Online Resource 6A Stem-loop structure of pre-miR2118 derived from the sequence of a BAC/fosmid positive clone. Compared to the structure of the draft genome sequence-derived pre-miR2118, this structure demonstrates a stronger match with the miRNA criteria, with lower folding free energy (∆G) and only two nucleotide mismatches with the complement strand.


Online Resource 6B Stem-loop structure formation of pre-miR2118 derived from the draft genome sequence (Rahman et al. 2013). Compared to the structure of the BAC/fosmid library-derived pre-miR2118, this structure demonstrates a weaker match with the miRNA criteria, with higher folding free energy (∆G), four nucleotide mismatches with the complement strand and a G-to-A mutation.


Online Resource 6C Stem-loop structure of pre-miR6166 derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are two nucleotide mismatches with the complement strand.

Online Resource 6D Stem-loop structure of pre-miR6170 derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there is one nucleotide mismatch with the complement strand.



Online Resource 6E Stem-loop structure of pre-miR159a derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are three nucleotide mismatches with the complement strand.



Online Resource 6F Stem-loop structure of pre-miR159b derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are three nucleotide mismatches with the complement strand.


Online Resource 6G Stem-loop structure of pre-miR164a derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are three nucleotide mismatches with the complement strand.


Online Resource 6H Stem-loop structure of pre-miR164b derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are two nucleotide mismatches with the complement strand.


Online Resource 6I Stem-loop structure of pre-miR164c derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are three nucleotide mismatches with the complement strand.


Online Resource 6J Stem-loop structure of pre-miR164d derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are three nucleotide mismatches with the complement strand.


Online Resource 6K Stem-loop structure of pre-miR166a derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are four nucleotide mismatches with the complement strand.


Online Resource 6L Stem-loop structure of pre-miR166b derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are four nucleotide mismatches with the complement strand.


Online Resource 6M Stem-loop structure of pre-miR166c derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are three nucleotide mismatches with the complement strand.


Online Resource 6N Stem-loop structure of pre-miR167a derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are two nucleotide mismatches with the complement strand.


Online Resource 6O Stem-loop structure of pre-miR167b derived from the sequence of a BAC/fosmid positive clone. Folding free energy (∆G) is indicated, and there are two nucleotide mismatches with the complement strand.

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