Cloning of Sec16
Original construct obtained from Kazusa DNA research institute.
Plasmid is pBC SKplus.
On Pubmed, it probably corresponds to AB002308.
Primers designed
KIAA0310 f1
GGGGAGATCTATGCAGCCACCGCCCCAGACGG
Contains a BglII site just before the ATG of KIAA0310
KIAA0310 r1
GGGGGAATTCTCAGTTCAGCACCAGGTGCTTCC
Contains an EcoRI site.
Changes the stop codon to a TGA
Full length KIAA0310 was pcr’d from the original pBC SKplus KIAA0310 clone.
This PCR product was then cloned into the pGEM-T-Easy vector.
From this, the BglII/EcoRI KIAA0310 cut fragment was purified, and ligated into BglII/EcoRI cut pEGFP-C1.
LOCUS AB002308 9031 bp mRNA linear PRI 06-AUG-2005
DEFINITION Homo sapiens mRNA for KIAA0310 protein, partial cds.
ACCESSION AB002308
VERSION AB002308.4 GI:71891789
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Catarrhini;
Hominidae; Homo.
REFERENCE 1
AUTHORS Nagase,T., Ishikawa,K., Nakajima,D., Ohira,M., Seki,N.,
Miyajima,N., Tanaka,A., Kotani,H., Nomura,N. and Ohara,O.
TITLE Prediction of the coding sequences of unidentified human genes.
VII. The complete sequences of 100 new cDNA clones from brain which
can code for large proteins in vitro
JOURNAL DNA Res. 4 (2), 141-150 (1997)
PUBMED 9205841
REFERENCE 2 (bases 1 to 9031)
AUTHORS Ohara,O., Nagase,T., Kikuno,R. and Nomura,N.
TITLE Direct Submission
JOURNAL Submitted (28-MAR-1997) Osamu Ohara, Kazusa DNA Research Institute;
Kazusa-kamatari 2-6-7, Kisarazu, Chiba, 292-0818, Japan
(E-mail:, Tel:81-438-52-3913)
COMMENT On Aug 5, 2005 this sequence version replaced gi:20520992.
FEATURES Location/Qualifiers
source 1..9031
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="ee03470"
/cell_type="normal human aortic endothelial cells"
/note="vector:pBC SK plus. This sequence was replaced that
of hg00111s1 cDNA as a representative cDNA sequence for
KIAA0310."
gene <1..9031
/gene="KIAA0310"
CDS <217..7383
/gene="KIAA0310"
/note="Start codon is not identified."
/codon_start=1
/product="KIAA0310 protein"
/protein_id="BAA20769.4"
/db_xref="GI:71891790"
/translation="PPESLGLQLQLRNSYILLPSVSSIDLCKGSVMQPPPQTVPSGMA
GPPPAGNPRSVFWASSPYRRRANNNAAVAPTTCPLQPVTDPFAFSRQALQSTPLGSSS
KSSPPVLQGPAPAGFSQHPGLLVPHTHARDSSQGPCEPLPGPLTQPRAHASPFSGALT
PSAPPGPEMNRSAEVGPSSEPEVQTLPYLPHYIPGVDPETSHGGHPHGNMPGLDRPLS
RQNPHDGVVTPAASPSLPQPGLQMPGQWGPVQGGPQPSGQHRSPCPEGPVPSGVPCAT
SVPHFPTPSILHQGPGHEQHSPLVAPPAALPSDGRDEVSHLQSGSHLANNSDPESTFR
QNPRIVNHWASPELRQNPGVKNEHRPASALVNPLARGDSPENRTHHPLGAGAGSGCAP
LEADSGASGALAMFFQGGETENEENLSSEKAGLSGQADFDDFCSSPGLGRPPAPTHVG
