ESACT-UK ANNUAL MEETING SCIENTIFIC PROGRAMME
Thursday 6th January 2005
SESSION 1 - Kindly sponsored by JRH Biosciences
Chair: Chris Mannix
13.00 Professor Ian Jones University of Reading
Recombinant baculoviruses - more and faster
13.45 Dr Kevin Richards Oxford Brookes University
Production of multiple recombinant baculoviruses using robotic systems
14.00 Shanmugapriya Santhalingam University of Birmingham
Analysis of mammalian cell culture processes at the single cell level
14.15 Leanna Jones LONZA Biologics Plc
Rapid At-Line Antibody Titre Determination Using the MININEPH™ Endpoint Nephelometer
14.30 – 15.15 Tea/ Coffee: Viewing of posters and exhibition
SESSION 2
Chair: Julian Hanak
15.15 Professor Beverly Emerson The Salk Institute, La Jolla, California
Specificity of Transcriptional Regulation through Chromatin
16.00 Gary Khoo University of Birmingham
Functional genomic analysis of genetically modified mouse NS0 cell lines in bioreactor system
16.15 Dr Chris Smith University of Cambridge
From genome to proteome: alternative splicing as a generator of isoform diversity and substrates for degradation
17.00 – 17.30 Annual General Meeting (all members are requested to attend)
18.30 – 19.15 Wine reception with exhibitors and posters
19.15 Annual Society Dinner
Sponsored by BioReliance and Invitrogen
Friday 7th January 2005
SESSION 3
Chair: Alison Stacey
09.00 Professor Jo Milner University of York
RNA interference, viruses, and cancer
09.45 Dr Cathy Hogg Cobra BioManufacturing plc
Production and Purification of adenoviral vectors for gene therapy
10.00 Dr Liz Kennings Cobra BioManufacturing plc
Critical Bio-Assays for Clinical Dosing
10.15 – 11.00 Tea/ Coffee: Viewing of posters and exhibition
SESSION 4
Chair: Tracey Zecchini
11.00 Dr John Bonham-Carter Magellan Instruments
Developments in cell culture perfusion processes and their scale-up to manufacturing
11.15 Dr Nick Hutchinson University College London
Shear Stress Analysis of Mammalian Cell Suspensions for Prediction of Industrial Centrifugation and Its Verification
SESSION 4 (continued)
11.30 Dr Glyn Stacey National Institute for Biological Standards and Control
Comparative Analysis of HIV-1 Recombinant Envelope Glycoproteins from Different Culture Systems
11.45 Kelly Astley University of Birmingham
The effect of preventing cell cycle progression through the expression of p21 on the mechanical strength and cellular robustness of CHO cell lines during adaptation to suspension and serum free growth environments
12.00 Paul Clee University of Birmingham
Preventing apoptosis by cell engineering enhances the adaptation of cell lines to serum and protein-free media
12.15 Professor Alicia El Haj Keele University Medical School
Stem Cell Therapy: Challenges for growth control and delivery
13.00 – 14.00 Lunch: Viewing of posters and exhibition
SESSION 5
Chair: John Davis
14.00 Professor Louise Jones Barts and the London Hospital
Development of 3-dimensional multicellular culture models of pre-invasive breast cancer for the replacement of animal models
14.45 Professor Michael Balls FRAME
Cell Cultures as Alternatives to Animal Testing: Progressing toward Total
Replacement in the 21st Century
15.30 Close of meeting
List of Posters
Identification of a high producing GS-CHO cell line for use in a chemically defined animal component-free fermentation process
Carole Youmbi, Mark Rendall, David Mainwaring and Andy Racher
Towards stronger gene expression – a promoter’s story
James Rance, Stephan Kalwy and Robert Gay
A platform for high-throughput molecular characterisation of recombinant monoclonal antibodies produced by mammalian cells in culture
Mark J. Bailey1, Andrew D. Hooker2, Carolyn Adams3, Shuhong Zhang3, David C. James1
On the optimal ratio of heavy to light chain genes for efficient antibody production by CHO cells
Stefan Schlatter1, Scott H. Stansfield1, Diane M. Dinnis1, Andrew J. Racher2, John R. Birch2, David C. James1
Comparative analysis of functional groups of proteins in GS-NS0 murine myeloma cell lines producing a recombinant monoclonal antibody at varying specific production rate
Diane M. Dinnis1, Scott H. Stansfield1 Stefan Schlatter1, C. Mark Smales2, John R. Birch3 Andrew J. Racher3 Carol T. Marshall4, David C. James1
In vitro modification of recombinant monoclonal antibody Fc N-glycans with a bisecting N-acetylglucosamine for improved biological activity
Jason Hodoniczky, Yuan Z. Zheng, David C. James
Germanium dioxide improves transient and stable monoclonal antibody production by CHO cells
Douglas J. Galbraith1, Catherine J. Brown1, Andrew R. Racher2, John R. Birch2, David C. James1
A High Throughput Baculovirus System
R. B. Hitchman2 K. Richards2 E. A. Siaterli2 C. Nixon2 H. Irving2 S. Mann2 R. D. Possee1 and L. A. King2
Using primary cortical neurons from wild type and transgenic mice as models of ageing and distorted heme metabolism.
T. Chernova, P. Nicotera and A. G. Smith
Novel Cell Culture Device, BelloCell, for Mass Production of Angiostatin-Human IgG Fusion Protein
Sing-Ying Hsieh, Ing-Kae Wang, Shyh-Yu Shaw, Lewis Ho and King-Ming Chang
A closer look under the bonnet: a rational approach to supercharge recombinant mRNA translation in mammalian cells
Stefan Schlatter, Diane M. Dinnis, Scott H. Stansfield, David C. James
Adaptation of chinese hamster ovary cells producing a recombinant monoclonal antibody to mild hypothermia: proteome analysis
Seong Beom Ahn, Scott H. Stansfield, Diane M. Dinnis, David C. James.