Supplement Fig. 1 Sequence multiple alignments and comparisons for chemerin of common pig (Sus scrofa EU660865) and Xiang pig (Sus scrofa domestica). Overall, this pig chemerin shares 96.67% homology with the known common pig chemerin. The Xiang pig sequence is available at GenBank with the accession number HQ538820
Supplement Fig. 2 Amino acid alignment of human, cattle, rabbit and Xiang pig chemerin. Multiple sequence alignment was generated using Clustal X. Putative signal peptide (SP) was predicted using SignalP 3.0 Server, the putative nuclear localization signal (NLS) profile was by Motif Scan and the putative phosphorylation sites (P1 and P2) was by NetPhos 2.0 Server.The putative cut site highlighted in box was predicted by ProP 1.0 Server
Supplement Fig. 3 Phylogenntic analysis of Xiang pig chemerin. The unrooted tree was constructed using neighbor-joining (NG) method based on the alignment of the complete amino acid sequences of known vertebrate chemerin by Clustal X
Supplement Table 1 Primers used for RT-PCR
Genes / Primers / Sequences (5’-3’) / Size (bp) / TA1 (°C)Chemerin / Forward / AGTTCCACAAGCACCCACCC / 148 / 59
Reverse / GCTTTCTTCCAGTCCCTCTT
ChemerinR / Forward / CTAACCATCCCTGCCTCCCT / 138 / 57
Reverse / ATCCTGACCACCCTGCCTTC
β-actin (P) 2 / Forward / TGCGGGACATCAAGGAGAAG / 216 / 64
Reverse / AGTTGAAGGTGGTCTCGTGG
PFK / Forward / AGTCCAGCTACCTGAACATCG / 166 / 58
Reverse / ACTTCTAACACAAACGTCCTCTG
PK / Forward / CTCCTGGAAGAAACGCCTTATT / 145 / 61
Reverse / CACCTTGCAGTAGTTGGAACC
FABP / Forward / ACCAAGCCTACTACCATCATCG / 112 / 60
Reverse / CCTCGTCGAACTCTATTCCCAG
ACO / Forward / CAGGAAGAGCAAGGAAGTGG / 189 / 58
Reverse / CCTTTCTGGCTGATCCCATA
CPT / Forward / GCACACCAGGCAGTAGCTTT / 107 / 59
Reverse / CAGGAGTTGATTCCAGACAGGTA
ISR / Forward / ATGGGCTTCGGGAGAGGAT / 121 / 60
Reverse / GGATGTCCATACCAGGGCAC
β-actin (M) 3 / Forward / GACCTCTATGCCAACACA / 270 / 55
Reverse / TCAGTAACAGTCCGCCTA
1 TA, Tannealing temperature.
2β-actin (P), β-actin for pig
3β-actin (M), β-actin for mouse
Supplement Table 2 Subcellular location of chemerin predicted by Predotar, WoLF PSORT and TargetP 1.1 Server.
Programs / Subcellular location and valuePredotar / ER1 / mitochondrial / elsewhere / prediction
0.99 / 0.02 / 0.01 / ER
WoLF PSORT / Extr2 / Mito3 / Pero4 / prediction
26.0 / 1.0 / 2.0 / extr
TargetP 1.1 Server / SP5 / mTP6 / Other / prediction
0.903 / 0.062 / 0.045 / SP
1ER: endoplasmic reticulum
2Extra: extracellular
3Mito: mitochontrial
4Pero: peroxisome
5SP: secretory pathway signal peptide
6mTP: mitochondrial targeting peptide
Supplement Table 3 Nuclear location and function motif (or structural annotation) predicted by InterProScan Gene3D 9.2.0 and ELM.
Programs / Nuclear location and function motifInterProScan / Nuclear Transport Factor 2
ELM / The classical bipartite nuclear localisation signal1
The classical monopartite nuclear localisation signal1
The monopartite nuclear localisation signal1
PIKK phosphorylation site2
USP7 binding motif 2
FHA phosphopeptide ligands2
1Nuclear location motif
2Nuclear function motif: functional cell compartment at nucleus