Supplemental Table 1. Primers and annealing temperatures used for probe production

N / Probe / Selected PCR product length, bp / Primers 5’-3’ / Template / Sequence accession N
1 / BACbz / 3480
1636
1899
3444
1869 / TCG ATC ATC ATC TCC TGC GAA TTT C
ATT GTC ACG GGA TCT GGT CTA AAC
CTA TGT TCT TTC CTT CTT GCG TTA T
GCG ATT TCT TCC TCA CCT ACT CC
TCT TGA AAT TTA GAC TTC GTG TGC C
GTG ATT TGT TTT AGC TTG CGT GT
ACA GGC AAG AGC ATG AAC GTG
TGC GGT TGT GCG TAT GA
GGG CAA CTT TGT TAC ACT CAC GAA C
TTC CAC GCT TCA ATT TCC CTA CCT / Genomic DNA B73 / AF448416
2 / BACwx / 2319
3679
1454 / AAA TGT GTG CGC TCC TTA GAT A
CGT CTA TGT CAC CTG CGT CTA C
AGC CAT AAG TGC CTA ATC G
CTC TAC ATT TGG CAA CCA TT
GCA TCT ACA GGG ACG CAA AGG
AAC GGC GGT GCT GAT TCA TAC / Genomic DNA B73 / AF488416
3 / rf2a / 3900
3512 / CAG CGC TAG CTC AGT TGT CGA T
TCA CCT GCC CAA CCG TCT C
GGA TCG GAG GAA CCA GTA G
CAA AGC CCA TTC TCT CTA AGT A / Genomic DNA B73 / AF215823
4 / BAC-S1 / 2811 / GGA AGC AGC AAA CCT TTC G
TTA GTG GCA GCC CAT AAT TAG C / Genomic DNA B73 / AC155534
5 / BAC S2 / 1105
2955
2482
931 / CGG AAA GGC TCG CTT ATT ATC T
CAT GGT TAT TTC CGA GCT TCT C
TGA CAG GCG CTT CAT CTT TAC C
CAA CGA CTC CTC ACC CAA ATC T
CGC CTC CAA CAT CAC GCT ATT C
ATG CAC CAC CAC AAG CCA AAG
CAG GCT GGT TCA ATG CAT TGG G
CCA TGC TGC TTG CGT TGA AGG T / BAC DNA / AC187809
6 / CentC / 540 / M13 / Clone / Centromeric repeat Clone from Dr. R.Philips
7 / gl15 / 2907 / CCC GGC GCA TAG ATA GAG A
AAT GGG TAG CAG AAT CAA CGA G / Genomic DNA B73 / AY714877
8 / mRNA / 4000 / GAA GAG CGT GTG CGT GAC
TTG GGA TAA GGG TAA GAC GGA T / Genomic DNA B73 / AY103770
9 / BAC-L1 / 2858
1188
1175
3880
1484 / TAG TCT ACG TGG CGC TTC ATC TT
TGC TTG GCG TAC AGG GTC TC
TCC AGC CCG TTC AAA CTG C
TCT CTA TGC GGA GTG CGA CTG
CCG AGC AGG ACA CCA TGG AT
CCT AGG CCA CAC GAT ACA GCA CTT
CAA GAA GCC CAT GTG GAT AAT CGT
CAC AAG GCA AAG AAT CCG GTA GG
AGG TAC ACG TCG CCG AGC TTA T
TGA ATC ATT TGC CAC TCT TTG CTT / BAC DNA / AC188012
10 / expB / 3 x 1000 / M13 / cDNA clones from E.Valdivia
11 / sus1 / 3177
3928 / GTC CAT TTG ATT TGC GTT CAC T
GGC TCA CCA TCC CCT TGT AGT
CCT TCT CTG TCA AAG TCC ATT
AGT AAG ACA CCG TAT CAA GGT CTA / L33244
12 / BAC-L2 / 978
2037
992
2666
1998 / GTC CTT GCT TGC CGC TAC A
CGA TCC GTC CAG CCC TAC TA
CCT CGC ATG ATC GTT CAA GTG G
GAA CGG CGA GTC CTG GGT G
GAA GCC GAC TCA CCT GTG C
CGC CAC CAA ATC CCT ACC TC
CCA CCG GCA TCT CCA A
CAC GCA AGC ATC AAC CTA GAG
GTT GGG TGA TTA CGA CGG AGT
TGG CAC CTA CAA TAT ATC ACA GCA / BAC DNA / AC187139
13 / BAC-L3 / 1239
1187
1811
1962 / GGA CCC GCA CTT CTA CGA CA
AGC ACG CTG ACA TGG TTC TAG AC
GTA GAG GCG TGT GCG TGT G
TCA CTC ATT CCC ACG CTG TAT AA
TGT TCC GGT AGA CCA TCC CAG
TGC TCT CCT GCC CGA ACT CT
CAG GAG AAC AGA AAG CCC AG
GGC GTC TTC CCA CAT ATG AGT A / BAC DNA / AC155405
14 / BAC-L4 / 2374
2472
2364
2552
2869
3570
1504
3089 / GGC CCT CAT GAA GAT ATC AGT
ATT CGC AAC ATA ACA GTA GGT CA
CGA CGA GTT TGA GAC AGA CCA
CTA GAG CAA AGC CGA ACT TCA GA
AAA AGC AGA AAA GCG TAT CAT GGA
GCT GAG CCG CGA TAG GAG AG
GCC GTG ATT CGA TGT TTA CAG T
CAC CTT GAG TTG CCC ACA G
AAG CTC GCT GCC ATC TCT AGT GT
TCG TTG CCC TCA TCT GGA ATC
CGC AGA GGA AGA TAA ACG AGA TG
AGT TTC CAT TCG CTG TCG C
CCC TCG TAC AGA CCC TCG C
TAG ACA TGG GAA ATT GGG AAT GTG
GCC TCC CTC TCC TCT TCA C
AAG GCA ACT ATG GAA TTC TAT GG / BAC DNA / AC187803
15 / BAC-L5 / 3314
3948
3475
1377 / ATT GCA GAT GCC CTT GTA AGT T
TTG GCA CAA ATC CGA GCT AT
GAT ATA GCA GGT GAC AGC GAT T
GCC GAG TAG GGT ATC AAT GT
AAC CCA AAA CCA AAC ATG AAC CTA
CAT CCG CGA GAA ACT TAA CCC T
TTA ATA TCA ACA TGG CAA ACG
AAA AAC ATT TAA AGG CAG ACA TAG / Genomic DNA B73 / AC149033