Molecular Breeding

Cherry Leaf Spot Resistance in Cherry (Prunus) is Associated with a Linkage Group 4 Quantitative Trait Locus Inherited from P. canescens

Travis Stegmeir, Mirko Schuster, Audrey Sebolt, Umesh Rosyara, George W. Sundin and Amy Iezzoni*

Corresponding author:

Amy Iezzoni e-mail: telephone: 517-355-5191 fax: 517-353-0890

Supplementary Table S1 Sweet cherry and P. canescens plant materials used in this study and cherry leaf spot disease rating scores for 2008 to 2011

Disease Score
Genotype / Parent 1 / Parent 2 / Species / 2008 / 2009 / 2010 / 2011 / Mean / S-genotype
F5-18-167 / M30 / GerP-cana / Sweet / 1 / 1 / 2 / 2 / 1.5 / S4Scan1
Namati / - / - / Sweet / 2 / 2 / 3 / 2 / 2.3 / S1S4
GerP-can / - / - / P.canescens / - / - / - / - / - / Scan1Scan2
704010-003 / Namati / F5-18-167 / Sweet / 5 / 5 / 4 / 4 / 4.5 / S1Scan1
704010-004 / Namati / F5-18-167 / Sweet / 2 / 1 / 2 / 2 / 1.8 / S4Scan1
704010-008 / Namati / F5-18-167 / Sweet / 4 / 2 / 4 / 4 / 3.5 / S4Scan1
704010-009 / Namati / F5-18-167 / Sweet / 3 / 5 / 4 / 4 / 4 / S1Scan1
704010-010 / Namati / F5-18-167 / Sweet / 4 / 5 / 4 / 4 / 4.3 / S1Scan1
704010-015 / Namati / F5-18-167 / Sweet / 2 / 2 / 2 / 2 / 2 / S1Scan1
704010-019 / Namati / F5-18-167 / Sweet / 4 / 4 / 4 / 4 / 4 / S1Scan1
704010-022 / Namati / F5-18-167 / Sweet / 3 / 4 / 4 / 4 / 3.8 / S4Scan1
704010-025 / Namati / F5-18-167 / Sweet / 2 / 1 / 1 / 1 / 1.3 / S1Scan1
704010-028 / Namati / F5-18-167 / Sweet / 4 / 5 / 5 / 5 / 4.8 / S1Scan1
704010-029 / Namati / F5-18-167 / Sweet / 4 / 4 / 2 / 4 / 3.5 / S4Scan1
704010-034 / Namati / F5-18-167 / Sweet / 4 / 5 / 5 / 4 / 4.5 / S1Scan1
704010-037 / Namati / F5-18-167 / Sweet / 4 / 5 / 5 / 5 / 4.8 / S1Scan1
704010-050 / Namati / F5-18-167 / Sweet / - / 5 / 5 / 4 / 4.7 / S4Scan1
704010-061 / Namati / F5-18-167 / Sweet / 2 / 2 / 2 / 2 / 2 / S1Scan1
704010-062 / Namati / F5-18-167 / Sweet / 2 / 2 / 2 / 2 / 2 / S1Scan1
704010-066 / Namati / F5-18-167 / Sweet / 2 / 1 / 2 / 2 / 1.8 / S4Scan1
704010-069 / Namati / F5-18-167 / Sweet / 3 / 4 / 4 / 4 / 3.8 / S4Scan1
704010-071 / Namati / F5-18-167 / Sweet / 2 / 2 / 2 / 4 / 2.5 / S4Scan1
704010-072 / Namati / F5-18-167 / Sweet / 2 / 2 / 2 / 2 / 2 / S1Scan1
704010-074 / Namati / F5-18-167 / Sweet / 4 / 3 / - / - / 3.5 / S4Scan1
704010-078 / Namati / F5-18-167 / Sweet / 4 / 5 / 5 / 5 / 4.8 / S1Scan1
704010-079 / Namati / F5-18-167 / Sweet / 4 / 3 / 2 / 4 / 3.3 / S4Scan1
704010-083 / Namati / F5-18-167 / Sweet / 2 / 3 / 5 / - / 3.3 / S4Scan1
704010-084 / Namati / F5-18-167 / Sweet / 4 / 5 / 2 / 4 / 3.8 / S4Scan1
704010-085 / Namati / F5-18-167 / Sweet / 2 / 1 / 1 / 2 / 1.5 / S4Scan1
704010-086 / Namati / F5-18-167 / Sweet / 3 / 1 / 2 / 4 / 2.5 / S1Scan1
704010-087 / Namati / F5-18-167 / Sweet / 2 / 2 / 2 / 2 / 2 / S4Scan1
704010-093 / Namati / F5-18-167 / Sweet / 2 / 1 / 2 / 2 / 1.8 / S1Scan1
