SupplementaryTable 1: Plasmids.
Plasmid Description Source or
reference
pBBR1MCS-2Kmr, lacZα(Kovach et al., 1995)
pAS50pBBR1MCS-2 with wild-type (wt)mamJ(encoding J426) ofThis work
Magnetospirillum gryphiswaldense strain MSR-1inserted
between EcoRI-XbaI restriction sites
pAS51pBBR1MCS-2 with mamJof Magnetospirillum strain CF-2This work
inserted between EcoRI-XbaI restriction sites
pAS53pBBR1MCS-2 with mamJ of Magnetospirillum strain CF-3This work
inserted between EcoRI-XbaI restriction sites
pAS55pBBR1MCS-2 with mamJ of M. magnetotacticumstrain MS-1This work
inserted between EcoRI-XbaI restriction sites
pAS87pBBR1MCS-2 with mamJ constructJΔ81-256 inserted betweenThis work
EcoRI-XbaI restriction sites
pAS88pBBR1MCS-2 with mamJ constructJΔ136-294 inserted betweenThis work
EcoRI-XbaI restriction sites
pAS90pBBR1MCS-2 with mamJ constructJ1-386 inserted betweenThis work
EcoRI-XbaI restriction sites
pAS91pBBR1MCS-2 with mamJ constructJ1-392 inserted betweenThis work
EcoRI-XbaI restriction sites
pAS92pBBR1MCS-2 with mamJ constructJΔ1-24 inserted betweenThis work
EcoRI-XbaI restriction sites
pAS93pBBR1MCS-2 with mamJ construct JΔ1-45 inserted betweenThis work
EcoRI-XbaI restriction sites
pAS94pBBR1MCS-2 with mamJ construct JΔ293-334 inserted betweenThis work
EcoRI-XbaI restriction sites
pAS95pBBR1MCS-2 with mamJconstructJΔ335-360 inserted betweenThis work
EcoRI-XbaI restriction sites
pASG5pBBR1MCS-2 with mamK-egfp inserted between This work
EcoRI-XbaI restriction sites
pBTCmr, Two-hybrid bait fusion expression vectorStratagene
pBT-LGF2Cmr, Two-hybrid control plasmidStratagene
pBT_MamJpBT with wt mamJof strain MSR-1inserted betweenEcoRI-XhoIThis work
restriction sites
pBT_MamKpBT with mamKof strain MSR-1inserted between EcoRI-XhoIThis work
restriction sites
pBT_J1-135pBT with mamJ construct J1-135 inserted between EcoRI-XhoIThis work
restriction sites
pTRGTcr, Two-hybrid target fusion expression vectorStratagene
pTRG-Gal11PTcr, Two-hybrid control plasmidStratagene
pTRG_MamJpTRG with wt mamJ of strain MSR-1inserted between EcoRI-XhoI This work
restriction sites
pTRG_MamKpTRG with mamK of strain MSR-1inserted between EcoRI-XhoIThis work
restriction sites
pTRG_MamApTRG with wt mamAof strain MSR-1inserted between EcoRI-SpeI This work
restriction sites
pTRG_MamEpTRG with wt mamE of strain MSR-1inserted between EcoRI-XhoI This work
restriction sites
pTRG_MamPpTRG with wt mamP of strain MSR-1inserted between EcoRI-XhoIThis work
restriction sites
pTRG_J1-135pTRG withmamJconstruct J1-135inserted between EcoRI-XhoIThis work
restriction sites
pTRG_J81-256pTRG withmamJconstruct J81-256 inserted between EcoRI-XhoIThis work
restriction sites
pTRG_J136-294pTRG with mamJ construct J136-294 inserted between EcoRI-XhoIThis work
restriction sites
pTRG_J295-334pTRG with mamJ construct J295-334inserted between EcoRI-XhoIThis work
restriction sites
pTRG_J330-368pTRG with mamJ construct J330-368inserted between EcoRI-XhoIThis work
restriction sites
pTRG_J361-399pTRG with mamJconstruct J361-399 inserted between EcoRI-XhoIThis work
restriction sites
SupplementaryTable2: Primer.
