Russell W. Carlson

Russell W. Carlson

A. ADMINISTRATION

B. INSTRUCTION

Courses taught

BCMB 3100 - 110 students - 3 contact hours per week for 12 weeks (Fall Semester 2002)

BCBM 8020 – 7 contact hours per week (April 2002)

Undergraduate Research Students

Biochemistry 4960 Matthew Hilley (spring 2002)

Biochemistry 4960 Russ Ayers (spring 2002)

Biochemistry 4960 Kahtonna Allen (spring 2002)

Biochemistry 4960 Miranda Walker (spring 2002)

Biochemistry 4960 James Russell (spring 2002)

Biochemistry 4960 Christy Rockwell (fall 2002)

Graduate Students

Vinata Vedam Microbiology

Hye-Jung Park Biochemistry and Molecular Biology

Kahtonna Allen Biochemistry and Molecular Biology

Postdoctoral Research Associates

Biswa Choudhury

Wim D’Haeze

Anup Datta

Scott Forsberg

Brent Ridley

Research Faculty

Ben Jeyaretnam

Dissertation Committees

Cristina De Meo Chemistry (Ph.D.) – Spring 2002

Dawn Adin Microbiology (Ph.D.)

Patrick Curtis Microbiology (Ph.D.)

Katharina Lohmann Veterinary Medicine (Ph.D.)

Debatosh Majumdar Chemistry (Ph.D.)

C. RESEARCH AND OTHER CREATIVE ACTIVITY:

Publications (all refereed journals)

Dean, C.R., A. Datta, R.W. Carlson, and J.B. Goldberg. 2002. WbjA adds glucose to complete the O-antigen trisaccharide repeating unit of the lipopolysaccharide of Pseudomonas aeruginosa Serogroup O11. J.Bacteriol. 184:323-326.

Shafer, W.M., A. Datta, V.S.K. Kolli, M.M. Rahman, J.T. Balthazar, L.E. Martin, W.L. Veal, D.S. Stepehens, and R. Carlson. 2002. Phase variable changes in genes lgtA and lgtC within the lgtABCDE operon of Neisseria gonorrhoeae can modulate gonococcal susceptibility to normal human serum. J. Endotoxin Res. 8:47-58 .

Tzeng, Y.-L., A. Datta, V.S.K. Kolli, R.W. Carlson, and D.S. Stephens. 2002. Endotoxin of Neisseria meningitidis composed only of intact lipid A: Inactivation of the meningococcal 3-deoxy-d-manno-octulosonic acid (Kdo) transferase. J. Bacteriol. 184:2379-2388.

Tzeng, Y.-L., A.K. Datta, C. Strole, V.S. K. Kolli, M.R. Brick, W.P. Taylor, R.W. Carlson, R.W. Woodard and D.S. Stephens. 2002. KpsF is the arabiose 5-phosphate isomerase required for 3-deoxy-d-manno-octulosonic acid biosynthesis and for both lipooligosaccharide assembly and capsular polysaccharide expression in of Neisseria meningitides. J. Biol. Chem. 277:24103-24113.

Jeyaretnam, B., J. Glushka, V.S.K. Kolli, and R.W. Carlson. 2002. Characterization of a novel lipid-A from Rhizobium species Sin-1: A unique lipid-A structure that is devoid of phosphate and has a glycosyl backbone consisting of glucosamine and 2-aminogluconic acid. J. Biol. Chem. 277:41802-41810.

Vandenplas, M.L., R.W. Carlson, B.S. Jeyaretnam, B. McNeill, M.H. Barton, N. Norton, T.F. Murray, and J.N. Moore. 2002. Rhizobium sin-1 LPS prevents enteric LPS-induced cytokine production by mono Mac 6 cells. J. Biol. Chem.. 277:41811-41816.

Loh, J., R.W. Carlson, W.S. York, and G. Stacey. 2002. Bradyoxetin, a unique chemical signal involved in symbiotic gene regulation.” PNAS. 99:14446-14451.

Vedam, V., E.L.Kannenberg, J.G. Haynes, D.J. Sherrier, A. Datta, and R.W. Carlson. 2002. A Rhizobium leguminosarum AcpXL mutant produces lipopolysaccharide lacking 27-hydroxyoctacosanoic acid.” J. Bacteriol. 185. In press.

