTACCAATA

1501 GACTACAGGC ATTATGTTAA GCATGGCATC CGACGTGCTT GGGTCGTTCG AGGTATCTTG

1561 CACAACCTCT CACTTGGCCG TGCTGTGCCA GCAGCGATCA GCGGCTCAGC AGTCAAACAA

1621 TTACGCATGC CAAGTGAAGC AGAACTAGCG GACACTGACG TGCTGGCGTA TGGGAAAGAG

1681 CGTGCAGAGT CAGAAGCAAA TGCGTGGCTC ATAAATGAGC TGATACGAAA CGGTGGCACT

1741 AGCTTATGCC AGAGTGTAGA TGAGGCCATA CCACGATCTG GCGCAACCGC GGTGCTACAC

1801 ACCGATTGCC GTTATTACAT GGGCATGGAT CTGCAATACA AGATTGTTGG ATTCGGTAAT

1861 GATATGCGCA TAAAGTACAA CAGGCCGCTC AACTTTGTGT ATGCACCTAC ACCCACAATG

1921 GTAACACACC AGCGTGATGA TCAACTGCGT GAACCAAGTG CACTACCAAT GACTGTGGTG

1981 GACAAGGAGC TAGCAGATGA CAAAGCTGAC GACACTGTTG TGACCCCGGA AGATATACTG

2041 CGTGAGGTAA TGCAGATGCG CGGCACTCGC ACGTCAACGT TATTCAGGGG AAAACCACCC

2101 ACAAAAGCAC CGCCTGTCCG GTACCACGAT GGTGACGTTT TGACTGTGAC CGCCGCCCGT

2161 GATGATGTGC GCCAATCTGT GGTCCAACTA GCCCAGGAAG GTGGTGGTCC GGGACCCACC

2221 AGCGGTGAAA ATCTACTGTG TGGTGCTCAT GCGATACAGA TGGCGCTAGA ATTCGCAGGT

2281 CACCCCAACC ACATAACAGT AGAACAGTTG GAGGATGAGC TGCGCACTGC CGTGCTCGAT

2341 GCACCCGTGG ATGATGACTC GGAATTGCGG AGTGCAAACA ATTTCACGGC AGATCAATTG

2401 GGCAGAGCAG CTAATCGACA CGGGTATAAT CTGACAGTCG TGACAGGGCA AGGCAAAGGT

2461 GCCGCAGTGG CATACAGCGC AACGGAGCTG GTCGGTGCAC CCCACCTTGT TGTGTTCAAC

2521 TTCGGAGGCA ATCACTGGGA AGGTCCAGTC CGGCAGGGTA AGCGTCATGC AATACGAGTA

2581 GACGGTCAAG ATGCAGGTAC GCCAGCGGTG GAAGCGGCAC GAGCCCGGTC CCAGCGTGGC

2641 AGCGTGTTTG AGAAGACCGT GAACCGTCGA CGTTAAATCA TAAACAATAA ATAGTTAATT

2701 GGTATTACCG GTTAATCTAT TGCCAAATTG GCGCCAGTAG CAACACACTC GCAAGCTACG

2761 CCACAGTCTG TACAGCACTG CTGCCCAGAA TGTCGCCAAA TTAGGTATTT AAGGAGAACC

2821 CTTAAATGGC ACTCATGAAT GTGAACAAGT GATTTGGTTC GAAAATACTT GTGTAATTGT

2881 ATTACAGTTA TACGAGGCCG GATAGGCAAG CTAGCAGCAG TTCGGTACTA CCGTGGTGTT

2941 GCAAACCGCA TAAGGTATTA AAGTCCCATG ACTTTAATGG CG

FgVCh9 segment 5

1 GCAAAAAAGA GAAAAAGCCA CAAACCGCAC ATAGGTGCGC GGGGAGAAAT TTTCTAACTG

61 CAGCTAATTA GCATAACATA CCAGCAATTC GCCGATATGT ATTCGCACTC AAGTTCAAGT

121 GCACAACGCG CAGCTGTTTG GGCGCAACAA CAGCGAACCG TGGCCTTTAC GGCCCATCAA

181 TCAAACTTTA CCGACGTGGC ACTGGGCACA CCACGCCATA CCATGGTGGC TCAGCCCCGG

241 AATGGTGTGG CATCTAGGGA AAATCATGCT CAGCAGTGGT CTGATGAGCG GATTCCAACT

301 GGGCCAGGGT ATGACCTGGT TAACCGCTTC GAGATGATTG CAGACCGTGA GCTTGAGCGT

361 GCTGGGTTGG CAGAGCCACG CACAACGCAC TATGGTGAGG GCGCTGCTAG TTCGGCAAGT

421 TCGACCACGA GCGCTAGACA TTCGTTATTT AGCGTGCAAC CTCAAAGCAC CACTGGTTGT

481 GCTTCGAGTA CTAACGGAGA GAGCGACGAT GATGGAGGCT ATGACTACGA GAGCGATAGT

541 AGTTATGACG ATGATGCACG CTCGGCAGGC GATGACACCT TGTATAACAT GCGTGCACAC

601 ACCCAGTACC AGCATGAGCT GCACGAAGAG AACCTGCTTG ATGAGGCAGA TGATTACATC

661 CATTCTGCTG CACTCACCCG GTCCACACCC CCGCTCTGGT TAGCCGACAC CTACCCGATA

721 ACTGAGCCAG CTATTGAAGC CCGGTACGAC GTTTATTACG ATCAGTACCA CAGTGTAACC

781 ACCCCAATTT ATCCAGGACC AGGTGCAACA CTGCACGAAG ATTTGGTGCT TGACGCCCGG

841 CCCTTTGTTT GCCATAAACC CCTGACCCAG CCAGCACCGA TCGCGTCTCG TACTGGGTCA

901 CTTGAGTATC AGCTAGAATT GCAAGTCGCT GCTATGGCAG CGGGGGACAC GGATTTGGTC

961 GTTAACATGA TCAAGCTCGA ACGATCAAGC AGCCCCGCAT CACCACAGGA TCGGCAGTCT