704010-099 / Namati / F5-18-167 / Sweet / - / 4 / 5 / - / 4.5 / S1Scan1
704010-125 / Namati / F5-18-167 / Sweet / 5 / 5 / 5 / 4 / 4.8 / -b
705012-005 / Namati / F5-18-167 / Sweet / 5 / 5 / 5 / 4 / 4.8 / -b
705012-020 / Namati / F5-18-167 / Sweet / 4 / 4 / 2 / 4 / 3.5 / S4Scan1
705012-025 / Namati / F5-18-167 / Sweet / 5 / 5 / 5 / 5 / 5 / S1Scan1
704010-005 / Namati / o.p.c / Sweet / 3 / 2 / 2 / 3 / 2.5 / S3S4
704010-007 / Namati / o.p. / Sweet / 4 / 3 / 4 / 4 / 3.8 / S2S4
704010-012 / Namati / o.p. / Sweet / 4 / 2 / 2 / 2 / 2.5 / S1S3
704010-013 / Namati / o.p. / Sweet / 4 / 4 / 4 / 4 / 4 / S1S3
704010-014 / Namati / o.p. / Sweet / 2 / 1 / 2 / 2 / 1.8 / S4S9
704010-016 / Namati / o.p. / Sweet / 4 / 5 / 4 / 4 / 4.3 / S1S3
704010-017 / Namati / o.p. / Sweet / 4 / 3 / 2 / 3 / 3 / S1S2
704010-020 / Namati / o.p. / Sweet / 2 / 1 / 2 / 3 / 2 / S3S4
704010-024 / Namati / o.p. / Sweet / 3 / 1 / 2 / 2 / 2 / S1S6
704010-026 / Namati / o.p. / Sweet / 3 / 2 / 2 / 4 / 2.8 / S1S2
704010-030 / Namati / o.p. / Sweet / 3 / 2 / 1 / 2 / 2 / S2S4
704010-032 / Namati / o.p. / Sweet / 3 / 1 / 3 / 2 / 2.3 / S1S2
704010-033 / Namati / o.p. / Sweet / 3 / 2 / 2 / 3 / 2.5 / S1S9
704010-035 / Namati / o.p. / Sweet / 3 / 2 / 2 / 4 / 2.8 / S1S2
704010-036 / Namati / o.p. / Sweet / 4 / 2 / 3 / 4 / 3.3 / S1S2
704010-039 / Namati / o.p. / Sweet / 5 / 5 / 5 / 4 / 4.8 / S1S6
704010-040 / Namati / o.p. / Sweet / 4 / 2 / 2 / 4 / 3 / S1S3
704010-043 / Namati / o.p. / Sweet / 2 / 2 / 2 / 2 / 2 / S2S4
704010-044 / Namati / o.p. / Sweet / 2 / 2 / 2 / 3 / 2.3 / S2S4
704010-045 / Namati / o.p. / Sweet / 2 / 1 / 2 / 2 / 1.8 / S1S3
704010-047 / Namati / o.p. / Sweet / 4 / 1 / 3 / 4 / 3 / S1S4
704010-051 / Namati / o.p. / Sweet / 4 / 4 / 4 / 4 / 4 / S4S5
704010-053 / Namati / o.p. / Sweet / 2 / 2 / 2 / 2 / 2 / S4S9
704010-054 / Namati / o.p. / Sweet / 3 / 2 / 2 / 3 / 2.5 / S2S4
704010-057 / Namati / o.p. / Sweet / 5 / 5 / 5 / 4 / 4.8 / S1S2
704010-060 / Namati / o.p. / Sweet / 3 / 2 / 2 / 2 / 2.3 / S1S3
704010-063 / Namati / o.p. / Sweet / 4 / 2 / 2 / 3 / 2.8 / S1S9
704010-064 / Namati / o.p. / Sweet / 4 / 4 / 2 / 3 / 3.3 / S1S3
704010-068 / Namati / o.p. / Sweet / 2 / 2 / 2 / 3 / 2.3 / S1S3
704010-077 / Namati / o.p. / Sweet / 2 / 1 / 2 / 2 / 1.8 / S1S9
704010-080 / Namati / o.p. / Sweet / 4 / 3 / 5 / 4 / 4 / S2S4
704010-081 / Namati / o.p. / Sweet / 3 / 1 / 2 / 3 / 2.3 / S1S3
704010-082 / Namati / o.p. / Sweet / 4 / 3 / 3 / 4 / 3.5 / S3S4
704010-091 / Namati / o.p. / Sweet / 3 / 2 / 2 / 2 / 2.3 / S3S4
704010-092 / Namati / o.p. / Sweet / 2 / 2 / 2 / 3 / 2.3 / S1S3
704010-097 / Namati / o.p. / Sweet / 3 / 2 / 2 / 3 / 2.5 / S2S4
704010-110 / Namati / o.p. / Sweet / 5 / 4 / 5 / 4 / 4.5 / S3S4
705012-002 / Namati / o.p. / Sweet / 4 / 2 / 2 / 2 / 2.5 / -
a P. canescens from Germany
b SNP markers confirmed P. canescens ancestry (data not shown)
c Open-pollinated

