Supplementary Table 1:
Table S1: Cell divisons [%] inC.persicum protoplast culturesembedded in alginate after FDA staining compared toa control with isolation buffer and a control with the solvent of FDA (acetone).
Buffer / Acetone / FDA1 week / 12.8 ± 18.0 / 10.6 ± 14.8 / 11.0 ± 15.7
2 weeks / 18.7 ± 25.0 / 18.1 ± 23.7 / 20.0 ± 27.1
n / 9 / 9 / 9
Each mean value (± standard deviation) represents data from n different Petri dishes
The analysis of deviance, following a generalized linear model (quasibinomial) revealed no difference in the cell division between the FDA stained, acetone treated and the control protoplasts at the 5% significance level p=0.8086 (1 week, alginate); p=0.9586 (2 weeks, alginate).
Supplementary Table 2:
Table S2: Cell divisons [%] inC.persicum protoplast cultures embedded in alginate after staining with different scopoletin concentrations
Scopoletin [ng/mL culture medium]0 / 8 / 16
1 week / 41.1 ± 12.2 / 32.4 ± 11.3 / 32.7 ± 4.4
2 weeks / 21.2 ± 3.6 / 19.7 ± 3.9 / 19.8 ± 0.3
N / 3 / 3 / 3
Each mean value (± standard deviation) represents data from three different Petri dishes from one protoplast isolation.
The analysis of deviance, following a generalized linear model (quasibinomial revealed no difference in the cell division between the scopoletin stained and the control protoplasts at the 5% significance level: p=0.4113 (1 week, alginate); p=0.842 (2 weeks, alginate).
Supplementary Table 3:
Table S3:Absolute DNA contents of C. coum estimated by measurements with two different internal standards (given are means and standard deviations of four measurements)
Internal reference standard / DNA content [pg/2C] / CV [%]Secale cereale (16.01 pg/ 2C) / 14.79 + 0.13 / 3.33 + 1.09
Vicia faba (26.21 pg/ 2C) / 14.63 + 0.06 / 3.95 + 0.44