Genotype / Oryzasativa L. var. Sabita / Oryzasativa L.
var. Swarna / Oryzasativa L.
var. Swarna -Sub1 / OryzaeichingeriA. Peter
Photosensitivity
Ecosystem
Water depth / Photosensitive
Semi deep/deep water
>75 cm / Weakly photosensitive
Rainfed shallowlowland
≥30 cm / Weakly photosensitive
Submergence tolerant
Not known / Not known
Wetland
Not known
N2 response / Non responsive / Responsive / Responsive / Not known
Growth durations (d) / ~155 / ~145 / ~145 / Perennial
Table S1 Rice genotypes and related genera used in the experiment.
Type and texture / pH / Eh (mS/cm) / Available p(kg/ha) / Available K (kg/ha) / Organic C (%) / Available N (%) / Available Ca (%) / Available Mg (%) / Available S (%) / Available iron (Fe2+) (mg/g soil)Alluvial sandy loam / 6.53±2.20 / 5.32±0.20 / 22.20±1.10 / 102.06±2.11 / 0.45±0.04 / 0.064±0.004 / 0.043±0.002 / 0.041±0.004 / 0.038±0.002 / 1.54±0.03
CD, P 0.05 / 0.04 / 0.04 / 1.34 / 4.09 / 0.6 / 0.009 / 0.007 / 0.004 / 0.002 / 0.001
Table S2Physical properties of soil of rice fields use for the plant sample collection. The fields had been treated with N-P-K (120:60:60) fertilizer
1
Table S3Morphological characters of phytonic diazotrophs
Rice genotype / Isolate no. / Form / Colour / Elevation / Margin / Size (mm) / ConsistencySabita / SA5 / Circular / Off white / Flat / Entire / 0.5 -0.8 / Mucoid
SA12 / Circular / Off white / Convex / Entire / 0.6 -0.8 / Gummy
SA2 / Circular / Off white / Convex / Entire / 0.4 -0.6 / Mucoid
SA4 / Circular / Off white / Convex / Undulate / 0.8 -0.9 / Gummy
SA6 / Circular / White / Convex / Entire / 0.3 -0.5 / Gummy
SA7 / Circular / White / Convex / Entire / 0.7 -0.9 / Gummy
SA10 / Circular / Off white / Convex / Entire / 0.7 -0.8 / Mucoid
Swarna / SW1 / Circular / White due drop / Flat / Entire / 0.4 -0.6 / Gummy
SW6B / Circular / Off white / Convex / Entire / 0.6 -0.8 / Gummy
SW9 / Circular / Off yellowish / Convex / Entire / 0.7 -0.8 / Gummy
SW10 / Circular / Off white / Convex / Entire / 0.7 -0.8 / Gummy
SW12 / Circular / Off white / Convex / Entire / 0.3 -0.5 / Gummy
SW13 / Circular / Off white / Convex / Entire / 0.7 -0.8 / Gummy
SW Azo / Circular / Brown / Convex / Entire / 0.5 -0.8 / Gummy
Avi2 / Circular / Brown / Convex / Entire / 0.4 -0.5 / Gummy
SBC14 / Circular / Off white / Convex / Entire / 0.4 -0.6 / Mucoid
Swarna- Sub1 / SB19 / Circular / Brown / Convex / Entire / 0.5 -0.6 / Gummy
SBUA / Circular / White / Convex / Entire / 0.4 -0.5 / Dry
SBUB / Circular / Entire / Off white / Convex / 0.7 -0.8 / Gummy
SB2 / Circular / White / Flat pinheaded / Entire / 0.4 -0.5 / Not gummy
SBC17 / Circular / Off white / Flat / Entire / 0.5 -0.8 / Gummy
SBC1a / Circular / Orange / Convex / Entire / 0.4 -0.6 / Gummy
SBC38 / Small Circular / White / Flat / Entire / 0.5 -0.8 / Mucoid
SBC3 / Circular / Off white / Convex / Entire / 0.6 -0.7 / Gummy
Wild / WR8 / Circular / Off white / Flat / Entire / 0.5 - 0.6 / Not gummy