AGSLCQALLPGPSNEAAGDVWGDTASTGVPDASGSQYENVENLEFVQNQEVLPSEPLN
LDPSSPSDQFRYGPLPGPAVPRHGAVCHTGAPDATLHTVHPDSVSSSYSSRSHGRLSG
SARPQELVGTFIQQEVGKPEDEASGSFFKQIDSSPVGGETDETTVSQNYRGSVSQPST
PSPPKPTGIFQTSANSSFEPVKSHLVGVKPFEADRANVVGEVRETCVRQKQCRPAAAL
PDASPGNLEQPPDNMETLCAPQVCPLPLNSTTEAVHMLPHAGAPPLDTVYPAPEKRPS
ARTQGPVKCESPATTLWAQSELPDFGGNVLLAPAAPALYVCAKPQPPVVQPPEEAMSG
QQSRNPSSAAPVQSRGGIGASENLENPPKMGEEEALQSQASSGYASLLSSPPTESLQN
PPVLIAQPDHSYNLAQPINFSVSLSNSHEKNQSWREALVGDRPAVSSWALGGDSGENT
SLSGIPTSSVLSLSLPSSVAQSNFPQGSGASEMVSNQPANLLVQPPSQPVPENLVPES
QKDRKAGSALPGFANSPAGSTSVVLVPPAHGTLVPDGNKANHSSHQEDTYGALDFTLS
RTLENPVNVYNPSHSDSLASQQSVASHPRQSGPGAPNLDRFYQQVTKDAQGQPGLERA
QQELVPPQQQASPPQLPKAMFSELSNPESLPAQGQAQNSAQSPASLVLVDAGQQLPPR
PPQSSSVSLVSSGSGQAAVPSEQPWPQPVPALAPGPPPQDLAAYYYYRPLYDAYQPQY
SLPYPPEPGAASLYYQDVYSLYEPRYRPYDGAASAYAQNYRYPEPERPSSRASHSSER
PPPRQGYPEGYYSSKSGWSSQSDYYASYYSSQYDYGDPGHWDRYHYSARVRDPRTYDR
RYWCDAEYDAYRREHSAFGDRPEKRDNNWRYDPRFTGSFDDDPDPHRDPYGEEVDRRS
VHSEHSARSLHSAHSLASRRSSLSSHSHQSQIYRSHNVAAGSYEAPLPPGSFHGDFAY
GTYRSNFSSGPGFPEYGYPADTVWPAMEQVSSRPTSPEKFSVPHVCARFGPGGQLIKV
IPNLPSEGQPALVEVHSMEALLQHTSEQEEMRAFPGPLAKDDTHKVDVINFAQNKAMK
CLQNENLIDKESASLLWNFIVLLCRQNGTVVGTDIAELLLRDHRTVWLPGKSPNEANL
IDFTNEAVEQVEEEESGEAQLSFLTGGPAAAASSLERETERFRELLLYGRKKDALESA
MKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAASTCCGD
EKWGDWRPHLAMVLSNLNNNMDVESRTMATMGDTLASRGLLDAAHFCYLMAQAGFGVY
TKKTTKLVLIGSNHSLPFLKFATNEAIQRTEAYEYAQSLGAETCPLPSFQVFKFIYSC
RLAEMGLATQAFHYCEAIAKSILTQPHLYSPVLISQLVQMASQLRLFDPQLKEKPEEE
SLAAPTWLVHLQQVERQIKEGAGVWHQDGALPQQCPGTPSSEMEQLDRPGLSQPGALG
IANPLLAVPAPSPEHSSPSVRLLPSAPQTLPDGPLASPARVPMFPVPLPPGPLEPGPG
CVTPGPALGFLEPSGPGLPPGVPPLQERRHLLQEARSPDPGIVPQEAPVGNSLSELSE
ENFDGKFANLTPSRTVPDSEAPPGWDRADSGPTQPPLSLSPAPETKRPGQAAKKETKE
PKKGESWFFRWLPGKKKTEAYLPDDKNKSIVWDEKKNQWVNLNEPEEEKKAPPPPPTS
MPKTVQAAPPALPGPPGAPVNMYSRRAAGTRARYVDVLNPSGTQRSEPALAPADFVAP
LAPLPIPSNLFVPTPDAEEPQLPDGTGREGPAAARGLANPEPAPEPKVLSSAASLPGS
ELPSSRPEGSQGGELSRCSSMSSLSREVSQHFNQAPGDLPAAGGPPSGAMPFYNPAQL
AQACATSGSSRLGRIGQRKHLVLN"
ORIGIN
1 gcggagaccc gagccggatg tgccaagatg gctgcggcgg ctgaggtgtc tgtgctcgtc
61 gccagcgtcg ggtgggcttt cgcccgcggc tcctgaggga tcggtctcag ccgcgcggag
121 accgtcttgc tctgttgccc aggctggacc gcggtggtat aatcatagct cactgcagcc
181 tcgaactcat ggacacaagt gatcttcctt ccttaacccc ctgagtccct gggactacag
241 ctccaattaa ggaacagcta tatcctgctt ccttctgtaa gcagcatcga cttgtgcaag
301 ggttcagtca tgcagccacc gccccagacg gtcccgtctg gcatggctgg gccacctcca
361 gccgggaatc ctcggagcgt gttctgggct agcagccctt acaggagacg ggctaataat
421 aatgcagcag tggctccgac aacttgcccg ttgcagccgg tcacggatcc atttgctttt
