BTEC 3301

Sample

Chapter 6 & 7

Marks: 50 points Time: lecture time

Section A

Question 1: Multiple choice ( 2 points each= 30):

1. Plant Biotechnology chiefly involves the introduction of------genes into economically important plant species

A. more colorful

B. unproductive

C. more resistant to pests

D. foreign

2. Protoplasts are capable of fusing; forms somatic hybrids of even genetically incompatible plants.

A. True B. False

3. PEG used in Protoplast fusion, is polyethylene Glycol.

A. True B.False

4. The BT gene produced by Agrobacterium tumefaciens, produces an insecticidal protein that kills insects and their larvae .

A. True B. False

5. Gene gun mechanism are typically used to shoot DNA into the nucleus and chloroplast of plant cells.

A. True B. False

6. A transgenic bull carries a human gene for Lactoferrin.

A. True B. False

7. When cloning embryos, the DNA of the egg is gently sucked out in a process called enucleation

A. True B. False

8. ------and U.S. scientists have genetically engineered a bovine embryo that is resistant to the deadly mad cow disease and they plan to breed several of the cows to use them to make medicines to treat human diseases.

A. Indian

B. Japanese

C. Korean

D. Canadian

9. The Harvard mouse, carries a gene that promotes the development of various human cancers

A. True B. False

10. Gene gun mechanism are typically used to shoot DNA into the nucleus and chloroplast of plant cells.

A. True B. False

11.

12.

13.

14.

15.

Section B

Question 1 sample (5 points for this type)

List 5 techniques which are used by biotechnologist to insert a foreign gene to create a GM animal/crop

Question 2 sample (short answers) (5 points for this type)

Describe the antisense mechanism

Question 2 samples ( 10 points for this type)

With well labeled diagrams illustrate the process of protoplast fusion and regeneration of hybrid plants.

Using well illustrated labeled diagrams, illustrate the process of regeneration of plants from leaf discs via Agrobacterium.

Using well labeled diagrams, illustrate the process of cloning a cow.