RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA BANGALORE

SYNOPSIS

BY

Dr.BHAGYASHREE.B.CHOUGULE

FOR THE DEGREE OF AYURVEDA VACHASPATHI

M.D. AYURVEDA

IN

DRAVYA GUNA

TITLE OF THE TOPIC

“EVALUTION OF ANTI INFLAMMATORY ACTIVITY OF KANTAKARI(Solanum xanthocarpum Serb.&wende. ) .”

2007

NAME OF THE INSTITUTE

K.L.E.SOCIETY’S

B.M.KANKANWADI AYURVEDA MAHAVIDYALAYA

POST GRADUATE STUDIES AND RESEARCH CENTER

SHAHAPUR BELGAUM

FROM:

Dr.BHAGYASHREE.B.CHOUGULE

1st YEAR M.D.

Department of Post Graduate Studies

In DRAVYAGUNA

K.L.E’s Shri B.M.K.Ayurveda Mahavidyalaya

Shahpur Belgaum-590003

TO:

THE REGISTRAR

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES

KARNATAKA

JAYANAGAR,4TH ‘T’ BLOCK, BANGALORE.

THROUGH,

THE PRINCIPLE AND HEAD OF THE DEPARTMENT

IN DRAVYAGUNA

Post Graduate Studies And Research Center

K.L.E.’s Shri B.M.K.Ayurved Mahavidyalaya

Shahpur,Belgaum 590 003

SUB : Submission of the completed proforma for registration of subject for

Dissertation

Respected Sir,

I request you to kindly register synopsis on below mentioned subject against my name for the submission of dissertation to Rajiv Gandhi University of Health Sciences, Karnataka, Banglore for partial fulfillment of postgraduate course in the specialization of DravyaGuna M.D. (Ayurveda)

TITLE OF DISSERTATION :

“EVALUTION OF ANTI INFLAMMATORY ACTIVITY OF KANTAKARI(Solanum xanthocarpum Serb.&wende. )”

Here with I am enclosing the complete proforma for registration of subject for dissertation.

Thanking You,

Yours faithfully

Place:

Date: Dr.Bhagyashree.B.Chougule

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA BANGALORE.

COMPLETED PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION

1.NAME OF THE CANDIDATE: Dr.BHAGYASHREE.B.CHOUGULE

AND ADDRESS 1st YEAR M.D.

Post Graduate Department of Dravya-Guna

K.L.E’s B.M.K.Ayurveda Mahavidyalaya,

Shahpur, Belgaum-590003,

Karnataka

2.NAME OF THE INSTITUTE: SHRI B.M.K.MAHAVIDYALAYA,

SHAHPUR,BELGAUM-590 003.

3.COURSE OF STUDY : Ayurved Vachaspati (M.D.) in Dravya

guna

4.DATE OF ADMISSION : 08-06-2007

OF COURSE

5.TITLE OF THE TOPIC : EVALUTION OF ANTI-INFLAMMATORY

ACTIVITY OF “KANTAKARI”

( Solanum xanthocarpum Serb.&wende.)

6.BRIEF RESUME OF INTENDED WORK:

6.1 NEED FOR STUDY:

“Kantakari”(Solanum xanthocarpum Serb. &wende.) is one among the laghu panch moola1, also considered in gana like Shotha hara2, Angamardaprashamana, Kanthya & Kasahar. We get the classical reference of use of “Kantakari”(Solanum xanthocarpum Serb. &wende.) in the vyadhies like parshwapida, jwara, shwasa, kasa, pinasa & krimi3.

“Kantakari”(Solanum xanthocarpum Serb. &wende.) is one of the ingredients of yogas like Dashamoolasav, Dashamoolarishta & Dashamooladi-ghrita that is drug of choice in vata vikruti. “Kantakari” (Solanum xanthocarpum Serb. &wende.) is one among the dashamoola, which have the doshkarma as vataghnta; also it is explained in the context of Shothahara dashemani.

So, this study mainly intends to screen the Anti-inflammatory activity of “Kantakari-moola”(Solanum xanthocarpum Serb. &wende.) (In vitro).

6.2REVIEW OF LITERATURE.:

6)  Drug Review:

Botanical Name: Solanum xanthocarpum Serb.&wende.4

Family: Solanaceae

Gana: Shotha hara, Angamardaprashamana, Kanthya, Kasahara,

Sheetaprashamana, Hikkanigrahana5.

Brihatyadi, Varunadi,Laghupanchmoola6.

Morphology7:

A very prickly diffuse bright-green perennial herb, somewhat woody at base.