NameTarget/PositionaSequence (5’ - 3’)
ASmamJs_f mamJb/1-19GAATTCATGGCAAAAAACCGGCGTG
ASmamJe_r1 mamJb/1251-1281TCTAGACTATTTATTCTTATCTTCAGCATCACATTTC
ASmamJ-34AS_r mamJb/1157-1176TCTAGACTAAACGAGGCGGCCTCCCTTGA
ASmamJ-40AS_r mamJb/1139-1158TCTAGACTAGACCACTCCATCGACGAATC
ASmamJ-N24AS_f mamJb/73-90GAATTCATGGAGATTGTTTCGGTGACG
ASmamJ-N45AS_f mamJb/136-154GAATTCATGGGCGACATTTGGGCTAGCT
ASmamJ-ala_f mamJb/1003-1017TTCGAGTATTCGCAGACCCGCCAGCCTAAC
ASmamJ-ala_r mamJb/862-876GTTAGGCTGGCGGGTCTGCGAATACTCGAA
ASmamJ-lag_f mamJb/1081-1095GTGGCGGTCGAAGCCGGGGGGATTTTCGGC
ASmamJ-lag_r mamJb/988-1002GCCGAAAATCCCCCCGGCTTCGACCGCCAC
ASmamJ-otr_r mamJb/223-240AACCGGCGCGGGGTCCTGGGAACGAATGGG
ASmamJ-otr_f mamJb/769-780CCCATTCGTTCCCAGGACCCCGCGCCGGTT
ASmamJ-oad_r mamJb/388-405CAAATCCGCTGATGCAACGCTCGGCTGGACATCAATCAC
ASmamJ-oad_f mamJb/886-903GTGATTGATGTCCAGCCGAGCGTTGCATCAGCGGATTTG
ASmamJ-MS1_f mamJc/1-32GAATTCATGGCGAATAACCGGCGAGATCGCGACAAAGG
ASmamJ-MS1_r mamJc/1147-1173TCTAGACTATTTTTTCTTGCCCACCGTATCGCA
ASmamKs_f mamKb/4-18GAATTCGGAGGTCTTCATATGTGGATTGATCTGTTA
ASmamKe_r4 mamKb/1063-1080GATCGAGCCCTGACCGGAAACGTCACC
ASegfp_f11 egfpd/4-21TCCGGTCAGGGCTCGATCGTGAGCAAGGGCGAGGAG
ASegfp_r3 egfpd/697-720TCTAGATTACTTGTACAGCTCGTCCATGCC
ASmamJ_pBT_f mamJb/1-19GAATTCGATGGCAAAAAACCGGCGTG
ASmamJ_pTRG_f mamJb/1-19GAATTCAGATGGCAAAAAACCGGCGTG
ASmamJe_r9 mamJb/1251-1281CTCGAGCTATTTATTCTTATCTTCAGCATCACATTTC
ASmamK_pBT_f mamKb/1-21GAATTCCCTGTGGATTGATCTGTTAGCA
ASmamK_pTRG_f mamKb/1-21GAATTCTGTGGATTGATCTGTTAGCA
ASmamK_pTRG_r2 mamKb/1058-1082CTCGAGTCACTGACCGGAAACGTCACCAAGCT
ASmamA_pTRG_f mamAb/1-16GAATTCAGATGTCTAGCAAGCCGT
ASmamA_pTRG_r mamAb/640-654ACTAGTTTAGACGGCCGAACG
ASmamP_pTRG_f mamPb/1-16GAATTCAGATGAATAGCAAACTCG
ASmamP_pTRG_r mamPb/796-813CTCGAGCTAATTTATCACGTGGCA
ASmamE_pTRG_f mamEb/1-24GAATTCAGATGACCATGTTCAATGGTGATGTG
ASmamE_pTRG_r mamEb/2302-2319CTCGAGTCAAAGAACAATCCAGAA
ASglyTRG_f mamJb/1081-1095GAATTCAGGGGGGGATTTTCGGC
ASgly_r1 mamJb/1186-1197CTCGAGCTACCCGGCGACCAC
ASalaTRG_f mamJb/883-899GAATTCAGAGCGTTGCATCAGCGGA
ASala_r mamJb/988-1002CTCGAGCTAGGCTTCGACCGCCAC
ASlinkA-GTRG_f mamJb/988-1001GAATTCAGGTGGCGGTCGAAGC
ASlinkA-G_r mamJb/1094-1104CTCGAGCTATCCGGCGACGC
ASgluTRG_f mamJb/406-417GAATTCAGGAAACGGATGCC
ASglu_r mamJb/868-882CTCGAGCTACTCCGCCTGCGAATA
ASlarger_r mamJb/488-504CTCGAGCTATTCCGGCTCGACTTCGG
ASlargerTRG_f mamJb/241-255GAATTCAGCCTGTGCCGGTTGCC
ASN-termi_r mamJb/391-405CTCGAGCTACGGCTGGACATCAAT
Restriction sites are underlined. Additional nucleotides which do not bind to the template sequence during PCR.
aPosition within the target sequence.
bGene of Magnetospirillum gryphiswaldense strain MSR-1 (Sequence deposition number BX571797).
cGene of Magnetospirillum magnetotacticum strain MS-1(Sequence deposition number NZ_AAAP01003824.1).
dTargets the egfp gene from vector pEGFP-N3 (Sequence deposition number U57609).
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