Collaborations

Within the CCRC

Scott Forsberg, Bradley Reuhs, and Russell W. Carlson. Structural analysis of Sinorhizobium lipopolysaccharides

John Glushka, Russell W. Carlson, and Ben Jeyaretnam. Primary structure of LPS Lipid A from Rhizobium.

Between CCRC Personnel and Investigators at the University of Georgia

Russell W. Carlson and Parastoo Azadi with Michael Adang (Department of Entomology). Structural analysis of Bacillis thuringiensis toxin receptors.

Russell W. Carlson, Geert-Jan Boons, and Benjamin Jeyaretman with James Moore and Michelle Vandenplaas (Department of Large Animal Medicine, College of Veterinary Medicine). Synthesis and evaluation of novel endotoxin antagonists.

Between CCRC Personnel and Organizations in the State of Georgia (not including University of Georgia)

Russell W. Carlson and Anup Datta with David S. Stephens (Department of Medicaline, Microbiology, and Immunology, Emory University, Atlanta). Structural characterization of Neisseria menigitidis lipooligosaccharides.

Russell W. Carlson and Anup Datta with David S. Stephens (Department of Medical Microbiology, and Immunology, Emory University, Atlanta), Structural characterization of Neisseria menigitidis capsular polysaccharides.

Russell W. Carlson and Anup Datta with David S. Stephens and William Shafer (Department of Medicine, Microbiology, and Immunology, Emory University, Atlanta). Structural characterization of Neisseria gonohoreae lipooligosaccharides.

Russell W. Carlson with David Stephens (Department of Medical Microbiology, and Immunology, Emory University, Atlanta) and Conrad Quinn (Infectious Diseases, Centers for Disease Control). Characterization of the cell surface polysaccharides from Bacillus anthracis.

Within Academia and Government

Russell W. Carlson with Elmar L. Kannenberg (Microbiology/Biotechnology, University of Tübingen, Germany). Structural characterization of an LPS epitope that is specific in Rhizobium leguminosarum bv. viciae bacteroids.

Russell W. Carlson with Dale Noel (Department of Biology, Marquette University, Milwaukee, WI). Structural and functional analysis of Rhizobium etli lipopolysaccharides in legume symbiosis.

Russell W. Carlson, John Glushka, and Parastoo Azadi with Gary Stacey (Department of Microbiology, University of Tennessee, Knoxville). Structural determination of signal molecules required for Bradyrhizobium japonicum-soybean symbiosis.

Russell W. Carlson with C. Tsai (Division of Bacterial Products, FDA Center for Biologics Evaluation and Research) and David Lin (Division of Allergenic Products and Parasitology, FDA-CBER, National Institutes of Health, Bethesda, MD). Structural analysis of non-typable Hemophilus influenzae lipooligosaccharide.

Russell W. Carlson and Anup Datta with Graham Walker (Biology Department, Massachusetts Institute of Technology). Analysis of the lipid-A from Sinorhizobium meliloti.

Russell W. Carlson and Anup Datta with Graham Walker (Biology Department, Massachusetts Institute of Technology) and Marty Roop (Department of Microbiology, East Carolina University). Analysis of the lipid-A from Brucella abortus.

Russell W. Carlson, Anup Datta and Mu-Yun Gao, with Jos Vanderley (Biology Department, Lueven University, Belgium). Analysis of the LPS from R. etli CE3 mutants.

Russell W. Carlson, Anup Datta, with Jan Kijne (Plant Biology, Leiden University, The Netherlands). Analysis of the capsular polysaccharides from R. leguminosarum.

Invited presentations

North American Conference on Symbiotic Nirtrogen Fixation, Columbia, MO., June, 2002

Professional Meetings Attended

American Society for Microbiology, Salt Lake City, Utah, May 19-23, 2002

North American Conference on Symbiotic Nitrogen Fixation, Columbia, MO, June 3-8, 2002

International Endotoxin Society, Washington d. C., July 18-21, 2002

Abstracts

Vedam, V., E. Kannenberg, B. Ridley, and R.W. Carlson. “A study of the symbiotic and biosynthetic function of the 27-OHC28:0 fatty acid component of the lipid-A of Rhizobium leguminosarum 3841. 102nd General Meeting of the American Society for Microbiology, Salt Lake City, Utah, May 19-23, 2002.

