DNA primers
10x Kinase buffer:700mM Tris-HCl pH 7.6100mM MgCl250mM DTT
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of supervisor / Dr S. Minchin / Assessment Number* / 1401Signature
Assessor / Rita Godfrey / Date of Assessment / 12 / 6 / 01
Signature
NotesA School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1 / LOCATION OF THE WORK ACTIVITY / Lab G10 and G42 / PERSONS WHO MAY BE AT RISK
List names where possible / All occupants of the lab (Laura Tate, Jo Field, Dong Ling Zeng, Rita Godfrey)
3 / ACTIVITY ASSESSED / Labelling DNA primer with [32P] for footprinting
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT
max/stored / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
DNA primers / 1ml / NSH
10x Kinase buffer:
700mM Tris-HCl pH 7.6
100mM MgCl2
50mM DTT / NSH
gamma[32P] ATP / RADIO-
ACTIVE
T4 Polynucleotide kinase / 50ul / No data but probably NSH / No R no.s but avoid skin contact and inhalation as prolonged or repeated exposure may cause allergic reactions in sensitive individuals
Stratagene NucTrap Purification columns
Cat. no. 400701 / 25 columns / NSH
STE buffer: 100mM NaCl, 20mM Tris-HCl pH 7.5, 10mM EDTA / 100ml / NSH
5 / INTENDED USE**
Give brief details and attach protocol/instructions / End labelling DNA primer for use in footprinting See attached sheet .
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
There is a risk of exposure to radiation from the [32P]
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Risk of exposure to radiation is significant. Follow approved scheme 1/P. The use of Stratagene NucTrap
purification columns reduces the risk of radiation exposure when used with the push column beta shield
device. The old way with home-made mini Sephadex G50 columns increased the risk of exposure.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
Work behind a perspex screen in lab G4 whilst working with [32P]. Wear protective clothing and gloves
at all times . Monitor the work area with a Geiger counter before and after use and monitor gloves regularly
This work is covered by the University Radiation Scheme number1/P
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
University radiation safety course
10 / MONITORING
Performance of control measures,
Check work area with a Geiger counter before and after use. Check integrity of gloves. Monitor gloves regularly. Make sure Geiger counter is working properly before starting.
Personal exposure / Health Surveillance
Radiation film badge which is changed each month
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Radioactive waste is disposed of in accordance with the University Radiation Regulations.
The used columns are very “hot” and so are put in the high level radioactive waste container in lab G4
along with pipette tips used to dispense the stock [32P]ATP. All single items below 1uCi and totalling less than 10uCi are put in the low level waste bin.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
June 2006
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Spills of radioactive liquid should be mopped up with paper towels which are then bagged and monitored to estimate the amount of radioactivity on them. They can then be placed in the waste bin in G4 designated
for low level [32P] waste. The amount of radioactivity is entered on the disposal sheet.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Protective clothing and gloves must be worn at all times along with a radiation film badge. Work should take place behind a perspex screen in lab G4 (hot lab)
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
The site should be washed with Decon diluted as indicated on the container until monitoring of the site produces no counts on the Geiger counter. The paper towels used for this should be bagged and disposed of as above. When the site has been decontaminated with Decon it can be washed with water.
CONTACT PHONE
10.10.00
* Prefix T is used for Teaching Assessment Number.
** Please include amount of chemicals used and how.
***Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.