Aujeszky’s disease

OIE Reference Laboratory Reports

Activities in 2011

Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / Aujeszky’s disease
(Pseudorabies)
Address of laboratory: / National Veterinary Services Laboratories
USDA, APHIS, VS
P.O. Box 844, 1920 Dayton Avenue
Ames, Iowa 50010 USA
Tel.: / 1-515-337-7551
Fax: / 1-515-337-7348
e-mail address: /
website: / http://www.aphis.usda.gov/animal_health/lab_info_services/
Name (including Title and Position) of Head of Laboratory (Responsible Official): / Dr Elizabeth A. Lautner
Director, National Veterinary Services Laboratories
Name(including Title and Position) of OIE Reference Expert: / Dr Sabrina Swenson
Head, Bovine, Porcine, and Aquaculture Viruses Section
Name (including Title and Position) of writer of this report
(if different from above):


Part I: Summary of general activities related to the disease

1. Test(s) in use/or available for the specified disease/topic at your laboratory

(*) Plum Island, New York Location

Test / For / Specificity / Total
ELISA gB / Antibody / Group / 358
ELISA gI / Antibody / Group / 113
LAT / Antibody / Group / 5
VN / Antibody / Group / 8

PCR

/ Nucleic acid / Group / 1

VI

/ Virus / Group / 12

2. Production and distribution of diagnostic reagents

Type of reagent / Amount supplied nationally
(including for own use) / Amount supplied to other countries
gB ELISA proficiency panel / 87
Latex agglutination proficiency panel / 48
gI ELISA proficiency panel / 90

VN proficiency panel

/ 8

Antiserum

/ 19 vials – 2ml/vial / 8 vials – 2ml/vial

FA conjugate

/ 7 vials – 1ml/vial / 7 vials – 1 ml/vial

Positive control sera

/ 35 vials – 2ml/vial / 10 vials – 2ml/vial

Negative control sera

/ 1 vials – 2 ml/vial

Virus

/ 15 vials – 0.6 ml/vial / 7 vials –0.6 ml/vial

Part II: Activities specifically related to the mandate
of OIE Reference Laboratories

3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines

a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results

No activity. Proficiency panels are available for comparison use.

b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results

No international activity. Within the United States serologic panels are provided to multiple state labs on a regular basis. Results of those panels are compared to NVSL results to ensure equivalent results. NVSL has utilized the reference material from an OIE PRV laboratory to confirm our lab’s ability to repeat the same results.

4. Preparation and supply of international reference standards for diagnostic tests or vaccines

NVSL has used the standard mentioned in item 3 to establish baselines for generating in-house positive sera.

5. Research and development of new procedures for diagnosis and control

NVSL completed evaluation of a PCR for virus detection. Manuscripts are in development.

6. Collection, analysis and dissemination of epizootiological data relevant to international disease control

Routine U.S. surveillance is conducted through two streams. One is through abattoirs where blood is collected at the time of slaughter. The second stream is through routine diagnostic specimens submitted to State and Federal diagnostic laboratories as well as testing pigs considered to be high risk for exposure to PRV from feral swine. Presumptive positive samples are referred to NVSL for confirmatory testing and follow-up by field staff.

7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned

The National Veterinary Services Laboratories’ Quality Management System is accredited to ISO/IEC 17025 by an independent accreditation body recognized by the International Laboratory Accreditation Cooperation. Audits and surveillance visits are an ongoing part of maintaining ISO/IEC 17025 accreditation.

Biosafety and biosecurity at the NVSL comply with the U. S. Department of Agriculture and U. S. Department of Health and Human Services Select Agent Program and as such meet or exceed guidelines established in “Biosafety in Microbiological and Biomedical Laboratories”.

8. Provision of consultant expertise to OIE or to OIE Member Countries

Consulted by a South American country about the potential to support them through provision of SOPs and as subject matter experts. No further contact by the country following their initial inquiry.

9. Provision of scientific and technical training to personnel from other OIE Member Countries

None requested.

10. Provision of diagnostic testing facilities to other OIE Member Countries

None requested.

11. Organisation of international scientific meetings on behalf of OIE or other international bodies

None.

12. Participation in international scientific collaborative studies

None.

13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)

¡  Presentations at international conferences and meetings

None.

¡  Scientific publications in peer-reviewed journals

None.

¡  Other communications

None.

______

Annual reports of OIE Reference Centres, 2011 3