WR15 / Circular / Brown / Convex / Entire / 0.6 - 0.7 / Gummy
WR22 / Circular / Brown / Flat / Entire / 0.5 -0.6 / Gummy
WR23 / Circular / White / Convex / Entire / 0.4 -0.5 / Mucoid
WR9 / Circular / White due drop / Convex / Entire / 0.97 -1.2 / Mucoid
WR13A / Circular / White / Convex / Entire / 0.8 -0.9 / Gummy
WR13B / Circular / Off white / Flat / Entire / 0.4 - 0.5 / Not gummy
WR21 / Circular / White / Convex / Entire / 0.7 -0.8 / Partly gummy
WR2 / Circular / White / Convex / Entire / 0.95 -1.00 / Gummy
WR18 / Circular / Off white / Convex / Entire / 0.6 -0.8 / Gummy
WR17 / Circular / Black / Umbonate / Undulate / 0.5 - 0.6 / Not gummy
1
TableS4 Biochemical and physiological characters of phytonic diazotrophs (+ positive, - negative)
Isolate no. / Indole Test / Methyl red / Voges-Proskaure / Citrate Utilaztion / Nitrate reduction / Urease / Oxidase / Catalase / Cell morphology / Gram stain / Spore / Motility / CystSabita / SA5 / - / - / + / + / - / - / + / + / Rod / + / + / + / -
SA12 / - / - / - / + / - / - / + / + / Rod / - / - / + / -
SA2 / + / - / - / + / - / - / + / + / Rod / - / - / + / -
SA4 / - / - / - / + / - / - / + / + / Rod / - / - / + / -
SA6 / + / - / + / + / - / - / + / + / Rod / + / - / + / -
SA7 / - / - / - / - / - / - / + / + / Rod / - / - / + / -
SA10 / + / - / - / - / - / - / + / + / Rod / - / - / + / -
Swarna / SW1 / + / - / + / + / - / + / + / + / Rod / - / - / - / -
SW6B / + / - / + / + / - / - / + / + / Rod / + / + / + / -
SW9 / + / - / - / - / + / - / + / + / Rod / - / - / - / -
SW10 / - / - / - / + / - / - / + / + / Rod / - / - / + / -
SW12 / - / - / + / + / + / + / + / + / Rod / - / - / - / -
SW13 / - / - / - / + / + / + / + / + / Rod / - / - / - / -
SW Azo / + / + / + / + / - / - / + / + / Rod / + / + / + / -
Avi2 / + / + / + / + / + / + / + / + / Rod / - / - / + / +
SBC14 / + / + / + / + / + / + / + / + / Rod / - / - / + / -
Swarna-Sub1 / SB2 / + / - / - / + / - / - / + / + / Rod / - / - / - / -
SBC3 / + / + / + / + / + / + / + / + / Rod / - / - / + / -
SB19 / - / + / + / + / - / - / + / + / Rod / - / - / - / -
SBUA / + / - / - / + / + / - / + / + / Rod / - / + / - / -
SBUB / + / - / - / + / + / - / + / + / Rod / - / - / - / -
SBC17 / - / + / + / + / + / + / + / + / Rod / - / - / + / -
SBC1a / + / + / + / + / - / - / + / + / Rod / + / - / + / -
SBC38 / + / + / + / + / + / + / + / + / Rod / - / - / -
Wild / WR2 / - / - / - / - / - / - / + / + / Rod / - / - / + / -
WR8 / + / - / + / + / - / + / + / + / Rod / - / - / - / -
WR9 / + / - / - / - / - / - / + / + / Rod / - / - / + / -
WR13A / + / - / + / + / - / + / + / + / Rod / - / - / - / -
WR13B / - / + / - / + / - / + / + / + / Rod / + / - / + / -
WR15 / + / - / + / + / - / + / + / + / Rod / - / - / - / -
WR17 / + / - / - / + / + / - / + / + / Rod / - / - / - / -
WR18 / - / - / - / + / + / - / + / + / Rod / - / - / - / -
