Figure S1 Phylogenetic Relationships and Subgroup Designationsin WDR Proteins in Cucumber

Figure S1Phylogenetic relationships and subgroup designationsin WDR proteins in cucumber andArabidopsis.

Figure S2Expression patterns of cucumber abiotic-responsiveWDR genes under different treatment conditions. A: NaCl(100mM); B: ABA (100 μM); C: Low temperature (4℃). The cucumber -actin gene (GenBank AB010922) was performed as an internalcontrol. The PCR primers were designed to avoid the conservedregion and to amplify products of 150 to 300 bp. Primer sequenceswere shown in detail in Table S3.

Figure S3Tissue-specific expression profiles of 6 cucumberWDR genes. Total RNA was isolated from roots (R), stems (S), leaves (L), male flowers (MF), fruits (F) and tendrils (T). The cucumber -actin gene (GenBank AB010922) was used to adjust cDNA concentrations. The PCR primers were designed to avoid the conserved region and to amplify products of 150 to 300 bp. Primer sequences were shown in detail in Table S3.

Table S1 A catalog of WDR proteins in cucumber

Gene / Location / Number of WDR / Deduced polypeptide
Length(aa) / PI / MW(KDa)
Csa4M097650 / Chr4:6379877- 6381345 / 2 / 333 / 5.12 / 37.19
Csa3M253490 / Chr3:15736092-15737760 / 2 / 346 / 4.65 / 38.88
Csa1M587430 / Chr1:22068843-22072681 / 3 / 475 / 5.07 / 52.11
Csa6M519610 / Chr6:27623629-27624918 / 5 / 316 / 5.45 / 35.48
Csa7M012400 / Chr7:743011-745491 / 5 / 393 / 4.62 / 44.04
Csa2M047770 / Chr2:3921295-3928209 / 3 / 490 / 4.52 / 53.74
Csa5M141120 / Chr5:3923102-3925548 / 4 / 455 / 7.75 / 50.49
Csa3M685700 / Chr3:26229734-26241314 / 2 / 1112 / 5.16 / 120.58
Csa3M702570 / Chr3:26636299-26640162 / 4 / 453 / 7.74 / 50.38
Csa5M517720 / Chr5:18155342-18157956 / 4 / 475 / 9.23 / 51.95
Csa5M431540 / Chr5:15690261-15706356 / 7 / 541 / 9.07 / 60.90
Csa5M156190 / Chr5:5468015-5471856 / 5 / 465 / 9.34 / 51.34
Csa4M637680 / Chr4:20751673-20758425 / 3 / 854 / 6.36 / 95.90
Csa6M492270 / Chr6:23712440-23724383 / 5 / 283 / 7.58 / 31.91
Csa3M166250 / Chr3:11009142-11018918 / 6 / 1781 / 6.26 / 199.95
Csa7M452950 / Chr7:19130008-19131592 / 5 / 301 / 5.62 / 32.62
Csa4M187290 / Chr4:9098972-9104661 / 7 / 434 / 5.77 / 48.29
Csa6M497140 / Chr6:24363655-24365671 / 7 / 318 / 6.44 / 34.93
Csa3M462670 / Chr3:20804345-20807395 / 6 / 301 / 5.76 / 32.66
Csa6M404170 / Chr6:18172021-18176011 / 7 / 569 / 5.30 / 63.05
Csa1M588520 / Chr1:22182283-22190611 / 4 / 529 / 6.04 / 58.73
Csa7M388400 / Chr7:14417430-14420312 / 7 / 313 / 8.75 / 34.48
Csa5M580650 / Chr5:20576959-20586168 / 5 / 967 / 7.79 / 106.38
Csa3M061010 / Chr3:3521704-3524918 / 4 / 318 / 6.30 / 35.66
Csa1M590260 / Chr1:22403915-22408454 / 7 / 321 / 5.79 / 34.50
Csa3M687770 / Chr3:26306563-26317320 / 8 / 506 / 7.27 / 56.11
Csa1M699620 / Chr1:28132617-28144293 / 3 / 709 / 6.59 / 79.48
Csa6M483500 / Chr6:22312329-22316056 / 3 / 668 / 6.07 / 75.47
Csa2M379340 / Chr2:19323127-19331187 / 5 / 894 / 5.84 / 97.90
Csa4M652030 / Chr4:22609201-22617204 / 6 / 703 / 6.28 / 78.05
Csa3M516570 / Chr3:21499758-21512738 / 4 / 958 / 5.12 / 108.50
Csa1M537520 / Chr1:19563108-19574550 / 6 / 727 / 8.28 / 80.73
Csa1M072490 / Chr1:7334960-7343561 / 5 / 636 / 6.49 / 69.77
Csa6M358120 / Chr6:16053353-16062762 / 1 / 357 / 5.10 / 39.93
Csa1M403660 / Chr1:14819483-14827734 / 5 / 828 / 7.58 / 91.71
Csa4M004810 / Chr4:760712-765955 / 5 / 892 / 6.29 / 99.88
Csa6M319790 / Chr6:15089031-15094527 / 6 / 476 / 9.31 / 52.51
Csa3M131970 / Chr3:8553604-8562095 / 5 / 767 / 6.17 / 83.37
Csa6M152320 / Chr6:10773883-10781387 / 6 / 1033 / 7.57 / 113.36
Csa3M611350 / Chr3:23760522-23765918 / 4 / 646 / 5.84 / 71.47
Csa5M588760 / Chr5:21080158-21082622 / 7 / 344 / 6.59 / 38.12
Csa7M390220 / Chr7:14663518-14665802 / 2 / 528 / 9.58 / 58.60
Csa7M004160 / Chr7:212172-218476 / 5 / 1217 / 6.64 / 136.28
Csa2M249910 / Chr2:12278556-12283698 / 5 / 691 / 9.03 / 78.92
Csa1M002820 / Chr1:502969-505746 / 6 / 886 / 8.56 / 99.54
Csa3M134680 / Chr3:8951448-8960583 / 5 / 522 / 5.84 / 57.00
Csa1M267200 / Chr1:12928207-12932039 / 2 / 548 / 6.45 / 61.04
Csa3M839810 / Chr3:33671103-33675854 / 4 / 515 / 8.42 / 57.23
Csa3M914590 / Chr3:39700369-39701971 / 3 / 383 / 5.87 / 41.34
Csa1M024830 / Chr1:2554015-2556107 / 7 / 327 / 6.71 / 36.03
Csa5M150990 / Chr5:4597958-4604450 / 3 / 1052 / 5.85 / 117.14
