Supplementary Table 1A Amino acid substitutions within porcine parvovirus type 1 (PPV1) VP2 sequences

10 / 12 / 22 / 45 / 101 / 174 / 215 / 219 / 233 / 238 / 300 / 302 / 309 / 311 / 320 / 366 / 376 / 379* / 384* / 392 / 415 / 424 / 430 / 437* / 440 / 460 / 522 / 551 / 555 / 566 / 571
KP245931 / A / N / T / S / G / M / V / D / A / P / G / L / V / N / T / L / G / H / V / A / N / S / A / Y / I / H / P / A / R / H
KP245934 / A / N / T / S / G / M / V / D / A / P / R / L / A / N / T / L / G / H / V / A / N / S / A / Y / I / H / P / A / R / H
KP245935 / A / N / T / S / G / M / V / D / A / P / G / L / A / N / T / L / G / H / V / A / N / S / A / Y / I / H / P / A / R / H
KP245937 / A / N / N / S / G / M / V / D / T / P / G / L / A / N / T / Y / L / G / Q / V / A / K / S / A / Y / V / H / P / A / R / Y
KP245939 / A / N / N / S / G / V / V / D / T / P / G / L / A / D / T / Y / L / G / Q / V / A / K / S / A / Y / V / H / P / A / R / Y
KP245938 / P / N / N / S / A / M / T / N / S / L / G / L / A / N / I / F / G / Q / V / A / N / S / T / H / V / H / P / A / K / H
KP245930 / P / N / N / S / A / M / T / N / T / L / G / Q / A / N / I / F / G / Q / L / S / N / S / A / H / V / N / A / G / K / Y
KP245932 / P / N / N / S / A / M / T / N / S / L / G / Q / A / N / I / F / G / Q / L / A / N / P / A / H / V / N / A / G / K / Y
KP245933 / P / N / N / S / A / M / T / N / S / L / G / Q / A / N / I / F / G / Q / L / A / N / S / A / H / V / N / A / G / K / Y
NC001718 / P / N / N / T / A / M / I / N / S / L / G / Q / A / N / I / F / D / H / L / A / N / S / S / H / V / N / A / G / R / Y
Kresse / P / N / N / S / A / M / T / N / S / L / G / Q / A / N / I / F / G / Q / L / A / N / S / P / H / V / N / A / G / K / Y
KP245936 / P / Y / N / S / A / M / T / N / S / L / G / Q / A / N / I / F / G / Q / L / A / N / S / P / H / V / N / A / G / K / Y

*Sites considered responsible for the different tissue tropisms [3]

The yellow highlight indicates possible amino acid residues critical for differentiating the avirulent NADL-2 strain (accession no.: NC_001718) and the virulent Kresse strain (accession no.: U44978).

The green highlight shows the amino acid identity and differences among different strains.

The red residues are specific only to a few strains (KP245931, KP245934 and KP245935).

GenBank numbers in red stand for newly submitted sequences in this study.

Supplementary Table 1B Amino acid substitutions within porcine parvovirus type 2 (PPV2) capsid protein sequences

349 / 437 / 598 / 689 / 784 / 795/796 / 990
KP245947 / R / E / E / S / I / VE / P
KP245940 / R / E / E / S / I / IE / P
KP245941 / R / E / E / S / I / IE / P
KP245942 / R / E / E / S / I / VE / P
KP245943 / R / E / E / S / I / VE / P
KP245944 / R / E / E / S / I / IE / P
KP245946 / S / D / D / T / M / VQ / A
KP245945 / R / E / E / S / I / VE / P
GU938301 / R / E / E / S / I / VE / P
GU938300 / R / E / E / S / I / VE / P
GU938299 / R / E / E / S / I / VE / P
AB076609 / S / D / D / T / M / VQ / A

The green highlight shows the amino acid identity between AB076609 and KP245946.

The red residues are specific only to a few strains (KP245940, KP245941 and KP245944).

GenBank numbers in red stand for newly submitted sequences in this study.

Supplementary Table 1C Amino acid substitutions within porcine parvovirus type 3 (PPV3) capsid protein sequences

276 / 326 / 330 / 366
EU200671 / V / N / V / V
EU200672 / V / N / V / I
EU200673 / V / N / V / V
EU200677 / V / D / I / V
KP245948 / V / N / V / V
KP245949 / I / D / V / V
KP245950 / V / N / V / V
KP245951 / V / D / V / V
KP245952 / V / D / V / V

The yellow highlight shows the amino acid differences among different strains.

GenBank numbers in red stand for newly submitted sequences in this study.