WR21 / + / - / - / - / - / - / + / + / Rod / - / - / + / -
WR22 / + / - / + / + / - / + / + / + / Rod / - / - / - / -
WR23 / + / + / + / + / + / - / + / + / Rod / - / - / - / -
1
Table S5 Sugar utilization patterns of all the isolates, isolated from four rice genotypes (+ positive, - negative, W+ - weakly positive)
Rice genotypes / Isolate no. / Lactose / Xylose / Maltose / Fructose / Dextrose / Galactose / Raffinose / Trehalose / Melibiose / Sucrose / L- arabinose / Mannose / Inulin / Sodium Gluconate / Glycerol / Salicin / Dulcitol / InositolSabita / SA5 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SA12 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SA2 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SA4 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SA6 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SA7 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SA10 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
Swarna / SW1 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SW6B / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / - / W+
SW9 / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / - / W+
SW10 / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / - / W+
SW12 / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / - / W+
SW13 / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / - / W+
SW Azo / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / - / W+
Avi2 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SBC14 / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / - / W+
Swarna- Sub1 / SB19 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SBUA / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SBUB / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SB2 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SBC17 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SBC1a / + / + / + / + / + / + / + / + / + / + / + / + / + / W+ / + / + / - / W+
SBC38 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
SBC3 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
Wild / WR8 / + / W+ / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
WR15 / + / + / + / W+ / + / + / - / + / + / + / - / - / - / - / W+ / W+ / - / -
WR22 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / + / +
WR23 / + / + / + / W+ / + / + / - / - / - / - / - / + / - / - / W+ / W+ / W+ / W+
WR9 / + / + / + / W+ / + / + / - / + / + / + / - / - / - / - / W+ / W+ / - / -
WR13A / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
WR13B / + / + / + / + / + / + / +GAS / GAS+ / GAS+ / GAS+ / GAS+ / - / + / - / + / + / - / -
WR21 / + / + / + / W+ / + / + / - / - / - / - / - / + / - / - / W+ / W+ / W+ / W+
WR2 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / - / W+
WR18 / + / + / + / W+ / + / + / - / - / - / - / - / + / - / - / W+ / W+ / W+ / W+
WR17 / + / + / + / + / + / + / + / + / + / + / + / + / + / - / + / + / W+ / W+
1
