Online Resource 2Species-level clustering of ciliate OTUs. The OTUs were clustered based on primer pair D amplicon of 137 previously reported ciliates 18S rRNA gene sequences using different cutoff values in MOTHUR.

Species / cutoff value
0.010 / 0.015 / 0.019 / 0.026 / 0.030 / 0.036 / 0.041
Balantidium coli / 8 / 9 / 9 / 8 / 7 / 7 / 7
Balantidium coli / 8 / 9 / 9 / 8 / 7 / 7 / 7
Balantidium coli / 8 / 9 / 9 / 8 / 7 / 7 / 7
Balantidium coli / 8 / 9 / 9 / 8 / 7 / 7 / 7
Balantidium coli / 8 / 9 / 9 / 8 / 7 / 7 / 7
Balantidiumctenopharyngodoni / 51 / 45 / 40 / 33 / 26 / 23 / 20
Balantidiumentozoon / 50 / 51 / 45 / 34 / 25 / 20 / 21
Blepharocoryscurvigula / 21 / 22 / 22 / 21 / 12 / 13 / 11
Blepharocorys sp. NM-2008 / 21 / 22 / 22 / 21 / 12 / 13 / 11
Blepharocorysuncinata / 52 / 44 / 42 / 31 / 23 / 24 / 23
Parentodinium sp. YM2009 / 53 / 46 / 39 / 30 / 24 / 27 / 18
Bundleiabenbrooki / 55 / 49 / 41 / 32 / 29 / 21 / 19
Bundleia nana / 57 / 52 / 47 / 37 / 30 / 22 / 12
Bundleiapostciliata / 60 / 50 / 46 / 38 / 31 / 26 / 14
Cochliatoxumperiachtum / 63 / 47 / 44 / 22 / 15 / 8 / 4
Cycloposthiumbipalmatum / 13 / 11 / 12 / 2 / 3 / 3 / 3
Cycloposthiumedentatum / 13 / 11 / 12 / 2 / 3 / 3 / 3
Cycloposthiumishikawai / 13 / 11 / 12 / 2 / 3 / 3 / 3
Cycloposthium sp. / 54 / 11 / 12 / 2 / 3 / 3 / 3
Dasytricharuminantium / 9 / 7 / 8 / 7 / 6 / 6 / 6
Dasytricharuminantium / 48 / 7 / 8 / 7 / 6 / 6 / 6
Dasytricharuminantium / 9 / 7 / 8 / 7 / 6 / 6 / 6
Dasytricha sp. LDK-2011 / 9 / 7 / 8 / 7 / 6 / 6 / 6
Dasytricha sp. LDK-2011 / 9 / 7 / 8 / 7 / 6 / 6 / 6
Dasytricha sp. LDK-2011 / 9 / 7 / 8 / 7 / 6 / 6 / 6
Anoplodiniumdenticulatumdenticulatum / 4 / 4 / 4 / 3 / 1 / 1 / 1
Anoplodiniumdenticulatummonacanthum / 49 / 4 / 4 / 3 / 1 / 1 / 1
Diplodiniumdentatum / 4 / 4 / 4 / 3 / 1 / 1 / 1
Diplodiniumdentatum / 4 / 4 / 4 / 3 / 1 / 1 / 1
Diplodiniumdentatum / 4 / 4 / 4 / 3 / 1 / 1 / 1
Diplodiniumdentatum / 4 / 4 / 4 / 3 / 1 / 1 / 1
Diplodiniumdentatum / 4 / 4 / 4 / 3 / 1 / 1 / 1
Diplodiniumdentatum / 4 / 4 / 4 / 3 / 1 / 1 / 1
Diploplastron affine / 20 / 14 / 11 / 3 / 1 / 1 / 1
Diploplastron affine / 20 / 14 / 11 / 3 / 1 / 1 / 1
Enoploplastrontriloricatum / 58 / 20 / 24 / 13 / 14 / 1 / 1
Enoploplastrontriloricatum / 47 / 20 / 24 / 13 / 14 / 1 / 1
Entodinium bursa / 59 / 2 / 2 / 5 / 2 / 2 / 2
Entodiniumcaudatum / 3 / 2 / 2 / 5 / 2 / 2 / 2
Entodiniumcaudatum / 3 / 2 / 2 / 5 / 2 / 2 / 2
Entodiniumcaudatum / 3 / 2 / 2 / 5 / 2 / 2 / 2
Entodiniumcaudatum / 62 / 2 / 2 / 5 / 2 / 2 / 2
Entodiniumcaudatum / 3 / 2 / 2 / 5 / 2 / 2 / 2