Roots8: The roots are long, fairly thick, hard and of light brown colour. The surface is not quite smooth being beset with many minute rootlets and a few lenticles. The thickness of the outer bark of the root is about 1/3rd the thickness of the central wood origin. The surface skin is quite thin & on scrapping a silvery white tissue is exposed. The wood is cream yellow & minutely porous. The entire root is bitter in taste.

Properties9, 10:

Rasa – Tikta, Katu

Guna – Laghu, Ushna, Tikshan

Virya – Ushna

Vipaka – Katu

Doshakarma – KaphaVatashamak

Karma-Shothahara, Vedanasthapana, Hridya, Deepani, Pachani

Distribution: The plant is found in all the plains as well as low hills through out India from Punjab & Assam to Cape Co Morin. In south India it is found abundantly along the Coromandel Coast & in the district of Tinnevelly & Kanyakumari. Every type of situation, which is not moist, seems to suit it, &it is commonly found growing wild as weed of roadside &waste land on rubbish heaps & similar situation near village

Chemical Constituent’s-Carotene, diosgenin, carpesterol, solasodine,

Solasonine, solasodino-L-rhamnosyl-B-D-glucoside11

Note(Previous works done):-

1)  Evalution of Solanum xanthocarpum Serb. &Wende extract as a synergist for cypermethrin against larvae of the filarial vector culex quinquefasciatus. By Lalit Mohan, Preeti Sharma & C.N.Srivastav Entomological Research 2006, Black well Synergy.

2)  Studies on hypoglycemic activity of Solanum xanthocarpum Serb. &Wende fruit extract in rats. By: - D.M.Kar, L.Maharana, S.Pattnaik, G.K.Dash. Journal of Ethno pharmacology.2006

3)  Solanum xanthocarpum Serb. &Wende (Kantakari) in chronic bronchitis, bronchial asthma& non-specific un-productive cough (An Experimental &Clinical co-relation) By:- N.P.Bector, A.S.Puri-J.Assoc.Physicians India 1971 (ncbi.nlm.nih.gov)

6.3 OBJECTIVE OF STUDY:

1.To evalute Anti-inflammatory activity of “Kantakari-moola” (Solanum xanthocarpum Serb. &wende.) (In vitro) in carrageenan induced edema.

7.MATERIALS AND METHODS:

7.1 Source of Data:

1)  Collection of “Kantakari-moola”(Solanum xanthocarpum Serb. &wende.) from the natural habitat.

2) Source of animal: From well-established animal house

7.2Method of collection data:

1.Pharmacognostic study of test drug

2.Prelimenary phytochemical evaluation for detection of alkaloids, tannins etc.

3.Qualitative confirmation through simple instrumental techniques.

4.Male Wistar rats will be divided into control and different treatment

Group (n=06s in each group)

Drug will be administered in single dose prior to induction of acute inflammation by injecting carrageenan (0.1ml of suspension in 0.9% saline) in sub-plantar region. Paw volume changes in ml will be monitored at 0, 0.5, 1, 3, 5 hours after injection carrageenan using plethysmograph.

Study Design:

A) Collected samples of “Kantakari”

(Solanum xanthocarpum Serb. &Wende.) will be subjected to following procedure:

i)  Identification, Collection & Authentification of Kantakari-moola.

ii)  Pharmacognostic study of ‘Kantakari-moola’.

a)  Macroscopic Study

b)  Microscopic Study

c)  Histochemistry

iii)  Physicochemical study of ‘Kantakri’ moola

a)  Moisture content

b)  Ash value

c)  Acid soluble/insoluble ash

d)  Water soluble/insoluble ash

iv)  Different solvent extraction:

a)  Aqueous extract

b)  Alcoholic extract

v)  Qualitative analysis by instrumental method

a) T.L.C.

b) H.P.T.L.C.

B) Animal study will be conducted as follow: -

i)  Dose Fixation

ii)  Healthy Wistar rats weighing between 200-250 grams will be procured and will be maintained in laboratory condition with normal diet and water for 3-5 days and divided into 3 groups each group containing 06 animals

01 / Group 01 / Control group –Distilled water with gum acacia will be given.
02 / Group 02 / Kashaya of test drug will be given.
03 / Group 03 / Standard drug Aspirin will be given.

Route Of Administration: Oral Route

a)  Procedure:

i) Acute inflammation will be induced by injecting carrageenan (0.1ml of 1% suspension in 0.9% saline) in sub-plantar region.

ii) Sub-acute inflammation will be induced by implanting sterile cotton pellet or sterile grass pith, subcutaneously in the ventral abdominal wall, under light anaesthesia with aseptic precaution.

b)  Treatment schedule

i) For acute inflammation: - Each rat in test group will be given test

drug one hour. Before being subjected to

edema.

ii) For sub-acute inflammation: - Treatment will be continued for

10 days

c) Observation: -

i)  For acute inflammation: - Paw volume changes in ml be monitored at 0, 0.5, 1, 3 and 5 hours after injecting carrageenan using plethysmograph.

ii)  For sub-acute inflammation:- Granuloma will be removed on the 11th day to note their histology.

