TABLES
Table S1. Substrate characterization
Constituents / Cattle dung(Starter) / Park wastes
Fresh leaves / Dry leaves
Total solid (%) / 17.0 / 35.0 / 84.0
Volatile solid (%) / 64.5 / 81.2 / 59.5
Organic carbon (%) / 37.4 / 47.1 / 34.5
Total nitrogen ( %) / 2.8 / 1.5 / 0.8
C/N / 13.6 / 31.0 / 45.4
pH / 7.2 / 6.8 / 5.8
EC / 1.33 / 2.9 / 2.1
Table S2. Daily and cumulative production of biogas, methane and CO2 in untreated and pretreated substrate
Cumulative production(l/KgVS) / Daily production
(l/KgVS) / RT
(days)
Pretreated / Untreated / Pretreated / Untreated
CO2 / CH4 / Biogas / CO2 / CH4 / Biogas / CO2 / CH4 / Biogas / CO2 / CH4 / Biogas
0.0±0.00a / 0.0±0.00a / 0.00±0.00a / 0.0±0.00a / 0.0±0.00a / 0.00±0.00a / 0.0±0.00a / 0.0±0.00a / 0.00±0.00a / 0.0±0.00a / 0.0±0.00a / 0.00±0.00a / 0
±0.26b2.1 / ±0.95b2.1 / ±1.11b4.2 / ±0.05b2.1 / ±0.08b1.8 / ±1.05b3.9 / ±0.05b1.0 / ±0.08b0.7 / ±0.11b1.7 / ±0.08b0.8 / ±0.03b0.7 / ±0.10b1.5 / 7
6.2±0.75c / 6.4±1.13c / 12.6±0.39c / 6.6±0.35c / 6.3±0.22c / 12.9±0.35c / 0.9±0.10c / 1.5±0.17c / 2.4±0.26c / 0.9±0.13c / 1.2±0.05c / 2.1±0.12c / 14
±1.41d10.2 / ±2.42d17.2 / ±2.41d27.4 / ±1.11d12.2 / ±1.14d15.6 / ±2.33d27.8 / ±0.14d0.8 / ±0.13c1.4 / ±0.35c2.2 / ±0.05b0.7 / ±0.09c1.2 / ±0.11c2.0 / 21
17.9±1.06e / 29.9±3.03e / 47.8±3.09e / 17.8±1.22e / 25.3±0.91e / 43.1±2.12e / 0.8±0.23e / 1.9±0.63d / 2.6±0.85c / 0.8±0.01bb / 1.3±0.15c / 2.1±0.15c / 28
±1.88f24.2 / .0±2.84f43 / ±3.76f67.2 / ±1.02f24.6 / ±2.32f36.8 / ±3.40f61.4 / ±0.16c0.9 / ±0.20c1.5 / ±0.22c2.4 / ±0.04c0.9 / ±0.10c1.1 / ±0.13c2.0 / 35
29.4±1.95g / 54.4±5.24g / 83.7±7.07g / 28.8±1.46g / 45.1±1.17g / 73.9±2.55g / 0.7±0.27e / 1.5±0.41c / 2.2±0.65c / 0.6±0.13d / 1.3±0.07c / 1.9±0.18c / 42
±2.41h37.9 / ±4.91h66.2 / ±7.52h104.1 / ±1.23h32.6 / ±2.05h53.0 / ±2.25h85.6 / ±0.10c0.9 / ±0.15c1.4 / ±0.15c2.3 / ±0.07c0.9 / ±0.05bd0.7 / ±0.11bc1.6 / 49
41.2±2.02hi / 75.8±8.32i / 117±10.35i / 36.1±1.19i / 58.4±1.35hi / 94.6±2.2hi / 0.9±0.32c / 1.5±0.42c / 2.4±0.71c / 0.5±0.18e / 0.8±0.13d / 1.3±0.30d / 56
±2.27i44.9 / ±8.16i77.3 / ±9.44i122.2 / ±1.44ij37.4 / ±1.19i59.4 / ±3.57i96.8 / ±0.12e0.7 / ±0.10b0.7 / ±0.12d1.4 / ±0.11f0.4 / ±0.14e0.3 / ±0.09e0.7 / 63
46.1±2.98i / 79.8±9.1i / 125.9±12.02i / 41.2±1.04j / 61.4±0.93i / 102.6±1.77i / 0.0±0.00a / 0.0±0.00a / 0.00±0.00a / 0.2±0.06g / 0.0±0.00a / 0.2±0.06f / 70
340.34 / 111.61 / 162.21 / 235.41 / 2531.30 / 1716.37 / 14.78 / 15.99 / 16.43 / 30.31 / 150.63 / 98.01 / F value
0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / 0.0001 / P value
Values are the mean of three replicates ± SD.
Values with the same letter in the same column are insignificant (p≤0.05)
Table S3. Physico-chemical properties of untreated and pretreated substrate
Pretreated / Untreated / RT(day)
C/N / EC (dSm-1) / pH / C/N / EC (dSm-1)* / pH
26.1±0.05a / 3.2±0.03a / 7.5±0.22a / 28.8±0.03a / 2.6±0.02a / 7.1±0.33a / 0
±0.07ab27.9 / ±0.05ab2.8 / ±0.12ab7.4 / ±0.04a25.1 / ±0.05ab3.4 / ±0.15a6.9 / 7
23.7±0.03b / 3.6±0.30b / 7.3±0.35ab / 27.1±0.06ab / 2.9±0.01b / 7.0±0.21a / 14
±0.06c25.0 / ±0.09bc3.1 / ±0.15ab7.0 / ±0.01b21.2 / ±0.02b3.8 / ±0.20ab6.7 / 21
19.4±0.06c / 3.9±0.01bc / 6.7±0.15b / 24.1±0.04b / 3.2±0.06bc / 6.5±0.15b / 28
±0.09d23.0 / ±0.06c3.3 / ±0.11b6.8 / ±0.05bc16.2 / ±0.05bc4.1 / ±0.11b6.5 / 35
14.8±0.01de / 4.2±0.05cd / 6.9±0.24ab / 21.2±0.04c / 3.4±0.03c / 6.6±0.03b / 42
±0.05e20.0 / ±0.11cd3.6 / ±0.25ab7.1 / ±0.02cd14.1 / ±0.02cd4.4 / ±0.10ab6.7 / 49
13.6±0.01e / 4.5±0.01cd / 7.0±0.30ab / 19.2±0.07cd / 3.6±0.05cd / 6.9±0.23ab / 56
±0.05ef18.4 / ±0.10cd3.6 / ±0.25a7.5 / ±0.05d13.0 / ±0.01d4.7 / ±0.17c7.3 / 63
12.3±0.08f / 4.6±0.00d / 7.8±0.15a / 18.0±0.04d / 3.7±0.03d / 7.5±0.10c / 70
41654.9 / 67.78 / 11.94 / 22737.32 / 359.43 / 9.78 / F value
0.0001 / 0.0001 / 0.0003 / 0.0001 / 0.0001 / 0.0006 / P value
Values are the mean of three replicates ± SD.
Values with the same letter in the same column are insignificant (p≤0.05)
*dSm-1 (decisiemens per metre) and l/KgVS (liter per kilogram volatile solids)