Supplementarytable 1. Growth inhibitory effects△of -Elemene (β-ELE)and/orMethoxymine (MX) treatment on C6 glioma cells (%) (mean ± SD, n = 4).

MX (mM） / β-ELE (mM)
0 / 0.06 / 0.12 / 0.24 / 0.48
0 / / / 0.88±1.1 / 18.05±2.3 / 66.54±2.4 / 78.95±4.0
31.25 / 1.88±2.2 / 7.14±2.5**＃＃ / 30.08±3.8*＃＃ / 68.71±2.5 / 78.01±1.3%
62.5 / 3.01±1.1 / 14.22±1.7*＃＃ / 43.48±2.3**＃＃ / 72.07±4.7 / 79.76±5.7
125 / 29.20±3.1 / 48.31±1.2＃ / 57.55±2.8**＃＃ / 79.95±4.9 / 81.22±6.7
250 / 77.32±1.7 / 68.34±3.8 / 69.52±2.6 / 78.27±5.7 / 81.20±3.2

vs. β-ELE group: *P < 0.05 or **P < 0.01；vs. MX group: ＃P < 0.05 or ＃＃P < 0.01.

△calculated by the following formula: [1-(ODdrug-ODcontro(B))/(ODcontrol(A)-ODcontrol(B))]×100%.

Supplementarytable 2. Growth inhibitory effects△of -Elemene (-ELE) and/orMethoxymine(MX) treatment on SHG44 glioblastoma cells (%) (mean ± SD, n = 4).

MX (mM) / β-ELE (mM)
0 / 0.06 / 0.12 / 0.24 / 0.48
0 / 0 / 1.17 ± 0.4 / 24.96 ± 2.1 / 56.76 ± 2.6 / 85.72 ± 2.1
31.25 / 27.46 ± 1.5 / 47.94 ± 3.5**＃＃ / 48.98 ± 5.4*＃ / 75.40 ± 7.3 / 88.41 ± 0.8
62.5 / 31.54 ± 1.7 / 52.89 ± 5.1*＃ / 56.10 ± 4.8*＃＃ / 77.99 ± 2.7 / 89.55 ±5.6
125 / 61.22 ± 3.2 / 64.71 ±3.9** / 70.18 ± 3.9** / 81.42 ± 5.4 / 88.21 ± 4.6
250 / 69.08 ±2.7 / 72.37 ± 5.4 / 83.10 ± 2.0 / 87.99 ± 4.0 / 86.57 ± 6.7

vs. β-ELE group: *P < 0.05 or **P < 0.01；vs. MX group: ＃P < 0.05 or ＃＃P < 0.01.

△calculated by the following formula: [1-(ODdrug-ODcontro(B))/(ODcontrol(A)-ODcontrol(B))]×100%.

Supplementarytable 3. Effect of treatment with -elemene (-ELE), methxyoamine(MX), or their combination on xeno-graft tumor volume (mean ± SD, n =8).

Group / Number of animal begin/end / Tumor volume (mm3) / RTV（） / T/C
（%）
Day1 / Day16
Control / 8/7 / 184.3 ± 58.4 / 5831.8 ± 1383.5 / 35.6 ± 12.1 / 100
β-ELE / 8/7 / 185.1 ± 61.8 / 3621.4 ± 1584.9** / 21.3 ± 6.8* / 59.9
MX / 8/8 / 185.2 ± 62.3 / 4670.1 ± 1875.7 / 25.8 ± 7.0 / 72.6
β-ELE +MX / 8/8 / 184.6 ± 58.2 / 3220.6 ± 956.1** / 18.3 ± 5.1**△ / 51.6
DDP / 8/8 / 185.5 ± 64.1 / 3793.0 ± 1383.2* / 22.6 ± 11.5 / 63.6

vs.control group:*P < 0.05 or **P < 0.01; vs.MX group: △P < 0.05.