Supplementary content

Table S1. Measured concentrations of tPAH50 in spiked reference sediments at experiment termination

Sediment Mass (g) / Oil Mass (g) / Nominal loading Rate (g oil/ kg sediment wet wt.) / Measured tPAH(50)
(mg/kgdry wt.) at termination
3001 / 0.74 / 0.25 / 0.3
3000 / 1.11 / 0.37 / 0.6
3002 / 1.92 / 0.64 / 1.0
3000 / 3.72 / 1.2 / 2.6
3001 / 6.95 / 2.3 / 5.5
3000 / 12.44 / 4.2 / 11.4
3000 / 23.79 / 7.9 / 24.2

Table S2. A list of the 50 analytes used to calculate tPAH(50).

Analyte / Symbol
Naphthalene / N0
C1-Naphthalenes / N1
C2-Naphthalenes / N2
C3-Naphthalenes / N3
C4-Naphthalenes / N4
Biphenyl / B
Dibenzofuran / DF
Acenaphthylene / AY
Acenaphthene / AE
Fluorene / F0
C1 - Fluorenes / F1
C2 - Fluorenes / F2
C3 - Fluorenes / F3
Anthracene / A0
Phenanthrene / P0
C1-Phenanthrenes/Anthracenes / PA1
C2-Phenanthrenes/Anthracenes / PA2
C3-Phenanthrenes/Anthracenes / PA3
C4-Phenanthrenes/Anthracenes / PA4
Dibenzothiophene / DBT0
C1 - Dibenzothiophenes / DBT1
C2 - Dibenzothiophenes / DBT2
C3 - Dibenzothiophenes / DBT3
C4 - Dibenzothiophenes / DBT4
Benzo(b)fluorene / BF
Fluoranthene / FL0
Pyrene / PY0
C1 - Fluoranthenes/Pyrenes / FP1
C2 - Fluoranthenes/Pyrenes / FP2
C3 - Fluoranthenes/Pyrenes / FP3
C4-Fluoranthenes/Pyrenes / FP4
Naphthobenzothiophene / NBT0
C1-Naphthobenzothiophenes / NBT1
C2-Naphthobenzothiophenes / NBT2
C3-Naphthobenzothiophenes / NBT3
C4-Naphthobenzothiophenes / NBT4
Benz(a)anthracene / BA0

Table S2(continuation)

Analyte / Symbol
Chrysene+Triphenylene / C0
C1 - Chrysenes / BC1
C2 - Chrysenes / BC2
C3 - Chrysenes / BC3
C4 - Chrysenes / BC4
Benzo(b)fluoranthene / BBF
Benzo(j+k)fluoranthene / BJKF
Benzo(a)fluoranthene / BAF
Benzo(e)pyrene / BEP
Benzo(a)pyrene / BAP
Indeno(1,2,3-cd)pyrene / IND
Dibenz(a,h)anthracene / DA
Benzo(g,h,i)perylene / GHI

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Table S3. Measured concentrations of PAH50 (µg/kg dry wt.)compounds in spiked reference sediments at experiment termination.