Entodiniumdubardi / 27 / 27 / 15 / 10 / 2 / 2 / 2
Entodiniumfurcadilobum / 19 / 18 / 15 / 10 / 2 / 2 / 2
Entodiniumfurcamonolobum / 3 / 2 / 2 / 5 / 2 / 2 / 2
Entodiniumfurcamonolobum / 24 / 16 / 17 / 16 / 2 / 2 / 2
Entodiniumlonginucleatum / 33 / 28 / 27 / 27 / 2 / 2 / 2
Entodiniumnanellum / 32 / 29 / 23 / 14 / 9 / 10 / 2
Entodinium simplex / 31 / 30 / 23 / 14 / 9 / 10 / 2
Entodinium sp. LDK-2011 / 3 / 2 / 2 / 5 / 2 / 2 / 2
Entodinium sp. LDK-2011 / 3 / 2 / 2 / 5 / 2 / 2 / 2
Entodinium sp. LDK-2011 / 30 / 31 / 21 / 5 / 2 / 2 / 2
Entodinium sp. LDK-2011 / 29 / 32 / 21 / 5 / 2 / 2 / 2
Entodinium sp. LDK-2011 / 19 / 18 / 15 / 10 / 2 / 2 / 2
uncultured rumen Ophryoscolecidae / 24 / 16 / 17 / 16 / 2 / 2 / 2
uncultured rumen Ophryoscolecidae / 28 / 36 / 29 / 24 / 9 / 10 / 2
Eremoplastrondilobum / 35 / 38 / 11 / 3 / 1 / 1 / 1
Eremoplastronneglectum / 36 / 14 / 11 / 3 / 1 / 1 / 1
Eremoplastronrostratum / 23 / 17 / 18 / 18 / 10 / 14 / 1
Eudiplodiniummaggii / 7 / 10 / 3 / 6 / 1 / 1 / 1
Eudiplodiniummaggii / 7 / 10 / 3 / 6 / 1 / 1 / 1
Eudiplodiniummaggii / 7 / 10 / 3 / 6 / 1 / 1 / 1
Eudiplodiniummaggii / 7 / 10 / 3 / 6 / 1 / 1 / 1
Eudiplodiniummaggii / 7 / 10 / 3 / 6 / 1 / 1 / 1
Eudiplodiniumrostratum / 23 / 17 / 18 / 18 / 10 / 14 / 1
Isotrichaintestinalis / 15 / 15 / 14 / 11 / 8 / 9 / 8
Isotrichaintestinalis / 15 / 15 / 14 / 11 / 8 / 9 / 8
Isotrichaintestinalis / 15 / 15 / 14 / 11 / 8 / 9 / 8
Isotrichaprostoma / 6 / 8 / 10 / 9 / 5 / 5 / 5
Isotrichaprostoma / 17 / 19 / 19 / 19 / 5 / 5 / 5
Isotrichaprostoma / 17 / 19 / 19 / 19 / 5 / 5 / 5
Isotrichaprostoma / 6 / 8 / 10 / 9 / 5 / 5 / 5
Isotricha sp. LDK-2011 / 6 / 8 / 10 / 9 / 5 / 5 / 5
Isotricha sp. LDK-2011 / 6 / 8 / 10 / 9 / 5 / 5 / 5
Isotricha sp. LDK-2011 / 6 / 8 / 10 / 9 / 5 / 5 / 5
Macropodiniumennuensis / 37 / 35 / 30 / 20 / 11 / 12 / 10
Macropodiniumyalabense / 38 / 42 / 31 / 20 / 11 / 12 / 10
Metadinium medium / 11 / 13 / 3 / 6 / 1 / 1 / 1
Metadinium medium / 11 / 13 / 3 / 6 / 1 / 1 / 1
Metadinium medium / 11 / 13 / 3 / 6 / 1 / 1 / 1
Metadinium medium / 11 / 13 / 3 / 6 / 1 / 1 / 1
Metadiniumminorum / 16 / 21 / 16 / 6 / 1 / 1 / 1
Metadiniumminorum / 16 / 21 / 16 / 6 / 1 / 1 / 1
Epidiniumcaudatum / 14 / 5 / 5 / 4 / 4 / 4 / 1
Epidiniumecaudatumcaudatum / 14 / 5 / 5 / 4 / 4 / 4 / 1
Epidiniumecaudatum fasciculus / 14 / 5 / 5 / 4 / 4 / 4 / 1
Epidinium sp. LDK-2011 / 10 / 5 / 5 / 4 / 4 / 4 / 1
Epidinium sp. LDK-2011 / 10 / 5 / 5 / 4 / 4 / 4 / 1
Epidinium sp. LDK-2011 / 10 / 5 / 5 / 4 / 4 / 4 / 1
Epidinium sp. LDK-2011 / 10 / 5 / 5 / 4 / 4 / 4 / 1
Epidinium sp. LDK-2011 / 10 / 5 / 5 / 4 / 4 / 4 / 1
