Course on Molecular Diagnostics (XI)
Thursday 2Friday 3 November,2017
vs 171024’
The ErasmusMedicalCenter houses many different molecular diagnostic techniques, which are subject to continuous research and are developed based on their practical applications. These applications are based on all areas of the healthcare, such as Clinical Genetics, Infectious Diseases, Complex Diseases and a broad spectrum of oncological adaptations.
This course will bring the state-of-the-art of these areas of the Molecular Diagnostics, and is meant for translational researchers as well as doctors and medical specialists operating outside of their initial field of research. But also PhD-students and technicians are welcome. This course is a yearly updatedfrom 2007 on. It will be given in English.
The course starts with 3 presentations on basic molecular techniques:
- PCR and real-time PCR;
- DNA sequencing; basic technologies;
- Next generation Sequencing (NGS);
followed by 1,5 day of applications in various fields
The course will take place in room Ae-3.27.Full participation will be awarded with 1 ECTS.For more information: The maximum amount of participants is 30. If you have any questions, please call Frank van Vliet (010-408 7518 or 06-5474 6408).
Organising committee:
Dr. Erik-Jan Dubbink, dept. of Pathology
Dr. Wilfred van IJcken, Biomics Core Facility
Dr. Corné Klaassen, Dept. of Microbiology
Dr. Ton Langerak, Dept. of Immunology
Dr. Ans van den Ouweland, Dept. of Clinical Genetics
Dr. Suzan Pas, Dept. of Viroscience
Dr. Peter Valk, Dept. of Hematology
Dr. Frank van Vliet, man. director Postgraduate School MolMed
Thursday 2 November2017, room Ae-3.27
Morning session: Techniques
Moderator: Wilfred van IJcken, Biomics Core Facility, Erasmus MC
Time / Speaker / Title / Keywords09.00-9.30 / Registration & coffee in room Ae-406
09.30-9.45 / Suzan Pas, Dept. of Viroscience, Erasmus MC / Opening, introduction
09.45-10.30 / Suzan Pas, Dept. of Viroscience, Erasmus MC / PCR, real-time PCR and new techno-logies / Both PCR and real-time PCR; dual probe/ dual target; Taqman-techniques; MGB & LNA probes; new technologies like Digital PCR, etc.
10.30-11.00 / Frank Sleutels,Dept. of Clinical Genetics, Erasmus MC / DNA sequencing; basic technologies / Sanger dideoxy, pyrosequencing; ‘when to use what’
11.00-11.15 / Coffee break
11.15-12.00 / Wilfred van IJcken, Biomics Core Facility / Next generation Sequencing (NGS) / High Throughput Sequencing, DNA & RNA Sequencing; overview of techniques, 1st/ 2nd/ 3rd generation techniques
12.00-12.15 / Wilfred van IJcken(chair), Suzan Pas, Frank Sleutels / Q&A with the audience; all questions will be answered! / ‘There are no stupid questions, only stupid answers’
12.15-13.00 /
Lunch break
Afternoon session: ApplicationsModerator: Corné Klaassen, Dept. of Microbiology
13.00-13.40 / Femke de Vries,Dept. of Clinical Genetics, Erasmus MC / Molecular (cyto) genetics and diagnostics of congenital defects / Karyotyping,FISH ,whole genome analysis,copy number variation13.40-14.20 / Suzan Pas, Dept. of Virology, Erasmus MC / Application of molecular techniques in virus diagnostics / Patient management; viral pathogenesis; quantitative (RT-)PCR; virus typing; Point of impact testing
14.20-14.35 / Coffee break
14.35-15.15 / Joyce van Meurs, Dept. of Internal Medicine, Erasmus MC / High throughput genotyping techniques / DNA variants, population genetics, array- and sequencing technology
15.15-15.20 / Short break / 15.45-16.00 / Coffee break
15.20-16.00 / Corné Klaassen, Dept. of Microbiology, Erasmus MC / Molecular microbio-logy: evolution of diagnostics and forensic microbiology / Real-time PCR, AFLP, MLVA, MLST, WGS, MS
16.00-16.40 / Ron van Schaik, Dept. of Clinical Chemistry, Erasmus MC / Pharmacogenetics of CYP450 enzymes / Pharmacogenetics: cytochrome P450, SNPs, personalized medicine
Friday 3 November2017, room Ae-3.27
Morning session: Applications (continued)
Moderator: Peter Valk, Dept. of Hematology, Erasmus MC
Time / Speaker / Title / Keywords09.00-09.10 /
Coffee
09.10-09.50 / Peter Valk, Dept. of Hematology, Erasmus MC / Genome-wide gene expression analyses in AML / High throughput gene expression arrays - Acute Myeloid Leukemia (AML)09.50-10.30 / Winand Dinjens, Dept. of Pathology, Erasmus MC / Pathology of solid tumors / Molecular diagnostics of solid tumors
10.30-10.45 /
Coffee break
10.45-11.25 / Ton Langerak, Dept. of Immunology, Erasmus MC / Molecular diagnostics of lymphoid malignancies / Clonality, IGHV SHM mutation, MRD, PCR fragment analysis, ASO-RQ-PCR, sequencing, NGS11.25-12.05 / Wim Dik, Dept. of Immunology, Erasmus MC / HLA typing and screening for clinical transplantation purposes / HLA, gene polymorphism, transplantation, DNA typing, PCR, dotblot, sequencing
12.05-12.45 / John Martens, Dept. of Medical Oncology, Erasmus / Liquid biopsies as prognostic and predictive biomarkers; ready for the clinic? / Liquid biopsies, biomarkers
12.45-13.30 / Lunch break
Afternoon session: Applications
Moderator: Ton Langerak, Dept. of Immunology, Erasmus MC
13.30-14.10 / David van de Vijver & Stefanie Popping, dept. of Viroscience, Erasmus MC / Cost-effectiveness in infectious diseases: prevention and diagnostics / Cost-effectiveness, PCR-testing, early identification, HCV, cost-effectiveness, MSM, PrEP14.10-14.50 / Ans van den Ouweland, Dept. of Clinical Genetics, Erasmus MC / Sequence change: pathogenic or just a neutral variant? / Classification decision tree
14.50-15.05 / Coffee break
15.05-15.45 / Miranda de Graaf, Dept. of Virology, Erasmus MC / Phylogeny in infectious diseases / An introduction to phylogeny. Evolution of RNA and DNA viruses
15.45-16.25 / Nico Hartwig, SFG / Casuistics: clinical cases / Genotyping, outbreaks, molecular epidemiology, patient care, infection prevention
Attendance fees
The subscription fee of non-commercial participants for the Course is €400.
Discounts are handled as followed:
- All PhD students and Master of Science students get 50% discount and pay €200
- All participants from the postgraduate schools MolMed and MGC get a discount of 100% and pay €0
- Master of Science students who pay private get a discount of 75% and pay €100.
If these financial requirements pose a problem but you wish to attend the Course, please contact Frank van Vliet, managing director of the Erasmus Postgraduate School Mol Med, at: .
Invoice
Fees can be paid upon an INVOICE. After your registration you will receive the INVOICE per mail. This can take a while. Payment per bank can be done together with the number on your INVOICE.
Late participants can also pay in cash upon signing in for the Course.
Cancellations
The fees are for all the days of the Course. There is no discount for partaking only in part of the Course. Our cancellation policy is that cancellation is possible up to one week before the start of the Course. Later cancellation will not be accepted, but you are allowed to send a substitute.
Commercial participants & sponsors
Companies are invited to inquire for participation and sponsoring.
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