Csa5M649250 / Chr5:27561072-27568129 / 4 / 344 / 8.50 / 38.15
Csa3M829070 / Chr3:33097623-33099593 / 5 / 301 / 6.03 / 32.55
Csa6M486680 / Chr6:22535912-22542877 / 5 / 299 / 6.82 / 33.09
Csa7M038680 / Chr7:2074628-2079705 / 5 / 451 / 9.61 / 51.72
Csa5M601580 / Chr5:22038324-22041655 / 4 / 581 / 6.46 / 65.89
Csa4M614220 / Chr4:19483860-19489614 / 5 / 584 / 6.36 / 65.00
Csa6M016980 / Chr6:1955275-1968517 / 4 / 236 / 4.20 / 25.43
Csa5M148670 / Chr5:4354184-4359375 / 3 / 743 / 5.90 / 83.34
Csa4M015820 / Chr4:2089587-2094822 / 1 / 455 / 5.30 / 51.64
Csa3M811590 / Chr3:31134695-31138623 / 5 / 316 / 6.16 / 35.38
Csa1M042240 / Chr1:4174401-4186880 / 6 / 1220 / 5.81 / 135.00
Csa7M267960 / Chr7:9478314-9482411 / 7 / 377 / 7.95 / 40.83
Csa1M024940 / Chr1:2615121-2620964 / 7 / 557 / 5.83 / 59.94
Csa1M418250 / Chr1:15133477-15138764 / 4 / 353 / 5.43 / 38.59
Csa6M185330 / Chr6:12024850-12030715 / 2 / 562 / 6.63 / 63.55
Csa3M088960 / Chr3:4662553-4664475 / 5 / 303 / 8.50 / 33.53
Csa2M354740 / Chr2:16526749-16532001 / 4 / 446 / 8.80 / 49.02
Csa5M139180 / Chr5:3428961-3434342 / 4 / 720 / 7.50 / 80.67
Csa4M295520 / Chr4:11873998-11878576 / 4 / 946 / 8.04 / 105.15
Csa1M045830 / Chr1:5154911-5156629 / 7 / 383 / 8.25 / 42.59
Csa2M308350 / Chr2:14703231-14707651 / 2 / 451 / 5.29 / 51.06
Csa4M614210 / Chr4:19472689-19477443 / 5 / 591 / 5.65 / 65.94
Csa5M593390 / Chr5:21508034-21509505 / 6 / 445 / 9.55 / 48.43
Csa3M360140 / Chr3:18420473-18428873 / 3 / 584 / 5.59 / 64.44
Csa4M307950 / Chr4:12560983-12571470 / 4 / 1237 / 6.21 / 135.34
Csa1M004880 / Chr1:848104-850314 / 2 / 346 / 5.40 / 37.96
Csa2M249890 / Chr2:12261378-12271432 / 2 / 508 / 8.87 / 56.80
Csa2M292220 / Chr2:13756726-13758141 / 6 / 471 / 8.62 / 51.79
Csa4M638360 / Chr4:20864115-20874013 / 4 / 938 / 5.99 / 103.52
Csa3M464690 / Chr3:20860632-20863096 / 5 / 445 / 8.32 / 49.91
Csa3M055980 / Chr3:3484956-3490797 / 1 / 454 / 5.00 / 51.21
Csa5M647380 / Chr5:27342269-27348849 / 6 / 502 / 5.72 / 56.48
Csa3M842110 / Chr3:33932135-33938333 / 3 / 451 / 8.10 / 49.05
Csa6M008800 / Chr6:931925-937451 / 6 / 799 / 6.31 / 86.79
Csa7M075570 / Chr7:5219417-5221137 / 6 / 467 / 9.24 / 51.84
Csa4M325540 / Chr4:13201028-13216720 / 2 / 1362 / 5.97 / 149.93
Csa2M169190 / Chr2:9482623-9491863 / 2 / 775 / 6.18 / 85.12
Csa4M378260 / Chr4:15163545-15167119 / 1 / 396 / 4.61 / 44.43
Csa3M890030 / Chr3:37964780-37978058 / 4 / 749 / 6.02 / 82.68
Csa2M070230 / Chr2:5356348-5372042 / 5 / 891 / 6.79 / 99.87
Csa2M012680 / Chr2:2213179-2226936 / 6 / 757 / 6.46 / 83.03
Csa4M001690 / Chr4:370349-375042 / 2 / 424 / 4.92 / 46.62
Csa1M015060 / Chr1:1983814-1988995 / 3 / 962 / 6.08 / 105.82
Csa5M610440 / Chr5:23765080-23770760 / 8 / 840 / 6.38 / 92.17
Csa7M391250 / Chr7:14715648-14723981 / 3 / 497 / 6.96 / 55.53
Csa4M639920 / Chr4:21247342-21250977 / 3 / 447 / 6.60 / 49.11
Csa3M872720 / Chr3:36453662-36461055 / 1 / 1489 / 5.84 / 165.76
Csa2M385060 / Chr2:20113558-20118573 / 1 / 536 / 9.57 / 60.48
Csa1M000560 / Chr1:39583-43759 / 1 / 684 / 6.81 / 76.94
Csa6M524070 / Chr6:28202367-28205041 / 4 / 340 / 6.63 / 36.80
Csa1M039850 / Chr1:3919838-3925014 / 3 / 414 / 6.73 / 45.45
Csa2M033270 / Chr2:3163900-3172658 / 4 / 1127 / 6.99 / 124.76
Csa3M664570 / Chr3:25972727-25989572 / 4 / 1368 / 6.16 / 152.67
Csa6M314090 / Chr6:14914386-14924002 / 3 / 539 / 7.86 / 58.51
Csa1M002840 / Chr1:517062-518160 / 6 / 345 / 9.55 / 38.34
Csa3M854190 / Chr3:35567824-35569212 / 5 / 462 / 9.01 / 50.32
Csa2M385000 / Chr2:20094395-20098782 / 2 / 393 / 5.47 / 43.43
Csa1M696500 / Chr1:27966814-27973729 / 1 / 1329 / 5.22 / 147.84
Csa1M051780 / Chr1:6148539-6153887 / 1 / 524 / 9.37 / 58.10
Csa1M050390 / Chr1:5887225-5890016 / 3 / 473 / 8.92 / 52.14
Csa3M464700 / Chr3:20875287-20878864 / 4 / 450 / 8.46 / 48.97
Csa4M006320 / Chr4:1069354-1076696 / 3 / 1163 / 6.98 / 127.85
Csa1M467110 / Chr1:16720845-16725089 / 3 / 761 / 5.52 / 84.25
Csa5M647430 / Chr5:27367870-27373574 / 3 / 686 / 6.41 / 78.47
Csa1M470270 / Chr1:16904557-16911371 / 3 / 1365 / 6.75 / 152.12
Csa7M387800 / Chr7:14344969-14349987 / 3 / 377 / 5.71 / 40.97
Csa2M360750 / Chr2:17107733-17109933 / 6 / 406 / 8.59 / 44.44
Csa3M838780 / Chr3:33639646-33644441 / 3 / 337 / 6.17 / 37.35
Csa3M126150 / Chr3:7716164-7722645 / 2 / 424 / 8.69 / 46.19
Csa6M000660 / Chr6:118220-126835 / 1 / 1613 / 6.07 / 180.28