481 agtagacagg cgctccaaag tacaccactg ggcagttcgt ccaaaagcag tccacctgtc
541 ttgcaaggcc cagcccccgc agggttttct cagcaccccg gtttgcttgt tcctcacaca
601 catgccagag atagctctca gggaccctgt gagcccctgc ctggacctct gacacagccc
661 agagcacatg ccagtccgtt ttctggtgca ttgacacctt cagcacctcc tgggcctgag
721 atgaacagga gtgcagaggt cggtcccagt tcagagcctg aagttcagac tctgccatat
781 cttcctcact acattccagg agtggatcct gaaacgtctc atgggggcca ccctcatggg
841 aacatgcctg ggctcgaccg acccctgagc aggcaaaacc cacatgacgg tgtggtcacc
901 ccagcagcat ccccttccct ccctcagcct ggtctgcaga tgccaggaca gtgggggcca
961 gtgcagggag gcccacagcc ctcggggcaa catcgttcac cctgccctga aggacctgtt
1021 cccagcgggg tgccctgtgc caccagcgtt cctcatttcc ccaccccgtc catcctacat
1081 cagggccctg gtcatgagca acacagccct ctggtggctc ccccagcagc cttgcccagt
1141 gacggaagag acgaggtgag ccacttgcaa agtggaagcc acctggccaa taactctgat
1201 cctgaaagta cattcaggca aaatcccaga attgtgaatc actgggcaag cccagagctc
1261 aggcagaatc caggagtgaa gaatgagcac cggcccgcct ctgctcttgt gaaccccctc
1321 gcccggggag atagcccaga aaaccgtacg caccacccac tgggggctgg ggccgggtct
1381 ggctgtgccc cgctagaagc agactcagga gcttcaggag ctctggcgat gtttttccaa
1441 gggggagaga cagaaaatga ggagaatctc tcatctgaaa aagcaggctt atctggtcaa
1501 gcggactttg acgatttctg ctccagccct gggctaggcc gtccgcccgc acctacacac
1561 gtgggggcag gcagcctctg ccaggccctt ctcccaggcc ccagcaatga ggctgctggt
1621 gatgtgtggg gtgacacagc gagcacaggg gtgccggatg ccagcggctc gcagtatgag
1681 aatgttgaga acttagaatt tgttcagaat caagaagttc tgccaagtga gcccctcaat
1741 ttggaccctt cctccccgag tgaccagttc agatatgggc cccttcctgg gccagctgtg
1801 cccaggcatg gtgctgtgtg ccacaccgga gcccctgatg ccacactgca tacagtgcac
1861 cctgacagcg tgtcatccag ctatagcagc agaagccacg gaaggctctc aggctcagcc
1921 aggccccagg agctggttgg cacattcatt cagcaagaag ttggaaaacc cgaggatgaa
1981 gcttcaggta gtttttttaa gcaaatcgat tcttctcccg taggaggtga aacagacgag
2041 accactgtga gccagaatta ccgtggcagc gtgtcccagc cctcaacccc gagccccccg
2101 aaacctacag gaatatttca gacaagtgca aatagttctt tcgaaccggt aaaatctcac
2161 ttagttgggg taaaaccatt tgaggcagat cgcgccaacg tggttggtga agtaagggag
2221 acctgtgtcc gccagaagca gtgcagacca gctgccgccc tgcccgatgc ttcccctggc
2281 aacctggagc agccaccaga caacatggag accctctgtg caccccaggt ctgtcccctg
2341 cctcttaact ccaccacgga agctgtgcac atgcttccgc acgcaggggc accgcccttg
2401 gatactgtgt atccagcacc cgagaagagg ccttcagcca ggacccaggg gcccgtgaag
2461 tgtgagagcc cagcaacgac tctgtgggcg caaagtgagc tgccagattt tggaggcaac
2521 gtccttctgg cccctgcagc cccggcgctt tatgtgtgtg caaaacctca gccacctgtt
2581 gttcagcctc cagaagaggc gatgtccggg cagcagtcac ggaacccaag ctcggcggcc