Analyte / Control / Reference / Treat. 1 / Treat. 2 / Treat. 3 / Treat. 4 / Treat. 5 / Treat. 6 / Treat. 7
Naphthalene / 6.81 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
C1-Naphthalenes / 18.1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
C2-Naphthalenes / 43.4 / 2.68 / 3.28 / 2.46 / 3.1 / 5.43 / 10.2 / 17.6 / 38.9
C3-Naphthalenes / 30.4 / 5.61 / 7.74 / 9.19 / 15.3 / 34.5 / 68.3 / 131 / 332
C4-Naphthalenes / 22.5 / 0 / 14.8 / 21.1 / 32.1 / 86.5 / 166 / 307 / 659
Biphenyl / 5.82 / 0 / 0 / 0 / 0 / 0 / 0 / 1.98 / 2.84
Dibenzofuran / 3.86 / 0 / 0 / 0 / 0 / 0.679 / 1.36 / 1.28 / 2.25
Acenaphthylene / 1.51 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Acenaphthene / 1.07 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 2.99
Fluorene / 5.45 / 0 / 0.733 / 1.2 / 1.53 / 3.98 / 6.26 / 10.6 / 32.8
C1 - Fluorenes / 9.9 / 0 / 5.21 / 7.51 / 12.4 / 31.2 / 69.8 / 156 / 344
C2 - Fluorenes / 0 / 0 / 17.8 / 23.4 / 41 / 113 / 243 / 495 / 1150
C3 - Fluorenes / 0 / 0 / 17.7 / 36.1 / 66 / 162 / 364 / 709 / 1450
Anthracene / 4 / 0 / 0 / 0 / 0 / 2.84 / 3.78 / 6.83 / 15.6
Phenanthrene / 23.2 / 0 / 4.89 / 7.45 / 13 / 31.4 / 63.9 / 140 / 318
C1-Phenanthrenes/Anthracenes / 32.3 / 0 / 25.8 / 40.4 / 74.1 / 201 / 436 / 959 / 2070
C2-Phenanthrenes/Anthracenes / 27.6 / 0 / 49.9 / 77 / 136 / 345 / 769 / 1640 / 3460
C3-Phenanthrenes/Anthracenes / 23.7 / 0 / 38.5 / 61.7 / 111 / 271 / 600 / 1210 / 2470
C4-Phenanthrenes/Anthracenes / 4.03 / 0 / 29.6 / 56.5 / 84 / 199 / 409 / 804 / 1660
Dibenzothiophene / 2.02 / 0 / 0.756 / 1.28 / 1.75 / 3.74 / 8.82 / 20.1 / 45.5
C1 - Dibenzothiophenes / 0 / 0 / 6.59 / 9.95 / 16.3 / 39.7 / 88.2 / 197 / 411
C2 - Dibenzothiophenes / 0 / 0 / 15.1 / 25.5 / 46.1 / 107 / 244 / 539 / 1120
C3 - Dibenzothiophenes / 0 / 0 / 14 / 29.8 / 46.2 / 109 / 250 / 504 / 1070
C4 - Dibenzothiophenes / 0 / 0 / 6.93 / 10.5 / 57 / 100 / 225 / 362 / 710
Benzo(b)fluorene / 5.19 / 0 / 0 / 0.894 / 1.31 / 3.33 / 6.55 / 13.3 / 30.9
Fluoranthene / 37.9 / 0 / 1.12 / 1.39 / 1.95 / 3.76 / 6.47 / 12.8 / 25.7
Pyrene / 25.9 / 0 / 2.49 / 2.13 / 3.21 / 6.36 / 12.3 / 25.5 / 57.6
C1 - Fluoranthenes/Pyrenes / 19.9 / 0 / 5.3 / 8.33 / 12 / 27.4 / 63 / 139 / 293
C2 - Fluoranthenes/Pyrenes / 0 / 0 / 0 / 10.5 / 20.2 / 42.7 / 91.5 / 193 / 425
C3 - Fluoranthenes/Pyrenes / 0 / 0 / 0 / 15.5 / 22.1 / 46.6 / 106 / 227 / 467
C4-Fluoranthenes/Pyrenes / 0 / 0 / 0 / 18.5 / 38.6 / 62.7 / 131 / 245 / 552
Naphthobenzothiophene / 2.97 / 0 / 0 / 1.85 / 2.75 / 7.72 / 20.1 / 45.8 / 107
C1-Naphthobenzothiophenes / 0 / 0 / 0 / 10.1 / 15.9 / 36.1 / 80.4 / 181 / 450
C2-Naphthobenzothiophenes / 0 / 0 / 0 / 20.2 / 34.4 / 57.5 / 128 / 273 / 555
C3-Naphthobenzothiophenes / 0 / 0 / 0 / 18.1 / 0 / 45.8 / 94.5 / 211 / 384
C4-Naphthobenzothiophenes / 0 / 0 / 0 / 0 / 0 / 32.6 / 73.4 / 132 / 251
Benz(a)anthracene / 8.69 / 0 / 0 / 0.76 / 1.18 / 1.96 / 1.71 / 4.93 / 7.98
Chrysene+Triphenylene / 15.8 / 0 / 3.54 / 6.25 / 10.9 / 28.4 / 72.4 / 171 / 408
C1 - Chrysenes / 0 / 0 / 14.6 / 17.6 / 29.4 / 73.4 / 178 / 396 / 876
C2 - Chrysenes / 0 / 0 / 22.6 / 29.4 / 50.3 / 95.3 / 198 / 409 / 884
C3 - Chrysenes / 0 / 0 / 0 / 21.3 / 37.2 / 66.6 / 116 / 247 / 522
C4 - Chrysenes / 0 / 0 / 0 / 0 / 0 / 57.1 / 113 / 193 / 397
Benzo(b)fluoranthene / 15.8 / 0 / 0 / 1.57 / 1.96 / 4 / 8.16 / 18.7 / 41.5
Benzo(j+k)fluoranthene / 5.83 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Benzo(a)fluoranthene / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Benzo(e)pyrene / 10 / 0 / 0 / 2.05 / 3.05 / 6.28 / 14.1 / 32.9 / 72.6
Benzo(a)pyrene / 9.12 / 0 / 0 / 0 / 0 / 0 / 0 / 3.34 / 7.35

Table S3 (continuation)

Analyte / Control / Reference / Treat. 1 / Treat. 2 / Treat. 3 / Treat. 4 / Treat. 5 / Treat. 6 / Treat. 7
Indeno(1,2,3-cd)pyrene / 7.86 / 0 / 0 / 0.551 / 0 / 0 / 0 / 0 / 0
Dibenz(a,h)anthracene / 0 / 0 / 0 / 0.613 / 0 / 0 / 2.23 / 3.5 / 7.3
Benzo(g,h,i)perylene / 9.23 / 0 / 0 / 1.14 / 0 / 1.89 / 3.22 / 5.1 / 12.8
Sum TPAH / 439.86 / 8.29 / 308.979 / 609.768 / 1043.29 / 2554.469 / 5546.66 / 11394.26 / 24199.61

Table S4. Leptocheirusplumulosus28-day sediment exposure: Mean percent survival, growth rate, and percent reproductive output as offspring per surviving adult. Numbers in parenthesis represent 1 standard deviation. * Represents significant difference from the reference mean.

Treatment tPAH50 (mg/kg) / Percent survival / Growth rate (mg/d) / Offspring per surviving adult
0.008 (Reference) / 83.0 (16.8) / 0.036 (0.002) / 0.89 (0.70)
0.3 / 62.0 (19.6) / 0.037 (0.006) / 1.32 (0.69)
0.6 / 62.0 (24.1) / 0.031 (0.008) / 0.82 (1.07)
1.0 / 72.0 (17.2) / 0.031 (0.007)* / 0.57 (0.95)
2.6 / 41.0 (20.4)* / 0.027 (0.004)* / 0.16 (0.36)
5.5 / 64.0 (27.0) / 0.029 (0.006)* / 0.16 (0.23)
11.4 / 51.0 (21.6)* / 0.028 (0.007)* / 0.08 (0.14)
24.2 / 45.0 (21.5)* / 0.025 (0.007)* / 0.05 (0.10)*

Figure S1. Composition of tPAH (50) in Slick B oil (bottom) and reference sediment spiked with Slick B oil (top). Analytes used to calculate tPAH(50)are listed in Table S2.

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