Csa6M422800 / Chr6:19544089-19554812 / 2 / 363 / 8.67 / 40.66
Csa4M000900 / Chr4:225818-229623 / 3 / 349 / 8.51 / 38.81
Csa2M368290 / Chr2:17769846-17778365 / 2 / 844 / 6.31 / 94.11
Csa1M589670 / Chr1:22328712-22336576 / 2 / 373 / 6.64 / 40.79
Csa5M440090 / Chr5:15879530-15880892 / 6 / 423 / 8.83 / 46.30
Csa5M612300 / Chr5:24014386-24017568 / 3 / 490 / 4.72 / 54.13
Csa4M083560 / Chr4:5643195-5644820 / 3 / 442 / 6.25 / 47.77
Csa4M269200 / Chr4:10663440-10665666 / 4 / 553 / 7.69 / 61.53
Csa3M775290 / Chr3:29693485-29702113 / 1 / 952 / 6.65 / 105.87
Csa3M124950 / Chr3:7547072-7570569 / 1 / 3025 / 5.39 / 330.94
Csa4M643110 / Chr4:21629752-21635488 / 3 / 818 / 6.26 / 90.48
Csa1M575030 / Chr1:21763097-21772762 / 2 / 1538 / 6.39 / 172.48
Csa4M051460 / Chr4:4232806-4237455 / 3 / 327 / 5.45 / 36.11
Csa4M050140 / Chr4:3959242-3970859 / 1 / 1498 / 5.82 / 164.84
Csa5M286090 / Chr5:11922226-11925866 / 1 / 303 / 5.07 / 32.76
Csa3M892720 / Chr3:38134038-38142867 / 2 / 3229 / 6.12 / 362.49
Csa1M071910 / Chr1:7269679-7273715 / 2 / 417 / 4.58 / 46.62
Csa7M379100 / Chr7:14068835-14090794 / 2 / 3606 / 5.82 / 402.14
Csa1M386570 / Chr1:14591069-14600761 / 1 / 465 / 6.16 / 51.72
Csa3M159430 / Chr3:10770646-10779005 / 1 / 1342 / 6.32 / 149.61
Csa1M050310 / Chr1:5854680-5858914 / 1 / 430 / 8.94 / 47.36
Csa1M153530 / Chr1:9959884-9970839 / 2 / 624 / 6.67 / 70.22
Csa7M395790 / Chr7:15169716-15172231 / 1 / 420 / 6.60 / 46.16
Csa5M645110 / Chr5:27169526-27173269 / 1 / 392 / 5.89 / 43.63
Csa1M572400 / Chr1:21271196-21276252 / 1 / 771 / 7.64 / 84.08
Csa7M391270 / Chr7:14732960-14740212 / 1 / 931 / 5.67 / 102.11
Csa3M415110 / Chr3:19748986-19753767 / 1 / 881 / 8.61 / 95.86
Csa3M045180 / Chr3:3196648-3207478 / 1 / 1132 / 5.13 / 122.47
Csa2M279240 / Chr2:13382550-13385921 / 2 / 564 / 6.31 / 62.78
Csa5M167100 / Chr5:6478720-6483444 / 1 / 450 / 5.41 / 50.96
Csa3M166260 / Chr3:11020718-11032015 / 5 / 671 / 8.52 / 74.94
Csa3M127190 / Chr3:8042734-8046100 / 5 / 423 / 4.71 / 48.28
Csa6M483440 / Chr6:22264573-22269712 / 6 / 480 / 5.94 / 52.59
Csa3M611390 / Chr3:23786903-23787897 / 4 / 201 / 7.01 / 22.37
Csa1M690190 / Chr1:27619750-27624930 / 5 / 383 / 6.46 / 41.95
Csa3M776910 / Chr3:29786782-29791607 / 7 / 350 / 4.88 / 38.98
Csa6M161200 / Chr6:11250850-11265620 / 7 / 675 / 6.65 / 76.14
Csa2M249920 / Chr2:12287853-12291059 / 7 / 595 / 9.01 / 67.82
Csa3M416130 / Chr3:19778725-19783358 / 6 / 370 / 6.02 / 41.62
Csa6M295950 / Chr6:14172987-14176086 / 7 / 480 / 5.64 / 53.77
Csa7M016620 / Chr7:947172-954937 / 7 / 480 / 5.18 / 53.49
Csa3M824820 / Chr3:32565481-32568966 / 6 / 326 / 7.18 / 36.01
Csa7M075640 / Chr7:5286284-5295509 / 6 / 257 / 5.47 / 27.77
Csa2M007420 / Chr2:1282957-1291326 / 9 / 1054 / 6.91 / 115.88
Csa1M470260 / Chr1:16892368-16901886 / 6 / 373 / 7.06 / 40.70
Csa6M505870 / Chr6:25742923-25746516 / 4 / 332 / 5.77 / 37.09
Csa3M816680 / Chr3:31670432-31675422 / 4 / 449 / 6.32 / 49.08
Csa1M423250 / Chr1:15510527-15519308 / 6 / 810 / 6.44 / 88.54
Csa3M119460 / Chr3:6701042-6714733 / 6 / 2491 / 6.39 / 27.50
Csa4M006330 / Chr4:1079567-1081720 / 5 / 324 / 8.39 / 36.05
Csa3M881590 / Chr3:37187991-37197783 / 2 / 1964 / 5.04 / 21.44
Csa6M538710 / Chr6:28981119-28992910 / 3 / 515 / 8.65 / 56.92
Csa5M286070 / Chr5:11910175-11912510 / 2 / 152 / 9.51 / 16.59
Csa5M468480 / Chr5:16494316-16505234 / 4 / 319 / 5.54 / 35.29
Csa7M420840 / Chr7:16215200-16219724 / 6 / 433 / 8.90 / 49.10
Csa7M368210 / Chr7:13061713-13077920 / 4 / 1343 / 6.47 / 144.46
Csa1M004370 / Chr1:832026-838731 / 7 / 514 / 6.11 / 58.60
Csa3M849390 / Chr3:34809974-34816724 / 4 / 510 / 5.96 / 56.76
Csa7M451930 / Chr7:18837283-18846311 / 5 / 512 / 5.91 / 57.08
Csa1M652290 / Chr1:25966194-25979291 / 4 / 1130 / 6.00 / 125.77
Csa5M583340 / Chr5:20742157-20749201 / 5 / 1098 / 6.24 / 122.13
Csa2M036700 / Chr2:3826354-3834080 / 2 / 317 / 7.68 / 34.68
Csa1M418260 / Chr1:15140418-15144363 / 2 / 811 / 6.23 / 91.86
Csa7M433260 / Chr7:17371790-17376108 / 2 / 624 / 4.75 / 67.68
Csa5M208460 / Chr5:9242541-9256836 / 7 / 1107 / 5.26 / 121.54
Csa3M731100 / Chr3:27500408-27509078 / 4 / 1141 / 5.73 / 124.65
Csa3M002310 / Chr3:223136-234030 / 6 / 1053 / 7.77 / 116.60
Csa1M144290 / Chr1:9621664-9639260 / 2 / 960 / 5.05 / 108.07
Csa3M116870 / Chr3:6333156-6353121 / 3 / 1960 / 5.19 / 216.81
Csa2M285390 / Chr2:13480717-13497305 / 4 / 1624 / 8.27 / 176.90