2641 ccggtgcaga gccgaggtgg cattggtgct tctgagaacc ttgagaatcc tcccaaaatg
2701 ggagaggagg aggcccttca gtcccaggcg agttctggtt atgcaagttt attatcctca
2761 ccgcccactg agtctctgca gaatcctcca gtcttgattg ctcagcctga tcacagctat
2821 aatctggctc agcccattaa cttttctgtg tccttatcga actctcatga gaagaatcag
2881 tcctggagag aggctttggt gggggataga cctgcagtca gcagttgggc tctcggtggt
2941 gattctgggg agaacacttc tttgtctggg attccaacca gctctgtcct tagcttgtct
3001 ctgcctagca gtgttgccca aagtaatttt ccacaaggtt ctggtgcttc cgaaatggtt
3061 tctaatcagc ctgctaattt gctggttcaa ccaccatccc agccagttcc agagaacttg
3121 gttccagaaa gtcaaaagga tcgtaaggca ggaagtgctc ttcccggatt tgctaatagc
3181 cctgctggaa gcacaagtgt ggtgttagtt ccacctgcac acggcaccct ggtgcctgat
3241 ggtaataagg caaaccattc cagtcatcag gaagacactt acggagccct agactttacc
3301 ttaagcagga ctttggaaaa tcctgtaaac gtgtacaacc cgtcccattc tgacagcctc
3361 gcttctcagc aaagtgttgc cagtcatccc agacaatctg ggcctggggc gcctaacctt
3421 gaccgttttt atcagcaggt cacgaaagat gcccagggcc agcctggcct cgaaagagcc
3481 cagcaggagc tggtgccacc ccagcaacag gcttctcccc cacaactacc caaagccatg
3541 ttttcggagc tgtcaaatcc agaaagtctg cccgcacagg gacaggccca gaactcagca
3601 cagtcaccag caagtctggt tctggtcgac gcgggtcagc agctgccccc tcggcctcct
3661 cagtcctcta gcgtgtctct ggtgtccagt ggctccggcc aggcagctgt gccgtcagag
3721 cagccgtggc cacagccagt gcctgcactt gcccccggcc caccgcctca ggacctggcc
3781 gcctactact actaccggcc tttgtacgat gcctaccagc ctcagtactc tttgccgtac
3841 ccaccggagc ctggcgcagc ctccctctat taccaggatg tctacagcct ctatgagcct
3901 cgatacaggc cctatgatgg tgctgcgtct gcttacgccc agaactaccg ctatcccgag
3961 cccgagcggc ccagctcccg agccagccac tcctcggaac ggccacctcc caggcaagga
4021 tatcctgaag gatactatag ttccaaaagt ggatggagca gtcagagcga ttactatgca
4081 agctattact ccagccagta cgattatgga gatccaggtc actgggatcg ttaccactac
4141 agtgctagag tcagggaccc ccgcacctat gaccggaggt attggtgtga tgcagagtat
4201 gacgcataca ggagagagca ctctgccttc ggggacaggc ccgagaaacg tgacaacaac
4261 tggaggtacg atcctcgctt cacggggagt tttgacgatg accccgatcc gcacagagac
4321 ccttatgggg aagaggtgga ccggcgcagc gtccacagcg agcactcggc acggagcctg
4381 cacagcgcac acagcctggc cagccgccgc agcagcctca gctcccactc gcaccagagt
4441 cagatttaca gaagccacaa tgtggctgcc ggttcctacg aggccccgct tcctccaggc
4501 tcctttcacg gcgattttgc ctacggcacc taccgcagca atttcagcag tggccccggc
4561 ttcccagagt atggctaccc tgccgacacc gtctggcctg ccatggagca agtttcatca
4621 agaccaactt ctcctgaaaa attttcagtg cctcatgtct gtgccaggtt tggccctggc
4681 ggtcagctta tcaaagtgat tcccaatctg ccttcagaag gacagccggc cttggtggag