Results will be analyzed by statistical method (Students t-test and if required ANNOVA test)

7.3 Does the study require any investigations or interventions conducted on animals, patients or humans? (If so describe briefly)

The experiment will be carried out on individually housed wistars rats colony that are maintained on standard pattern of food, water and temperature.

7.4 Has the ethical clearance been obtained from your institution?

Yes, Enclosed.

8. LIST OF REFERENCES:

1. “SUSRUT SAMHITA” by Sushrutaacharya with Nibandhsangraha commentary of Shri Dalhanaacharya, Edited by Yadavji Trikamji, Sutrasthana adhyaya 38th shloka no.66, ChaukhambaOrientalia, Varanasi, 14th Edition, page no.169.

2. “CHARAK SAMHITA” by Agnivesh Revised by Charak & Dridhbala with Ayurveda-dipika Commentary of Chakrapanidatta, Edited by Yadvaji Trikamji, sutrasthana adhyaya 4th shloka no.38, Choukhamba Vishwabharti,Varanasi (UP), Reprint 1984, page no.34

3. “KAIDEV NIGHANTU” Edited & Translated by Sharma P.V. & Sharma G. Aushdhi varga Shloka no.54-55, Varanasi, ChaukhambhaOrientalia, 1st sssEdition 1979,Page no.13.

4.“Indian Materia Medica” by Nadkarni K.M, Vol-1, Popular Prakashana Private Limited, Reprint 2002,Page no.1156.

5“CHARAK SAMHITA” by Agnivesh Revised by Charak & Dridhbala with Ayurveda-dipika Commentary of Chakrapanidatta, Edited by Yadvaji Trikamji, sutrasthana adhyaya 4th shloka no.38, Choukhamba Vishwabharti,Varanasi (UP), Reprint 1984, page no.34

6.“SUSRUT SAMHITA” by Sushrutaacharya with Nibandhsangraha commentary of Shri Dalhanaacharya, Edited by Yadavji Trikamji, Sutrasthana adhyaya 38th shloka no.66, 67, ChaukhambaOrientalia, Varanasi, 14th Edition, page no.169.

7.“Indian Medicinal Plant” Vol-3 by Kirtikar.K.R. & Basu.B.D., Lalit Mohan Basu, Allahabad, Reprint-1999, Page no.1759.

8. “Pharmacognosy of Ayurvedic drugs” by Iyer.K.N. & Kolammal. M., Series-1 number-4, 1960,Trivendrum, Department of Pharmacognosy, University of Kerala. Page no.93.

9. “Bhavaprakasha Nighantu” by Bhavamishra, Edited by Chunekar K.C. Guduchyadi varga, Varanasi, Choukhamba Vishwabharti Academy, Page no.290.

10.Pandey.G.’s “Dravya-Guna Vijanan” Vol.2, Varanasi, Krishnadas Academy, First Edition 2001, Page no.84.

11.Shastry.J.L.N’s “Dravyaguna Vijananam” Vol.2, Varanasi, Chaukhambha Orientalia, Second edition 2005,Page no.368

9. SIGNATURE OF THE CANDIDATE :

10. REMARKS OF THE GUIDE : Kantakari being one among the

shothahara dashemani, the study

is planed to evaluate its anti-

inflammatory activity in Wistar

strain albino rats

11. NAME AND DESIGNATION OF

11.1 GUIDE : Dr.S.K.Hiremath.B.A.M.S.,MD.

Professor & HOD

Dept. of Dravya Guna

K.L.E’s B.M.K.Ayurveda

Mahavidyalaya,

Shahpur, Belgaum-590003,

Karnataka

11.2 SIGNATURE :

11.3 CO-GUIDE : Dr.P.A.Patil.M.B.B.S.,M.D.(Pharma.)

Pro Belgaum fessor & HOD

Dept. of Pharmacology

J.N.Medical College

Belgaum

11.4 SIGNATURE :

11.5 HEAD OF THE DEPARTMENT : Dr.S.K.Hiremath.B.A.M.S.,MD.

Professor & HOD

Dept. of Dravya Guna

K.L.E’s B.M.K.Ayurveda

Mahavidyalaya,

Shahpur, Belgaum-590003,

Karnataka

11.6 SIGNATURE :

12 REMARKS OF THE CHAIRMAN :The above synopsis isscrutinized by

AND PRINCIPAL Guide,HOD,and accorded by Internal

Ethical Committee and recommended

for the registration of subject for

dissertation

12.1 SIGNATURE :

7