Electronic Supplementary Materials (ESM) s1

Electronic supplementary materials (ESM)

ESM1: List of nematode species identified in the study site with their respective functional group and c-p values. (Based on Yeates et al. 1993 and Bongers and Bongers, 1998):

Nematode species / Trophic group / c-p value
Aphelenchoides spp. / fungal feeder / 2
Aphelenchus avenae / fungal feeder / 2
Cephalenchus hexalineatus / plant feeder / 2
Cephalenchus leptus / plant feeder / 2
Criconema sphagni / plant feeder / 3
Ogma octangulare / plant feeder / 2
Ditylenchus anchilisposomus / fungal feeder / 2
Ditylenchus spp. / fungal feeder / 2
Pseudhalenchus minutus / fungal feeder / 2
Filenchus misellus / plant feeder / 2
Filenchus discrepans / plant feeder / 2
Filenchus vulgaris / plant feeder / 2
Filenchus spp. / plant feeder / 2
Lelenchus leptosoma / plant feeder / 2
Basiria spp. / fungal feeder / 2
Ogma menzeli / plant feeder / 3
Ogma spp. / plant feeder / 3
Paratylenchus straeleni / plant feeder / 2
Rhabditida spp. / bacterial feeder / 1
Araeolaimida spp. / bacterial feeder / 2
Enoplida spp. / bacterial feeder / 3
Alaimina spp. / bacterial feeder / 4
Dorylaimida spp. / omnivore / 4
Mononchida spp. / predator / 4
other Tylenchida spp. / fungal feeder / 2

ESM2: List of plant species identified in the study area and their respective functional groups.

Plant species (with abbreviations) / Functional group
Naturally occuring herbaceous and shrub plants
Actaea rubra (Act rub) / Forb
Amelanchier spp. (Ame spp) / Forb
Anemone quinquefolia (Ane qui) / Forb
Apocynum androsaemifolium (Apo and) / Forb
Aquiligia canadensis (Aqu can) / Forb
Aralia nudicaulis (Ara nud) / Forb
Populus tremuloides (Pop tre) / Forb
Aster cordifolius (Ast cor) / Forb
Aster novae-angliae (Ast nov) / Forb
Aster umbellatus (Ast umb) / Forb
Betula papyrifera (Bet pap) / Forb
Bromus ciliatus (Bro cil) / Forb
Populus grandidentata (Pop gra) / Forb
Calamagrostis canadensis (Cal can) / Forb
Calystegia sepium (Cal sep) / Forb
Carex spp. (Car spp) / Forb
Cirsium spp. (Cir spp) / Forb
Clintonia borealis (Cli bor) / Forb
Cornus alternifolia (Cor alt) / Forb
Cornus canadensis (Cor can) / Forb
Corylus cornuta (Cor cor) / Forb
Cornus rugosa (Cor rug) / Forb
Diervilla lonicera (Die lon) / Forb
Epilobium ciliatum (Epi cil) / Forb
Fraxinus nigra (Fra nig) / Forb
Fraxinus pensylvanica (Fra pen) / Forb
Fragaria virginiana (Fra vir) / Forb
Galium triflorum (Gal tri) / Forb
Geum macrophyllum (Geu mac) / Forb
Hepatica americana (Hep ame) / Forb
Hieracium pratense (Hie pra) / Forb
Lathyrus venosus (Lat ven) / Forb
Linnaea borealis (Lin bor) / Forb
Aster macrophyllus (Ast mac) / Forb
Lonicera canadensis (Lon can) / Graminoid
Lonicera hirsuta (Lon hir) / Graminoid
Lycopodium spp (Lyc spp) / Graminoid
Maianthemum canadense (Mai can) / Graminoid
Acer spicatum (Ace spi) / Graminoid
Muhlenbergia spp. (Muh spp) / Graminoid
Oryzopsis asperifolia (Ory asp) / Graminoid
Osmorhiza claytonii (Osm cla) / Graminoid
Plantago major (Pla maj) / Legume
Uvularia sessilifolia (Uvu ses) / Legume
Polygonum spp. (Pol spp) / Legume
Potentilla spp. (Pot spp) / Woody Shrubs
Prenanthes alba (Pre alb) / Woody Shrubs
Prunus pensylvanica (Pru pen) / Woody Shrubs
Prunus virginiana (Pru vir) / Woody Shrubs
Pteridium aquilinum (Pte aqu) / Woody Shrubs
Pyrola spp. (Pyr spp) / Woody Shrubs
Ribes triste (Rib tri) / Woody Shrubs
Acer rubrum (Ace rub) / Woody Shrubs
Rubus idaeus (Rub ida) / Woody Shrubs
Rubus pubescens (Rub pub) / Woody Shrubs
Salix spp. (Sal spp) / Woody Shrubs
Schizachne purpurascens (Sch pur) / Woody Shrubs
Scirpus cyperinus (Sci cyp) / Woody Shrubs
Solidago altissima (Sol alt) / Woody Shrubs
Solidago gigantea (Sol gig) / Woody Shrubs
Sonchus spp. (Son spp) / Woody Shrubs
Streptopus roseus (Str ros) / Woody Shrubs
Tanacetum vulgare (Tan vul) / Woody Shrubs
Taraxacum officinale (Tar off) / Woody Shrubs
Trientalis borealis (Tri bor) / Woody Shrubs
Trifolium spp. (Tri spp) / Woody Shrubs
Vaccinium spp. (Tri spp) / Woody Shrubs
Vicia americana (Vic ame) / Woody Shrubs
Viola spp. (Vio spp) / Woody Shrubs
Planted tree seedlings
Abies balsamea (Abi bal)
Populus tremuloides (Pop tre)
Picea glauca (Pic gla)
Pinus strobus (Pin str)
Pinus banksiana (Pin ban)
Quercus rubra (Our rub)
Quercus macrocarpa (Oue mac)
Rhamus cathartica (Rha cat)
Betula payprifera (Bet pap)
Acer rubrum (Ace rub)
Acer sccharum (Ace scc)