Tzeng, Y., A. Datta, C.A. Strole, M.R. Birck, W.P. Taylor, R.W. Carlson, R.S. Woodard, D.S. Stephens. “KpsF is the arabinose 5-phosphate isomerase required for 3-deoxy-nd-manno-ociulosonic acid (Kdo) biosynthesis and for both lipooligosaccharide assembly and capsular polysacchardie expression in Neisseria meningitidis.” 102nd General Meeting of the American Society for Microbiology, Salt Lake City, Utah, May 19-23, 2002.

Datta, A., T.-L. Tzeng, V.K. Kolli, C.Strole, M.R. Birck, W.P. Taylor, R.W. Woodard, R.W. Carlson, D.S. Stephens. “Viable Neisseria meningitidis mutants that express only lipid A.” 7th Conference of the International Endotoxin Society, Washington, D. C., July 18-21, 2002.

Jeyaretnam, B., J. Glushka, R.W. Carlson. “Characterization of novel rhizobial lipid A structures.” 7th Conference of the International Endotoxin Society, Washington, D. C., July 18-21, 2002.

Zughaier, S.M., S.M. Zimmer, A. Data, Y.-L. Tzeng, R.W. Carlson, D.S. Stephens. “LPS binding protein (LBP) can attenuate early signal responses to cell-bound LPS.” 7th Conference of the International Endotoxin Society, Washington, D. C., July 18-21, 2002.

Grants held

Active:

Title: Rhizobial Lipopolysaccharides Essential for Infection

Agency: NIH/NIGMS

Grant Number: 2RO1GM039583-15

Project Period: 06/01/88 - 03/31/06

Current Year Award: $306,392

Role: Principal Investigator

Title: Meningococcal Lipooligosaccharide & mucosal pathogenesis

Agency: NIH/Emory University subcontract to UGA

Grant Number: 5RO1AI33517-09

Project Period: 01/01/97 - 12/31/02

Current Year Award: no-cost extension

Role: Co-Principal Investigator

Title: Sinorhizobium Lipopolysaccharide Structures Essential for Symbiotic Infection

Agency: USDA

Grant Number: 99-35305-8143

Project Period: 09/01/99 - 08/31/03

Current Year Award: $70,000

Role: Co-Principal Investigator

Title: Synthesis and evaluation of novel endotoxin antagonists

Agency: NIH/NIGMS

Grant Number: 5 RO1 GM61761-03

Project Period: 07/01/00-06/30/05

Current Year Award: $289,600

Role: Co-Principal Investigator

Title: Synthesis of P. aeruginosa LPS and its role in infection

Agency: NIH/NIAID/Univ of Virginia subcontract to UGA $23,049 Carlson’s annual direct costs

Grant Number: 1RO1AI50230-01

Project Period: 09/17/01-07/31/06

Current Year Award: $33,375

Role: Co-Investigator

Pending:

Title: Meningococcal Lipooligosaccharide & mucosal pathogenesis

Agency: NIH/Emory University subcontract to UGA

Grant Number: 5RO1AI33517

Project Period: 01/01/03 - 12/31/07 (Awarded)

Total Funds Requested: $473,017

Role: Co-Principal Investigator

Title: Bacillus Anthacis Cell Surface Carbohydrates

Agency: NIH

Grant Number: unknown

Project Period: 07/01/03 – 06/30/05

Total Funds Requested: $588,800

Role: Principal Investigator

D. PROFESSIONAL ACTIVITIES

Positions held in professional organizations

Member, International Endotoxin Society Executive Council

Member, Sigma Xi

Member, American Society for Microbiology

Member, American Society for Plant Physiology

Member, American Chemical Society

Reviews for grants

Biotechnology and Biological Sciences Research Council Research Grant

National Institute of Health, Division of Intramural Research

U. S. Department of Agriculture, Plant Biochemistry

U.S. Department of Veterans Affairs

The Wellcome Trust

Reviews for journals

Applied and Environmental Microbiology (19) –Editorial Board–Jan. 2002-Dec. 2004

Molecular Plant-Microbe Interactions

Molecular Microbiology

Phytochemistry

European Journal of Biochemistry

Carbohydrate Research (2)

Molecular & General Genomics

Canadian Journal of Chemistry

BioTechniques

Journal of Bacteriology

E. UNIVERSITY GOVERNANCE

F. OTHER CONTRIBUTIONS

Executive Technical Director of the analytical services at the CCRC.

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