4741 gtccacagca tggaggcctt gctgcagcac acgtctgagc aggaggagat gcgggcgttc
4801 ccgggacccc tggccaaaga cgacacccat aaggtggatg tcattaattt tgcacagaac
4861 aaagctatga aatgtttgca gaatgaaaac ttaattgaca aagagtctgc aagtcttctt
4921 tggaatttta ttgttctctt atgcagacaa aatgggaccg tggtagggac cgacattgcg
4981 gagcttctgt tacgagacca cagaacagtg tggcttcctg ggaagtcgcc caatgaagca
5041 aacctgattg atttcacgaa tgaggcagtg gagcaggtgg aagaggagga gtctggtgag
5101 gcccagctct ctttcctcac tggtggtccg gcggctgccg ccagctcgct cgagagagag
5161 accgagaggt tcagggagct gttgctgtat ggccgtaaga aggatgcttt ggagtctgca
5221 atgaagaatg gcctgtgggg tcacgctctg ctacttgcaa gtaagatgga cagccggaca
5281 cacgcccgag tcatgaccag gtttgctaac agcctcccaa tcaacgaccc tctgcagaca
5341 gtctaccagc tcatgtccgg acggatgcct gccgcgtcca cgtgctgtgg agacgagaaa
5401 tggggagatt ggaggccgca cctcgccatg gtcttgtcca acttgaacaa caacatggac
5461 gtcgagtcca ggacgatggc taccatgggc gacactctgg cttcaagggg cctcttggat
5521 gcggcccact tctgctacct catggcccag gcgggatttg gtgtttacac gaagaaaact
5581 acaaagcttg tcttaatcgg atccaatcac agtttgccat tcttaaagtt cgcaaccaac
5641 gaagcaatcc agaggacgga agcctatgag tacgcccagt ccctgggtgc cgagacctgc
5701 cccctgccta gtttccaggt gtttaagttc atctactcct gccgcctggc ggaaatgggg
5761 ctggccacgc aagccttcca ctactgtgag gccatcgcga agagcatcct gacgcagccg
5821 cacctgtatt ccccggtgtt gatcagccag cttgtgcaga tggcttccca gttacgactc
5881 ttcgatcccc agctgaaaga gaagccagaa gaggagtcct tggccgcacc cacgtggctg
5941 gttcacctgc agcaggtgga gcggcagatt aaggaggggg ctggagtatg gcatcaggat
6001 ggagccctcc cgcagcagtg tcctggcact ccgagttccg agatggagca gttggacagg
6061 ccaggactca gtcagccagg agccctgggg atcgccaacc ctctgctggc ggtgcctgca
6121 ccgagccctg agcactcgag cccgagcgtg cggctgctgc cctcagctcc gcagacgctc
6181 cctgacggcc cattggccag tcctgccaga gtgccgatgt tcccagtgcc actgcccccg
6241 gggcccctgg agccgggtcc tggctgtgtg accccagggc ctgcacttgg cttcctggag
6301 ccctccgggc ctggcctccc acctggtgtg ccacctctgc aggaaaggag acacttgctc
6361 caggaagcca ggagcccaga cccagggata gtgccgcagg aggcgcctgt tggaaactca
6421 ctttccgagc taagcgaaga aaattttgat ggaaaatttg ctaatctgac cccctcgagg
6481 acggtgccag actcggaggc ccccccaggg tgggatcgtg ccgactcggg tcccacgcag
6541 ccacctctgt ctctctcacc cgctcccgaa acaaagagac ccggacaggc agccaagaaa
6601 gaaacgaagg aacctaagaa gggtgaatcc tggttctttc gttggctacc tggaaagaaa
6661 aagacagaag cttatttgcc agatgacaag aacaaatcga ttgtttggga tgaaaagaaa
6721 aaccagtggg tgaatttaaa tgagccagaa gaggagaaga aagccccgcc cccacctcca
6781 acctcgatgc ccaagactgt gcaagctgcc ccgcctgccc tcccagggcc tcctggagcc
6841 cccgtgaaca tgtactctag aagagcagca ggaaccagag ctcgctacgt tgacgtcctg