Table S3 A catalog of WDR proteins in Arabidopsis. The bold WDRs were identified in this study.

Gene / Location / Number
of WDR / Deduced polypeptide
Length (aa) / PI / MW(KDa)
AT5G24520 / Chr5:8370587-8372847 / 2 / 341 / 4.48 / 37.89
AT1G12910 / Chr1:4394897-4396291 / 2 / 346 / 4.57 / 39.09
AT3G26640 / Chr3:9793220-9794457 / 3 / 346 / 4.48 / 39.09
AT1G29260 / Chr1:10224815-10226205 / 5 / 317 / 5.17 / 35.47
AT2G19540 / Chr2:8461757-8464534 / 4 / 469 / 4.68 / 51.40
AT3G15354 / Chr3:5169095-5172837 / 2 / 837 / 6.07 / 93.38
AT2G16780 / Chr2:7281462-7283646 / 5 / 415 / 4.40 / 46.70
AT1G53090 / Chr1:19783352-19786902 / 2 / 794 / 5.34 / 89.07
AT4G35050 / Chr4:16682580-16684848 / 5 / 424 / 4.30 / 47.98
AT1G18830 / Chr1:6489309-6494218 / 3 / 969 / 5.68 / 106.59
AT3G63460 / Chr3:23430642-23437374 / 2 / 1104 / 4.76 / 119.87
AT4G35370 / Chr4:16815138-16817544 / 3 / 433 / 4.46 / 47.73
AT5G58230 / Chr5:23556012-23558245 / 5 / 424 / 4.44 / 48.19
AT5G13480 / Chr5:4326473-4331971 / 6 / 653 / 8.17 / 73.21
AT5G15550 / Chr5:5058749-5062087 / 5 / 433 / 5.61 / 47.34
AT5G64630 / Chr5:25833174-25836384 / 5 / 487 / 6.41 / 54.11
AT2G32950 / Chr2:13977933-13983535 / 3 / 675 / 6.83 / 76.19
AT5G67320 / Chr5:26857154-26861119 / 7 / 613 / 5.95 / 69.77
AT4G32990 / Chr4:15920230-15922658 / 6 / 328 / 4.47 / 36.60
AT1G10580 / Chr1:3491281-3493737 / 4 / 573 / 7.08 / 65.13
AT5G27570 / Chr5:9734896-9736131 / 5 / 411 / 7.66 / 45.56
AT2G33340 / Chr2:14126217-14131153 / 4 / 525 / 6.03 / 56.73
AT5G43920 / Chr5:17672669-17675845 / 5 / 523 / 5.96 / 59.12
AT3G45620 / Chr3:16745882-16748156 / 2 / 515 / 8.69 / 58.02
AT2G19520 / Chr2:8455936-8459525 / 4 / 507 / 6.17 / 55.76
AT4G22910 / Chr4:12012677-12016011 / 4 / 483 / 8.81 / 52.84
AT4G29730 / Chr4:14558985-14562538 / 4 / 487 / 6.56 / 53.99
AT4G11110 / Chr4:6771605-6777225 / 4 / 1036 / 5.98 / 115.00
AT1G04510 / Chr1:1226575-1230827 / 4 / 523 / 6.61 / 56.87
AT2G46340 / Chr2:19022173-19027413 / 4 / 1029 / 7.36 / 114.69
AT4G18905 / Chr4:10359896-10363285 / 3 / 504 / 4.24 / 55.23
AT5G27945 / Chr5:9976007-9977601 / 5 / 428 / 6.19 / 47.69
AT5G08560 / Chr5:2770874-2774672 / 3 / 589 / 6.95 / 65.83
AT5G25150 / Chr5:8677091-8682205 / 6 / 669 / 7.12 / 74.41
AT2G26060 / Chr2:11102301-11105355 / 7 / 352 / 4.58 / 39.13
AT5G13840 / Chr5:4468508-4471466 / 3 / 481 / 9.28 / 52.38
AT5G52820 / Chr5:21401349-21404385 / 8 / 473 / 8.53 / 52.90
AT4G28450 / Chr4:14061459-14064708 / 5 / 452 / 9.93 / 51.36
AT4G00090 / Chr4:34091-36880 / 3 / 430 / 8.63 / 46.55
AT5G27080 / Chr5:9529603-9531081 / 4 / 466 / 8.40 / 51.71
AT2G41500 / Chr2:17304130-17307426 / 7 / 554 / 5.43 / 61.84
AT2G20330 / Chr2:8772687-8775614 / 4 / 648 / 5.58 / 71.20
AT5G05970 / Chr5:1795294-1799527 / 3 / 782 / 7.07 / 84.76
AT5G56130 / Chr5:22722482-22725095 / 4 / 315 / 6.58 / 35.37
AT5G26900 / Chr5:9463752-9465086 / 5 / 444 / 8.66 / 49.23
AT2G47410 / Chr2:19448139-19457294 / 6 / 1520 / 7.07 / 171.53
AT5G53500 / Chr5:21725441-21729300 / 4 / 654 / 6.66 / 72.45
AT2G22040 / Chr2:9374438-9376260 / 5 / 313 / 7.71 / 35.95
AT1G49540 / Chr1:18333623-18337520 / 8 / 840 / 6.37 / 92.94
AT5G49430 / Chr5:20037338-20045454 / 6 / 1677 / 7.08 / 186.92
AT3G51930 / Chr3:19271624-19273064 / 5 / 415 / 9.47 / 45.28
AT4G33260 / Chr4:16041067-16043457 / 5 / 447 / 8.18 / 49.50
AT4G34380 / Chr4:16438524-16440641 / 5 / 495 / 9.72 / 54.31
AT1G64610 / Chr1:23998725-24001950 / 4 / 647 / 5.44 / 72.30
AT3G27640 / Chr3:10232248-10235621 / 4 / 535 / 8.29 / 59.40
AT4G33270 / Chr4:16044352-16046792 / 5 / 457 / 8.58 / 50.55
AT4G02730 / Chr4:1207725-1209287 / 6 / 333 / 8.22 / 36.30
AT5G54200 / Chr5:21993349-21997076 / 4 / 825 / 7.79 / 93.02
AT4G11920 / Chr4:7160276-7163382 / 5 / 475 / 9.07 / 52.33
AT3G18140 / Chr3:6212596-6214785 / 5 / 305 / 6.97 / 34.31
AT1G20540 / Chr1:7112611-7115362 / 1 / 351 / 4.90 / 39.04
AT1G47610 / Chr1:17504783-17505964 / 5 / 351 / 9.77 / 38.71
AT4G29830 / Chr4:14597661-14599300 / 6 / 321 / 6.24 / 34.26
AT4G32551 / Chr4:15707481-15713662 / 5 / 969 / 7.57 / 106.52
AT1G76260 / Chr1:28610265-28613263 / 2 / 350 / 5.39 / 39.05
AT3G08850 / Chr3:2686583-2695300 / 2 / 1344 / 6.65 / 147.96
AT3G09080 / Chr3:2776259-2781785 / 4 / 1026 / 6.24 / 112.20
AT1G80710 / Chr1:30333423-30335871 / 3 / 516 / 8.74 / 57.78
AT1G18080 / Chr1:6222201-6224151 / 7 / 327 / 7.81 / 35.75
AT5G24320 / Chr5:8284436-8288039 / 4 / 698 / 8.16 / 77.32
AT4G34460 / Chr4:16477106-16479528 / 7 / 377 / 7.25 / 41.01