Supplementary Table 1D Amino acid substitutions within porcine parvovirus type 4 (PPV4) capsid protein sequences

178 / 416 / 455 / 460 / 469 / 567 / 583 / 722
GQ387499 / S / E / E / R / I / G / T / Q
GQ387500 / S / K / E / R / I / E / A / Q
GU978964 / S / K / Q / K / I / E / T / Q
GU978965 / S / K / Q / K / I / E / T / Q
GU978966 / S / K / Q / K / I / E / T / P
GU978967 / P / K / Q / K / I / E / T / Q
GU978968 / P / K / Q / K / I / E / T / Q
HM031134 / S / K / Q / K / I / E / T / Q
HM031135 / S / K / Q / K / I / E / T / P
NC014665 / S / E / E / R / I / G / T / P
KP245953 / S / K / D / R / V / E / T / Q

The yellow highlight shows the amino acid nonidentity among different strains.

The red highlight indicates the reason for a separate branch of KP245953.

GenBank numbers in red stand for newly submitted sequences in this study.

Supplementary Table 1E Amino acid substitutions within porcine circovirus type 2 (PCV2) capsid protein sequences

8 / 47 / 53 / 57 / 59-60 / 63/4 / 68 / 72 / 76/7 / 80 / 86 / 88 / 89 / 90 / 91 / 101 / 121 / 130 / 131 / 133 / 134 / 136 / 151 / 169 / 185 / 187 / 190/1 / 206 / 210 / 215 / 217 / 232 / 234
KP245921 / F / T / I / V / KT / RT / N / M / IN / L / S / P / L / T / V / V / T / V / T / A / N / L / T / R / L / L / TG / I / D / I / M / N
KP245918 / F / T / I / V / KT / RT / N / M / IN / L / S / P / L / T / V / V / T / V / T / A / N / L / T / R / L / L / TG / I / D / I / L / N
KP245919 / F / T / I / V / KT / RT / N / M / IN / L / S / P / L / T / V / V / T / V / T / A / N / L / T / R / L / L / TG / I / D / I / M / N
KP245922 / F / T / I / V / KT / RT / N / M / IN / L / S / P / L / T / V / V / T / V / T / A / N / L / T / R / L / L / TG / I / D / I / M / N
KP245925 / F / T / I / V / KT / RT / N / M / IN / L / S / P / L / T / V / V / T / V / T / A / N / L / T / R / L / L / TG / I / D / I / M / N
HM038017 / F / T / I / V / KT / RT / N / M / IN / L / S / P / L / T / V / V / T / V / T / A / N / L / T / R / L / L / TG / I / D / I / M / N / K
AF055394 / Y / T / F / V / RT / KT / A / M / IN / L / S / P / R / S / V / V / S / V / T / A / T / L / T / S / L / L / TG / I / E / V / M / N
KP245920 / Y / T / F / I / RT / RT / A / M / IN / L / S / P / R / S / V / V / S / V / T / A / T / L / T / S / L / L / TG / I / E / V / M / N
KP245923 / Y / T / F / I / KT / RT / A / M / IN / L / S / P / R / S / V / V / S / V / T / A / T / L / T / S / L / L / TG / I / E / V / M / N
KP245924 / Y / T / F / I / RT / RT / A / M / IN / L / S / P / R / S / V / V / S / V / T / A / T / L / T / S / L / L / TG / I / E / V / M / N
KP245926 / Y / T / F / I / KT / RP / A / M / IN / L / S / P / R / S / V / V / S / V / P / T / T / L / T / S / L / L / TG / I / E / V / M / N
KP245927 / Y / T / F / I / RT / KT / A / M / IN / L / S / P / R / S / V / V / S / V / T / A / T / L / T / S / L / L / AG / I / E / V / M / N
KP245928 / Y / T / F / I / KT / RT / A / M / IN / L / S / P / R / S / V / V / S / V / T / A / T / L / T / S / L / L / TG / I / E / V / M / N
HM038034 / Y / T / F / V / AT / RT / A / M / LD / V / T / K / I / S / I / V / T / V / P / V / N / Q / P / S / L / L / SR / I / D / V / M / K
AF055392 / Y / T / F / V / RT / TT / A / M / ID / V / T / K / I / S / I / V / T / V / T / A / T / L / P / S / L / L / SG / K / D / V / M / K
KP245929 / Y / S / F / V / AS / TT / A / L / ID / L / T / K / I / S / I / I / S / F / P / S / T / L / P / S / M / I / SK / K / D / V / M / K

The red highlight indicates possible amino acid residues responsible for the distinction between traditional PCV2b and mutant PCV2b.

The green highlight shows the amino acid homology and differences among different strains.

GenBank numbers in red stand for newly submitted sequences in this study.