Ophryoscolexcaudatus / 12 / 12 / 13 / 4 / 4 / 4 / 1
Ophryoscolexpurkynjei / 12 / 12 / 13 / 4 / 4 / 4 / 1
Ophryoscolex sp. LDK-2011 / 12 / 12 / 13 / 4 / 4 / 4 / 1
Ophryoscolex sp. LDK-2011 / 12 / 12 / 13 / 4 / 4 / 4 / 1
Ostracodinium sp. LDK-2011 / 18 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodinium sp. LDK-2011 / 18 / 1 / 1 / 1 / 1 / 1 / 1
Paraisotrichacolpoidea / 39 / 41 / 33 / 25 / 18 / 17 / 15
Polydiniellamysorea / 40 / 40 / 34 / 17 / 16 / 8 / 4
Ostracodiniumclipeolum / 2 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodiniumdentatum / 41 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodiniumgracile / 2 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodiniumgracile / 2 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodinium sp. LDK-2011 / 2 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodinium sp. LDK-2011 / 2 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodinium sp. LDK-2011 / 2 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodinium sp. LDK-2011 / 2 / 1 / 1 / 1 / 1 / 1 / 1
Ostracodiniumtrivesiculatum / 2 / 1 / 1 / 1 / 1 / 1 / 1
Polyplastronmultivesiculatum / 1 / 6 / 6 / 1 / 1 / 1 / 1
Polyplastronmultivesiculatum / 1 / 6 / 6 / 1 / 1 / 1 / 1
Polyplastronmultivesiculatum / 1 / 6 / 6 / 1 / 1 / 1 / 1
Polyplastronmultivesiculatum / 1 / 6 / 6 / 1 / 1 / 1 / 1
Polyplastron sp. LDK-2011 / 1 / 6 / 6 / 1 / 1 / 1 / 1
Polyplastron sp. LDK-2011 / 1 / 6 / 6 / 1 / 1 / 1 / 1
Polyplastron sp. LDK-2011 / 1 / 6 / 6 / 1 / 1 / 1 / 1
Polyplastron sp. LDK-2011 / 1 / 6 / 6 / 1 / 1 / 1 / 1
Raabenabella / 42 / 39 / 37 / 28 / 20 / 16 / 16
Spirodiniumequi / 22 / 23 / 25 / 12 / 13 / 11 / 4
Spirodiniumequi / 22 / 23 / 25 / 12 / 13 / 11 / 4
Triadiniumcaudatum / 44 / 25 / 36 / 22 / 15 / 8 / 4
Triadiniumcaudatum / 43 / 37 / 28 / 26 / 21 / 19 / 17
Trimyemacompressum / 26 / 24 / 20 / 15 / 17 / 15 / 9
Trimyemacompressum / 26 / 24 / 20 / 15 / 17 / 15 / 9
Trimyemaminutum / 61 / 43 / 38 / 35 / 27 / 25 / 22
Trimyemasp.koreanum / 56 / 48 / 43 / 36 / 28 / 28 / 24
Tripalmariadogieli / 45 / 34 / 35 / 23 / 22 / 8 / 4
Triplumariaselenica / 46 / 33 / 32 / 17 / 16 / 8 / 4
Troglodytellaabrassarti / 5 / 3 / 7 / 2 / 3 / 3 / 3
Troglodytellaabrassarti / 5 / 3 / 7 / 2 / 3 / 3 / 3
Troglodytellaabrassarti / 5 / 3 / 7 / 2 / 3 / 3 / 3
Troglodytellaabrassarti / 5 / 3 / 7 / 2 / 3 / 3 / 3
Troglodytellaabrassarti / 5 / 3 / 7 / 2 / 3 / 3 / 3
Troglodytellaabrassarti / 25 / 3 / 7 / 2 / 3 / 3 / 3
Troglodytellaabrassarti / 25 / 3 / 7 / 2 / 3 / 3 / 3
Troglodytellaabrassarti / 5 / 3 / 7 / 2 / 3 / 3 / 3