AT5G64730 / Chr5:25873112-25875267 / 5 / 299 / 7.50 / 33.07
AT5G50120 / Chr5:20382615-20383874 / 7 / 388 / 6.95 / 42.50
AT5G23430 / Chr5:7893671-7900387 / 5 / 837 / 8.78 / 91.43
AT5G08390 / Chr5:2701013-2707273 / 5 / 839 / 8.22 / 91.61
AT1G49040 / Chr1:18139234-18148981 / 6 / 1187 / 5.54 / 131.59
AT5G54520 / Chr5:22146514-22149120 / 4 / 457 / 8.15 / 50.61
AT5G16750 / Chr5:5504346-5509343 / 10 / 876 / 5.84 / 96.70
AT3G42660 / Chr3:14751280-14755701 / 3 / 953 / 6.28 / 104.85
AT1G48630 / Chr1:17981773-17983327 / 7 / 326 / 7.12 / 35.80
AT3G18130 / Chr3:6210904-6212433 / 7 / 326 / 7.14 / 35.83
AT3G20740 / Chr3:7248809-7252452 / 2 / 369 / 6.17 / 41.26
AT3G49660 / Chr3:18413613-18415579 / 7 / 317 / 6.50 / 34.82
AT2G26490 / Chr2:11267942-11269623 / 7 / 465 / 8.98 / 51.44
AT3G18950 / Chr3:6536715-6538658 / 5 / 473 / 8.45 / 52.13
AT2G32700 / Chr2:13866772-1387257 / 5 / 806 / 7.43 / 87.70
AT1G48870 / Chr1:18072325-18074457 / 4 / 593 / 5.47 / 66.42
AT5G50230 / Chr5:20448370-20450881 / 6 / 509 / 6.17 / 56.43
AT5G42010 / Chr5:16802280-16805013 / 3 / 709 / 5.68 / 79.08
AT3G15470 / Chr3:5216262-5220218 / 4 / 883 / 7.11 / 97.41
AT1G49450 / Chr1:18305541-18307557 / 6 / 471 / 6.72 / 52.50
AT3G50390 / Chr3:18702074-18703619 / 7 / 469 / 8.63 / 51.20
AT1G24530 / Chr1:8693227-8694705 / 5 / 418 / 9.94 / 45.94
AT1G24130 / Chr1:8534183-8535430 / 7 / 415 / 8.53 / 45.80
AT5G02430 / Chr5:526592-529839 / 3 / 905 / 7.59 / 99.89
AT2G37670 / Chr2:15797384-15800536 / 4 / 903 / 8.55 / 101.34
AT5G27030 / Chr5:9508718-9515407 / 3 / 1134 / 7.31 / 125.56
AT3G15880 / Chr3:5364081-5372293 / 3 / 1137 / 7.24 / 124.24
AT1G15750 / Chr1:5414817-5421033 / 3 / 1131 / 7.25 / 124.30
AT3G16830 / Chr3:5731534-5737772 / 3 / 1131 / 7.28 / 124.76
AT1G80490 / Chr1:30260903-30267038 / 3 / 1120 / 7.14 / 124.09
AT1G78070 / Chr1:29355022-29358993 / 1 / 449 / 4.61 / 50.67
AT5G56190 / Chr5:22742005-22745177 / 1 / 447 / 4.71 / 50.36
AT1G36070 / Chr1:13466911-13470936 / 1 / 418 / 5.49 / 47.25
AT3G13340 / Chr3:4331531-4335073 / 1 / 447 / 4.64 / 50.62
AT1G55680 / Chr1:20804592-20807940 / 1 / 445 / 4.60 / 50.31
AT4G25440 / Chr4:13006727-13009608 / 3 / 430 / 7.36 / 46.66
AT5G40880 / Chr5:16378895-16381335 / 3 / 472 / 9.18 / 51.30
AT5G51980 / Chr5:21113650-21116197 / 4 / 443 / 7.69 / 47.99
AT5G49200 / Chr5:19947796-19949055 / 2 / 419 / 8.65 / 46.07
AT3G62770 / Chr3:23218633-23221232 / 2 / 425 / 7.52 / 46.80
AT3G56440 / Chr3:20926001-20928988 / 1 / 391 / 6.98 / 43.89
At5G05150 / Chr5:1524841-1526199 / 1 / 374 / 8.47 / 41.67
At2G40810 / Chr2:17032464-17034600 / 2 / 393 / 7.07 / 43.83
AT1G52360 / Chr1:19499343-19505757 / 4 / 970 / 4.60 / 109.77
AT3G15980 / Chr3:5411292-5418493 / 5 / 930 / 4.60 / 105.01
AT1G79990 / Chr1:30084114-30092238 / 4 / 1135 / 4.72 / 128.63
AT4G35140 / Chr4:16723714-16726636 / 2 / 496 / 5.27 / 55.49
AT4G38480 / Chr4:18003916-18006140 / 2 / 471 / 4.33 / 52.61
AT1G52730 / Chr1:19642490-19645134 / 4 / 343 / 6.27 / 37.75
AT3G15610 / Chr3:5290847-5293090 / 4 / 341 / 6.45 / 37.36
AT1G15470 / Chr1:5315507-5317863 / 4 / 333 / 6.08 / 36.34
AT1G03060 / Chr1:712473-726891 / 2 / 3601 / 6.03 / 400.89
AT4G02660 / Chr4:1159667-1174321 / 1 / 3527 / 6.34 / 393.32
AT5G01770 / Chr5:294313-301984 / 2 / 1336 / 6.46 / 147.64
AT3G13300 / Chr3:4303874-4310213 / 2 / 1344 / 6.01 / 145.72
AT3G13290 / Chr3:4297381-4303508 / 2 / 1340 / 6.05 / 145.88
AT2G21390 / Chr2:9152006-9156878 / 5 / 1218 / 6.91 / 136.47
AT1G62020 / Chr1:22919534-22924024 / 5 / 1216 / 6.96 / 136.56
AT1G27470 / Chr1:9540113-9544292 / 2 / 810 / 7.15 / 89.78
AT4G07410 / Chr4:4201249-4205224 / 3 / 815 / 6.51 / 90.27
AT1G11160 / Chr1:3733139-3739842 / 6 / 1021 / 6.97 / 112.53
AT1G54710 / Chr1:20416770-20421035 / 1 / 927 / 5.56 / 100.51
AT1G03380 / Chr1:835785-840940 / 1 / 959 / 7.02 / 104.58
AT2G18900 / Chr2:8188257-8192489 / 2 / 804 / 7.02 / 88.01
AT3G26480 / Chr3:9687273-9690827 / 1 / 764 / 6.67 / 84.41
AT5G19920 / Chr5:6733216-6737373 / 2 / 656 / 8.48 / 74.06
AT2G01330 / Chr2:158277-160858 / 8 / 611 / 6.41 / 66.39
AT3G18060 / Chr3:6183773-6187074 / 8 / 609 / 5.78 / 66.04
AT3G53390 / Chr3:19792531-19796753 / 3 / 558 / 7.81 / 59.88
AT2G37160 / Chr2:15608828-15612934 / 3 / 573 / 8.26 / 61.41
AT2G05720 / Chr2:2147192-2148215 / 3 / 276 / 8.81 / 31.10
AT4G21130 / Chr4:11274296-11276299 / 4 / 479 / 6.02 / 53.58
AT4G05410 / Chr4:2742932-2745627 / 3 / 504 / 6.18 / 56.52
AT1G17720 / Chr1:6093208-6098383 / 5 / 501 / 5.61 / 56.27