ESM3: Mean ± standard error for nematode indices in response to temperature and canopy treatments at two experimental sites: a) Cloquet and b) Ely.

a) Cloquet

Nematode indices / Closed canopy (°C) / Open canopy (°C)
Ambient / Ambient+ 1.7 / Ambient+ 3.4 C / Ambient / Ambient+ 1.7 C / Ambient+ 3.4 C
Total density / 87.17 / ±20.38 / 45.67 / ±10.89 / 63.33 / ±17.94 / 82.67 / ±20.72 / 81.40 / ±22.36 / 70.20 / ±9.81
Fungal feeders density / 27.00 / ±5.60 / 18.67 / ±6.06 / 26.50 / ±9.69 / 39.83 / ±12.17 / 34.80 / ±9.54 / 31.60 / ±3.61
Bacterial feeders density / 11.00 / ±2.33 / 11.83 / ±3.20 / 19.00 / ±8.67 / 25.83 / ±7.60 / 28.40 / ±13.12 / 25.20 / ±5.13
Plant feeders density / 12.83 / ±6.60 / 6.83 / ±3.82 / 10.67 / ±5.33 / 4.50 / ±1.31 / 8.60 / ±2.90 / 2.80 / ±1.39
Predators density / 2.67 / ±1.22 / 0.67 / ±0.49 / 0.67 / ±0.33 / 3.33 / ±1.05 / 1.00 / ±0.63 / 0.40 / ±0.4
Omnivores density / 13.00 / ±2.56 / 7.50 / ±2.04 / 6.67 / ±3.05 / 8.33 / ±4.03 / 8.80 / ±2.39 / 11.00 / ±1.70
Total richness / 7.33 / ±0.42 / 6.00 / ±0.73 / 6.50 / ±1.25 / 8.50 / ±0.56 / 7.00 / ±0.83 / 7.60 / ±0.67
Shannon Wiener index / 0.62 / ±0.04 / 0.63 / ±0.07 / 0.60 / ±0.09 / 0.76 / ±0.03 / 0.67 / ±0.07 / 0.69 / ±0.06
Pielou Evenness / 0.73 / ±0.06 / 0.81 / ±0.06 / 0.77 / ±0.05 / 0.83 / ±0.04 / 0.80 / ±0.05 / 0.78 / ±0.03
cp1 richness / 1.0 / 1.0 / 1.0 / 1.0 / 1.0 / 1.0
cp2 richness / 4.17 / ±0.16 / 3.33 / ±0.55 / 4.00 / ±0.73 / 4.50 / ±0.88 / 4.40 / ±0.67 / 5.20 / ±0.86
cp3 richness / 0.33 / ±0.21 / 0 / 0 / 0.83 / ±0.16 / 0.40 / ±0.24 / 0.40 / ±0.24
cp4 richness / 1.67 / ±0.21 / 1.50 / ±0.34 / 1.67 / ±0.49 / 1.83 / ±0.40 / 1.40 / ±0.24 / 1.20 / ±0.20
MI / 2.29 / ±0.09 / 2.07 / ±0.16 / 1.97 / ±0.09 / 2.26 / ±0.17 / 2.04 / ±0.13 / 2.02 / ±0.05
PPI / 1.18 / ±0.12 / 0.69 / ±0.20 / 1.04 / ±0.14 / 0.72 / ±0.16 / 0.55 / ±0.23 / 0.57 / ±0.21
FF/FF+BF / 0.16 / ±0.06 / 0.23 / ±0.04 / 0.20 / ±0.08 / 0.15 / ±0.05 / 0.37 / ±0.05 / 0.30 / ±0.08
FF+BF/PF / 0.29 / ±0.08 / 3.14 / ±2.38 / 0.71 / ±0.18 / 0.88 / ±0.09 / 1.59 / ±0.41 / 2.97 / ±1.25
PR/PF / 0.39 / ±0.12 / 0.50 / ±0.15 / 0.22 / ±0.09 / 0.64 / ±0.26 / 0.48 / ±0.15 / 0.76 / ±0.21