Table S6 Effect of inoculation of thirty-five N2-fixing, plant growth promoting endophytic and epiphytic isolates on four growth parameters ( shoot length, root length, fresh weight, dry weight) of rice (cv. Swarna) after 12 d of seedling bacterization
Name of genotype / Isolate / Root length (cm) / Shoot length(cm) / Fresh weight(g) / Dry weight (g)Sabita / SA5 / 4.27O / 3.60KLM / 0.09CDE / 0.01CD
SA12 / 4.37MNO / 3.87IJKLM / 0.08DE / 0.01BCD
SA2 / 4.20O / 3.83IJKLM / 0.08DE / 0.01CD
SA4 / 4.93IJKLMN / 3.87IJKLM / 0.08DE / 0.01CD
SA6 / 4.30NO / 3.77JKLM / 0.09BCDE / 0.01CD
SA7 / 4.33NO / 3.53LM / 0.09BCDE / 0.01CD
SA10 / 4.53JKLMNO / 3.57KLM / 0.09BCDE / 0.01D
Swarna / SW1 / 5.30CDEFGHI / 4.40DEFGH / 0.10BCDE / 0.01D
SW6B / 5.33CDEFGHI / 4.37DEFGH / 0.09CDE / 0.01D
SW9 / 6.30AB / 4.37DEFGH / 0.09CDE / 0.01ABCD
SW10 / 4.93IJKLMN / 4.30EFGHI / 0.09CDE / 0.01CD
SW12 / 5.17EFGHIJ / 5.27AB / 0.09CDE / 0.01D
SW13 / 5.77BCDEF / 4.76CDE / 0.12AB / 0.01BCD
SW Azo / 5.20DEFGHI / 4.47CDEFGH / 0.09BCDE / 0.01CD
Avi2 / 6.83A / 5.73A / 0.15A / 0.02A
SBC14 / 5.56CDE / 3.80JKLM / 0.09BCDE / 0.01CD
Swarna-Sub1 / SB19 / 5.07HIJKL / 4.90BC / 0.09BCDE / 0.01CD
SBUA / 5.83BCD / 4.50CDEFGH / 0.07DE / 0.01D
SBUB / 5.87BC / 4.53CDEFG / 0.08CDE / 0.01D
SB2 / 5.83BCD / 4.10GHIJKL / 0.07DE / 0.01BCD
SBC3 / 4.10OP / 4.57CDEFG / 0.09CDE / 0.01BCD
SBC1a / 6.30AB / 5.26AB / 0.12AB / 0.01ABCD
SBC38 / 5.00IJKLM / 4.1GHIJKL / 0.08DE / 0.01CD
SBC17 / 5.37CDEFGHI / 4.43DEFG / 0.08CDE / 0.01D
Wild / WR8 / 5.23CDEFGHI / 4.10GHIJKL / 0.08CDE / 0.01CD
WR15 / 4.30NO / 4.23EFGHIJ / 0.09BCDE / 0.01CD
WR22 / 4.43LMNO / 4.20EFGHIJ / 0.10BCD / 0.01CD
WR23 / 4.47KLMNO / 4.73CDE / 0.11BC / 0.01CD
WR9 / 5.77BCDEF / 4.70CDEF / 0.10BCD / 0.01BCD
WR13A / 5.13FGHIJ / 4.10GHIJKL / 0.10BCDE / 0.01CD
WR13B / 5.73BCDEFG / 4.83BCD / 0.08DE / 0.01CD
WR21 / 5.10GHIJK / 4.10GHIJKL / 0.10BCDE / 0.01CD
WR2 / 5.80BCDE / 4.76CDE / 0.08DE / 0.01CD
WR18 / 5.17EFGHIJ / 3.93HIJKLM / 0.07DE / 0.01CD
WR17 / 5.70BCDEFGH / 4.13FGHIJK / 0.08CDE / 0.01CD
Control / 3.50P / 3.50M / 0.07E / 0.01CD
CV(%) / 3.44 / 3.65 / 9.2 / 8.28
SE(d) / 0.144 / 0.123 / 0.007
Tukey HSD at 1% / 0.6501 / 0.5433 / 0.0308
1
Figure S1Representative PCR amplification of 16S rRNA genes
1
Figure S2PCR amplification of nifH genes of 11 isolates.
Isolate / Primer usedSB19 / pol f/ pol r
SW13 / pol f/ pol r
WR18 / nifh2/ r6
WR23 / f2/nifh1
SA12 / nifh2/ nifh1
SW12 / pol f/ pol r
SW1 / pol f/ pol r
SB2 / f2/nifh1
SBUA / f2/nifh1
WR8 / pol f/ pol r
Avi2 / pol f/ pol r
1
Fig. S3 Representative pictures of in-vitro plant growth promoting attributes of different isolates. A. ammonia production in peptone broth (C- control), B. nitrite production(C- control), C. indole production (Trp- tryptophan), D. siderophore production
1
Figure S4 Clustering based on BIOLOG based sugarutilizationand carbohydrate utilization patttern through KB009 HiCarbohydrateTM kit, Hi-MEDIA.
1