AT4G15900 / Chr4:9023728-9027666 / 6 / 486 / 9.30 / 54.01
AT3G16650 / Chr3:5671067-5675324 / 6 / 479 / 9.79 / 53.57
AT2G01470 / Chr2:211970-214472 / 3 / 393 / 5.31 / 42.79
AT5G50550 / Chr5:20576870-20578913 / 3 / 383 / 6.07 / 42.08
AT5G52250 / Chr5:21216855-21218226 / 3 / 385 / 4.83 / 42.00
AT5G23730 / Chr5:8005234-8006630 / 3 / 368 / 6.76 / 40.00
AT2G30910 / Chr2:13153214-13157107 / 2 / 378 / 6.90 / 41.96
AT2G31300 / Chr2:13346122-13349980 / 2 / 378 / 7.09 / 41.82
AT3G19590 / Chr3:6805631-6808593 / 3 / 340 / 8.52 / 37.87
AT1G49910 / Chr1:18479025-18481475 / 3 / 339 / 7.95 / 37.67
AT1G80670 / Chr1:30320576-30323780 / 4 / 349 / 8.48 / 38.27
AT1G15850 / Chr1:5453245-5454088 / 3 / 140 / 4.74 / 15.06
AT5G66240 / Chr5:26466204-26468448 / 3 / 331 / 5.87 / 36.52
AT5G14530 / Chr5:4684620-4686909 / 3 / 330 / 6.42 / 36.63
AT2G46280 / Chr2:19003426-19005536 / 4 / 328 / 7.00 / 36.39
AT2G46290
AT2G30050
AT3G01340
AT1G19750
AT1G27840
AT2G45540
AT1G58230
AT2G46560
AT4G29380
AT4G11270
AT4G01860
AT5G05570
AT3G44530
AT1G08190
AT4G00800
AT3G21540
AT4G04940
AT1G15440
AT2G40360
AT5G10940
AT4G34280
AT3G18860
AT3G05090
AT5G43930
AT3G49400
AT3G50590
AT3G44600
AT5G11240
AT1G64350
AT5G58760
AT2G25420
AT5G21040
AT4G03020
AT3G10530
AT5G14050
AT2G47990
AT1G73720
AT3G21060
AT1G29320
AT3G49180
AT5G60940
AT2G47790
AT3G52190
AT1G71840
AT4G21520
AT5G45760
AT1G65030
AT2G43770
AT5G63010
AT2G19430
AT1G69400
AT1G03110
AT4G29860
AT1G61210
AT2G34260
AT3G56900
AT2G16405
AT5G50970
AT3G33530
AT5G07590
AT1G04140
AT1G19485
AT3G06880
AT4G18900
AT4G21530
AT4G30840
AT4G35560
AT5G17370
AT5G24710
AT5G50650 / Chr2:19005771-19007879
Chr2:12824451-12826684
Chr3:127355-128994
Chr1:6826997-6830063
Chr1:9693008-9696364
Chr2:18757522-18772831
Chr1:21566148-21579123
Chr2:19115371-19125856
Chr4:14458822-14464859
Chr4:6854364-6859664
Chr4:801345-808060
Chr5:1656566-1663929
Chr3:16115682-16121484
Chr1:2567475-2573393
Chr4:336945-345714
Chr3:7585946-7590856
Chr4:2511028-2517193
Chr1:5306061-5309514
Chr2:16852880-16856690
Chr5:3448636-3454231
Chr4:16406998-16410785
Chr3:6501668-6508553
Chr3:1418261-1423664
Chr5:17676816-17681000
Chr3:18319249-18324482
Chr3:18771019-18779502
Chr3:16164935-16169262
Chr5:3582540-3586986
Chr1:23881512-23883297
Chr5:23730500-23733698
Chr2:10816749-10821053
Chr5:7144922-7147129
Chr4:1331399-1335297
Chr3:3285892-3288880
Chr5:4533261-4535301
Chr2:19636867-19638678
Chr1:27725002-27729931
Chr3:7377434-7380213
Chr1:10255321-10258343
Chr3:18229749-18232004
Chr5:24522978-24525812
Chr2:19570964-19573027
Chr3:19353972-19357074
Chr1:27022218-27024551
Chr4:11447434-11450443
Chr5:18561111-18563195
Chr1:24156770-24158675
Chr2:18134061-18136001
Chr5:25281714-25283176
Chr2:8415159-8417960
Chr1:26086961-26089020
Chr1:749246-751963
Chr4:14601698-14605796
Chr1:22564495-22571907
Chr2:14465849-14468531
Chr3:21066124-21069483
Chr2:7105752-7108972
Chr5:20736337–20739295
Chr3:14085354-14093449
Chr5:2401712-2403731
Chr1:1075545-1080630
Chr1:6746812-6751507
Chr3::2170516-2175686
Chr4:10356465-10359078
Chr4:11450743-11455862
Chr4:15017831-15019709
Chr4:16881562-16886878
Chr5:5721801-5724719
Chr5:8459148-8467920
Chr5:20610212-20612061 / 5
5
6
4
5
1
2
2
2
2
3
5
6
1
1
10
6
4
3
5
1
7
6
1
2
1
2
2
2
4
1
4
5
1
2
3
5
3
1
3
6
2
2
8
3
3
3
7
2
2
1
3
3
5
4
2
2
1
4
3
4
5
2
5
2
3
6
3
5
4 / 355
302
302
450
451
3001
2604
2513
1494
1446
1308
1124
1058
980
1913
955
910
900
753
757
783
760
753
752
892
1614
631
615
326
557
740
539
493
536
546
530
511
547
468
438
429
392
398
407
425
360
345
343
343
367
314
427
386
1181
353
447
482
512
1345
458
790
815
1261
461
777
361
1049
529
1377
383 / 6.92
5.53
5.98
9.26
8.80
5.73
6.76
6.34
7.06
6.06
5.83
5.54
6.84
4.86
5.08
7.31
6.94
6.29
4.93
5.72
6.59
5.26
7.23
8.40
6.63
8.42
6.61
6.86
4.70
8.01
6.33
8.35
6.73
10.14
5.28
10.15
7.00
4.48
7.73
5.50
9.13
4.53
7.42
4.11
4.52
6.97
6.91
7.36
4.45
5.58
4.65
5.33
5.87
8.83
4.67
5.94
8.98
9.39
6.27
4.59
7.65
8.06
7.07
4.26
4.60
4.53
7.35
8.77
6.71
6.07 / 39.00
32.64
32.63
50.00
49.75
328.09
292.78
275.52
168.12
159.38
144.75
122.66
116.49
110.28
211.99
106.06
102.22
100.47
85.38
83.83
88.17
84.27
82.20
81.86
99.72
176.30
70.98
66.88
35.70
62.83
83.16
60.55
55.41
60.26
60.99
58.90
57.72
60.14
52.27
48.15
47.06
43.07
43.77
43.79
47.54
39.50
37.45
37.89
37.53
40.07
34.76
46.44
41.99
129.17
38.43
48.49
53.97
57.98
146.90
51.85
85.67
90.10
141.41
51.08
87.42
39.35
116.36
58.99
148.07
42.08

