Supplementary Figure Legends
Fig. S1 No effect of miR-490-3p on necrosis and apoptosis. (A-C) MiR-490-3p did not alter necrotic or apoptotic cell death in AGS cell as determined by (A) lactate dehydrogenase release assay (72 hours post-transfection), (B) annexin V staining (48 hours post-transfection) and (C) terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (72 hours post-transfection). **, p < 0.01; ***, p < 0.001, significantly different from respective control group.
Fig. S2 Expression and function of miR-490-3p. (A) Real-time PCR confirmed that the expression levels of miR-490-3p in mimics-transfected AGS cells were comparable to that of non-cancerous gastric mucosa. Total RNA was isolated at 96 hours post-transfection. (B) Lentiviral overexpression of miR-490-3p significantly inhibited the growth of MKN45 xenograft in nude mice. **, p < 0.01; ***, p < 0.001, significantly different from respective control group.
Fig. S3 Overexpression of SMARCD1 but not PCBP2 in gastric cancer cell lines. **, p < 0.01; ***, p < 0.001, significantly different from HFE-145.
Fig. S4 Effects of SMARCD1 knockdown and overexpression on oncogenic phenotypes of gastric cancer cells. (A-C) Knockdown of SMARCD1 by siRNA mimicked the effects of miR-490-3p by inhibiting (A) colony formation (2 weeks post-transfection), (B) migration (48 hours post-transfection) and (C) invasion (72 hours post-transfection) of gastric cancer cells. (D-F) Enforced expression of SMARCD1 increased (D) colony formation (2 weeks post-transfection) and (E) migration (48 hours post-transfection) of gastric cancer cells as well as (F) tumor growth of TMK-1 xenograft. *, p < 0.05; **, p < 0.01, significantly different from respective control group.
Fig. S5 Attenuation of tumor-suppressing effects of miR-490-3p by SMARCD1 overexpression. Overexpressing a SMARCD1 construct without 3'UTR rescued the inhibitory effect of miR-490-3p on (A) colony formation and (B) migration of AGS and TMK1 cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001, significantly different between indicated groups.