b) Ely

Nematode indices / Closed canopy (°C) / Open canopy (°C)
Ambient / Ambient+ 1.7 C / Ambient+ 3.4 C / Ambient / Ambient+ 1.7 C / Ambient+ 3.4 C
Total density / 158.17 / ±37.76 / 134.67 / ±30.00 / 116.17 / ±26.89 / 148.50 / ±55.49 / 120.50 / ±31.94 / 152.67 / ±38.67
Fungal feeders density / 50.83 / ±11.38 / 37.50 / ±8.09 / 29.00 / ±4.32 / 73.67 / ±53.88 / 32.67 / ±9.53 / 30.17 / ±9.71
Bacterial feeders density / 19.83 / ±10.64 / 33.00 / ±10.72 / 36.17 / ±12.41 / 26.67 / ±4.71 / 57.50 / ±25.98 / 63.00 / ±32.61
Plant feeders density / 73.50 / ±31.38 / 55.67 / ±21.24 / 46.33 / ±15.16 / 8.17 / ±5.31 / 4.67 / ±1.35 / 11.00 / ±4.74
Predators density / 2.33 / ±1.30 / 0.83 / ±0.65 / 1.17 / ±0.54 / 4.83 / ±2.34 / 4.00 / ±1.54 / 3.33 / ±1.20
Omnivores density / 12.17 / ±7.14 / 8.00 / ±0.93 / 8.50 / ±4.09 / 24.33 / ±6.21 / 33.67 / ±14.84 / 44.00 / ±12.23
Total richness / 7.83 / ±1.13 / 7.00 / ±0.57 / 8.83 / ±0.79 / 7.17 / ±0.79 / 7.50 / ±1.14 / 7.33 / ±0.71
Shannon Wiener index / 0.58 / ±0.04 / 0.66 / ±0.03 / 0.73 / ±0.03 / 0.61 / ±0.07 / 0.58 / ±0.07 / 0.60 / ±0.04
Pielou Evenness / 0.67 / ±0.03 / 0.79 / ±0.039 / 0.78 / ±0.04 / 0.71 / ±0.07 / 0.69 / ±0.06 / 0.70 / ±0.04
cp1 richness / 1.0 / 1.0 / 1.0 / 1.0 / 0.83 / ±0.16 / 1.0
cp2 richness / 5.33 / ±0.71 / 4.50 / ±0.61 / 5.17 / ±0.40 / 3.33 / ±0.42 / 4.00 / ±0.81 / 3.83 / ±0.30
cp3 richness / 0.17 / ±0.16 / 0.17 / ±0.16 / 0.67 / ±0.33 / 1.00 / ±0.44 / 0.67 / ±0.33 / 0.50 / ±0.22
cp4 richness / 1.33 / ±0.49 / 1.50 / ±0.22 / 1.83 / ±0.40 / 1.83 / ±0.30 / 2.17 / ±0.30 / 1.83 / ±0.30
MI / 2.03 / ±0.16 / 1.94 / ±0.04 / 1.95 / ±0.06 / 2.37 / ±0.21 / 2.46 / ±0.30 / 2.48 / ±0.25
PPI / 1.27 / ±0.25 / 0.89 / ±0.09 / 0.82 / ±0.23 / 0.84 / ±0.20 / 0.31 / ±0.17 / 0.20 / ±0.08
FF/FF+BF / 0.30 / ±0.10 / 0.10 / ±0.04 / 0.18 / ±0.05 / 0.14 / ±0.03 / 0.29 / ±0.14 / 0.18 / ±0.05
FF+BF/PF / 0.64 / ±0.51 / 0.40 / ±0.08 / 0.69 / ±0.27 / 1.02 / ±0.46 / 8.18 / ±4.64 / 7.70 / ±5.88
PR/PF / 0.16 / ±0.10 / 0.11 / ±0.02 / 0.17 / ±0.09 / 0.74 / ±0.21 / 6.58 / ±4.27 / 3.02 / ±1.03

References

Yeates, GW, Bongers, T, De Goede, R.G, Freckman, DW, & Georgieva, SS(1993). Feeding habits in soil nematode families and genera-an outline for soil ecologists. Journal of nematology, 25(3), 315–31.

Bongers T, Bongers M (1998) Functional diversity of nematodes. Applied Soil Ecology 10:239–251