Table S4List of characterized Arabidopsis WDR proteins

TAIR ID / Gene Name / Function / Function REF
AT1G76260 / DWA2 / NegativeRegulators in ABA Signal Transduction / Lee et al., 2010
AT2G19430 / DWA1
AT1G61210 / DWA3 / Negative regulator of ABA responses and involved in protein degradation / Lee et al., 2011
AT3G62770 / ATG18a / Involved in formation ofautophagosomes during starvation and senescence / Xiong et al., 2005
AT1G03380 / ATG18g / Up-regulated under drought condition
AT1G54710 / ATG18h / Responses to a combination of heat and droughtstress
AT2G30910 / APRC1A / Control leaf cell morphogenesis / Li et al., 2003
AT2G31300 / APRC1B
AT2G01330 / AIP1-1 / Regulation of the Pollen-Specific Actin-DepolymerizingFactor LlADF1 / Allwood et al., 2006
AT3G18060 / AIP1-2
AT2G46280 / TRIP-1 / Regulation of plant growth and development by BR / Jiang and Clouse, 2001
AT4G07410 / PCN / Functions in the auxin pathway to regulate embryonic body plan and meristem organization / Xiang et al., 2011
AT2G01470 / SEC12 / Endoplasmic Reticulum and Golgi Transport / Bar-Peled and Raikhel, 1997
AT1G27840 / ATCSA-1 / Critical factors for UV-B tolerance and genomic integrity / Biedermann and Hellmann, 2010
AT5G58760 / DDB2
AT3G13300 / VCS / Control leaf blade development / Deyholos et al., 2003
AT3G19590 / BUB 3.1 / Regulation of cell division and function as spindle assembly checkpoint (SAC) protein / Caillaud et al., 2009
AT3G21060 / RBL / Control Floral Transitionand Plant Development / Jiang et al., 2011
AT1G49540 / ELP2 / Accelerator of defense gene induction / DeFraia et al., 2010
AT2G34260 / WDR55 / Regulator of male and female gametogenesis and seed development / Bjerkan et al., 2012
AT3G44600 / CYP71 / Involved in chromatin assembly and histone modification in plants / Li and Luan, 2011
AT5G05970 / NEDD1 / Functions in microtubule organization during cell division / Zeng et al., 2009
AT5G21040 / FBX2 / Negatively regulates Pi starvation responses / Chen et al., 2008
AT3G18140 / LST8-1 / Function in regulating amino acid accumulation and the
synthesis of myo-inositol and raffinose during plant adaptation to long days / Moreau et al., 2012
AT3G49660 / WDR5a / Represses the Floral Transition / Jiang et al., 2009
AT2G41500 / LIS / Restricts gametic cell fatein the female gametophyte / Groß-Hardt et al., 2007
AT4G15900 / PRL1 / Regulation of sugar responseswith isoprenoid metabolism / Flores-Pe´ rez et al., 2010
AT4G21130 / EMB2271 / Criticalfor embryogenesis and gametogenesis / Li et al., 2010
AT4G05410 / YAO
AT1G18080 / RACK1A / Involvement in protein translation and its regulation by abscisic acid / Guo et al., 2011
AT1G48630 / RACK1B
AT3G18130 / RACK1C
AT5G64630 / FAS2 / Maintains the cellular organizationof apical meristems / Kaya et al., 2001
AT3G44530 / HIRA / A direct role for HIRA in knox generepression and the establishment of determinacy during leaf formation / Phelps-Durr et al., 2005
AT3G20740 / FIE1 / Regulation ofembryo and endosperm growth / Rodrigues et al., 2008
AT5G56130 / TEX1 / Involved in the production of endogenous and exogenous small interfering RNA / Jauvion et al., 2010
AT3G49180 / RID3 / Involved in shootregeneration / Tamaki et al., 2009
AT3G52190 / PHF1 / Enables the endoplasmic reticulum exit of a high-affinity phosphate
transporter / Gonza´ lez et al., 2005
AT5G67320 / HOS15 / Involvement in histonedeacetylation and cold tolerance / Zhu et al., 2008
AT5G58230 / MSI1 / Regulation of the cell cycle but notcell fate during male gametogenesis and negativeRegulation of the Response to Drought Stress / Chen et al., 2008
Alexandre et al., 2009
AT2G19520 / MSI4 / Regulation offlowering time / Pazhouhandeh et al., 2011
AT4G29730 / MSI5 / Cell cycle-related repressor gene / Breuil-Broyer et al., 2004
AT4G29830 / SKI8 / Context-dependent dual role inmRNA synthesis and turnover / Dorcey et al., 2012
AT1G29260 / PEX7 / Function in the maintenanceof glyoxysomal and leaf peroxisomal functions / Hayashi et al., 2005
AT4G34460 / AGB1 / Involved in vegetativeand reproductive development as well as in light andoxidative stress responses / Chen and Brandizzi, 2012
AT1G73720 / SMU1 / Regulation of pre-mRNA splicing and multiple aspectsof development / Chung et al., 2009
AT1G03110 / TRM82 / tRNA modification gene / Chen et al., 2010
AT5G13480 / FY / Involved in the development of dormancy and ABA sensitivity / Jiang et al., 2012
AT2G47990 / SWA1 / Involvedin 18S ribosomal RNA biogenesis / Shi et al., 2005
AT4G33270 / CDC20.1 / Regulation of mitotic cell cycle / Kevei et al., 2011
AT4G33260 / CDC20.2
AT4G22910 / FZR2 / Determinant of endoreduplication and cell expansion / Larson-Rabin et al., 2009
AT5G52250 / EFO1 / Regulation ofvegetative development and flowering / Wang et al., 2011
AT5G23730 / EFO2
AT2G46340 / SPA1 / Represses not onlyred, far-red and blue light responses, but also acts to suppress light signaling indarkness / Fittinghoff et al., 2006
AT4G11110 / SPA2 / Primarily regulatesseedling development in darkness and has little function inlight-grown seedlings or adult plants
AT3G15354 / SPA3 / Predominantly regulate elongation growth in adultplants
AT1G53090 / SPA4
AT4G29860 / TANMEI / Regulation of embryo development / Yamagishi et al., 2005
AT1G12910 / LWD1 / RegulateArabidopsis Photoperiodic Flowering / Wu et al., 2008
AT3G26640 / LWD2
AT5G24520 / TTG1 / Regulation of trichome formation, anthocyanin production, seed coat mucilage production and position-dependent root hair spacing / Zhang et al., 2003
AT3G08850 / Raptor1 / Essentialfor early embryo development / Deprost et al., 2005
AT1G80710 / DRS1 / Regulators of drought sensitivity / Lee et al., 2010
AT3G05090 / LRS1 / Regulator of lateral root development
AT2G33340 / MAC3A / Function in theregulation of plant innate immunity / Monaghan et al., 2009
AT1G04510 / MAC3B
AT1G03060 / SPI / Regulation of cell morphogenesis / Saedler et al., 2009
AT2G32950 / COP1 / Represses photomorphogenesis and induces skotomorphogenesis in the dark / Liu et al., 2012
AT5G16750 / TOZ / Regulation ofdivision planes and embryo development / Griffith et al., 2007
AT1G15440 / PWP2 / Plays an essential role inArabidopsis development / Matsui and Ohme-Takagi, 2010
AT1G49040 / SCD1 / Functions in polarized vesicle trafficking during plantcytokinesis and cell expansion / Falbel et al., 2003
AT4G29380 / VPS15 / Functions in pollen development and germinationby regulating PI3P biosynthesis / Xu et al., 2011
AT1G08190 / VPS41 / Plays role in theproper function of the post-Golgi traffi cking network andsupport membrane traffi cking to vacuoles. / Niihama et al., 2009
AT1G15750 / TPL / Involved inauxin and jasmonate signal transduction, meristem
maintenance, and defense responses / Causier et al., 2012
AT3G15880 / TPR4 / Functions in maintain the stem cells in the shoot apical meristem / Sitaraman et al., 2008
AT1G80490 / TPR1 / Regulation of apical embryonic fate / Long et al., 2006
AT3G16830 / TPR2
AT5G27030 / TPR3
AT4G32551 / LUG / Regulation of embryo and floral development / Sitaraman et al., 2008
AT2G32700 / LUH
AT4G21530 / APC4 / Critical for both female gametogenesis and embryogenesis / Wang et al., 2012

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Table S5 A catalog of duplicated genes in duplicated segmental regions and non-duplicated segmental regions in Arabidopsis.

Duplicated genes in duplicated segmental regions / Duplicated genes in non-duplicated segmental regions
AT2G40810/AT3G56440 / AT1G20540/AT1G76260
AT3G19590/AT1G49910 / AT1G27470/AT4G07410
AT2G21390/AT1G62020 / AT2G18900/AT3G26480
AT1G52360/AT3G15980 / AT2G01470/AT5G50550
AT4G32990/AT2G26060 / AT1G54710/AT1G03380
AT1G52730/AT3G15610 / AT2G30050/AT3G01340
AT3G18950/AT1G49450 / AT1G19750/AT1G27840
AT4G25440/AT5G51980 / AT4G35140/AT4G38480
AT1G48630/AT3G18130 / AT2G30910/AT2G31300
AT3G53390/AT2G37160 / AT3G13300/AT3G13290
AT4G35050/AT2G16780 / AT1G18830/AT3G63460
AT2G19520/AT4G29730 / AT2G47410/AT5G49430
AT3G15354/AT1G53090 / AT2G01330/AT3G18060
AT3G26640/AT1G12910 / AT2G46280/AT2G46290
AT5G01770/AT3G08850 / AT5G23430/AT5G08390
AT1G04510/AT2G33340 / AT1G11160/AT1G61210
AT1G03060/AT4G02660 / AT2G22040/AT3G18140
AT1G55680/AT3G13340 / AT4G02730/AT3G49660
AT5G02430/AT2G37670 / AT2G41500/AT2G05720
AT1G64610/AT5G42010 / AT5G40880/AT5G49200
AT5G43930/AT1G04140 / AT4G15900/AT3G16650
AT4G21130/AT4G05410
AT4G35370/AT4G18905
AT4G33260/AT4G33270
AT5G27080/AT5G26900
AT4G22910/AT4G11920
AT5G52250/AT5G23730
AT5G19920/AT5G50970
AT5G66240/AT5G14530
AT1G78070/AT1G36070
AT1G15750/AT1G80490
AT5G27030/AT3G16830
AT5G43920/AT5G08560
AT4G32551/AT2G32700
AT5G54200/AT3G15470
AT5G53500/AT5G24320
AT1G24530/AT1G24130

Table S6 Specific primers used for 6 cucumber WDR genes used in semi-quantitative RT-PCR in this study.