1021 AGGCACGGTG AGAACCAAGT TGCTGAAGCA TCTGGTCACA GACACCCTCT GCCGGACCTG

1081 GCATGCCCGA TTTGCTTCGA GCCACAACGT TCGCGACGTT CACTTGCCGC CCACCGGAGC

1141 TCATCGCACC CTAAACAAGT ATGTGGTCAG GCGCACTGCA GCTACACTAC TGACGTGCCG

1201 GCTGACTTTG CTTTCCATAT GGATGAAGAA CACCCTGTCT TAGGGTGTGA TTCAACAGGC

1261 TGCGATTACA CTACACGCAG CAGCCAACTA CACTGGACTC ATATGGTTGG GCACCTGAGT

1321 TGCACAGTGT GTGGGCTGCT AAGTGCCAAC CTCGCAGCGG CTGCTGATCA CATTCGCGAA

1381 CATGCTGGAA AGTGGTCCTG TTCACTGTGC AACGAGCCCT TCGAGGACGC GTTCAGCCTT

1441 AATGAGCATA ATCAGGATGT GCACTTTACC TGCTGCACTC AATGTGAAGC CATTGTAAGA

1501 GACAAGAACG AACTACTGGA CCACTGCCAT GATGTTCACC CGTACTTACG GTGTCGTGAA

1561 GTAGGCTGCG CGTTCCGCAC ATTTGAACCA CCGGAGCTAG CAGCACACCA TGATTGGCAT

1621 GATCAATACC CATTGTCGTT CTTCGTGGTG AAGTTGCAAT GCCCTGACCT CGGTTGCACT

1681 CATGCGGCAT CAAGTCATGA GATGGCAGCG CTACATTACA ACACTCACTT GCAGAAACCG

1741 AATTACGCGT GTGAAAGTTG TGACGCTTGG TGCCACAGCC ACACTGCCCT AATCAGGCAC

1801 TCCCGGATGC ACAAGCAGGT AGAGCAGAAT CTGCGAGAAC AGCCCGCACT CCAACAAGCA

1861 CCTGCAACGG CGTCTGGCGA TGTGTGCAAA TGTCCACATT GTGCGTTCAC GAGTAAGTCA

1921 AAAGTTGAAT GGCAAGATCA TATCAACATC AATCACCGTT CATCCCAACA TCTAGCATTA

1981 CCTTGTAGCT TATGCGGCAA GGTGTTCACT GGCGTGAATG CGGTCCGGAA ACATCACTGT

2041 CACAAACGTG ATTGGCATGA TAAACCGCAC CGTTGTGGTA AATGTAATGC ACGATTTGGT

2101 CAGTCTCGCG ACCTAGAGCG CCATAAAGCG ACACATGAGG CAGCTAGGCC CCGACACCAA

2161 TGTCAGGTTT GCCACGAAAC ATTCGCGCGG CTGGACCATT TGCAACGCCA CGTTAGACGG

2221 CGGCACTCCC GGATGCACAA GCAGGTAGAG CAGAATCTGC GAGAACAGCC TGCACTCCAA

2281 CAAGCACCTG CAACGGCGTC TGGCGATGTG TACAAATGTC CACATTGTGC GTTCACGAGT

2341 AAGTCAAAAG TTGAATGGCA AGATCATATC AACATCAATC ACCGTTCATC CCAACATCTA

2401 GCATTACCTT GTAGCTTATG CGGCAAGGTG TTCACTGGCG TGAATGCGGT CCGGAAACAT

2461 CACTGTCACA AACGTGATTG GCATGATAAA CCGCACCGTT GTGATAAATG TAATGCACGA

2521 TTTGGTCAGT CTCGCGACCT AGAGCGCCAT AAAGCGACAC ATGAGGCAGA TAGGCCCCGA

2581 CACCAATGTC AGGTTTGCCA CGAAACATTC GCGCGGCTGG ACCATTTGCA ACGCCACGTT

2641 AGACGGCGAC ATACATAATG GCACCACACT GGTACGTTGG CATACTTGTG GTATTACCGC

2701 AGTACTTGCC ACCCTAATTT GGGATGCTGC TAGCATGTAA TGGCACCACA CTGGTACGTT

2761 GGCATACTTG TGGTATTACC GCAGTACTTG CCACCCTAAT TTGGGATTGC TGCTAGCAAT

2821 GCATGAGTAC CAGTAACATA CTGCCGGACA CAACAGCCAA ACACCTGTTT TGGCACCAGG

2881 CAGTATGGTA ATTAATAATT ATTTAGGCTT GGCCTACCCC TAAATGGC

The sequence, which is duplicated, is underlined. The duplications themselves are dotted underlined. Start and stop codons are shown in bold letters. Nucleotides, which connect the duplications or are inserted (T in FgVCh9 segment 5) are shown in boxes.

In segment 3 of FgV2 the underlined sequence is a duplication of the 151 3´ terminal bp of segment 2 of this virus, however with the exception of 4 bp (bold italics) in the extreme end.

The internal duplication in segment 5 of FgV-ch9 is composed of a 5´and 3´ duplicated sequence (underlined), which are both connected by non-homologous nucleotides (shown in boxes).

Supplement 2B

1 MYSHSSSSAQRAAVWAQQQRTVAFTAHQSNFTDVALGTPRHTMVAQP