Supplementary Table S2 Sour cherry plant materials used in this study and cherry leaf spot disease rating scores for 2010 and 2011

Disease Score
Genotype / Parent 1 / Parent 2 / Species / 2010 / 2011 / Mean
148-1 / P.canescens / Scha / Sour / 2 / Rb / R
23-23-13 / 148-1 / o.p.c / Sour / 2 / R / R
Montmorency / - / - / Sour / - / Sd / S
UFe / - / - / Sour / - / S / S
24-32-17 / Montmorency / 23-23-13 / Sour / 2 / R / R
24-32-18 / Montmorency / 23-23-13 / Sour / 2 / R / R
24-32-20 / Montmorency / 23-23-13 / Sour / 2 / R / R
24-32-21 / Montmorency / 23-23-13 / Sour / 2 / R / R
24-32-23 / Montmorency / 23-23-13 / Sour / 3 / S / S
24-32-24 / Montmorency / 23-23-13 / Sour / 5 / S / S
24-32-25 / Montmorency / 23-23-13 / Sour / 3 / S / S
24-32-26 / Montmorency / 23-23-13 / Sour / 5 / S / S
24-32-27 / Montmorency / 23-23-13 / Sour / 2 / R / R
24-32-37 / UF / 23-23-13 / Sour / 2 / R / R
24-32-39 / UF / 23-23-13 / Sour / 3 / S / S
24-32-40 / UF / 23-23-13 / Sour / 5 / S / S
24-32-41 / UF / 23-23-13 / Sour / 2 / R / R
24-32-43 / UF / 23-23-13 / Sour / 2 / R / R
24-32-44 / UF / 23-23-13 / Sour / 4 / S / S
a Schattenmorelle
b Resistant
c Open Pollinated
d Susceptible
e Újfehértói Fürtös