CsWDR / Sense primer 5’-3’ / Antisense-primer 5’-3’
Csa7M012400 / CAGATACAACAGTGGGTC / CATTTGCCAGACCTGAAC
Csa5M580650 / GCTGATCATGCAGTTGCT / GATTCAACTCCTGTGCTG
Csa1M267200 / CTGCTTACTTCTCACCCT / CATATAAACCTGTCCCCC
Csa1M572400 / GAGAGGGAGGAATGCATA / TCACCTATCAAGCCAGTC
Csa5M431540 / GAACCATTCCTGGCATTG / TTGGGGTGGTCGAGGTAAAT
Csa6M492270 / CTGGACCGGGTACAAATA / CAAAACAGGCAGCGATCT

Table S7 One-to-one orthologous relationships between Arabidopsis and cucumber

Synteny Block / AtWDR gene / Chr / CsWDR gene / Chr
1 / AT1G49040 / 1 / Csa1M042240 / 1
2 / AT1G61210 / 1 / Csa1M072490 / 1
3 / AT1G11160 / 1 / Csa1M072490 / 1
4 / AT1G08190 / 1 / Csa1M144290 / 1
5 / AT1G78070 / 1 / Csa2M308350 / 2
6 / AT1G04510 / 1 / Csa3M134680 / 3
7 / AT1G58230 / 1 / Csa3M124950 / 3
8 / AT1G52360 / 1 / Csa3M516570 / 3
9 / AT1G79990 / 1 / Csa3M516570 / 3
10 / AT1G53090 / 1 / Csa4M637680 / 4
11 / AT1G15440 / 1 / Csa4M004810 / 4
12 / AT1G64350 / 1 / Csa4M051460 / 4
13 / AT1G10580 / 1 / Csa5M601580 / 5
14 / AT1G20540 / 1 / Csa6M358120 / 6
15 / AT1G76260 / 1 / Csa6M358120 / 6
16 / AT1G49450 / 1 / Csa7M075570 / 7
17 / AT1G17720 / 1 / Csa7M451930 / 7
18 / AT1G03110 / 1 / Csa7M387800 / 7
19 / AT1G17720 / 1 / Csa3M849390 / 3
19 / AT1G17720 / 1 / Csa7M451930 / 7
20 / AT2G18900 / 2 / Csa1M423250 / 1
21 / AT2G16405 / 2 / Csa2M249890 / 2
22 / AT2G20330 / 2 / Csa3M611350 / 3
23 / AT2G45540 / 2 / Csa3M124950 / 3
24 / AT2G46560 / 2 / Csa3M119460 / 3
25 / AT2G47410 / 2 / Csa3M166260 / 3
26 / AT2G32700 / 2 / Csa3M131970 / 3
27 / AT2G33340 / 2 / Csa3M134680 / 3
28 / AT2G46340 / 2 / Csa5M150990 / 5
29 / AT2G16780 / 2 / Csa7M012400 / 7
30 / AT2G21390 / 2 / Csa7M004160 / 7
31 / AT2G47990 / 2 / Csa7M390220 / 7
32 / AT3G18060 / 3 / Csa1M024940 / 1
33 / AT3G16830 / 3 / Csa2M033270 / 2
34 / AT3G06880 / 3 / Csa3M159430 / 3
35 / AT3G01340 / 3 / Csa3M829070 / 3
36 / AT3G27640 / 3 / Csa3M839810 / 3
37 / AT3G56900 / 3 / Csa3M816680 / 3
38 / AT3G15354 / 3 / Csa4M637680 / 4
38 / AT3G15470 / 3 / Csa4M638360 / 4
39 / AT3G08850 / 3 / Csa4M325540 / 4
40 / AT3G09080 / 3 / Csa4M307950 / 4
41 / AT3G44530 / 3 / Csa6M152320 / 6
42 / AT3G16650 / 3 / Csa6M319790 / 6
43 / AT3G49660 / 3 / Csa6M497140 / 6
44 / AT3G18860 / 3 / Csa7M075640 / 7
44 / AT3G18950 / 3 / Csa7M075570 / 7
45 / AT3G62770 / 3 / Csa7M395790 / 7
46 / AT4G21520 / 4 / Csa1M071910 / 1
47 / AT4G29730 / 4 / Csa1M588520 / 1
48 / AT4G29830 / 4 / Csa1M590260 / 1
49 / AT4G32551 / 4 / Csa2M379340 / 2
50 / AT4G35560 / 4 / Csa3M002310 / 3
51 / AT4G11270 / 4 / Csa4M050140 / 4
52 / AT4G22910 / 4 / Csa5M156190 / 5
53 / AT4G11920 / 4 / Csa5M156190 / 5
54 / AT4G11110 / 4 / Csa5M150990 / 5
55 / AT4G15900 / 4 / Csa6M319790 / 6
56 / AT4G34460 / 4 / Csa7M267960 / 7
57 / AT4G35050 / 4 / Csa7M012400 / 7
57 / AT4G35140 / 4 / Csa7M016620 / 7
58 / AT4G02730 / 4 / Csa7M388400 / 7
59 / AT4G03020 / 4 / Csa7M391250 / 7
60 / AT5G50970 / 5 / Csa1M051780 / 1
61 / AT5G63010 / 5 / Csa1M004880 / 1
62 / AT5G45760 / 5 / Csa1M470260 / 1
63 / AT5G60940 / 5 / Csa1M039850 / 1
64 / AT5G43930 / 5 / Csa2M169190 / 2
65 / AT5G27030 / 5 / Csa2M033270 / 2
66 / AT5G43920 / 5 / Csa3M611890 / 3
67 / AT5G49430 / 5 / Csa3M166260 / 3
68 / AT5G40880 / 5 / Csa3M842110 / 3
69 / AT5G14530 / 5 / Csa3M838780 / 3
70 / AT5G56130 / 5 / Csa3M061010 / 3
71 / AT5G54520 / 5 / Csa3M088960 / 3
72 / AT5G01770 / 5 / Csa4M325540 / 4
73 / AT5G25150 / 5 / Csa4M652030 / 4
74 / AT5G02430 / 5 / Csa4M295520 / 4
75 / AT5G07590 / 5 / Csa5M167100 / 5
76 / AT5G23430 / 5 / Csa5M580650 / 5
77 / AT5G05570 / 5 / Csa5M208460 / 5
78 / AT5G05970 / 5 / Csa6M008800 / 6
79 / AT5G58760 / 5 / Csa6M185330 / 6
80 / AT5G66240 / 5 / Csa6M505870 / 6
81 / AT5G67320 / 5 / Csa6M492270 / 6
82 / AT5G64630 / 5 / Csa6M483440 / 6
82 / AT5G64730 / 5 / Csa6M486680 / 6