Biotage Procedures

BIOTAGE PROCEDURES

sTarting information

You need information about:

·  Column

·  Flow Rate

·  UV detection

·  Solvent and Gradient

1) Start Up system

Click on “SPX” in the touch screen for start the software

Click on “Chemistry”

2) Method editor

If you use a method:

Select a “User”

Select a “Method”

Select “Sample name” : give a name

If you need a new method Select “Wizard” or “New”

- Separation :

Weak solvent: Select a solvent (if you don’t find your solvent click “Show all”) A

Strong solvent: Select a solvent B

Cartridge Type: Select (See manual page 5)

Flow rate: Select (See manual page 5)

- UV detector

Select collection wavelength (nm)

Select monitor wavelength (nm)

- Collection Mode

Select a collection mode (See manual page 7)

- Rack allocation

Rack type: select a rack (See manual page 9)

Dispense order

Max flow volume (check your tubes!)

- Edit gradient

(The gradient table is based on solvent B)

If you wish “initial waste” click and enter the value for Initial waste (ml)

If you wish to equilibrate click “Equilibration time”:

Start: the percentage of strong solvent B at the start of the equilibration

End : the percentage of strong solvent B at the end of the equilibration

Length : enter the equilibration volume (CV column volume or ml)

Click “Add After”:

Start: the percentage of strong solvent B at the start of gradient

End : the percentage of strong solvent B at the end of gradient

Length : enter the volume (CV column volume or ml)

If you wish add more step

3) Run a purification

Click “Status”: click “lamp ON” (wait for 10 min)

Connect the bottles of solvent to the system

Click “Solvent”:

Select “Prime”

Select “Trough column”

Select first solvent concentration 100%

Select a volume (es. 60 ml)

Select flow rate (es. 10 ml/min)

Click “Prime”

Repeat the operation for the second solvent

Click “Finish”

Click the GREEN ARROW in the method Editor

Install the column on the system

Select “Equilibration Time” : wait the finish of equilibration

Select “Load sample” : load the sample in the column

Select “Run Gradient” : the purification starts