INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC)
987830 Nebraska Medical Center
Omaha, NE 68198-7830
402/559-6463
Fax 402/559-3300
Addendum to Experimental Application-Breeding Procedures
Instructions: This addendum to be completed only if you are proposing to breed animals that would produce pregnant females and/or suckling pups to be used for experimental purposes and/or blood/tissue collection on this specific protocol. This addendum is to address breeding procedures/ colony management. Ensure that all experimental work that will be done on bred animals and/or pups are covered under the main application.
Accurate records of the number of animals produced and their ultimate disposition (i.e. used in experiments, euthanized, died or retained for future breeding stock) should be maintained in your laboratory.
1. Justification for the Need of Breeding Animals. Provide a scientific justification for the need to breed animals. Include the following information in the justification: how breeding animals contributes to the overall objectives of your research and an explanation of why animals from commercial vendors cannot be used. Note: Cost alone is not considered a justification for breeding.
2. Breeding Scheme to be Used. Select all that apply.
Monogamous
Harem
Trio
Polygamous
Timed mating
Other:
3. Weaning Schedule. Select at what age the animals will be weaned.
21 days of age or less
Older than 21 days
a. Indicate at what age the animals will be weaned:
b. Justify the need for delayed weaning:
4. Breeding Stock Selection. Describe the criteria you will use to select the future breeding stock.
5. Experimental Animal Selection. Describe the criteria you will use to select which animals are used for experiments.
6. Breeding Colony Maintenance. For purposes of CM management, how long do you intend to maintain the breeding colony?
7. Cage Density Management. Space requirements for breeding mice are outlined in the Policy for Cage Population Densities for Breeding Mice.
I assure the IACUC that I will adhere to the Policy for Cage Population Densities for Breeding Mice.
I request an exception to the Policy for Cage Population Densities for Breeding Mice.
Explain:
Not applicable, not a mouse protocol.
8. Animal Identification. Policy for Rodent Identification SOP on Rodent Identification Techniques
a. How will you ID the animals? Check all that apply.
Standard cage cards
Ear notch
Ear tag
Tattoo
Microchip
Other:
Toe Clip
Provide scientific justification for the use of toe clipping vs other ID methods:
Toe Clip-within the first 7 days of life
Toe Clip-after the first 7 days of life. Requires the use of anesthesia.
Indicate what anesthetic/s will be used:
Agent / Dose (mg/kg) / RouteAddendum Breeding Procedures_7.10.2013
b. Will you follow the SOP on Rodent Identification Techniques?
Yes
No, I request a deviation from the SOP on Rodent Identification Techniques.
Explain:
9. Animal Genotyping. Will you genotype the animals? Policy for Rodent Genotyping SOP on Rodent Genotyping Techniques
No Genotyping is not necessary.
Yes
a. Methods of genotyping. Check all that apply.
Ear punch
Hair bulb
Fecal pellet
Buccal swab
Other:
Blood Sample-Describe method:
Will any sedatives, anesthesia, and/or analgesics be used to provide pain control and/or immobilization for the blood collection procedure?
No The blood collection procedure does not require sedatives, anesthesia or pain control.
Yes Intra-procedural drugs will be used. Indicate what agents will be used.
Addendum Breeding Procedures_7.10.2013
Agent / Dose (mg/kg) / RouteAddendum Breeding Procedures_7.10.2013
Tail Biopsy
Single Tail Biopsy-within the first 21 days of birth
Single Tail Biopsy- greater than 21 days of age
Provide scientific justification for the use of tail biopsy on animals greater than 21 days of age:
Indicate what anesthetic/s will be used:
Agent / Dose (mg/kg) / RouteAddendum Breeding Procedures_7.10.2013
Multiple Tail Biopsies
Provide scientific justification for the use of multiple tail biopsies:
Indicate what anesthetic will be used:
Agent / Dose (mg/kg) / RouteAddendum Breeding Procedures_7.10.2013
b. Incorrect genotype. What will be the disposition of the animals that do not have the desired genotype?
c. Will you follow the SOP on Rodent Genotyping Techniques?
Yes
No, I request a deviation from the SOP on Rodent Genotyping Techniques.
Explain:
Addendum Breeding Procedures_7.10.2013