6901 aacccaagcg ggacccagcg gagcgagccg gctctcgctc ctgcggactt tgtcgctcca
6961 ctcgcgccac tcccaattcc ttctaacttg ttcgtgccaa ccccagatgc agaagaacca
7021 cagcttccag acgggactgg cagggaaggg cctgcagcag ctaggggcct ggccaatcca
7081 gagcctgccc cagagcccaa ggttttaagc tctgcagcgt cactccctgg ctctgaactc
7141 ccctcctcca ggcctgaggg ttcccaggga ggagagcttt cgcgctgtag ttcaatgagt
7201 tcattatcac gtgaagtgag ccagcatttt aatcaggctc ctggcgacct ccctgctgca
7261 gggggccctc ccagcggggc catgcccttc tacaaccctg ctcagctggc acaggcctgc
7321 gccacctccg ggagctcaag gctagggagg attggccaga ggaagcacct ggtgctgaac
7381 taggcttgcc ctgctgtgaa cttgcacttg gagccctgac gctgctgttc tccccgaaga
7441 acccgaccga cctccgcgat ctccgtcccg cccccaggga gacacagcag tgactcagag
7501 ctggtcgcac actgtgcctc cctcctcacc gcccatcgta atgaattatt ttgaaaatta
7561 attccaccat cctttcagat tctggatgga aagactgaat ctttgactca gaattgtttg
7621 ccgaaaagaa tgatgtgact ttcttagtca tttaggatga tttaaggata tagtattcct
7681 ggtcatttaa gaatgttcat tcattgaagc cggagctgtc tctgccacag gagagccaca
7741 tggtcggtag taaccagggc ctctccaagc ccagctgtga gtcactgccc agtgagtccc
7801 gcgcttcctt taaggtgctg ggagcaaaga gagggtgact gaggcagacc ccaacccctg
7861 ctctgcacca tctgggccct cgccgtgttt gaacctggct gaatgagtgg agggcgctgt
7921 gttctcaatc agcgcctccg aggagccgtg gggttccttc ggcattagtt cacggttttt
7981 gagagaggcc ttagttactg cagtgaattt ctttcctgtt gcaaagacgc ttccagcctc
8041 actttacttt ctgtggcctg atgaggacca tgggtgattt tgtgtaccca aagcgctggg
8101 gactgcccac cgtgtggccc agtcactggg aaggagcccc agagagccgg ctgtctgaca
8161 tgatggctca gggtggtcat ccaggttgaa aactgaccgt gtgatgtttg atttgggctt
8221 catttcgtgt gtaggagcac ggttagactc actgttaagg aagctggatg cacttctcta
8281 aaaggctgca ctttccgtga gcacttttcg tggtacaatc cacatgaccc actttctccc
8341 ctgggggacg ttggttcaga ggttggtagc acttggggag agtatcttaa cacagtttct
8401 tgacagcagc tctggaactt agtatttctg ccccgagttt tgccacactg agactttgag
8461 tagctcctgg tggactcaac cctgttcaac tcagagacgg gcctcctctc actgatgcaa
8521 agctttaagg cttctctgac tgttctgaaa ctcttcgtat tcttgtcaag tctaaagaga
8581 ctgaagaaaa gatttaaata ctaataaaaa tcagtagata atttctgtag gttctgctgg
8641 aggaatacaa actgtttggt gttttaaatt taagtgtaga aattgtagaa tgtggaatta
8701 gcacagatcc ttcctggctt tctgtttcac ttgatcattt agcccagacc acccaggatg
8761 ttttccaaaa tgttccacag gcgtgtcccg ctggatccat ttgtccttgt cacttggaga
8821 aaggccagtc cctgtgacgg ggcagccctc tctgtccctc ggtcagctcg tgtgaatcct
8881 gggacctctt ccggtcggct ctgcccgctg ttctggggtc gactgccacg acttttgatt
8941 caagaagctt cctccaggcg ggagcggcta tttttcctaa atgagaattg ttacattgca
9001 aattgttgaa taaaatattt tgcgctcctt c
//